Difference between revisions of "Team:CSU Fort Collins/NoteBook/Aug"
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<div class = 'Info'><ul><li>Colony PCRed transformed 21 I7+Luc colonies</li> | <div class = 'Info'><ul><li>Colony PCRed transformed 21 I7+Luc colonies</li> | ||
<img src="https://static.igem.org/mediawiki/2016/d/da/CSU_LuCSlr0168Aug11.png"> | <img src="https://static.igem.org/mediawiki/2016/d/da/CSU_LuCSlr0168Aug11.png"> | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <div class = 'Days'><h5>8/12/16:</h5></div> | ||
| + | <div class = 'Info'><ul><li>Phosphorylated Q1 and Q2 for ligase cycling</li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <div class = 'Days'><h5>8/15/16:</h5></div> | ||
| + | <div class = 'Info'><ul><li>Performed ligase cycling reaction on phosphorylated Q block mixture</li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | |||
| + | <div class = 'Days'><h5>8/19/16:</h5></div> | ||
| + | <div class = 'Info'><ul><li>Performed colony PCR on Slr0168+Luc</li> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/7/73/CSU_CyanoAug19.jpg"> | ||
</ul> | </ul> | ||
</div> | </div> | ||
Revision as of 15:45, 17 October 2016
CSU iGEM
August
8/6/16:
- Performed Colony PCR on Slr0118+Luc transformed colonies
- Ran PCR results on a 1% gel
- Gel had no bands
8/8/16:
- Digested I712013 and Luc
- Ligated I712013 and Luc
- Transformed ligation mixture
8/11/16:
- Colony PCRed transformed 21 I7+Luc colonies
8/12/16:
- Phosphorylated Q1 and Q2 for ligase cycling
8/15/16:
- Performed ligase cycling reaction on phosphorylated Q block mixture
8/19/16:
- Performed colony PCR on Slr0168+Luc
