Difference between revisions of "Team:Tongji Shanghai/Model"

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             <h2>Project</h2>
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             <h2>Achievment</h2>
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             <p>Our project is our story.</p>
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             <p>Why we deserve a medal.</p>
 
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               <h4>Notebook<span class="head-line"></span></h4>
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               <h4>Modeling<span class="head-line"></span></h4>
 
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            <!-- Fudan ~ Tongji -->  
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        <!-- Materials Synthesis -->  
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       <h3 class="classic-title" style="margin-top:0px"><span>Notebooks</span></h3>
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             <ul style="margin-left:0px; font-size:16px">We have synthesized the AuNRs (gold nanorods) and detoxified them for photothermal therapy, also known as optical hyperthermia or photothermal ablation, which is an emerging strategy for treating solid tumors. Gold nanoparticles are capable of confining resonant photons, further inducing coherent surface plasmon oscillation of their conduction band electrons.<br><br>
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             <ul style="margin-left:0px; font-size:16px;">Team Fudan need tet-on or tet-of promoter to get tetO element and miniCMV as the promoter to drive their genes of interest. Vice professor Wenwen Jia in our school works on apparent heredity field, happens to have tet-on kit for his work. We made contact with Pro. Wenwen Jia and gained 3μl. <br><br>
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             For non-invasive therapy, near infrared (NIR) radiation is chosen because it penetrates tissue more deeply. And we find that AuNRs with a strong SPR(surface plasmon resonance) in the NIR region can show intense absorption of light in the NIR region, also biocompatibility. Even more, it can accumulate in tumor tissue via passive targeting phenomena. PEG can be used to increase biocompatibility, suppress immunogenic responses and decrease adsorption. </ul>
+
             We also asked him for the associating kit that responses to panmycin and send the signal to promoter. Team Fudan used these two kits to PCR and continued their experiments. At the same time, Team Fudan had provided great help either. They built their kit ahead of us, and they generously provided almost all of the materials that we gonna need for kit submitting.(15 ml of chloromycetin powder,8 chloramphenicol resistance plates,80ml chloramphenicol resistance LB,Enzyme of XbaI and SpeI each 3μl)  </ul>
 
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            <ul style="margin-left:0px; font-size:16px">Cancer thermotherapy realized by depositing heat into tumor in a minimally invasive way is a promising alternative to the conventional therapies for cancer treatment. It’s a therapeutic tool to eradicate cancer tumor with minimum toxic effects. To make it better, we plan to improve its ability to target to tumor cells and thermosensitivity of tumor cells. Furthermore, we hope it can reflect the the treatment process. Here, we provide an approach to optimize its tumor targeting and thermosensitivity of tumor cells by hTert promoter and heat shock protein 70(hsp70) prompter and the following tumor suppressor p53. Additionally, the luciferase following p53 makes it a reporter system.
 
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       <h3 class="classic-title" style="margin-top:20px"><span>Cell Experiments</span></h3>
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       <h3 class="classic-title" style="margin-top:20px"><span>Basic Parts</span></h3>
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      <!--<h4>Taipei Workshop in NYMU<span class="head-line"></span></h4><br>
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            <ul style="margin-left:0px; font-size:16px;">In order to test the toxicity and killing efficiency of our system, we have designed in vitro cytotoxicity test and in vitro photo thermal effect test. Cell viability is tested with cellTiter-Glo luminescent cell viability assay, which can reflect the ATP concentration of cells.<br><br>
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          <h4>BBa_K1922001<span class="head-line"></span></h4><br>
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            </ul>
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          <ul style="margin-left:0px; font-size:16px;">Approximately 80% of cancers are immortalized by constitutive activa-tion of telomerase to maintain telomeres throughout rapid cellular prolif-eration. HTert promoter is the promoter we cloned from human cell, which can drive the transcription of telomerase in human. We hoped that it can promote the transcription of the certain gene following it in tumor cells specifically, instead of in normal cells. </ul>
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          <h4>BBa_K1922003<span class="head-line"></span></h4><br>
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          <ul style="margin-left:0px; font-size:16px;">The cells of all tissues react to various stresses through the quick synthesization of HSPs.As the most important HSPs family. HSP70 has the highest temperature sensitivity and conservation. Having synthesized human hsp70 promoter, we hope it can drive the transcription of selected gene after heat shock at certain tem-perature. From the literature we finally set the preform condition as 42C for 1h </ul>         
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            <ul style="margin-left:0px; font-size:16px;">Previous work has shown that golden nanorods is harmless to cells under 100μg/ml, and perform well when exposed to NIR. The feasibility of in vivo near-infrared OPTT is demonstrated after infected plasmids in tumor-bear mice by direct injection. Near-infrared OPTT was performed extra corporally using a portable wave diode laser.</ul>
 
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    <h4>Style Switcher</h4>
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    <span>12 Predefined Color Skins</span>
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    <ul class="colors-list">
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      <li>
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        <a onClick="setActiveStyleSheet('blue'); return false;" title="Blue" class="blue"></a>
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      </li>
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      <li>
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        <a onClick="setActiveStyleSheet('sky-blue'); return false;" title="Sky Blue" class="sky-blue"></a>
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        <a onClick="setActiveStyleSheet('jade'); return false;" title="Jade" class="jade"></a>
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        <a onClick="setActiveStyleSheet('green'); return false;" title="Green" class="green"></a>
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        <a onClick="setActiveStyleSheet('pink'); return false;" title="Pink" class="pink"></a>
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        <a onClick="setActiveStyleSheet('red'); return false;" title="Red" class="red"></a>
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    </ul>
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    <span>Top Bar Color</span>
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    <select id="topbar-style" class="topbar-style">
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      <option value="1">Light (Default)</option>
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      <option value="2">Dark</option>
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      <option value="3">Color</option>
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    </select>
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    <span>Layout Style</span>
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    <select id="layout-style" class="layout-style">
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      <option value="1">Wide</option>
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      <option value="2">Boxed</option>
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    <span>Patterns for Boxed Version</span>
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    <ul class="bg-list">
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      <li>
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        <a href="#" class="bg1"></a>
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        <a href="#" class="bg9"></a>
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        <a href="#" class="bg10"></a>
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        <a href="#" class="bg11"></a>
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    </ul>
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Revision as of 17:01, 17 October 2016

Tongji_Shanghai-2016.igem.org Tongji Shanghai

Achievment

Why we deserve a medal.

Favorite Parts

    Team Fudan need tet-on or tet-of promoter to get tetO element and miniCMV as the promoter to drive their genes of interest. Vice professor Wenwen Jia in our school works on apparent heredity field, happens to have tet-on kit for his work. We made contact with Pro. Wenwen Jia and gained 3μl.

    We also asked him for the associating kit that responses to panmycin and send the signal to promoter. Team Fudan used these two kits to PCR and continued their experiments. At the same time, Team Fudan had provided great help either. They built their kit ahead of us, and they generously provided almost all of the materials that we gonna need for kit submitting.(15 ml of chloromycetin powder,8 chloramphenicol resistance plates,80ml chloramphenicol resistance LB,Enzyme of XbaI and SpeI each 3μl)

Basic Parts

BBa_K1922001


    Approximately 80% of cancers are immortalized by constitutive activa-tion of telomerase to maintain telomeres throughout rapid cellular prolif-eration. HTert promoter is the promoter we cloned from human cell, which can drive the transcription of telomerase in human. We hoped that it can promote the transcription of the certain gene following it in tumor cells specifically, instead of in normal cells.


BBa_K1922003


    The cells of all tissues react to various stresses through the quick synthesization of HSPs.As the most important HSPs family. HSP70 has the highest temperature sensitivity and conservation. Having synthesized human hsp70 promoter, we hope it can drive the transcription of selected gene after heat shock at certain tem-perature. From the literature we finally set the preform condition as 42C for 1h

Style Switcher

12 Predefined Color Skins
Top Bar Color Layout Style Patterns for Boxed Version