Abstract

Through past entries in iGEM Giant Jamboree, we have learned a lot in synthetic biology and genetic engineering. Especially, we have come to face on several occasions the extent to which the general public fears synthetic biology and genetic engineering.

When we brainstormed our ideas for this year’s project, we wanted to contribute to the society with synthetic biology by applying them to therapeutics and health. This was the basis for the development of Noro-catcher. This project ultimately aims to administrate recombinant organisms inside the patient digestive system for its application. Through our project, we had to face the question that was fostered by our team’s continuous history of engaging with the general public: How far do the general public believe in genetic engineering, and, for example, would they be willing to entrust their health to recombinant organisms? One way around this question is of course to make our device as safe as possible. We tried this approach by adding a domain to swiftly remove the recombinant organisms, addressing the issue.

We continued our HP activities and had more opportunities to meet people from different walks of life. From such experiences, we began to understand that these fears in the general public arise from lack of knowledge on the matter. By educating them to increase their knowledge on genetic engineering, would people’s fear be soothed? In other words, would providing the general public with adequate knowledge on genetic engineering improve their perception on our activities? In order to test this hypothesis, we conducted a student-teacher workshop and provided informational seminar on genetic engineering.

From the feedbacks obtained after the seminar, it was shown that education has been very effective in reducing fears in students about genetic engineering. It suggests just two hours of informative lecture radically reduced their fears, and drastically improved their opinion. Their stance against genetic engineering swung far more than we had expected.

Thus our hypothesis was proven; to educate the public on science reduces their fears.

Our immediate and rather optimistic conclusion was however upturned. After discussing and analyzing the results within our team, doubts began to arise that our methods might have significant flaws.

As a student of synthetic biology, we cannot brand that the danger on the usage of recombinant organisms in human body systems are insignificantly small. However, students who listened to our informative lectures expressed a surprisingly accepting stance on recombinant organisms. It might be that this indicates a major flaw in our methods and analysis.

As we continued our analysis, we came across a deeply relevant psychological effect.

When applying this view on our human practice project, it suggests that it makes people prone to overestimation despite their incomplete knowledge on synthetic biology, and hinder proper risk assessment on the application of genetic engineering.

Results

Engage scientists as well as non-scientists

We held many activities to introduce experts as well as non-experts on our projects as well as synthetic biology. These practices not only helped gain recognition on our projects and our study, but also provided valuable opportunity to learn about people’s stance towards our projects and synthetic biology in general.

Below are our team’s engagements, only including the ones from this year

<1-1 Engaging the general public>

-November Festival at Kyoto University 11/20/2015-11/24/2015

-May Festival at University of Tokyo 05/15/2016-05/16/2016

<1-2 Engaging scientists>

-The 88th annual meetings of the Genetics Society of Japan 09/08/2016

<1-3 Public Relations>

-SPEC (Students Project for Enhancing Creativity) 01/27/2015

1-1) Poster session in November Festival and May Festival

Aim

Raise the general public’s recognition on genetic engineering and iGEM, and promote understanding for our project

Method

We performed a poster session in two of the most prestigious school festivals of Japan, Kyoto-U’s November Festival and U-Tokyo’s May Festival, where many people come visit.

Results

Many people visited the iGEMKyoto’s booths, and we were able to raise awareness of iGEM, our project, and genetic engineering in general. At the same time, we were able to collect various opinions on our project.

Summary of team member analysis

-Participant’s point of interest in our project widely differs

-Participant’s knowledge on the matter widely varies

-Technical stories cannot be communicated easily. If we explain starting with the conclusion, people tend to see our points, but we cannot know for sure how well they understood

-Some asked whether uptaking recombinant organisms are OK

-Many congratulated us for our research well beyond our position as an undergraduate students, but there seemed to be few anticipation for the practicality

Fig1: iGEMKyoto's team member presenting our project to visitors of the University of Tokyo's school festival

Fig2 Our team member presenting our project to visitors of Kyoto University's school festival

1-2) The 88th annual meetings of the Genetics Society of Japan

Aim

Exchanging ideas and opinions with other iGEM teams, and with Genetics Society of Japan members (Who usually are not familiar with iGEM or synthetic biology)

Method

As one of the workshops in the meeting, we presented in a special introduction session for iGEM teams

Results

We exchanged ideas with many Genetics Society members and iGEMers Summary of team member analysis

-We were surprised that iGEM has more recognition among Japanese biologists than we had anticipated

-We received valuable advice on our study and presentation skills

Fig3 Presenting our projects to biologists in 88th Annual Meeting of the Genetics Society of Japan.

1-3) SPEC (Student Projects for Enhancing Creativity)

Through Kyoto University’s crowdfunding, we were given the opportunity to introduce our project to wide range of people. Through various media, we had done a wide range of activities to raise recognition for genetic engineering and synthetic biology. For example, our project was reported on national newspaper.

Fig4 Presenting our project to Kyoto University's school president in SPEC preliminary selection

Fig5 Our project was reported on national newspaper through SPEC

1-4) What we have learned from these events

We learned innumerous things from these experiences. What struck our team members the most was how much the general public’s knowledge on genetic engineering varied. There were people with considerable knowledge on the matter, even as far as CRISPR, while others lacked the most basic of knowledge such as the functions of DNA. And those with little knowledge held an abstract concern for genetic engineering, as they only know its name. In order for our biodevice to be realized, we need to address their concerns and gain their trust.

What We Have Done

We have done the following to address the deep-rooted concerns of the people for genetic engineering that we have come to face.

First of all, we tried to improve our project design by the addition of a domain to increase safety of our biodevice.

Second of all, we worked to engage the public on genetic engineering and synthetic biology. We hypothesized that the concerns for genetic engineering arise from the lack of knowledge on genetic engineering. To test this hypothesis, we conducted an informational lecture/discussion about genetic engineering to students of the age range 13 to 18 with various backgrounds. We tested whether or not invitation to deeper understanding of genetic engineering reduces their concerns.

2-1) Improvement of our biodevice

The Noro-catcher we developed binds to Norovirus(NoV) to combat NoV infections. We aim to apply this biodevice to removal of the NoV from intestinal system by orally administrating them. We plan to use sterilized Noro-catcher to be safer, but we still anticipated a strong resistance to its usage as the patient is essentially “eating” recombinant organisms. As noted above, we found that the general public fears genetic engineering, and we wanted to address their concerns by introducing safe-usage mechanisms that physically removes our Noro-catcher from human digestive systems. Visit our project page (LINK) for additional details.

2-2) Public engagement through informative lecture and discussion

In order to test the hypothesis that the concerns people hold for genetic engineering arises from the lack of knowledge on the matter, we held an informative lecture/discussion and analyzed the feedbacks submitted from the participants.

Informative lectures (10/08/2016, Kobe)

Aim

To provide an accurate and unbiased information about genetic engineering and to promote understanding for our project

Method

We conducted a small educational seminar about genetic engineering and iGEM for 20 minutes to 4 students of the Kobe Secondary School attached to Kobe University (12th grade M:1, 11th Grade M:1 8th grade F:2) and then facilitated a discussion about genetic engineering and our projects for about 2 hours, with their science teacher. We also conducted a survey at the end to see how our seminar affected their views on genetic engineering.

Lecture

We explained the fundamentals of genetic engineering and iGEM. We introduced them on the positive and negative aspects of genetic engineering, trying to be as unbiased as we can be.

Fig6 Lecture/discussion about genetic engineering conducted to jr. and sr. highschool students

Feedforward

We conducted a survey before our lectures to 68 students. As seen clearly from the figure, from oral administration to detection, the student’s resistance to recombinant organism application increased with the level of proximity the user must be in with the recombinant organisms. This was as expected.

Fig7 results of feedforward survey

Feedback

Because of typhoon, only four participated in the informative lectures and feedbacks. Results are shown below.

Are you more interested in genetic engineering?

All four participants answered “Strongly more interested”

Does genetic engineering feel more familiar?

All four participants answered “Much more familiar”

Do you think the lecture today would help you assess genetic engineering in your daily life?

Three out of four answered “Strongly helps” and one answered “Helps”

Even though we did anticipate a more welcoming stance than feedforward, the actual results far exceeded our expectation, with all of the participants expressing extremely welcoming stance towards genetic engineering.

In order to compare with the feedforward results, we also asked the four feedback participants “How has your stance on genetic engineering change before and after the workshop?” The result matched the feedforward results, with two out of four expressing concerns for recombinants organisms at first. After the workshop, all four answered that they can accept all of the oral administration/prevention/detection application of the biodivice, which is a dramatic shift in their opinion.

Fig8 free-topic discussion with participating students

2-3) A new concern

We contacted an expert on risk communication, pedagogy and psychology for an advice on the analysis of our HP activity. We found three additional points of concern.

• Those with only partial knowledge tend to overestimate their knowledge, and they might fail to properly recognize its risks
• Our societal position as students of a prestigious university and the jr./sr. high students' admiration for university students may affect their views. We may have failed our objective to "provide unbiased information so the public can make an informed decision" with this asymmetric lecturer/lecturee power balance
• They might have tried to match our anticipation by answering what we wanted to hear, and withholding their true opinions.

As the participants to our lecture/workshop were limited in number, we could only speculate these points, but our finding in these surveys still holds significance in iGEM teams' future Human Practice activities.

2-4) Conclusion

Based on our hypothesis that we may be able to improve public perception on genetic engineering, we held a lecture/discussion workshop on genetic engineering. The results were drastic. Even though the number of students were not many, we were able to affect the students’ opinions as all answered they can accept the usage of recombinant organisms. We thus proved our hypothesis. However, new concerns arose in that we may have given the students overconfidence in synthetic biology by only giving them partial knowledge on the matter.

There have been numerous studies conducted on this issue, but they have yet to agree on an omnipotent solution. iGEM teams, however, face this problem every time they engage the general public, usually with only limited knowledge on genetic engineering. We hope to discuss this topic with other iGEM teams, to learn best practices and experiences about improving public perceptions on synthetic biology.