Difference between revisions of "Team:Kyoto/Notebook 2015"

Latest revision as of 17:57, 19 October 2016

August
						1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	31

September
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30

8/14

Transformation

Li, Uchino, Kaneko

Sample Name	Sample Volume (µl)	Competent Cells/(µl)	Medium
RFP 0.5ng/µl	2	DH5α /20	LB(CP)
RFP 5ng/µl	2	DH5α /20	LB(CP)
RFP 10ng/µl	2	DH5α /20	LB(CP)
RFP 20ng/µl	2	DH5α /20	LB(CP)
RFP 50ng/µl	2	DH5α /20	LB(CP)

8/14

Gel Extraction

Li, Uchino, Kaneko

Lane	Restriction Enzyme Digestion Product	Volume/(µl)
1	10/1 PQ(3)	about 23.5
2	1kb ladder	6
3	10/1 PQ(3)	about 23.5
4	10/1 PQ(3)	about 24

Total volume of samples of lane 1~3 was 71.5µl and it was divided into three.

8/14

Transformation

Li, Uchino, Kaneko

Sample Name	Sample Volume	Competent Cells/(µl)	Medium
3C5	10µl	DH5α 20µl	LB(CP)
P+	10µl	DH5α 20µl	LB(CP)
P-	10µl	DH5α 20µl	LB(CP)
RBS	10µl	DH5α 20µl	LB(CP)

8/14

Liquid Culture

Li, Uchino, Iguchi, Kaneko

Name	medium
8/13 transformation RFP 20ng/µl colony1	LB(CP)
8/13 transformation part RFP colony1	LB(CP)
8/13 transformation part RFP colony2	LB(CP)
8/13 transformation part RFP colony3	LB(CP)
8/13 transformation part RFP colony4	LB(CP)
8/13 transformation part RFP colony5	LB(CP)
8/13 transformation part RFP colony6	LB(CP)
8/13 transformation part RFP colony7	LB(CP)
8/13 transformation part RFP colony8	LB(CP)
8/13 transformation part RFP colony9	LB(CP)
8/13 transformation part RFP colony10	LB(CP)
8/13 transformation part RFP colony11	LB(CP)

8/15

Miniprep

Li, Uchino, Notsu, Kaneko, Watanabe

DNA	Concentration/(µg/ml)	260/280	260/230
RFP1 /2µl	82.5	1.40	1.22
RFP2 /4µl	142.5	1.69	2.05
RFP3 /4µl	50	1.55	1.71
RFP4 /4µl	57.5	1.64	1.88
RFP6 /4µl	98.75	1.71	1.40
RFP(20) /4µl	76.25	1.67	1.32

8/15

Electrophoresis

Notsu, Kaneko

Lane	Restriction Enzyme Digestion Products
1	1kb ladder 3µl
2	RFP1 10µl
3	RFP2 10µl
4	RFP3 10µl
5	RFP4 10µl
6	RFP6 10µl
7	RFP(20) 10µl
8	PCR1 5µl
9	PCR2 5µl
10	PCR3 5µl
11	PCR4 5µl
12	PCR5 5 µl
13	1kb ladder 3µl

8/15

Restriction Enzyme Digestion

Uchino, Notsu, Kaneko

Sample	DNA/(µl)	EcoR1/(µl)	Pst1/(µl)	Buffer/(µl)	MilliQ/(µl)	Total/(µl)
8/15Miniprep PSB1C3-RFP1	24	1	1	5(H)	19	50
8/15Miniprep PSB1C3-RFP2	14	1	1	5(H)	29	50
8/15Miniprep PSB1C3-RFP3	40	1	1	5(H)	3	50
8/15Miniprep PSB1C3-RFP4	35	1	1	5(H)	8	50
8/15Miniprep PSB1C3-RFP6	20	1	1	5(H)	23	50

8/15

colony PCR

Uchino, Notsu, Kaneko, Watanabe

colony Primers
8/14 transformation pSB1C3-RFP1 VF+VR
8/14 transformation pSB1C3-RFP2 VF+VR
8/14 transformation pSB1C3-RFP3 VF+VR
8/14 transformation pSB1C3-RFP4 VF+VR
8/14 transformation pSB1C3-RFP1 VF+VR

8/15

PCR (Steps)

Uchino, Notsu, Kaneko, Watanabe

Steps	Temparature	Time	Cycle
Pre Denature	94℃	2min	1
Denature	94℃	30sec	30
Annealing	55℃	30sec	30
Extension	68℃	1min	30
Final elongation	4℃	7min	1
Final Holding	4℃	-	-

8/16

Gel Extraction

Li, Iguchi, Notsu, Kaneko, Watanabe

Lane	Restriction Enzyme Digestion Product	Volume/(µl)
1	1kb ladder	10
2	blank
3	8/15 digestion pSB1C3-RFP 1	55
4	8/15 digestion pSB1C3-RFP 2	55
5	8/15 digestion pSB1C3-RFP 3	36
6	8/15 digestion pSB1C3-RFP 4	36
7	8/15 digestion pSB1C3-RFP 6	36

8/16

Electrophoresis

Li, Iguchi, Notsu, Kaneko, Watanabe

Lane	sample/(µl)	Length(bp)
1	8/16 Gel Extraction RFP1 /1
2	8/16 Gel Extraction RFP2 /1
3	8/16 Gel Extraction RFP3 /1
4	8/16 Gel Extraction RFP4 /1
5	blank
6	8/16 pSB1C3-vector /1
7	8/16 pSB1C3-vector /1
8	1kladder

8/16

Ligation

Li, Iguchi, Notsu, Kaneko, Watanabe

Vector DNA /(µl)	insert DNA /(µl)	ligase /(µl)	10×buffer /(µl)	MilliQ /(µl)	Total /(µl)
pSB1C3 EPcut /1	RFP /4	0.5	1	3	9.5
pSB1C3 EPcut /1	RFP /2	0.5	1	5	9.5
pSB1C3 EPcut /1	RFP /4	0.5	1	3	9.5
pSB1C3 EPcut /1	RFP /4	0.5	1	3	9.5
pSB1C3 EPcut /1	-	0.5	1	7	9.5

8/17

Liquid Culture

Li

Name	medium
8/16 transformation pSB3C5 RFP1	LB(CP) /6
8/16 transformation pSB3C5 RFP2	LB(CP) /6
8/16 transformation pSB3C5 RFP1	LB(CP) /6

8/17

Transformation

Li

Sample Name	Sample Volume (µl)	Competent Cells/(µl)	Medium
pSB1C3 P+	2	DH5α /20	LB
pSB1C3 P-	2	DH5α /20	LB
pSB1C3 RBS	2	DH5α /20	LB
pSB3C5 RFP	2	DH5α /20	LB
8/16 ligation pSB1C3 RFP1	2	DH5α	LB

8/17

Electrophoresis

Iguchi

Lane	sample/(µl)	Length(bp)
1	1kbp ladder /3
2	blank
3	pSB1C3-RFP(ligate)1 /2
4	pSB1C3-RFP(ligate)2 /2
5	pSB1C3-RFP(ligate)3 /2
6	pSB1C3-RFP(ligate)4 /3
7	Vector (Ctrl) /5

8/18

Liquid Culture

Li, Iguchi, Notsu

Name	medium
INP [1] BBa_K811005	LB(CP) / 6ml
INP [2] BBa_K811005	LB(CP) / 6ml
INP [3] BBa_K811005	LB(CP) / 6ml
scfv04 [1] BBa_K875004	LB(CP) / 6ml
scfv04 [2] BBa_K875004	LB(CP) / 6ml
scfv04 [3] BBa_K875004	LB(CP) / 6ml
scfv06 [1] BBa__K875006	LB(CP) / 6ml
scfv06 [2] BBa_K875006	LB(CP) / 6ml
scfv06 [3] BBa_K875006	LB(CP) / 6ml

8/18

Miniprep

Li, Iguchi, Notsu

DNA	Concentration/(µg/ml)	260/280	260/230
8/17 Liquid Culture 3C5 [1] / 5µl	77	1.69	1.45
8/17 Liquid Culture 3C5 [2] / 2µl	40	1.67	1.17
8/17 Liquid Culture 3C5 [3] / 2µl	15	3.75	3.70

8/18

Electrophoresis

Li

Lane	sample/(µl)	Length(bp)
1	1kb ladder /1	-
2	[1] psb3c5(8/18 miniprep) /4.2	3807
3	[2] psb3c5(8/18 miniprep) /4.2	3807
4	[1'] psb3c5(8/18 miniprep) /4.2	3807
5	[2'] psb3c5(8/18 miniprep) /4.2	3807
6	[3'] psb3c5(8/18 miniprep) /4.2	3807

[img18]

8/18

Restriction Enzyme Digestion

Li, Iguchi, Notsu

Sample	DNA/(µl)	EcoR1/(µl)	Xba1/(µl)	Spe1/(µl)	Pst1/(µl)	Buffer/(µl)	BSA/(µl)	MilliQ/(µl)	Total/(µl)
	psb3c5(8/18miniprep) [1'], [2'], [3'] mixed / 50	1.0			1.0	5.6			56.6

8/19

Transformation

Li, Iguchi, Notsu

Sample Name	Sample Volume (µl)	Competent Cells/(µl)	Medium
BBa_ J61002 p+	2	DH5a /20	LB(Amp)
BBa_J61002 p-	2	DH5a /20	LB(Amp)
BBa_1A2 RBS	2	DH5a /20	LB(Amp)

Failed due to deactivation of Amp

8/19

MutationPCR

Templates /µl	Primer /µl	Primer STAR Max Premix /µl	MilliQ /µl	Total(µl)
scfv plasmid /0.1	m.scfv FW /1, m.scfvRV/1	25	22.9	50
scfv plasmid (nega) /0.1	0	5	4.9	10
(nega) /0	m.scfv FW/0.2, m.scfvRV/0.2	5	4.6	10

---30cycle---
98℃ 10''
55℃ 15''
72℃ 20''
----------------
4℃ holding

8/19

Electrophoresis

Notsu

Lane	sample/(µl)	Length(bp)
1	1kb ladder/2	-
2	scfv plasmid +primer(m.scfv FW, m.scfv RV) mutation PCR product /5	3600
3	scfv plasmid mutation PCR product /5	-
4	primer(m.scfv FW, m.scfv RV) mutation PCR product /5	-
5	psb3c5 EPcut product /5	2600(backborn), 1200(inseert)

[img22]

8/19

Concentration

Li, Notsu

Sample	OD600	Bacterial cells /ml
INP [1] K811005	0.643	5.14x10^8
scfv06 K875006	0.634	5.07x10^8
scfv04 K875004	0.618	4.94x10^8

1 OD 600 equals to 8x10^8 cells /ml

8/19

Miniprep

Li, Notsu

DNA	Concentration/(µg/ml)	260/280	260/230
scfv 04[1] K875004 /3µl	56	1.63	1.45
scfv 04[2] K875004 /3µl	56	1.63	1.45
scfv 04[3] K875004 /3µl	56	1.63	1.45
scfv 06[1] K875006 /3µl	56	1.63	1.45
scfv 06[2] K875006 /3µl	56	1.63	1.45
scfv 06[3] K875006 /3µl	56	1.63	1.45
INP [1] K811005 /3µl	56	1.63	1.45
INP [2] K811005 /3µl	56	1.63	1.45
INP [3] K811005 /3µl	56	1.63	1.45

There was a problem with an instrument

8/20

Transformation

Li, Uchino

Sample Name	Sample Volume (µl)	Competent Cells/(µl)	Medium
8/19 mutation PCR scfv plasmid (nega)	1	DH5a/ 20	LB(CP)

8/19 mutation PCR:
1µl extraction of scfv plasmid (nega)/10µl was folded 300 times.

8/20

Transformation

Li, Uchino

Sample Name	Sample Volume (µl)	Competent Cells/(µl)	Medium
8/19 mutation PCR scfv plasmid	1 [note:1]	DH5a/20	LB(AMP)
8/19 mutation PCR scfv plasmid (nega)	1 [note:2]	DH5a/20	LB(AMP)
BBa_J61002 p+	1	DH5a/20	LB(AMP)
BBa_J61002 p-	1	DH5a/20	LB(AMP)
BBa_1A2 RBS	1	DH5a/20	LB(AMP)

[note:1]
8/19 mutation PCR:
1µl extraction of scfv plasmid (nega)/50µl was folded 300 times.
[note:2]
8/19 mutation PCR:
1µl extraction of scfv plasmid (nega)/10µl was folded 300 times.

8/20

Electrophoresis

Li

Lane	sample/(µl)	Length(bp)
1	1kb ladder /1.5
2	9/19 miniprep scfv04 [1]
3	9/19 miniprep scfv04 [2]
4	9/19 miniprep scfv04 [3]
5	9/19 miniprep scfv06 [1]
6	9/19 miniprep scfv06 [2]
7	9/19 miniprep scfv06 [3]
8	9/19 miniprep INP [1]
9	9/19 miniprep INP [2]
10	9/19 miniprep INP [3]

[img26]

8/21

Colony PCR

Uchino

Colony	Primers
8/20 transformation RBS	VF+VR
8/20 transformation p+	VF+VR
8/20 transformation p-	VF+VR
8/20 dilution INP	VF+VR

8/21

PCR (Steps)

Uchino

Steps	Temparature	Time	Cycle
Pre	94℃	2min	1
De	94℃	30 sec	30
An	55℃	30 sec	30
Ex	68℃	1min	30
Final E	4℃	7min	1
Final Hold	4℃	-	-

Autoclaved distrilled water /23µl
2xQuick taq /25µl
10 pmol/µl primer (VF) /1µl
10 pmol/µl primer (VR) /1µl
Template DNA /0µl
Total 50µl

8/21

Electrophoresis

Li

Lane	sample/(µl)	Length(bp)
1	1kb ladder /2
2	8/21 colony PCR RBS /all
3	8/21 colony PCR p+ /all
4	8/21 colony PCR p- /all
5	8/21 colony PCR INP /all
6	8/21 colony PCR Nega /all

[img29-1]
weak band except INP -> retry
colony PCR and Electrophoresis were conducted under the same condition.
[img29-2]
RBS and INP success.
P+ and P- failed -> retry.

8/21

PCR (Steps)

Li

Steps	Temparature	Time	Cycle
Predenature	95℃	3min	1
Denature	98℃	20sec	25
Annealing	70℃	15sec	25
Extension	72℃	30sec	25
Final Elongation	72℃	5min	1
Final Holding	4℃	-	1

8/21

PCR (Target)

Li

Templates/(µl)	Primer1/(µl)	Primer2/(µl)	Buffer/(µl)	MilliQ/(µl)	Any Other/(µl)	Total/(µl)
scfv 8/20 transformation	cscfv.f /0.75	cscfv.r /0.75	2x KAPA Hifi /12.5	11		25
- (nega)	cscfv.f /0.3	cscfv.f /0.3	2x KAPA Hifi /5	4.4		10

8/21

Colony PCR

Li

Colony	Primers
8/20 transformation p+ [1]	VF+VR
8/20 transformation p+ [2]	VF+VR
8/20 transformation p+ [3]	VF+VR
8/20 transformation p- [1]	VF+VR
8/20 transformation p- [2]	VF+VR
8/20 transformation p- [3]	VF+VR
8/18 parts p+	VF+VR
8/18 parts p-	VF+VR

8/21

PCR (Steps)

Li

Steps	Temparature	Time	Cycle
pre	94℃	2min	1
de	94℃	30sec	30
an	55℃	30sec	30
ex	68℃	1min	30
F.elong	4℃	7min	1
F.hold	4℃	-	1

8/21

Liquid Culture

Li

Name	medium
strain	Medium/ml
8/20 transformation RBS	PG(AMP) /6
8/20 dilution INP7	PG(CP) /6
8/17 transformation 3c5, RFP	PG(CP) /6
8/20 transformation p+ [1]	PG(AMP) /6
8/20 transformation p- [1]	PG(AMP) /6

8/21

Electrophoresis

Li

Lane	sample/(µl)	Length(bp)
1	1kb ladder /1.5
2	8/21 cloning PCR scfv[1] /2
3	8/21 cloning PCR scfv[2] /2
4	nega /2
5	8/21 colony PCR p+ [1] /2
6	8/21 colony PCR p+ [2] /2
7	8/21 colony PCR p+ [3] /2
8	8/21 colony PCR p- [1] /2
9	8/21 colony PCR p- [2] /2
10	8/21 colony PCR p- [3] /2
11	8/21 colony PCR parts p+ /2
12	8/21 colony PCR parts p- /2

[img35]
2, 3, 4 cloning PCR failed.
1 forgot to put ladder
5, 7, 8, 9 strong band
6 weak
10 failed
P+[1] and p-[1] -> liquid culture

8/24

Ligation

Li

sample/µl	MilliQ (µl)	Buffer /µl	Ligase(µl)	Total(µl)
8/19 mutation PCR scfv /5	3.5	1	0.5	10

8/24

Liquid Culture

Li

Name	medium
8/20 transformation m.scfv colonyA /6	PG(AMP)

8/24

Miniprep

Li

DNA	Concentration/(µg/ml)	260/280	260/230
8/20 Liquid culture RBS	124.5	1.65	1.02
8/20 Liquid culture INP	147.8	1.66	0.98
8/20 Liquid culture 3c5.RFP	234.8	1.79	1.62
8/20 Liquid culture P+[1]	40.3	1.78	1.60
8/20 Liquid culture P-[1]	56.7	1.80	2.05

8/25

Miniprep

Li, Uchino, Notsu, Kaneko

DNA	Concentration/(µg/ml)	260/280	260/230
8/24 LIquid Culture m.scfv colonyA	-	-	-
8/24 LIquid Culture m.scfv colonyB	-	-	-

8/25

Electrophoresis

Lane	sample/(µl)	Length(bp)
1	1kb ladder /1.5
2	8/24 miniprep p+(J61002) /2
3	8/24 miniprep p-(J61002) /2
4	8/24 miniprep RFP (3c5) /2
5	8/24 miniprep INP (1c3) /2
6	8/24 miniprep RBS (1A2) /2

[img38]

8/25

Restriction Enzyme Digestion

Uchino

DNA/(µl)	EcoR1/(µl)	Xba1/(µl)	Spe1/(µl)	Pst1/(µl)	Buffer/(µl)	MilliQ/(µl)	Total/(µl)
p+/24.8			1	1	10xH /4	9.2	40
IINP /6.8		1		1	10xM /4	27.2	40
p- /17.6			1	1	10xH /4	16.4	40
RBS /8.0			1	1	10xH /4	26	40
RFP /4.3	1			1	10xH /4	29.7	40

8/25

Gel Extraction

Uchino, Notsu

Lane	Restriction Enzyme Digestion Product	Volume/(µl)
1	1kb ladder	6
2, 3	8/25 digestion p+	24 each
4, 5	8/25 digestion p-	24 each
6, 7	8/25 digestion RFP	24 each
8, 9	8/25 digestion INP	24 each
10, 11	8/25 digestion RBS	24 each

[img40]

8/25

Actvation check of Xba and Pst

Uchino

INP(µl)	Xba(µl)	Pst(µl)	10xM(µl)	BSA(µl)	MilliQ(µl)	Total(µl)
0.68	0.1	0.1	1	0.2	7.92	10
0.68	0.1	-	1	0.2	8.02	10
0.68	-	0.1	1	0.2	8.02	10
0.68	-	-	1	0.2	8.12	10

8/25

Electrophoresis

Notsu

Lane	sample/(µl)	Length(bp)
1	1kb ladder /3
2	[1] (inp xp cut) /10
3	[2] (inp x cut) /10
4	[3] (inp p cut) /10
5	[4] (inp no cut) /10

[img42]

8/26

Restriction Enzyme Digestion

Uchino, Notsu

DNA/(µl)	EcoR1/(µl)	Xba1/(µl)	BSA/(µl)	MilliQ/(µl)	Total/(µl)
8/24 miniprep INP 1c3 /0.68	-	1.0	0.2	7.12	10
8/15 miniprep RFP psb1c3 /1.3	-	1.0	0.2	6.5	10
8/24 miniprep INP 1c3 /0.68	0.1	1.0	0.2	7.02	10
8/15 miniprep RFP psb1c3 /1.3	0.1	1.0	0.2	6.4	10
8/24 miniprep INP 1c3/ 0.68	0.1	-	0.2	8.02	10
8/15 miniprep RFP psb1c3 /1.3	0.1	-	0.2	7.4	10
8/24 miniprep INP 1c3 /0.68	-	-	0.2	8.12	10
8/15 miniprep RFP psb1c3 /1.3	-	-	0.2	7.5	10

8/26

PCR (Target)

Li

Templates/(µl)	Primer1/(µl)	Primer2/(µl)	Buffer/(µl)	MilliQ/(µl)	Any Other/(µl)	Total/(µl)
scfv from MK	c.scfv fw/0.75	c.scfv rv/0.75	2x KAPA Hifi /12.5	11		25
none	c.scfv fw/0.75	c.scfv rv/0.75	2x KAPA Hifi /5	4.4		10

8/26

Activation Check of Xba

Uchino

Lane	sample/(µl)	Length(bp)
1	1kb ladder/2
2	[8] RFP/10
3	[6] RFP EcoRI/10
4	[2] RFP XbaI/10
5	[4] RFP EcoRI, XbaI /10
6	[7] INP /10
7	[5] INP EcoRI /10
8	[1] INP XbaI /10
9	[3] INP EcoRI, XbaI/10

[img45-1]
[img45-2]

8/27

Electrophoresis

Li

Lane	sample/(µl)	Length(bp)
2	1kb ladder	2
3	8/25 PCR scfv	5
4	8/25 PCR none	5

8/27

PCR (Target)

Li

Templates/(µl)	Primer1/(µl)	Primer2/(µl)	MilliQ/(µl)	Any Other/(µl)	Total/(µl)
s.BAN	BAN.Fw/ 0.75	BAN.Rv/ 0.75	11	2xKAPA HiFi/ 12.5	25
s.BAN	BAN.Fw/ 0.75	BAN.Rv/ 0.75	11	2xKAPA HiFi/ 12.5	25
s.BAN	BAN.Fw/ 0.75	BAN.Histag/ 0.75	11	2xKAPA HiFi/ 12.5	25
scfv	scfv.Fw/ 0.75	scfv.Rv/ 0.75	11	2xKAPA HiFi/ 12.5	25
8/24 miniprep INP	INP.Fw/ 0.75	INP.Rv/ 0.75	11	2xKAPA HiFi/ 12.5	25
8/24 miniprep INP	INP.Fw/ 0.75	INP.Rv.Histag/ 0.75	11	2xKAPA HiFi/ 12.5	25

8/27

PCR (Steps)

Li

Steps	Temparature	Time	Cycle
Predenature	95	3min	1
Denature	98	20sec	25
Annealing	65	15sec	25
Extension	72	30sec	25
Final Elogation	72	5min	1
Final Holding	4	-	1

9/8

Restriction Enzyme Digestion

Notsu

DNA/(µl)	EcoR1/(µl)	Spe1/(µl)	Pst1/(µl)	Buffer/(µl)	MilliQ/(µl)	Total/(µl)
[1] 8/15 miniprep 1c3 RFP /14	0.5	0.5	-	10xH /3	12	30
[2] 8/24 miniprep 3c5 RFP /8.5	0.5	-	0.5	10xH /3	17.5	30
[3] 8/24 miniprep 3c5 RFP /8.5	0.5	0.5	-	10xH /3	17.5	30
[4] 8/24 miniprep BBa_J61002 p- /24	0.5	-	0.5	10xH /3	2	30

9/9

Electrophoresis

Notsu

Lane	Restriction Enzyme Digestion Products
1	100bp ladder 9µL
2	8/24 miniprep p- EPcut 30µL

9/9

Electrophoresis

Sukegawa, Notsu

Lane	Restriction Enzyme Digestion Products
1	1kbp ladder 9µL
2	8/15 miniprep 1C3RFP EScut 30µL
3	8/24 miniprep 3C5RFP EPcut 30µL
4	8/24 miniprep 3C5RFP EScut 30µL

9/14

PCR (Target)

Li

Templates/(µl)	Primer1/(µl)	Primer2/(µl)	Buffer/(µl)	MilliQ/(µl)	Total/(µl)
8/24 miniprep INP	INPfw/0.75	INPRv/0.75	2×KAPAHiFi	11	25
8/24 miniprep INP	INPfw/0.75	INPHisRv/0.75	2×KAPAHiFi	11	25
BAN	BANFw/0/75	BANRv/0.75	2×KAPAHiFi	11	25
BAN	BANFw/0/75	BANHisRv/0.75	2×KAPAHiFi	11	25
BAN	BANFw/0/75	RBSRv/0.75	2×KAPAHiFi	11	25
scFv fromMk	scFvFw/0/75	scFvRv/0/75	2×KAPAHiFi	11	25
scFv fromMk	scFvHisFw/0.75	scFvRv/0.75	2×KAPAHiFi	11	25

9/14

PCR (Steps)

Li

Steps	Temparature	Time	Cycle
1	95	3min	１
2	98	70sec	25
3	68	15sec	25
4	72	30sec	25
5	72	5min	1
6	10	-	1

9/14

Gel Extraction

Kaneko

Lane	Restriction Enzyme Digestion Products
2	ladder/6
4	9/14 PCR1/10
6	9/14 PCR2/10
8	9/14 PCR3/10
10	9/14 PCR4/10
12	9/14PCR5/10
14	9/14 PCR6/10
16	9/14 PCR7/10

9/14

PCR (Target)

Li

Templates/(µl)	Primer1/(µl)	Primer2/(µl)	Buffer/(µl)	Total/(µl)
3sBAN/0.15+6scFvfromMK/0.15	BANFw/0/75	scFvRv/0.75	2×KAPAHiFi/12.5	25
4sBAN/0.15+7scFvfromMK/0.15	BANFw/0/75	scFvRv/0.75	2×KAPAHiFi/12.5	25
1INP8/24miniprep/0.15+5sBAN0.15+scFvfromMK/0.15	BANFw/0/75	scFvRv/0.75	2×KAPAHiFi/12.5	25
2INP8/24miniprep/0.15	BANFw/0/75	scFvRv/0.75	2×KAPAHiFi/12.5	25

9/15

Restriction Enzyme Digestion

Li, Uchino

Sample	DNA/(µl)	EcoR1/(µl)	Spe1/(µl)	Buffer/(µl)	Total/(µl)
3sBAN+6scFvfromMKPCRproduct	34	1	1	4	40
4sBAN+7scFvfromMK PCRproduct	34	1	1	4	40
1INP8/24miniprep+5sBAN+6scFvfromMKPCRproduct	16	1	1	2	20
2INP8/24miniprep+5sBAN+7scFvfromMkPCRproduct	34	1	1	4	40

9/15

Gel Extraction

Li, Uchino

Lane	Restriction Enzyme Digestion Product	Volume/(µl)
1	1kb ladder
2	3sBAN+6scFvfromMkPCRproductEScut	20
3	3sBAN+6scFvfromMkPCRproductEScut	20
4	4sBAN+7scFvfromMkPCRproductEScut	20
5	4sBAN+7scFvfromMkPCRproductEScut	20
6	1INP8/24miniprep+5sBAN+7scFvfromMkPCRproductEScut	20
7	2INP8/24miniprep+5sBAN+7scFvfromMkPCRproductEScut	20
8	2INP8/24miniprep+5sBAN+7scFvfromMkPCRproductEScut	20
9	RFP3C5PCRproduct

9/16

Colony PCR (2Column)

Uchino, Michimori

Name I	Name II
9/15 transformation BANscFv 3C5 colony1~16	VF+VR
9/15 transformation INPscFv 3C5 colony1~16	VF+VR
9/15 transformation BANHisscFv 3C5 colony1~16	VF+VR
9/15 transformation INPHisscFv 3C5 colony1~16	VF+VR

9/16

PCR (Steps)

Uchino, Michimori

Steps	Temparature	Time	Cycle
1	94	2	1
2	94	0.5	25
3	55	0.5	25
4	68	1	25
5	4	7	1
6	4	-	1

9/16

Gel Extraction

Li, Kaneko, Notsu

Lane	Restriction Enzyme Digestion Product	Volume/(µl)
1	1kb ladder	5
2	BANscFv3C5 colony 9	10
3	BANscFv3C5 colony 10	10
4	BANscFv3C5 colony 11	10
5	BANscFv3C5 colony 12	10
6	BANscFv3C5 colony 13	10
7	BANscFv3C5 colony 14	10
8	BANscFv3C5 colony 15	10
9	BANscFv3C5 colony 16	10
10	BANHisscFv3C5 colony 9	10
11	BANHisscFv3C5 colony 10	10
12	BANHisscFv3C5 colony 11	10
13	BANHisscFv3C5 colony 12	10
14	BANHisscFv3C5 colony 13	10
15	BANHisscFv3C5 colony 14	10
16	BANHisscFv3C5 colony 15	10
17	BANHisscFv3C5 colony 16	10
18	BANscFv3C5 colony 1	10

9/16

Gel Extraction

Li, Kaneko, Notsu

Lane	Restriction Enzyme Digestion Product	Volume/(µl)
1	1kb ladder	5
2	INPscFv3C5 colony 9	10
3	INPscFv3C5 colony 10	10
4	INPscFv3C5 colony 11	10
5	INPscFv3C5 colony 12	10
6	INPscFv3C5 colony 13	10
7	INPscFv3C5 colony 14	10
8	INPscFv3C5 colony 15	10
9	INPscFv3C5 colony 16	10
10	INPHisscFv3C5 colony 9	10
11	INPHisscFv3C5 colony 10	10
12	INPHisscFv3C5 colony 11	10
13	INPHisscFv3C5 colony 12	10
14	INPHisscFv3C5 colony 13	10
15	INPHisscFv3C5 colony 14	10
16	INPHisscFv3C5 colony 15	10
17	INPHisscFv3C5 colony 16	10
18	BANscFv3C5 colony 1	10

9/17

Electrophoresis

Michimori, Kaneko

Lane	Restriction Enzyme Digestion Products
1	buffer/5
2	1kb ladder/5
3	BAN 9 P/4.5
4	BAN 9 N/4.5
5	BAN14 P/4.5
6	BAN14 N/4.5
7	BANHis14 P/4.5
8	BANHis14/4.5

9/17

Electrophoresis

Kaneko

Lane	sample/(µl)	Length(bp)
1	1kbp ladder
2	none
3	BANHis16 p
4	BANHis16 n
5	INP 12 p
6	INP 14 p
7	INP 12 n
8	INP 14 n

9/17

Electrophoresis

Kaneko

Lane	Restriction Enzyme Digestion Products
1	-
2	-
3	1kbladder/5
4	INPHis9 P/4.5
5	INPHis9N/4.5
6	INPHis12P/4.5
7	INPHis12N/4.5
8	INP14 N/4.5

9/17

Restriction Enzyme Digestion

Uchino, Michimori

Sample	DNA/(µl)	EcoR1/(µl)	Pst1/(µl)	Buffer/(µl)	MilliQ/(µl)	Total/(µl)
BAN9	2	0.5	0.5	10×H/1	6	10
BAN9	1	0	0	10×H/1	8	10
BAN14	2	0.5	0.5	10×H/1	6	10
BAN14	1	0	0	10×H/1	8	10
BANHis14	2	0.5	0.5	10×H/1	6	10
BANHis 14	1	0	0	10×H/1	8	10
BANHis 16	2	0.5	0.5	10×H/1	6	10
BANHis16	1	0	0	10×H/1	8	10
INP12	2	0.5	0.5	10×H/1	6	10
INP12	1	0	0	10×H/1	8	10
INP14	2	0.5	0.5	10×H/1	6	10
INP 14	1	0	0	10×H/1	8	10
INP 9	2	0.5	0.5	10×H/1	6	10
INP9	1	0	0	10×H/1	8	10
INPHis12	2	0.5	0.5	10×H/1	6	10
INPHis12	1	0	0	10×H/1	8	10

9/17

Miniprep

Notsu

DNA	Concentration/(µg/ml)	260/280	260/230
9/16 liquid culture BANscFv12
9/16 liquid culture BANscFv14
9/16 liquid culture BANHisscFv12
9/16 liquid culture BANHisscFv14
9/16 liquid culture INPHisscFv12
9/16 liquid culture INPHisscFv14
9/16 liquid culture INPHisscFv10
9/16 liquid culture BANscFv

@@ Line 169: / Line 169: @@
 	    <div id="contents">
+<div class="kyoto-calendar">
+   <table>
+      <tr>
+         <th colspan=7>August</th>
+      </tr>
+      <tr>
+         <td></td>
+         <td></td>
+         <td></td>
+         <td></td>
+         <td></td>
+         <td></td>
+         <td>1</td>
+      </tr>
+      <tr>
+        <td>2</td>
+        <td>3</td>
+        <td>4</td>
+        <td>5</td>
+        <td>6</td>
+        <td>7</td>
+        <td>8</td>
+      </tr>
+      <tr>
+        <td>9</td>
+        <td>10</td>
+        <td>11</td>
+        <td>12</td>
+         <td>13</td>
+         <td><a href="#0814">14</a></td>
+         <td><a href="#0815">15</a></td>
+      </tr>
+      <tr>
+        <td><a href="#0816">16</a></td>
+        <td><a href="#0817">17</a></td>
+        <td><a href="#0818">18</a></td>
+        <td><a href="#0819">19</a></td>
+         <td><a href="#0820">20</a></td>
+         <td><a href="#0821">21</a></td>
+         <td>22</td>
+      </tr>
+      <tr>
+        <td>23</td>
+        <td><a href="#0824">24</a></td>
+        <td><a href="#0825">25</a></td>
+        <td><a href="#0826">26</a></td>
+         <td><a href="#0827">27</a></td>
+         <td>28</td>
+         <td>29</td>
+     </tr>
+      <tr>
+        <td>30</td>
+        <td>31</td>
+        <td></td>
+        <td></td>
+        <td></td>
+        <td></td>
+        <td></td>
+      </tr>
+   </table>
+   <table>
+      <tr>
+         <th colspan=7>September</th>
+      </tr>
+      <tr>
+         <td></td>
+         <td></td>
+         <td>1</td>
+         <td>2</td>
+         <td>3</td>
+         <td>4</td>
+         <td>5</td>
+      </tr>
+      <tr>
+         <td>6</td>
+         <td>7</td>
+         <td><a href="#0908">8</a></td>
+         <td><a href="#0909">9</a></td>
+         <td>10</td>
+         <td>11</td>
+         <td>12</td>
+      </tr>
+      <tr>
+         <td>13</td>
+         <td><a href="#0914">14</a></td>
+         <td><a href="#0915">15</a></td>
+         <td><a href="#0916">16</a></td>
+         <td><a href="#0917">17</a></td>
+         <td>18</td>
+         <td>19</td>
+      </tr>
+      <tr>
+         <td>20</td>
+         <td>21</td>
+         <td>22</td>
+         <td>23</td>
+         <td>24</td>
+         <td>25</td>
+         <td>26</td>
+      </tr>
+      <tr>
+         <td>27</td>
+         <td>28</td>
+         <td>29</td>
+         <td>30</td>
+         <td></td>
+         <td></td>
+         <td></td>
+      </tr>
+      <tr>
+         <td></td>
+         <td></td>
+         <td></td>
+         <td></td>
+      </tr>
+   </table>
+</div>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0814" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/14</h3></span>
+<span class="keln_exp"><h4>Transformation</h4></span>
+<span class="keln_researcher">Li, Uchino, Kaneko</span>
+<table class="keln_table">
+<tr><th>Sample Name</th><th>Sample Volume (&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Medium</th></tr>
+<tr><td>RFP 0.5ng/&micro;l</td><td>2</td><td>DH5α /20</td><td>LB(CP)</td></tr>
+<tr><td>RFP 5ng/&micro;l</td><td>2</td><td>DH5α /20</td><td>LB(CP)</td></tr>
+<tr><td>RFP 10ng/&micro;l</td><td>2</td><td>DH5α /20</td><td>LB(CP)</td></tr>
+<tr><td>RFP 20ng/&micro;l</td><td>2</td><td>DH5α /20</td><td>LB(CP)</td></tr>
+<tr><td>RFP 50ng/&micro;l</td><td>2</td><td>DH5α /20</td><td>LB(CP)</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0814" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/14</h3></span>
+<span class="keln_exp"><h4>Gel Extraction</h4></span>
+<span class="keln_researcher">Li, Uchino, Kaneko</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Product</th><th>Volume/(&micro;l)</th></tr>
+<tr><td>1</td><td>10/1 PQ(3)</td><td>about 23.5</td></tr>
+<tr><td>2</td><td>1kb ladder</td><td>6</td></tr>
+<tr><td>3</td><td>10/1 PQ(3)</td><td>about 23.5</td></tr>
+<tr><td>4</td><td>10/1 PQ(3)</td><td>about 24</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/7/7a/T--Kyoto--8_14_No1_Gel_Extraction_Notsu_09_19_12_14_09No.20814.jpg">
+</div>
+<p class="keln_note">Total volume of samples of lane 1~3 was 71.5&micro;l and it was divided into three.</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Egashira -->
+<div class="keln_container">
+<a name="0814" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/14</h3></span>
+<span class="keln_exp"><h4>Transformation</h4></span>
+<span class="keln_researcher">Li, Uchino, Kaneko</span>
+<table class="keln_table">
+<tr><th>Sample Name</th><th>Sample Volume</th><th>Competent Cells/(&micro;l)</th><th>Medium</th></tr>
+<tr><td>3C5</td><td>10&micro;l</td><td>DH5α 20&micro;l</td><td>LB(CP)</td></tr>
+<tr><td>P+</td><td>10&micro;l</td><td>DH5α 20&micro;l</td><td>LB(CP)</td></tr>
+<tr><td>P-</td><td>10&micro;l</td><td>DH5α 20&micro;l</td><td>LB(CP)</td></tr>
+<tr><td>RBS</td><td>10&micro;l</td><td>DH5α 20&micro;l</td><td>LB(CP)</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0814" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/14</h3></span>
+<span class="keln_exp"><h4>Liquid Culture</h4></span>
+<span class="keln_researcher">Li, Uchino, Iguchi, Kaneko</span>
+<table class="keln_table">
+<tr><th>Name</th><th>medium</th></tr>
+<tr><td>8/13 transformation RFP 20ng/&micro;l colony1</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony1</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony2</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony3</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony4</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony5</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony6</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony7</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony8</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony9</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony10</td><td>LB(CP)</td></tr>
+<tr><td>8/13 transformation part RFP colony11</td><td>LB(CP)</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0815" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/15</h3></span>
+<span class="keln_exp"><h4>Miniprep</h4></span>
+<span class="keln_researcher">Li, Uchino, Notsu, Kaneko, Watanabe</span>
+<table class="keln_table">
+<tr><th>DNA</th><th>Concentration/(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
+<tr><td>RFP1 /2&micro;l</td><td>82.5</td><td>1.40</td><td>1.22</td></tr>
+<tr><td>RFP2 /4&micro;l</td><td>142.5</td><td>1.69</td><td>2.05</td></tr>
+<tr><td>RFP3 /4&micro;l</td><td>50</td><td>1.55</td><td>1.71</td></tr>
+<tr><td>RFP4 /4&micro;l</td><td>57.5</td><td>1.64</td><td>1.88</td></tr>
+<tr><td>RFP6 /4&micro;l</td><td>98.75</td><td>1.71</td><td>1.40</td></tr>
+<tr><td>RFP(20) /4&micro;l</td><td>76.25</td><td>1.67</td><td>1.32</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Egashira -->
+<div class="keln_container">
+<a name="0815" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/15</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Notsu, Kaneko</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</th></tr>
+<tr><td>1</td><td>1kb ladder 3&micro;l</td></tr>
+<tr><td>2</td><td>RFP1 10&micro;l</td></tr>
+<tr><td>3</td><td>RFP2 10&micro;l</td></tr>
+<tr><td>4</td><td>RFP3 10&micro;l</td></tr>
+<tr><td>5</td><td>RFP4 10&micro;l</td></tr>
+<tr><td>6</td><td>RFP6 10&micro;l</td></tr>
+<tr><td>7</td><td>RFP(20) 10&micro;l</td></tr>
+<tr><td>8</td><td>PCR1 5&micro;l</td></tr>
+<tr><td>9</td><td>PCR2 5&micro;l</td></tr>
+<tr><td>10</td><td>PCR3 5&micro;l</td></tr>
+<tr><td>11</td><td>PCR4 5&micro;l</td></tr>
+<tr><td>12</td><td> PCR5 5 &micro;l</td></tr>
+<tr><td>13</td><td>1kb ladder 3&micro;l</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/5/5e/T--Kyoto--8_15_No6_Electrophoresis_Egashira_02_08_02_44_07.JPG">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Egashira -->
+<div class="keln_container">
+<a name="0815" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/15</h3></span>
+<span class="keln_exp"><h4>Restriction Enzyme Digestion</h4></span>
+<span class="keln_researcher">Uchino, Notsu, Kaneko</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>DNA/(&micro;l)</th><th>EcoR1/(&micro;l)</th><th>Xba1/(&micro;l)</th><th>Spe1/(&micro;l)</th><th>Pst1/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>BSA/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>8/15Miniprep PSB1C3-RFP1</td><td>24</td><td>1</td><td>0</td><td>0</td><td>1</td><td>5(H)</td><td>0</td><td>19</td><td>50</td></tr>
+<tr><td>8/15Miniprep PSB1C3-RFP2</td><td>14</td><td>1</td><td>0</td><td>0</td><td>1</td><td>5(H)</td><td>0</td><td>29</td><td>50</td></tr>
+<tr><td>8/15Miniprep PSB1C3-RFP3</td><td>40</td><td>1</td><td>0</td><td>0</td><td>1</td><td>5(H)</td><td>0</td><td>3</td><td>50</td></tr>
+<tr><td>8/15Miniprep PSB1C3-RFP4</td><td>35</td><td>1</td><td>0</td><td>0</td><td>1</td><td>5(H)</td><td>0</td><td>8</td><td>50</td></tr>
+<tr><td>8/15Miniprep PSB1C3-RFP6</td><td>20</td><td>1</td><td>0</td><td>0</td><td>1</td><td>5(H)</td><td>0</td><td>23</td><td>50</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0815" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/15</h3></span>
+<span class="keln_exp"><h4>colony PCR</h4></span>
+<span class="keln_researcher">Uchino, Notsu, Kaneko, Watanabe</span>
+<table class="keln_table">
+<tr><th>colony                                                                         Primers</th></tr>
+<tr><td>8/14 transformation pSB1C3-RFP1                     VF+VR</td></tr>
+<tr><td>8/14 transformation pSB1C3-RFP2                     VF+VR</td></tr>
+<tr><td>8/14 transformation pSB1C3-RFP3                     VF+VR</td></tr>
+<tr><td>8/14 transformation pSB1C3-RFP4                     VF+VR</td></tr>
+<tr><td>8/14 transformation pSB1C3-RFP1                     VF+VR</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0815" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/15</h3></span>
+<span class="keln_exp"><h4>PCR (Steps)</h4></span>
+<span class="keln_researcher">Uchino, Notsu, Kaneko, Watanabe</span>
+<table class="keln_table">
+<tr><th>Steps</th><th>Temparature</th><th>Time</th><th>Cycle</th></tr>
+<tr><td>Pre Denature</td><td>94℃</td><td>2min</td><td>1</td></tr>
+<tr><td>Denature</td><td>94℃</td><td>30sec</td><td>30</td></tr>
+<tr><td>Annealing</td><td>55℃</td><td>30sec</td><td>30</td></tr>
+<tr><td>Extension</td><td>68℃</td><td>1min</td><td>30</td></tr>
+<tr><td>Final elongation</td><td>4℃</td><td>7min</td><td>1</td></tr>
+<tr><td>Final Holding</td><td>4℃</td><td>-</td><td>-</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0816" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/16</h3></span>
+<span class="keln_exp"><h4>Gel Extraction</h4></span>
+<span class="keln_researcher">Li, Iguchi, Notsu, Kaneko, Watanabe</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Product</th><th>Volume/(&micro;l)</th></tr>
+<tr><td>1</td><td>1kb ladder</td><td>10</td></tr>
+<tr><td>2</td><td>blank</td><td></td></tr>
+<tr><td>3</td><td>8/15 digestion pSB1C3-RFP 1</td><td>55</td></tr>
+<tr><td>4</td><td>8/15 digestion pSB1C3-RFP 2</td><td>55</td></tr>
+<tr><td>5</td><td>8/15 digestion pSB1C3-RFP 3</td><td>36</td></tr>
+<tr><td>6</td><td>8/15 digestion pSB1C3-RFP 4</td><td>36</td></tr>
+<tr><td>7</td><td>8/15 digestion pSB1C3-RFP 6</td><td>36</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/7/77/T--Kyoto--8_16_No10_Gel_Extraction_Notsu_09_26_01_32_24.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0816" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/16</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Li, Iguchi, Notsu, Kaneko, Watanabe</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>8/16 Gel Extraction RFP1 /1</td><td></td></tr>
+<tr><td>2</td><td>8/16 Gel Extraction RFP2 /1</td><td></td></tr>
+<tr><td>3</td><td>8/16 Gel Extraction RFP3 /1</td><td></td></tr>
+<tr><td>4</td><td>8/16 Gel Extraction RFP4 /1</td><td></td></tr>
+<tr><td>5</td><td>blank</td><td></td></tr>
+<tr><td>6</td><td>8/16 pSB1C3-vector /1</td><td></td></tr>
+<tr><td>7</td><td>8/16 pSB1C3-vector /1</td><td></td></tr>
+<tr><td>8</td><td>1kladder</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/6/6f/T--Kyoto--8_16_No11_Electrophoresis_Notsu_09_26_01_55_23.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0816" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/16</h3></span>
+<span class="keln_exp"><h4>Ligation</h4></span>
+<span class="keln_researcher">Li, Iguchi, Notsu, Kaneko, Watanabe</span>
+<table class="keln_table">
+<tr><th>Vector DNA /(&micro;l)</th><th>insert DNA /(&micro;l)</th><th>ligase /(&micro;l)</th><th>10×buffer /(&micro;l)</th><th>MilliQ /(&micro;l)</th><th>Total /(&micro;l)</th></tr>
+<tr><td>pSB1C3 EPcut /1</td><td>RFP /4</td><td>0.5</td><td>1</td><td>3</td><td>9.5</td></tr>
+<tr><td>pSB1C3 EPcut /1</td><td>RFP /2</td><td>0.5</td><td>1</td><td>5</td><td>9.5</td></tr>
+<tr><td>pSB1C3 EPcut /1</td><td>RFP /4</td><td>0.5</td><td>1</td><td>3</td><td>9.5</td></tr>
+<tr><td>pSB1C3 EPcut /1</td><td>RFP /4</td><td>0.5</td><td>1</td><td>3</td><td>9.5</td></tr>
+<tr><td>pSB1C3 EPcut /1</td><td>-</td><td>0.5</td><td>1</td><td>7</td><td>9.5</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0817" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/17</h3></span>
+<span class="keln_exp"><h4>Liquid Culture</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Name</th><th>medium</th></tr>
+<tr><td>8/16 transformation pSB3C5 RFP1</td><td>LB(CP) /6</td></tr>
+<tr><td>8/16 transformation pSB3C5 RFP2</td><td>LB(CP) /6</td></tr>
+<tr><td>8/16 transformation pSB3C5 RFP1</td><td>LB(CP) /6</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0817" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/17</h3></span>
+<span class="keln_exp"><h4>Transformation</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Sample Name</th><th>Sample Volume (&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Medium</th></tr>
+<tr><td>pSB1C3 P+</td><td>2</td><td>DH5α /20</td><td>LB</td></tr>
+<tr><td>pSB1C3 P-</td><td>2</td><td>DH5α /20</td><td>LB</td></tr>
+<tr><td>pSB1C3 RBS</td><td>2</td><td>DH5α /20</td><td>LB</td></tr>
+<tr><td>pSB3C5 RFP</td><td>2</td><td>DH5α /20</td><td>LB</td></tr>
+<tr><td>8/16 ligation pSB1C3 RFP1</td><td>2</td><td>DH5α</td><td>LB</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Notsu -->
+<div class="keln_container">
+<a name="0817" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/17</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Iguchi</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kbp ladder /3</td><td></td></tr>
+<tr><td>2</td><td>blank</td><td></td></tr>
+<tr><td>3</td><td>pSB1C3-RFP(ligate)1 /2</td><td></td></tr>
+<tr><td>4</td><td>pSB1C3-RFP(ligate)2 /2</td><td></td></tr>
+<tr><td>5</td><td>pSB1C3-RFP(ligate)3 /2</td><td></td></tr>
+<tr><td>6</td><td>pSB1C3-RFP(ligate)4 /3</td><td></td></tr>
+<tr><td>7</td><td>Vector (Ctrl) /5</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/c/c7/T--Kyoto--8_17_No14_Electrophoresis_Notsu_09_29_12_26_11.JPG">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0818" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/18</h3></span>
+<span class="keln_exp"><h4>Liquid Culture</h4></span>
+<span class="keln_researcher">Li, Iguchi, Notsu</span>
+<table class="keln_table">
+<tr><th>Name</th><th>medium</th></tr>
+<tr><td>INP [1] BBa_K811005</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>INP [2] BBa_K811005</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>INP [3] BBa_K811005</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>scfv04 [1] BBa_K875004</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>scfv04 [2] BBa_K875004</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>scfv04 [3] BBa_K875004</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>scfv06 [1] BBa__K875006</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>scfv06 [2] BBa_K875006</td><td>LB(CP) / 6ml</td></tr>
+<tr><td>scfv06 [3] BBa_K875006</td><td>LB(CP) / 6ml</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0818" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/18</h3></span>
+<span class="keln_exp"><h4>Miniprep</h4></span>
+<span class="keln_researcher">Li, Iguchi, Notsu</span>
+<table class="keln_table">
+<tr><th>DNA</th><th>Concentration/(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
+<tr><td>8/17 Liquid Culture 3C5 [1] / 5&micro;l</td><td>77</td><td>1.69</td><td>1.45</td></tr>
+<tr><td>8/17 Liquid Culture 3C5 [2] / 2&micro;l</td><td>40</td><td>1.67</td><td>1.17</td></tr>
+<tr><td>8/17 Liquid Culture 3C5 [3] / 2&micro;l</td><td>15</td><td>3.75</td><td>3.70</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0818" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/18</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder /1</td><td>-</td></tr>
+<tr><td>2</td><td>[1] psb3c5(8/18 miniprep) /4.2</td><td>3807</td></tr>
+<tr><td>3</td><td>[2] psb3c5(8/18 miniprep) /4.2</td><td>3807</td></tr>
+<tr><td>4</td><td>[1'] psb3c5(8/18 miniprep) /4.2</td><td>3807</td></tr>
+<tr><td>5</td><td>[2'] psb3c5(8/18 miniprep) /4.2</td><td>3807</td></tr>
+<tr><td>6</td><td>[3'] psb3c5(8/18 miniprep) /4.2</td><td>3807</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/c/c5/T--Kyoto--8_18_No18_Electrophoresis_Kaneko_05_20_04_12_53.jpg">
+</div>
+<p class="keln_note">[img18]</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0818" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/18</h3></span>
+<span class="keln_exp"><h4>Restriction Enzyme Digestion</h4></span>
+<span class="keln_researcher">Li, Iguchi, Notsu</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>DNA/(&micro;l)</th><th>EcoR1/(&micro;l)</th><th>Xba1/(&micro;l)</th><th>Spe1/(&micro;l)</th><th>Pst1/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>BSA/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td></td><td>psb3c5(8/18miniprep) [1'], [2'], [3'] mixed / 50</td><td>1.0</td><td></td><td></td><td>1.0</td><td>5.6</td><td></td><td></td><td>56.6</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0819" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/19</h3></span>
+<span class="keln_exp"><h4>Transformation</h4></span>
+<span class="keln_researcher">Li, Iguchi, Notsu</span>
+<table class="keln_table">
+<tr><th>Sample Name</th><th>Sample Volume (&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Medium</th></tr>
+<tr><td>BBa_ J61002 p+</td><td>2</td><td>DH5a /20</td><td>LB(Amp)</td></tr>
+<tr><td>BBa_J61002 p-</td><td>2</td><td>DH5a /20</td><td>LB(Amp)</td></tr>
+<tr><td>BBa_1A2 RBS</td><td>2</td><td>DH5a /20</td><td>LB(Amp)</td></tr>
+</table>
+</div>
+<p class="keln_note">Failed due to deactivation of Amp</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0819" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/19</h3></span>
+<span class="keln_exp"><h4>MutationPCR</h4></span>
+<span class="keln_researcher"></span>
+<table class="keln_table">
+<tr><th>Templates /&micro;l</th><th>Primer /&micro;l</th><th>Primer STAR Max Premix /&micro;l</th><th>MilliQ /&micro;l</th><th>Total(&micro;l)</th></tr>
+<tr><td>scfv plasmid /0.1</td><td>m.scfv FW /1, m.scfvRV/1</td><td>25</td><td>22.9</td><td>50</td></tr>
+<tr><td>scfv plasmid (nega) /0.1</td><td>0</td><td>5</td><td>4.9</td><td>10</td></tr>
+<tr><td>(nega) /0</td><td>m.scfv FW/0.2, m.scfvRV/0.2</td><td>5</td><td>4.6</td><td>10</td></tr>
+</table>
+</div>
+<p class="keln_note">---30cycle---<br>98℃ 10''<br>55℃ 15''<br>72℃ 20''<br>----------------<br>4℃ holding</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0819" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/19</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Notsu</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder/2</td><td>-</td></tr>
+<tr><td>2</td><td>scfv plasmid +primer(m.scfv FW, m.scfv RV) mutation PCR product /5</td><td>3600</td></tr>
+<tr><td>3</td><td>scfv plasmid mutation PCR product /5</td><td>-</td></tr>
+<tr><td>4</td><td>primer(m.scfv FW, m.scfv RV) mutation PCR product /5</td><td>-</td></tr>
+<tr><td>5</td><td>psb3c5 EPcut product /5</td><td>2600(backborn), 1200(inseert<RFP>)</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/6/64/T--Kyoto--8_19_No22_Electrophoresis_Kaneko_05_20_04_27_06.jpg">
+</div>
+<p class="keln_note">[img22]</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0819" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/19</h3></span>
+<span class="keln_exp"><h4>Concentration</h4></span>
+<span class="keln_researcher">Li, Notsu</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>OD600</th><th>Bacterial cells /ml</th></tr>
+<tr><td>INP [1] K811005</td><td>0.643</td><td>5.14x10^8</td></tr>
+<tr><td>scfv06 K875006</td><td>0.634</td><td>5.07x10^8</td></tr>
+<tr><td>scfv04 K875004</td><td>0.618</td><td>4.94x10^8</td></tr>
+</table>
+</div>
+<p class="keln_note">1 OD 600 equals to 8x10^8 cells /ml</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0819" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/19</h3></span>
+<span class="keln_exp"><h4>Miniprep</h4></span>
+<span class="keln_researcher">Li, Notsu</span>
+<table class="keln_table">
+<tr><th>DNA</th><th>Concentration/(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
+<tr><td>scfv 04[1] K875004 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>scfv 04[2] K875004 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>scfv 04[3] K875004 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>scfv 06[1] K875006 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>scfv 06[2] K875006 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>scfv 06[3] K875006 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>INP [1] K811005 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>INP [2] K811005 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+<tr><td>INP [3] K811005 /3&micro;l</td><td>56</td><td>1.63</td><td>1.45</td></tr>
+</table>
+</div>
+<p class="keln_note">There was a problem with an instrument</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0820" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/20</h3></span>
+<span class="keln_exp"><h4>Transformation</h4></span>
+<span class="keln_researcher">Li, Uchino</span>
+<table class="keln_table">
+<tr><th>Sample Name</th><th>Sample Volume (&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Medium</th></tr>
+<tr><td>8/19 mutation PCR scfv plasmid (nega)</td><td>1</td><td>DH5a/ 20</td><td>LB(CP)</td></tr>
+</table>
+</div>
+<p class="keln_note">8/19 mutation PCR:<br>1&micro;l extraction of scfv plasmid (nega)/10&micro;l was folded 300 times.</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0820" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/20</h3></span>
+<span class="keln_exp"><h4>Transformation</h4></span>
+<span class="keln_researcher">Li, Uchino</span>
+<table class="keln_table">
+<tr><th>Sample Name</th><th>Sample Volume (&micro;l)</th><th>Competent Cells/(&micro;l)</th><th>Medium</th></tr>
+<tr><td>8/19 mutation PCR scfv plasmid</td><td>1 [note:1]</td><td>DH5a/20</td><td>LB(AMP)</td></tr>
+<tr><td>8/19 mutation PCR scfv plasmid (nega)</td><td>1 [note:2]</td><td>DH5a/20</td><td>LB(AMP)</td></tr>
+<tr><td>BBa_J61002 p+</td><td>1</td><td>DH5a/20</td><td>LB(AMP)</td></tr>
+<tr><td>BBa_J61002 p-</td><td>1</td><td>DH5a/20</td><td>LB(AMP)</td></tr>
+<tr><td>BBa_1A2 RBS</td><td>1</td><td>DH5a/20</td><td>LB(AMP)</td></tr>
+</table>
+</div>
+<p class="keln_note">[note:1]<br>8/19 mutation PCR:<br>1&micro;l extraction of scfv plasmid (nega)/50&micro;l was folded 300 times.<br>[note:2]<br>8/19 mutation PCR:<br>1&micro;l extraction of scfv plasmid (nega)/10&micro;l was folded 300 times.</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0820" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/20</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder /1.5</td><td></td></tr>
+<tr><td>2</td><td>9/19 miniprep scfv04 [1]</td><td></td></tr>
+<tr><td>3</td><td>9/19 miniprep scfv04 [2]</td><td></td></tr>
+<tr><td>4</td><td>9/19 miniprep scfv04 [3]</td><td></td></tr>
+<tr><td>5</td><td>9/19 miniprep scfv06 [1]</td><td></td></tr>
+<tr><td>6</td><td>9/19 miniprep scfv06 [2]</td><td></td></tr>
+<tr><td>7</td><td>9/19 miniprep scfv06 [3]</td><td></td></tr>
+<tr><td>8</td><td>9/19 miniprep INP [1]</td><td></td></tr>
+<tr><td>9</td><td>9/19 miniprep INP [2]</td><td></td></tr>
+<tr><td>10</td><td>9/19 miniprep INP [3]</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/5/53/T--Kyoto--8_20_No26_Electrophoresis_Kaneko_05_20_04_44_45.jpg">
+</div>
+<p class="keln_note">[img26]</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>Colony PCR</h4></span>
+<span class="keln_researcher">Uchino</span>
+<table class="keln_table">
+<tr><th>Colony</th><th>Primers</th></tr>
+<tr><td>8/20 transformation RBS</td><td>VF+VR</td></tr>
+<tr><td>8/20 transformation p+</td><td>VF+VR</td></tr>
+<tr><td>8/20 transformation p-</td><td>VF+VR</td></tr>
+<tr><td>8/20 dilution INP</td><td>VF+VR</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>PCR (Steps)</h4></span>
+<span class="keln_researcher">Uchino</span>
+<table class="keln_table">
+<tr><th>Steps</th><th>Temparature</th><th>Time</th><th>Cycle</th></tr>
+<tr><td>Pre</td><td>94℃</td><td>2min</td><td>1</td></tr>
+<tr><td>De</td><td>94℃</td><td>30 sec</td><td>30</td></tr>
+<tr><td>An</td><td>55℃</td><td>30 sec</td><td>30</td></tr>
+<tr><td>Ex</td><td>68℃</td><td>1min</td><td>30</td></tr>
+<tr><td>Final E</td><td>4℃</td><td>7min</td><td>1</td></tr>
+<tr><td>Final Hold</td><td>4℃</td><td>-</td><td>-</td></tr>
+</table>
+</div>
+<p class="keln_note">Autoclaved distrilled water /23&micro;l<br>2xQuick taq /25&micro;l<br>10 pmol/&micro;l primer (VF) /1&micro;l<br>10 pmol/&micro;l primer (VR) /1&micro;l<br>Template DNA /0&micro;l<br>Total 50&micro;l</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder /2</td><td></td></tr>
+<tr><td>2</td><td>8/21 colony PCR RBS /all</td><td></td></tr>
+<tr><td>3</td><td>8/21 colony PCR p+ /all</td><td></td></tr>
+<tr><td>4</td><td>8/21 colony PCR p- /all</td><td></td></tr>
+<tr><td>5</td><td>8/21 colony PCR INP /all</td><td></td></tr>
+<tr><td>6</td><td>8/21 colony PCR Nega /all</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/0/04/T--Kyoto--8_21_No28_Electrophoresis_Kaneko_05_20_04_54_01%2C1.jpg"><img src="https://static.igem.org/mediawiki/2016/b/b5/T--Kyoto--8_21_No28_Electrophoresis_Kaneko_05_20_04_54_01%2C2.jpg">
+</div>
+<p class="keln_note">[img29-1]<br>weak band except INP -> retry<br>colony PCR and Electrophoresis were conducted under the same condition.<br>[img29-2]<br>RBS and INP success.<br>P+ and P-  failed  -> retry.</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>PCR (Steps)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Steps</th><th>Temparature</th><th>Time</th><th>Cycle</th></tr>
+<tr><td>Predenature</td><td>95℃</td><td>3min</td><td>1</td></tr>
+<tr><td>Denature</td><td>98℃</td><td>20sec</td><td>25</td></tr>
+<tr><td>Annealing</td><td>70℃</td><td>15sec</td><td>25</td></tr>
+<tr><td>Extension</td><td>72℃</td><td>30sec</td><td>25</td></tr>
+<tr><td>Final Elongation</td><td>72℃</td><td>5min</td><td>1</td></tr>
+<tr><td>Final Holding</td><td>4℃</td><td>-</td><td>1</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>PCR (Target)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Templates/(&micro;l)</th><th>Primer1/(&micro;l)</th><th>Primer2/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Any Other/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>scfv 8/20 transformation</td><td>cscfv.f /0.75</td><td>cscfv.r /0.75</td><td>2x KAPA Hifi /12.5</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>- (nega)</td><td>cscfv.f /0.3</td><td>cscfv.f /0.3</td><td>2x KAPA Hifi /5</td><td>4.4</td><td></td><td>10</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>Colony PCR</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Colony</th><th>Primers</th></tr>
+<tr><td>8/20 transformation p+ [1]</td><td>VF+VR</td></tr>
+<tr><td>8/20 transformation p+ [2]</td><td>VF+VR</td></tr>
+<tr><td>8/20 transformation p+ [3]</td><td>VF+VR</td></tr>
+<tr><td>8/20 transformation p- [1]</td><td>VF+VR</td></tr>
+<tr><td>8/20 transformation p- [2]</td><td>VF+VR</td></tr>
+<tr><td>8/20 transformation p- [3]</td><td>VF+VR</td></tr>
+<tr><td>8/18 parts p+</td><td>VF+VR</td></tr>
+<tr><td>8/18 parts p-</td><td>VF+VR</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>PCR (Steps)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Steps</th><th>Temparature</th><th>Time</th><th>Cycle</th></tr>
+<tr><td>pre</td><td>94℃</td><td>2min</td><td>1</td></tr>
+<tr><td>de</td><td>94℃</td><td>30sec</td><td>30</td></tr>
+<tr><td>an</td><td>55℃</td><td>30sec</td><td>30</td></tr>
+<tr><td>ex</td><td>68℃</td><td>1min</td><td>30</td></tr>
+<tr><td>F.elong</td><td>4℃</td><td>7min</td><td>1</td></tr>
+<tr><td>F.hold</td><td>4℃</td><td>-</td><td>1</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>Liquid Culture</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Name</th><th>medium</th></tr>
+<tr><td>strain</td><td>Medium/ml</td></tr>
+<tr><td>8/20 transformation RBS</td><td>PG(AMP) /6</td></tr>
+<tr><td>8/20 dilution INP7</td><td>PG(CP) /6</td></tr>
+<tr><td>8/17 transformation 3c5, RFP</td><td>PG(CP) /6</td></tr>
+<tr><td>8/20 transformation p+ [1]</td><td>PG(AMP) /6</td></tr>
+<tr><td>8/20 transformation p- [1]</td><td>PG(AMP) /6</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0821" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/21</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder /1.5</td><td></td></tr>
+<tr><td>2</td><td>8/21 cloning PCR scfv[1] /2</td><td></td></tr>
+<tr><td>3</td><td>8/21 cloning PCR scfv[2] /2</td><td></td></tr>
+<tr><td>4</td><td>nega /2</td><td></td></tr>
+<tr><td>5</td><td>8/21 colony PCR p+ [1] /2</td><td></td></tr>
+<tr><td>6</td><td>8/21 colony PCR p+ [2] /2</td><td></td></tr>
+<tr><td>7</td><td>8/21 colony PCR p+ [3] /2</td><td></td></tr>
+<tr><td>8</td><td>8/21 colony PCR p- [1] /2</td><td></td></tr>
+<tr><td>9</td><td>8/21 colony PCR p- [2] /2</td><td></td></tr>
+<tr><td>10</td><td>8/21 colony PCR p- [3] /2</td><td></td></tr>
+<tr><td>11</td><td>8/21 colony PCR parts p+ /2</td><td></td></tr>
+<tr><td>12</td><td>8/21 colony PCR parts p- /2</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/9/9d/T--Kyoto--8_21_No34_Electrophoresis_Kaneko_05_20_05_13_01.jpg">
+</div>
+<p class="keln_note">[img35]<br>2, 3, 4 cloning PCR failed.<br>1 forgot to put ladder<br>5, 7, 8, 9 strong band<br>6 weak<br>10 failed<br>P+[1] and p-[1] -> liquid culture</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0824" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/24</h3></span>
+<span class="keln_exp"><h4>Ligation</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>sample/&micro;l</th><th>MilliQ (&micro;l)</th><th>Buffer /&micro;l</th><th>Ligase(&micro;l)</th><th>Total(&micro;l)</th></tr>
+<tr><td>8/19 mutation PCR scfv /5</td><td>3.5</td><td>1</td><td>0.5</td><td>10</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0824" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/24</h3></span>
+<span class="keln_exp"><h4>Liquid Culture</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Name</th><th>medium</th></tr>
+<tr><td>8/20 transformation m.scfv colonyA /6</td><td>PG(AMP)</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0824" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/24</h3></span>
+<span class="keln_exp"><h4>Miniprep</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>DNA</th><th>Concentration/(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
+<tr><td>8/20 Liquid culture RBS</td><td>124.5</td><td>1.65</td><td>1.02</td></tr>
+<tr><td>8/20 Liquid culture INP</td><td>147.8</td><td>1.66</td><td>0.98</td></tr>
+<tr><td>8/20 Liquid culture 3c5.RFP</td><td>234.8</td><td>1.79</td><td>1.62</td></tr>
+<tr><td>8/20 Liquid culture P+[1]</td><td>40.3</td><td>1.78</td><td>1.60</td></tr>
+<tr><td>8/20 Liquid culture P-[1]</td><td>56.7</td><td>1.80</td><td>2.05</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Iguchi -->
+<div class="keln_container">
+<a name="0825" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/25</h3></span>
+<span class="keln_exp"><h4>Miniprep</h4></span>
+<span class="keln_researcher">Li, Uchino, Notsu, Kaneko</span>
+<table class="keln_table">
+<tr><th>DNA</th><th>Concentration/(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
+<tr><td>8/24 LIquid Culture m.scfv colonyA</td><td>-</td><td>-</td><td>-</td></tr>
+<tr><td>8/24 LIquid Culture m.scfv colonyB</td><td>-</td><td>-</td><td>-</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0825" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/25</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher"></span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder /1.5</td><td></td></tr>
+<tr><td>2</td><td>8/24 miniprep p+(J61002) /2</td><td></td></tr>
+<tr><td>3</td><td>8/24 miniprep p-(J61002) /2</td><td></td></tr>
+<tr><td>4</td><td>8/24 miniprep RFP (3c5) /2</td><td></td></tr>
+<tr><td>5</td><td>8/24 miniprep INP (1c3) /2</td><td></td></tr>
+<tr><td>6</td><td>8/24 miniprep RBS (1A2) /2</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/f/fd/T--Kyoto--8_25_No38_Electrophoresis_Kaneko_05_20_05_22_35.jpg">
+</div>
+<p class="keln_note">[img38]</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0825" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/25</h3></span>
+<span class="keln_exp"><h4>Restriction Enzyme Digestion</h4></span>
+<span class="keln_researcher">Uchino</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>DNA/(&micro;l)</th><th>EcoR1/(&micro;l)</th><th>Xba1/(&micro;l)</th><th>Spe1/(&micro;l)</th><th>Pst1/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>BSA/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td></td><td>p+/24.8</td><td></td><td></td><td>1</td><td>1</td><td>10xH /4</td><td></td><td>9.2</td><td>40</td></tr>
+<tr><td></td><td>IINP /6.8</td><td></td><td>1</td><td></td><td>1</td><td>10xM /4</td><td></td><td>27.2</td><td>40</td></tr>
+<tr><td></td><td>p- /17.6</td><td></td><td></td><td>1</td><td>1</td><td>10xH /4</td><td></td><td>16.4</td><td>40</td></tr>
+<tr><td></td><td>RBS /8.0</td><td></td><td></td><td>1</td><td>1</td><td>10xH /4</td><td></td><td>26</td><td>40</td></tr>
+<tr><td></td><td>RFP /4.3</td><td>1</td><td></td><td></td><td>1</td><td>10xH /4</td><td></td><td>29.7</td><td>40</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0825" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/25</h3></span>
+<span class="keln_exp"><h4>Gel Extraction</h4></span>
+<span class="keln_researcher">Uchino, Notsu</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Product</th><th>Volume/(&micro;l)</th></tr>
+<tr><td>1</td><td>1kb ladder </td><td>6</td></tr>
+<tr><td>2, 3</td><td>8/25 digestion p+</td><td>24 each</td></tr>
+<tr><td>4, 5</td><td>8/25 digestion p- </td><td>24 each</td></tr>
+<tr><td>6, 7</td><td>8/25 digestion RFP </td><td>24 each</td></tr>
+<tr><td>8, 9</td><td>8/25 digestion INP </td><td>24 each</td></tr>
+<tr><td>10, 11</td><td>8/25 digestion RBS </td><td>24 each</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/7/77/T--Kyoto--8_25_No40_Gel_Extraction_Kaneko_05_20_05_28_41.JPG">
+</div>
+<p class="keln_note">[img40]</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0825" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/25</h3></span>
+<span class="keln_exp"><h4>Actvation check of Xba and Pst</h4></span>
+<span class="keln_researcher">Uchino</span>
+<table class="keln_table">
+<tr><th>INP(&micro;l)</th><th>Xba(&micro;l)</th><th>Pst(&micro;l)</th><th>10xM(&micro;l)</th><th>BSA(&micro;l)</th><th>MilliQ(&micro;l)</th><th>Total(&micro;l)</th></tr>
+<tr><td>0.68</td><td>0.1</td><td>0.1</td><td>1</td><td>0.2</td><td>7.92</td><td>10</td></tr>
+<tr><td>0.68</td><td>0.1</td><td>-</td><td>1</td><td>0.2</td><td>8.02</td><td>10</td></tr>
+<tr><td>0.68</td><td>-</td><td>0.1</td><td>1</td><td>0.2</td><td>8.02</td><td>10</td></tr>
+<tr><td>0.68</td><td>-</td><td>-</td><td>1</td><td>0.2</td><td>8.12</td><td>10</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0825" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/25</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Notsu</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder /3</td><td></td></tr>
+<tr><td>2</td><td>[1] (inp xp cut) /10</td><td></td></tr>
+<tr><td>3</td><td>[2] (inp x cut) /10</td><td></td></tr>
+<tr><td>4</td><td>[3] (inp p cut) /10</td><td></td></tr>
+<tr><td>5</td><td>[4] (inp no cut) /10</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/4/46/T--Kyoto--8_25_No41_2_Electrophoresis_Kaneko_05_20_05_34_47.jpg">
+</div>
+<p class="keln_note">[img42]</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0826" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/26</h3></span>
+<span class="keln_exp"><h4>Restriction Enzyme Digestion</h4></span>
+<span class="keln_researcher">Uchino, Notsu</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>DNA/(&micro;l)</th><th>EcoR1/(&micro;l)</th><th>Xba1/(&micro;l)</th><th>Spe1/(&micro;l)</th><th>Pst1/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>BSA/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td></td><td>8/24 miniprep INP 1c3 /0.68</td><td>-</td><td>1.0</td><td></td><td></td><td></td><td>0.2</td><td>7.12</td><td>10</td></tr>
+<tr><td></td><td>8/15 miniprep RFP psb1c3 /1.3</td><td>-</td><td>1.0</td><td></td><td></td><td></td><td>0.2</td><td>6.5</td><td>10</td></tr>
+<tr><td></td><td>8/24 miniprep INP 1c3 /0.68</td><td>0.1</td><td>1.0</td><td></td><td></td><td></td><td>0.2</td><td>7.02</td><td>10</td></tr>
+<tr><td></td><td>8/15 miniprep RFP psb1c3 /1.3</td><td>0.1</td><td>1.0</td><td></td><td></td><td></td><td>0.2</td><td>6.4</td><td>10</td></tr>
+<tr><td></td><td>8/24 miniprep INP 1c3/ 0.68</td><td>0.1</td><td>-</td><td></td><td></td><td></td><td>0.2</td><td>8.02</td><td>10</td></tr>
+<tr><td></td><td>8/15 miniprep RFP psb1c3 /1.3</td><td>0.1</td><td>-</td><td></td><td></td><td></td><td>0.2</td><td>7.4</td><td>10</td></tr>
+<tr><td></td><td>8/24 miniprep INP 1c3 /0.68</td><td>-</td><td>-</td><td></td><td></td><td></td><td>0.2</td><td>8.12</td><td>10</td></tr>
+<tr><td></td><td>8/15 miniprep RFP psb1c3 /1.3</td><td>-</td><td>-</td><td></td><td></td><td></td><td>0.2</td><td>7.5</td><td>10</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0826" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/26</h3></span>
+<span class="keln_exp"><h4>PCR (Target)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Templates/(&micro;l)</th><th>Primer1/(&micro;l)</th><th>Primer2/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Any Other/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>scfv from MK</td><td>c.scfv fw/0.75</td><td>c.scfv rv/0.75</td><td>2x KAPA Hifi /12.5</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>none</td><td>c.scfv fw/0.75</td><td>c.scfv rv/0.75</td><td>2x KAPA Hifi /5</td><td>4.4</td><td></td><td>10</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0826" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/26</h3></span>
+<span class="keln_exp"><h4>Activation Check of Xba</h4></span>
+<span class="keln_researcher">Uchino</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kb ladder/2</td><td></td></tr>
+<tr><td>2</td><td>[8] RFP/10</td><td></td></tr>
+<tr><td>3</td><td>[6] RFP EcoRI/10</td><td></td></tr>
+<tr><td>4</td><td>[2] RFP XbaI/10</td><td></td></tr>
+<tr><td>5</td><td>[4] RFP EcoRI, XbaI /10</td><td></td></tr>
+<tr><td>6</td><td>[7] INP /10</td><td></td></tr>
+<tr><td>7</td><td>[5] INP EcoRI /10</td><td></td></tr>
+<tr><td>8</td><td>[1] INP XbaI /10</td><td></td></tr>
+<tr><td>9</td><td>[3] INP EcoRI, XbaI/10</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/1/17/T--Kyoto--8_26_No45_Electrophoresis_Kaneko_05_20_05_42_54.jpg">
+</div>
+<p class="keln_note">[img45-1]<br>[img45-2]</p>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Iguchi -->
+<div class="keln_container">
+<a name="0827" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/27</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>2</td><td>1kb ladder</td><td>2</td></tr>
+<tr><td>3</td><td>8/25 PCR scfv</td><td>5</td></tr>
+<tr><td>4</td><td>8/25 PCR none</td><td>5</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/f/fc/T--Kyoto--8_27_No46_Electrophoresis_Iguchi_10_17_06_08_50.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Iguchi -->
+<div class="keln_container">
+<a name="0827" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/27</h3></span>
+<span class="keln_exp"><h4>PCR (Target)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Templates/(&micro;l)</th><th>Primer1/(&micro;l)</th><th>Primer2/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Any Other/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>s.BAN</td><td>BAN.Fw/ 0.75</td><td>BAN.Rv/ 0.75</td><td></td><td>11</td><td>2xKAPA HiFi/ 12.5</td><td>25</td></tr>
+<tr><td>s.BAN</td><td>BAN.Fw/ 0.75</td><td>BAN.Rv/ 0.75</td><td></td><td>11</td><td>2xKAPA HiFi/ 12.5</td><td>25</td></tr>
+<tr><td>s.BAN</td><td>BAN.Fw/ 0.75</td><td>BAN.Histag/ 0.75</td><td></td><td>11</td><td>2xKAPA HiFi/ 12.5</td><td>25</td></tr>
+<tr><td>scfv</td><td>scfv.Fw/ 0.75</td><td>scfv.Rv/ 0.75</td><td></td><td>11</td><td>2xKAPA HiFi/ 12.5</td><td>25</td></tr>
+<tr><td>8/24 miniprep INP</td><td>INP.Fw/ 0.75</td><td>INP.Rv/ 0.75</td><td></td><td>11</td><td>2xKAPA HiFi/ 12.5</td><td>25</td></tr>
+<tr><td>8/24 miniprep INP</td><td>INP.Fw/ 0.75</td><td>INP.Rv.Histag/ 0.75</td><td></td><td>11</td><td>2xKAPA HiFi/ 12.5</td><td>25</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Iguchi -->
+<div class="keln_container">
+<a name="0827" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>8/27</h3></span>
+<span class="keln_exp"><h4>PCR (Steps)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Steps</th><th>Temparature</th><th>Time</th><th>Cycle</th></tr>
+<tr><td>Predenature</td><td>95</td><td>3min</td><td>1</td></tr>
+<tr><td>Denature</td><td>98</td><td>20sec</td><td>25</td></tr>
+<tr><td>Annealing</td><td>65</td><td>15sec</td><td>25</td></tr>
+<tr><td>Extension</td><td>72</td><td>30sec</td><td>25</td></tr>
+<tr><td>Final Elogation</td><td>72</td><td>5min</td><td>1</td></tr>
+<tr><td>Final Holding</td><td>4</td><td>-</td><td>1</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Kaneko -->
+<div class="keln_container">
+<a name="0908" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/8</h3></span>
+<span class="keln_exp"><h4>Restriction Enzyme Digestion</h4></span>
+<span class="keln_researcher">Notsu</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>DNA/(&micro;l)</th><th>EcoR1/(&micro;l)</th><th>Xba1/(&micro;l)</th><th>Spe1/(&micro;l)</th><th>Pst1/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>BSA/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td></td><td>[1] 8/15 miniprep 1c3 RFP /14</td><td>0.5</td><td></td><td>0.5</td><td>-</td><td>10xH /3</td><td></td><td>12</td><td>30</td></tr>
+<tr><td></td><td>[2] 8/24 miniprep 3c5 RFP /8.5</td><td>0.5</td><td></td><td>-</td><td>0.5</td><td>10xH /3</td><td></td><td>17.5</td><td>30</td></tr>
+<tr><td></td><td>[3] 8/24 miniprep 3c5 RFP /8.5</td><td>0.5</td><td></td><td>0.5</td><td>-</td><td>10xH /3</td><td></td><td>17.5</td><td>30</td></tr>
+<tr><td></td><td>[4] 8/24 miniprep BBa_J61002 p- /24</td><td>0.5</td><td></td><td>-</td><td>0.5</td><td>10xH /3</td><td></td><td>2</td><td>30</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/4/4c/T--Kyoto--9_8_No50_Restriction_Enzyme_Digestion_Kaneko_05_20_05_48_28.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto,Iguchi -->
+<div class="keln_container">
+<a name="0909" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/9</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Notsu</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</th></tr>
+<tr><td>1</td><td>100bp ladder 9&micro;L</td></tr>
+<tr><td>2</td><td>8/24 miniprep p- EPcut 30&micro;L</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto,Iguchi -->
+<div class="keln_container">
+<a name="0909" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/9</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Sukegawa, Notsu</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</th></tr>
+<tr><td>1</td><td>1kbp ladder 9&micro;L</td></tr>
+<tr><td>2</td><td>8/15 miniprep 1C3RFP EScut 30&micro;L</td></tr>
+<tr><td>3</td><td>8/24 miniprep 3C5RFP EPcut 30&micro;L</td></tr>
+<tr><td>4</td><td>8/24 miniprep 3C5RFP EScut 30&micro;L</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0914" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/14</h3></span>
+<span class="keln_exp"><h4>PCR (Target)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Templates/(&micro;l)</th><th>Primer1/(&micro;l)</th><th>Primer2/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Any Other/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>8/24 miniprep INP</td><td>INPfw/0.75</td><td>INPRv/0.75</td><td>2×KAPAHiFi</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>8/24 miniprep INP</td><td>INPfw/0.75</td><td>INPHisRv/0.75</td><td>2×KAPAHiFi</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>BAN</td><td>BANFw/0/75</td><td>BANRv/0.75</td><td>2×KAPAHiFi</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>BAN</td><td>BANFw/0/75</td><td>BANHisRv/0.75</td><td>2×KAPAHiFi</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>BAN </td><td>BANFw/0/75</td><td>RBSRv/0.75</td><td>2×KAPAHiFi</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>scFv fromMk</td><td>scFvFw/0/75</td><td>scFvRv/0/75</td><td>2×KAPAHiFi</td><td>11</td><td></td><td>25</td></tr>
+<tr><td>scFv fromMk</td><td>scFvHisFw/0.75</td><td>scFvRv/0.75</td><td>2×KAPAHiFi</td><td>11</td><td></td><td>25</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0914" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/14</h3></span>
+<span class="keln_exp"><h4>PCR (Steps)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Steps</th><th>Temparature</th><th>Time</th><th>Cycle</th></tr>
+<tr><td>1</td><td>95</td><td>3min</td><td>１</td></tr>
+<tr><td>2</td><td>98</td><td>70sec</td><td>25</td></tr>
+<tr><td>3</td><td>68</td><td>15sec</td><td>25</td></tr>
+<tr><td>4</td><td>72</td><td>30sec</td><td>25</td></tr>
+<tr><td>5</td><td>72</td><td>5min</td><td>1</td></tr>
+<tr><td>6</td><td>10</td><td>-</td><td>1</td></tr>
+</table>
+</div>
+<!------------ Table END ------------><!-- Table Generated by KELN Author: Yamamoto,Iguchi -->
+<div class="keln_container">
+<a name="0914" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/14</h3></span>
+<span class="keln_exp"><h4>Gel Extraction</h4></span>
+<span class="keln_researcher">Kaneko</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</th></tr>
+<tr><td>2</td><td>ladder/6</td></tr>
+<tr><td>4</td><td>9/14 PCR1/10</td></tr>
+<tr><td>6</td><td>9/14 PCR2/10</td></tr>
+<tr><td>8</td><td>9/14 PCR3/10</td></tr>
+<tr><td>10</td><td>9/14 PCR4/10</td></tr>
+<tr><td>12</td><td>9/14PCR5/10</td></tr>
+<tr><td>14</td><td>9/14 PCR6/10</td></tr>
+<tr><td>16</td><td>9/14 PCR7/10</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/4/4b/T--Kyoto--9_14_No53_Electrophoresis_Yamamoto_08_23_11_27_01.1.JPG"><img src="https://static.igem.org/mediawiki/2016/7/77/T--Kyoto--9_14_No53_Electrophoresis_Yamamoto_08_23_11_27_01.2.JPG">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0914" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/14</h3></span>
+<span class="keln_exp"><h4>PCR (Target)</h4></span>
+<span class="keln_researcher">Li</span>
+<table class="keln_table">
+<tr><th>Templates/(&micro;l)</th><th>Primer1/(&micro;l)</th><th>Primer2/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Any Other/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>3sBAN/0.15+6scFvfromMK/0.15</td><td>BANFw/0/75</td><td>scFvRv/0.75</td><td>2×KAPAHiFi/12.5</td><td></td><td></td><td>25</td></tr>
+<tr><td>4sBAN/0.15+7scFvfromMK/0.15</td><td>BANFw/0/75</td><td>scFvRv/0.75</td><td>2×KAPAHiFi/12.5</td><td></td><td></td><td>25</td></tr>
+<tr><td>1INP8/24miniprep/0.15+5sBAN0.15+scFvfromMK/0.15</td><td>BANFw/0/75</td><td>scFvRv/0.75</td><td>2×KAPAHiFi/12.5</td><td></td><td></td><td>25</td></tr>
+<tr><td>2INP8/24miniprep/0.15</td><td>BANFw/0/75</td><td>scFvRv/0.75</td><td>2×KAPAHiFi/12.5</td><td></td><td></td><td>25</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto,Iguchi -->
+<div class="keln_container">
+<a name="0914" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/15</h3></span>
+<span class="keln_exp"><h4>Restriction Enzyme Digestion</h4></span>
+<span class="keln_researcher">Li, Uchino</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>DNA/(&micro;l)</th><th>EcoR1/(&micro;l)</th><th>Xba1/(&micro;l)</th><th>Spe1/(&micro;l)</th><th>Pst1/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>BSA/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>3sBAN+6scFvfromMKPCRproduct</td><td>34</td><td>1</td><td></td><td>1</td><td></td><td>4</td><td></td><td></td><td>40</td></tr>
+<tr><td>4sBAN+7scFvfromMK PCRproduct</td><td>34</td><td>1</td><td></td><td>1</td><td></td><td>4</td><td></td><td></td><td>40</td></tr>
+<tr><td>1INP8/24miniprep+5sBAN+6scFvfromMKPCRproduct</td><td>16</td><td>1</td><td></td><td>1</td><td></td><td>2</td><td></td><td></td><td>20</td></tr>
+<tr><td>2INP8/24miniprep+5sBAN+7scFvfromMkPCRproduct</td><td>34</td><td>1</td><td></td><td>1</td><td></td><td>4</td><td></td><td></td><td>40</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0915" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/15</h3></span>
+<span class="keln_exp"><h4>Gel Extraction</h4></span>
+<span class="keln_researcher">Li, Uchino</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Product</th><th>Volume/(&micro;l)</th></tr>
+<tr><td>1</td><td>1kb ladder</td><td></td></tr>
+<tr><td>2</td><td>3sBAN+6scFvfromMkPCRproductEScut</td><td>20</td></tr>
+<tr><td>3</td><td>3sBAN+6scFvfromMkPCRproductEScut</td><td>20</td></tr>
+<tr><td>4</td><td>4sBAN+7scFvfromMkPCRproductEScut</td><td>20</td></tr>
+<tr><td>5</td><td>4sBAN+7scFvfromMkPCRproductEScut</td><td>20</td></tr>
+<tr><td>6</td><td>1INP8/24miniprep+5sBAN+7scFvfromMkPCRproductEScut</td><td>20</td></tr>
+<tr><td>7</td><td>2INP8/24miniprep+5sBAN+7scFvfromMkPCRproductEScut</td><td>20</td></tr>
+<tr><td>8</td><td>2INP8/24miniprep+5sBAN+7scFvfromMkPCRproductEScut</td><td>20</td></tr>
+<tr><td>9</td><td>RFP3C5PCRproduct</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/a/a2/T--Kyoto--9_15_No56_Gel_Extraction_Yamamoto_08_23_11_46_18%2C1.jpg"><img src="https://static.igem.org/mediawiki/2016/5/52/T--Kyoto--9_15_No56_Gel_Extraction_Yamamoto_08_23_11_46_18%2C2.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0916" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/16</h3></span>
+<span class="keln_exp"><h4>Colony PCR (2Column)</h4></span>
+<span class="keln_researcher">Uchino, Michimori</span>
+<table class="keln_table">
+<tr><th>Name I</th><th>Name II</th></tr>
+<tr><td>9/15 transformation BANscFv 3C5 colony1~16</td><td>VF+VR</td></tr>
+<tr><td>9/15 transformation INPscFv 3C5 colony1~16</td><td>VF+VR</td></tr>
+<tr><td>9/15 transformation BANHisscFv 3C5 colony1~16</td><td>VF+VR</td></tr>
+<tr><td>9/15 transformation INPHisscFv 3C5 colony1~16</td><td>VF+VR</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0916" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/16</h3></span>
+<span class="keln_exp"><h4>PCR (Steps)</h4></span>
+<span class="keln_researcher">Uchino, Michimori</span>
+<table class="keln_table">
+<tr><th>Steps</th><th>Temparature</th><th>Time</th><th>Cycle</th></tr>
+<tr><td>1</td><td>94</td><td>2</td><td>1</td></tr>
+<tr><td>2</td><td>94</td><td>0.5</td><td>25</td></tr>
+<tr><td>3</td><td>55</td><td>0.5</td><td>25</td></tr>
+<tr><td>4</td><td>68</td><td>1</td><td>25</td></tr>
+<tr><td>5</td><td>4</td><td>7</td><td>1</td></tr>
+<tr><td>6</td><td>4</td><td>-</td><td>1</td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0916" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/16</h3></span>
+<span class="keln_exp"><h4>Gel Extraction</h4></span>
+<span class="keln_researcher">Li, Kaneko, Notsu</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Product</th><th>Volume/(&micro;l)</th></tr>
+<tr><td>1</td><td>1kb ladder</td><td>5</td></tr>
+<tr><td>2</td><td>BANscFv3C5 colony 9</td><td>10</td></tr>
+<tr><td>3</td><td>BANscFv3C5 colony 10</td><td>10</td></tr>
+<tr><td>4</td><td>BANscFv3C5 colony 11</td><td>10</td></tr>
+<tr><td>5</td><td>BANscFv3C5 colony 12</td><td>10</td></tr>
+<tr><td>6</td><td>BANscFv3C5 colony 13</td><td>10</td></tr>
+<tr><td>7</td><td>BANscFv3C5 colony 14</td><td>10</td></tr>
+<tr><td>8</td><td>BANscFv3C5 colony 15</td><td>10</td></tr>
+<tr><td>9</td><td>BANscFv3C5 colony 16</td><td>10</td></tr>
+<tr><td>10</td><td>BANHisscFv3C5 colony 9</td><td>10</td></tr>
+<tr><td>11</td><td>BANHisscFv3C5 colony 10</td><td>10</td></tr>
+<tr><td>12</td><td>BANHisscFv3C5 colony 11</td><td>10</td></tr>
+<tr><td>13</td><td>BANHisscFv3C5 colony 12</td><td>10</td></tr>
+<tr><td>14</td><td>BANHisscFv3C5 colony 13</td><td>10</td></tr>
+<tr><td>15</td><td>BANHisscFv3C5 colony 14</td><td>10</td></tr>
+<tr><td>16</td><td>BANHisscFv3C5 colony 15</td><td>10</td></tr>
+<tr><td>17</td><td>BANHisscFv3C5 colony 16</td><td>10</td></tr>
+<tr><td>18</td><td>BANscFv3C5 colony 1</td><td>10</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/d/d3/T--Kyoto--9_16_No59_Gel_Extraction_Yamamoto_08_23_11_57_19.1.JPG"><img src="https://static.igem.org/mediawiki/2016/3/34/T--Kyoto--9_16_No59_Gel_Extraction_Yamamoto_08_23_11_57_19.2.JPG"><img src="https://static.igem.org/mediawiki/2016/a/aa/T--Kyoto--9_16_No59_Gel_Extraction_Yamamoto_08_23_11_57_19.3.JPG"><img src="https://static.igem.org/mediawiki/2016/6/67/T--Kyoto--9_16_No59_Gel_Extraction_Yamamoto_08_23_11_57_19.4.JPG">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0916" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/16</h3></span>
+<span class="keln_exp"><h4>Gel Extraction</h4></span>
+<span class="keln_researcher">Li, Kaneko, Notsu</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Product</th><th>Volume/(&micro;l)</th></tr>
+<tr><td>1</td><td>1kb ladder</td><td>5</td></tr>
+<tr><td>2</td><td>INPscFv3C5 colony 9</td><td>10</td></tr>
+<tr><td>3</td><td>INPscFv3C5 colony 10</td><td>10</td></tr>
+<tr><td>4</td><td>INPscFv3C5 colony 11</td><td>10</td></tr>
+<tr><td>5</td><td>INPscFv3C5 colony 12</td><td>10</td></tr>
+<tr><td>6</td><td>INPscFv3C5 colony 13</td><td>10</td></tr>
+<tr><td>7</td><td>INPscFv3C5 colony 14</td><td>10</td></tr>
+<tr><td>8</td><td>INPscFv3C5 colony 15</td><td>10</td></tr>
+<tr><td>9</td><td>INPscFv3C5 colony 16</td><td>10</td></tr>
+<tr><td>10</td><td>INPHisscFv3C5 colony 9</td><td>10</td></tr>
+<tr><td>11</td><td>INPHisscFv3C5 colony 10</td><td>10</td></tr>
+<tr><td>12</td><td>INPHisscFv3C5 colony 11</td><td>10</td></tr>
+<tr><td>13</td><td>INPHisscFv3C5 colony 12</td><td>10</td></tr>
+<tr><td>14</td><td>INPHisscFv3C5 colony 13</td><td>10</td></tr>
+<tr><td>15</td><td>INPHisscFv3C5 colony 14</td><td>10</td></tr>
+<tr><td>16</td><td>INPHisscFv3C5 colony 15</td><td>10</td></tr>
+<tr><td>17</td><td>INPHisscFv3C5 colony 16</td><td>10</td></tr>
+<tr><td>18</td><td>BANscFv3C5 colony 1</td><td>10</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/3/3d/T--Kyoto--9_16_No60_Gel_Extraction_Yamamoto_08_23_11_59_27%2C1.jpg"><img src="https://static.igem.org/mediawiki/2016/8/88/T--Kyoto--9_16_No60_Gel_Extraction_Yamamoto_08_23_11_59_27%2C2.jpg"><img src=""><img src="https://static.igem.org/mediawiki/2016/4/42/T--Kyoto--9_16_No60_Gel_Extraction_Yamamoto_08_23_11_59_27%2C3.jpg"><img src="https://static.igem.org/mediawiki/2016/6/69/T--Kyoto--9_16_No60_Gel_Extraction_Yamamoto_08_23_11_59_27%2C4.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto,Iguchi -->
+<div class="keln_container">
+<a name="0916" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/17</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Michimori, Kaneko</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</th></tr>
+<tr><td>1</td><td>buffer/5</td></tr>
+<tr><td>2</td><td>1kb ladder/5</td></tr>
+<tr><td>3</td><td>BAN 9 P/4.5</td></tr>
+<tr><td>4</td><td>BAN 9 N/4.5</td></tr>
+<tr><td>5</td><td>BAN14 P/4.5</td></tr>
+<tr><td>6</td><td>BAN14 N/4.5</td></tr>
+<tr><td>7</td><td>BANHis14 P/4.5</td></tr>
+<tr><td>8</td><td>BANHis14/4.5</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/9/98/T--Kyoto--9_17_No62_Electrophoresis_Yamamoto_08_23_12_42_58.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Iguchi -->
+<div class="keln_container">
+<a name="0917" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/17</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Kaneko</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>sample/(&micro;l)</th><th>Length(bp)</th></tr>
+<tr><td>1</td><td>1kbp ladder</td><td></td></tr>
+<tr><td>2</td><td>none</td><td></td></tr>
+<tr><td>3</td><td>BANHis16 p</td><td></td></tr>
+<tr><td>4</td><td>BANHis16 n</td><td></td></tr>
+<tr><td>5</td><td>INP 12 p</td><td></td></tr>
+<tr><td>6</td><td>INP 14 p</td><td></td></tr>
+<tr><td>7</td><td>INP 12 n</td><td></td></tr>
+<tr><td>8</td><td>INP 14 n</td><td></td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/9/9b/T--Kyoto--9_17_No63_Electrophoresis_Iguchi_10_17_06_39_35.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto,Iguchi -->
+<div class="keln_container">
+<a name="0917" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/17</h3></span>
+<span class="keln_exp"><h4>Electrophoresis</h4></span>
+<span class="keln_researcher">Kaneko</span>
+<table class="keln_table">
+<tr><th>Lane</th><th>Restriction Enzyme Digestion Products</th></tr>
+<tr><td>1</td><td>-</td></tr>
+<tr><td>2</td><td>-</td></tr>
+<tr><td>3</td><td>1kbladder/5</td></tr>
+<tr><td>4</td><td>INPHis9 P/4.5</td></tr>
+<tr><td>5</td><td>INPHis9N/4.5</td></tr>
+<tr><td>6</td><td>INPHis12P/4.5</td></tr>
+<tr><td>7</td><td>INPHis12N/4.5</td></tr>
+<tr><td>8</td><td>INP14 N/4.5</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/b/b8/T--Kyoto--9_17_No64_Electrophoresis_Yamamoto_08_23_01_05_58.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0917" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/17</h3></span>
+<span class="keln_exp"><h4>Restriction Enzyme Digestion</h4></span>
+<span class="keln_researcher">Uchino, Michimori</span>
+<table class="keln_table">
+<tr><th>Sample</th><th>DNA/(&micro;l)</th><th>EcoR1/(&micro;l)</th><th>Xba1/(&micro;l)</th><th>Spe1/(&micro;l)</th><th>Pst1/(&micro;l)</th><th>Buffer/(&micro;l)</th><th>BSA/(&micro;l)</th><th>MilliQ/(&micro;l)</th><th>Total/(&micro;l)</th></tr>
+<tr><td>BAN9 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>BAN9 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+<tr><td>BAN14 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>BAN14 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+<tr><td>BANHis14 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>BANHis 14 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+<tr><td>BANHis 16 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>BANHis16 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+<tr><td>INP12 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>INP12 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+<tr><td>INP14 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>INP 14 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+<tr><td>INP 9 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>INP9 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+<tr><td>INPHis12 </td><td>2</td><td>0.5</td><td></td><td></td><td>0.5</td><td>10×H/1</td><td></td><td>6</td><td>10</td></tr>
+<tr><td>INPHis12 </td><td>1</td><td>0</td><td></td><td></td><td>0</td><td>10×H/1</td><td></td><td>8</td><td>10</td></tr>
+</table>
+<img src="https://static.igem.org/mediawiki/2016/8/8a/T--Kyoto--9_17_No65_Restriction_Enzyme_Digestion_Yamamoto_08_23_01_13_52.jpg">
+</div>
+<!------------ Table END ------------>
+<!-- Table Generated by KELN Author: Yamamoto -->
+<div class="keln_container">
+<a name="0917" class = "kyoto-jump"></a>
+<span class="keln_date"><h3>9/17</h3></span>
+<span class="keln_exp"><h4>Miniprep</h4></span>
+<span class="keln_researcher">Notsu</span>
+<table class="keln_table">
+<tr><th>DNA</th><th>Concentration/(&micro;g/ml)</th><th>260/280</th><th>260/230</th></tr>
+<tr><td>9/16 liquid culture BANscFv12</td><td></td><td></td><td></td></tr>
+<tr><td>9/16 liquid culture BANscFv14</td><td></td><td></td><td></td></tr>
+<tr><td>9/16 liquid culture BANHisscFv12</td><td></td><td></td><td></td></tr>
+<tr><td>9/16 liquid culture BANHisscFv14</td><td></td><td></td><td></td></tr>
+<tr><td>9/16 liquid culture INPHisscFv12</td><td></td><td></td><td></td></tr>
+<tr><td>9/16 liquid culture INPHisscFv14</td><td></td><td></td><td></td></tr>
+<tr><td>9/16 liquid culture INPHisscFv10</td><td></td><td></td><td></td></tr>
+<tr><td>9/16 liquid culture BANscFv</td><td></td><td></td><td></td></tr>
+</table>
+</div>
+<!------------ Table END ------------>
      </div>