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| Line 552: |
Line 552: |
| | <div style="height: 200px; width: 400px;"> | | <div style="height: 200px; width: 400px;"> |
| | </br></br> | | </br></br> |
| − | <p> Extracted Genomic DNA from MG1655 and 6803 (See Protocol)</p> | + | <p> </p> |
| | </div> | | </div> |
| | <div class="text">June 6th</div> | | <div class="text">June 6th</div> |
| Line 561: |
Line 561: |
| | <div style="height: 200px; width: 400px;"> | | <div style="height: 200px; width: 400px;"> |
| | </br></br> | | </br></br> |
| − | <p> Stuff that happened on day 7 </p>
| + | <ul> |
| | + | <li>Performed gDNA Extraction on E. Coli MG1655 and Synechocystis 6803</li> |
| | + | </ul> |
| | </div> | | </div> |
| | <div class="text">June 7th</div> | | <div class="text">June 7th</div> |
| Line 570: |
Line 572: |
| | <div style="height: 200px; width: 400px;"> | | <div style="height: 200px; width: 400px;"> |
| | </br></br> | | </br></br> |
| − | <p> Stuff that happened on day 8 </p>
| + | <ul> |
| | + | <li>Designed PCR primers for <i>pgk</i>, <i>pck</i>, <i>fldA</i>, <i>petF</i>, ATP Synthase 1, and ATP Synthase 2</li> |
| | + | </ul> |
| | </div> | | </div> |
| | <div class="text">June 8th</div> | | <div class="text">June 8th</div> |
| Line 588: |
Line 592: |
| | <div style="height: 200px; width: 400px;"> | | <div style="height: 200px; width: 400px;"> |
| | </br></br> | | </br></br> |
| − | <p> Ran PCR for petF gene, fldA gene, and pdCas9 plasmid </p>
| + | <ul> |
| − | <p>Made gel and ran PCR product</p>
| + | <li>Primers Arrived</li> |
| − | <p>Extracted DNA from gel</p>
| + | <li>Ran PCR on ATP Synthase 2 , <i><i>pgk</i></i>, <i><i>fldA</i></i>, <i><i>petF</i></i>, and pdCas9 plasmid</li> |
| − | <p>Re-ran pdCas9 PCR</p>
| + | <li>Ran all PCR products on gel and recovered ATP Synthase 2, <i><i>fldA</i></i>, and <i><i>petF</i></i></li> |
| | + | <li>Ran gradient PCR on pdCas9 plasmid</li> |
| | + | </ul> |
| | </div> | | </div> |
| | <div class="text">June 10th</div> | | <div class="text">June 10th</div> |
| Line 619: |
Line 625: |
| | </br></br> | | </br></br> |
| | <p> Made and ran gel for pdCas9</p> | | <p> Made and ran gel for pdCas9</p> |
| − | <p>Extracted DNA</p> | + | <ul> |
| − | <p>Made agar plates</p> | + | <li>Ran PCR on ATP Synthase 1, <i><i>pgk</i></i>, and <i><i>pck</i></i></li> |
| − | <p>Ran golden gate one pot assembly for both genes</p> | + | <li>Performed gel extraction on yesterday’s ATP Synthase 2</li> |
| | + | <li>Ran <i><i>pck</i></i>, <i><i>pgk</i></i>, and yesterday’s gradient pdCas9 PCR products on gel and recovered all genes</li> |
| | + | <li>Performed gel extraction on pdCas9, <i>fldA</i>, <i>petF</i></li> |
| | + | <li>Ran PCR on psc101 and psc102 plasmid</li> |
| | + | <li>Ran psc101 and psc102 PCR products on gel - were recovered</li> |
| | + | <li>Ran gradient PCR on ATP Synthase 1</li> |
| | + | <li>Made agar plates (forgot the antibiotic)</li> |
| | + | <li>Ran golden gate one pot assembly for <i>fldA</i> <i>petF</i> genes and the pdCas9 plasmid</li> |
| | + | </ul> |
| | </div> | | </div> |
| | <div class="text">June 13th</div> | | <div class="text">June 13th</div> |
| Line 630: |
Line 644: |
| | <div style="height: 200px; width: 400px;"> | | <div style="height: 200px; width: 400px;"> |
| | </br></br> | | </br></br> |
| − | <p> Stuff that happened on day 14 </p>
| + | <ul> |
| | + | <li>Ran ATP Synthase 1 PCR products on gel - no bands seen</li> |
| | + | <li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li> |
| | + | <li>Ran PCR on ATP Synthase 1 (3rd times the charm)</li> |
| | + | <li>Ran PCR products on gel - WE SAW BANDS! ATP SYNTHASE 1 RECOVERED</li> |
| | + | <li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel Extraction</li> |
| | + | <li>Made new agar plates (with the chloramphenicol)</li> |
| | + | <li>Transformed the <i>petF</i>, <i>fldA</i> plasmids into competent cells</li> |
| | + | <li>Plated colonies on agar plates and set in incubator</li> |
| | + | </ul> |
| | </div> | | </div> |
| | <div class="text">June 14th</div> | | <div class="text">June 14th</div> |
| Line 639: |
Line 662: |
| | <div style="height: 200px; width: 400px;"> | | <div style="height: 200px; width: 400px;"> |
| | </br></br> | | </br></br> |
| − | <p> Stuff that happened on day 15 </p>
| + | <ul> |
| | + | <li>Checked for colonies (both genes were successful)</li> |
| | + | <li>Picked colonies and incubated overnight</li> |
| | + | <li>Ran yesterday’s PCR products on gel - bands were light but all were recovered</li> |
| | + | <li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li> |
| | + | <li>Reran PCR on <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction</li> |
| | + | <li>Ran PCR products on gel - all were recovered</li> |
| | + | <li>Gel Extraction for <i>pgk</i>, <i>pck</i>, psc101, and psc102 plasmid</li> |
| | + | </ul> |
| | </div> | | </div> |
| | <div class="text">June 15th</div> | | <div class="text">June 15th</div> |
Notebook
Reading about labwork is almost as fun as doing labwork
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1 / 30
Stuff that happened on day 1
June 1st
2 / 30
Stuff that happened on day 2
June 2nd
4 / 30
Stuff that happened on day 4
June 4th
5 / 30
Stuff that happened on day 5
June 5th
7 / 30
- Performed gDNA Extraction on E. Coli MG1655 and Synechocystis 6803
June 7th
8 / 30
- Designed PCR primers for pgk, pck, fldA, petF, ATP Synthase 1, and ATP Synthase 2
June 8th
9 / 30
Stuff that happened on day 9
June 9th
10 / 30
- Primers Arrived
- Ran PCR on ATP Synthase 2 , pgk, fldA, petF, and pdCas9 plasmid
- Ran all PCR products on gel and recovered ATP Synthase 2, fldA, and petF
- Ran gradient PCR on pdCas9 plasmid
June 10th
11 / 30
Stuff that happened on day 11
June 11th
12 / 30
Stuff that happened on day 12
June 12th
13 / 30
Made and ran gel for pdCas9
- Ran PCR on ATP Synthase 1, pgk, and pck
- Performed gel extraction on yesterday’s ATP Synthase 2
- Ran pck, pgk, and yesterday’s gradient pdCas9 PCR products on gel and recovered all genes
- Performed gel extraction on pdCas9, fldA, petF
- Ran PCR on psc101 and psc102 plasmid
- Ran psc101 and psc102 PCR products on gel - were recovered
- Ran gradient PCR on ATP Synthase 1
- Made agar plates (forgot the antibiotic)
- Ran golden gate one pot assembly for fldA petF genes and the pdCas9 plasmid
June 13th
14 / 30
- Ran ATP Synthase 1 PCR products on gel - no bands seen
- Gel Extraction for pgk, pck, psc101, and psc102 plasmid
- Ran PCR on ATP Synthase 1 (3rd times the charm)
- Ran PCR products on gel - WE SAW BANDS! ATP SYNTHASE 1 RECOVERED
- Reran PCR on pgk, pck, psc101, and psc102 plasmid because we did not get high enough concentration during gel Extraction
- Made new agar plates (with the chloramphenicol)
- Transformed the petF, fldA plasmids into competent cells
- Plated colonies on agar plates and set in incubator
June 14th
15 / 30
- Checked for colonies (both genes were successful)
- Picked colonies and incubated overnight
- Ran yesterday’s PCR products on gel - bands were light but all were recovered
- Gel Extraction for pgk, pck, psc101, and psc102 plasmid
- Reran PCR on pgk, pck, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction
- Ran PCR products on gel - all were recovered
- Gel Extraction for pgk, pck, psc101, and psc102 plasmid
June 15th
16 / 30
Stuff that happened on day 16
June 16th
17 / 30
Stuff that happened on day 17
June 17th
18 / 30
Stuff that happened on day 18
June 18th
19 / 30
Stuff that happened on day 19
June 19th
20 / 30
Stuff that happened on day 20
June 20th
21 / 30
Stuff that happened on day 21
June 21st
22 / 30
Stuff that happened on day 22
June 22nd
23 / 30
Stuff that happened on day 23
June 23rd
24 / 30
Stuff that happened on day 24
June 24th
25 / 30
Stuff that happened on day 25
June 25th
26 / 30
Stuff that happened on day 26
June 26th
27 / 30
Stuff that happened on day 27
June 27th
28 / 30
Stuff that happened on day 28
June 28th
29 / 30
Stuff that happened on day 29
June 29th
30 / 30
Stuff that happened on day 30
June 30th
1 / 31
Stuff that happened on day 31
July 1st
2 / 31
Stuff that happened on day 32
July 2nd
3 / 31
Stuff that happened on day 33
July 3rd
4 / 31
Stuff that happened on day 34
July 4th
5 / 31
Stuff that happened on day 35
July 5th
6 / 31
Stuff that happened on day 36
July 6th
7 / 31
Stuff that happened on day 37
July 7th
8 / 31
Stuff that happened on day 38
July 8th
9 / 31
Stuff that happened on day 39
July 9th
10 / 31
Stuff that happened on day 40
July 10th
11 / 31
Stuff that happened on day 41
July 11th
12 / 31
Stuff that happened on day 42
July 12th
13 / 31
Stuff that happened on day 43
July 13th
14 / 31
Stuff that happened on day 44
July 14th
15 / 31
Stuff that happened on day 45
July 15th
16 / 31
Stuff that happened on day 46
July 16th
17 / 31
Stuff that happened on day 47
July 17th
18 / 31
Stuff that happened on day 48
July 18th
19 / 31
Stuff that happened on day 49
July 19th
20 / 31
Stuff that happened on day 50
July 20th
21 / 31
Stuff that happened on day 51
July 21st
22 / 31
Stuff that happened on day 52
July 22nd
23 / 31
Stuff that happened on day 53
July 23rd
24 / 31
Stuff that happened on day 54
July 24th
25 / 31
Stuff that happened on day 55
July 25th
26 / 31
Stuff that happened on day 56
July 26th
27 / 31
Stuff that happened on day 57
July 27th
28 / 31
Stuff that happened on day 58
July 28th
29 / 31
Stuff that happened on day 59
July 29th
30 / 31
Stuff that happened on day 60
July 30th
31 / 31
Stuff that happened on day 61
July 31st
1 / 31
Stuff that happened on day 62
August 1st
2 / 31
Stuff that happened on day 63
August 2nd
3 / 31
Stuff that happened on day 64
August 3rd
4 / 31
Stuff that happened on day 65
August 4th
5 / 31
Stuff that happened on day 66
August 5th
6 / 31
Stuff that happened on day 67
August 6th
7 / 31
Stuff that happened on day 68
August 7th
8 / 31
Stuff that happened on day 69
August 8th
9 / 31
Stuff that happened on day 70
August 9th
10 / 31
Stuff that happened on day 71
August 10th
11 / 31
Stuff that happened on day 72
August 11th
12 / 31
Stuff that happened on day 73
August 12th
13 / 31
Stuff that happened on day 74
August 13th
14 / 31
Stuff that happened on day 75
August 14th
15 / 31
Stuff that happened on day 76
August 15th
16 / 31
Stuff that happened on day 77
August 16th
17 / 31
Stuff that happened on day 78
August 17th
18 / 31
Stuff that happened on day 79
August 18th
19 / 31
Stuff that happened on day 80
August 19th
20 / 31
Stuff that happened on day 81
August 20th
21 / 31
Stuff that happened on day 82
August 21st
22 / 31
Stuff that happened on day 83
August 22nd
23 / 31
Stuff that happened on day 84
August 23rd
24 / 31
Stuff that happened on day 85
August 24th
25 / 31
Stuff that happened on day 86
August 25th
26 / 31
Stuff that happened on day 87
August 26th
27 / 31
Stuff that happened on day 88
August 27th
28 / 31
Stuff that happened on day 89
August 28th
29 / 31
Stuff that happened on day 90
August 29th
30 / 31
Stuff that happened on day 91
August 30th
31 / 31
Stuff that happened on day 92
August 31st
1 / 30
Stuff that happened on day 93
September 1st
2 / 30
Stuff that happened on day 94
September 2nd
3 / 30
Stuff that happened on day 95
September 3rd
4 / 30
Stuff that happened on day 96
September 4th
5 / 30
Stuff that happened on day 97
September 5th
6 / 30
Stuff that happened on day 98
September 6th
7 / 30
Stuff that happened on day 99
September 7th
8 / 30
Stuff that happened on day 100
September 8th
9 / 30
Stuff that happened on day 101
September 9th
10 / 30
Stuff that happened on day 102
September 10th
11 / 30
Stuff that happened on day 103
September 11th
12 / 30
Stuff that happened on day 104
September 12th
13 / 30
Stuff that happened on day 105
September 13th
14 / 30
Stuff that happened on day 106
September 14th
15 / 30
Stuff that happened on day 107
September 15th
16 / 30
Stuff that happened on day 108
September 16th
17 / 30
Stuff that happened on day 109
September 17th
18 / 30
Stuff that happened on day 110
September 18th
19 / 30
Stuff that happened on day 111
September 19th
20 / 30
Stuff that happened on day 112
September 20th
21 / 30
Stuff that happened on day 113
September 21st
22 / 30
Stuff that happened on day 114
September 22nd
23 / 30
Stuff that happened on day 115
September 23rd
24 / 30
Stuff that happened on day 116
September 24th
25 / 30
Stuff that happened on day 117
September 25th
26 / 30
Stuff that happened on day 118
September 26th
27 / 30
Stuff that happened on day 119
September 27th
28 / 30
Stuff that happened on day 120
September 28th
29 / 30
Stuff that happened on day 121
September 29th
30 / 30
Stuff that happened on day 122
September 30th
1 / 31
Stuff that happened on day 93
October 1st
2 / 31
Stuff that happened on day 94
October 2nd
3 / 31
Stuff that happened on day 95
October 3rd
4 / 31
Stuff that happened on day 96
October 4th
5 / 31
Stuff that happened on day 97
October 5th
6 / 31
Stuff that happened on day 98
October 6th
7 / 31
Stuff that happened on day 99
October 7th
8 / 31
Stuff that happened on day 100
October 8th
9 / 31
Stuff that happened on day 101
October 9th
10 / 31
Stuff that happened on day 102
October 10th
11 / 31
Stuff that happened on day 103
October 11th
12 / 31
Stuff that happened on day 104
October 12th
13 / 31
Stuff that happened on day 105
October 13th
14 / 31
Stuff that happened on day 106
October 14th
15 / 31
Stuff that happened on day 107
October 15th
16 / 31
Stuff that happened on day 108
October 16th
17 / 31
Stuff that happened on day 109
October 17th
18 / 31
Stuff that happened on day 110
October 18th
19 / 31
Stuff that happened on day 111
October 19th
20 / 31
Stuff that happened on day 112
October 20th
21 / 31
Stuff that happened on day 113
October 21st
22 / 31
Stuff that happened on day 114
October 22nd
23 / 31
Stuff that happened on day 115
October 23rd
24 / 31
Stuff that happened on day 116
October 24th
25 / 31
Stuff that happened on day 117
October 25th
26 / 31
Stuff that happened on day 118
October 26th
27 / 31
Stuff that happened on day 119
October 27th
28 / 31
Stuff that happened on day 120
October 28th
29 / 31
Stuff that happened on day 121
October 29th
30 / 31
Stuff that happened on day 122
October 30th
31 / 31
Stuff that happened on day 122
October 31st
