Difference between revisions of "Team:Tsinghua/Safety"

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           <a href="https://2016.igem.org/Team:Tsinghua/Description">Description</a><br>
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           <a href="https://2016.igem.org/Team:Tsinghua/Team">Team members</a><br>
 
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           <a href="https://2016.igem.org/Team:Tsinghua/Experiments">Design</a><br>
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           <a href="https://2016.igem.org/Team:Tsinghua/Proof">Proof of concepts</a><br>
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          <a href="https://2016.igem.org/Team:Tsinghua/Safety" >Safety</a><br>
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          <h1 class="contentPage" id="tag_safety">Safety</h1><br>
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          <h1 class="contentPage" id="tag_teamMembers">team members</h1>
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          <h2 class="contentPage">Overview</h2>
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            <h2 class="contentPage">We are family</h2>
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          This year, Team Tsinghua proposed a CRISPR/Cas9-based gene surveillance system and
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          <br> attempted to realize such design in baking yeasts. Even though we have deliberately
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          <br> chosen E. coli and S. cerevisiae as chassis organisms, of which biosafety levels are
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          <br>well recognized and the protocol for experimental manipulation is well established, there
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          <br> could have been unsolicited consequences if no attention for safe experiments is heeded.
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          <br>Therefore, in order to minimize any unwanted accident, we have received proper training
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          <br>and adopted several safety regulations in the laboratory as follows.
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          <h2 class="contentPage">Lab safety training</h2>
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          First, before we started our project, all of our team members had received strict lab
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          <br> safety training, including standard experimental protocols, proper disposal of biological
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          <br> and chemical waste, information on hazardous chemicals and use of biosafety cabinets etc
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          <br>from two senior members Liu Yan and Qin Yiran in Prof. Dai’s laboratory. Such guidance were
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          <br> documented on the online portal in detail. Team members involved in wet works all went through
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          <br> orientations with mentors or managers specifying proper equipment usage and safety before
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          <br>gaining access to that lab.
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          <h2 class="contentPage">Appropriate protection</h2>
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          Second, risks to team members brought about by potentially hazardous chemicals were minimized
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          <br> by wearing appropriate personal protective equipments (PPEs) throughout lab time and
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          <br> following lab safety regulations of Tsinghua University. Gloves, lab coats and close-toed
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          <br> shoes are required when working with chassis organisms. Hazardous chemicals such as ethidium
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          <br> bromide (EB) were carefully used and disposed according to protocols. No food or drink is
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          <br> allowed in the working area.
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          <h2 class="contentPage">chassis organism management</h2>
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          Third, E. coli strains that are used by our project (DH5α and BL21) as well as the yeast strain
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          <br> are harmless and commonly accepted as safe organisms to work with. Our modified chassis
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          <br> organism will be properly used and disposed according to the safety manual of the lab and
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          <br> will not be released to the natural environment, a crucial principal of Prof Dai’s laboratory.
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          <br> We amplified all plasmids to be used in yeast-related experiments in E. coli.
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          <h2 class="contentPage">Safety in future</h2>
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          Fourth, in terms of future application, we also have a clear vision: we want to test our system
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          <br> in yeasts as a proof-of-concept, and if such system is sensitive and safe enough, we want
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            <strong style="margin-left: 210px;">Mao&nbsp;Tianyang</strong>
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          <br> to advance its application in mammalian cell lines. In the future, such system can be applied
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            <strong style="margin-left: 295px;">Huang&nbsp;Yiming</strong>
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          <br> in gene therapy to treat diseases like cancer with the help of viral vectors. Since we want to
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          <br> combine the viral vector with our surveillance system, any potential risk that a viral vector
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          <br> may bring in is worth consideration with scrutiny, such as unwanted genomic insertion of viral
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              The brightest star in the sky<strong style="margin-left: 185px;"></strong>The brightest star in the sky
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          <br> vectors. Therefore, in order to achieve our goal, we will first test the efficacy and safety level
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          <br> of the viral vector used in cell lines and then in mice. Also, we hope to introduce a well-controlled
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          <br> molecular switch that only allows for bacterial and yeast growth in an artificial setting, therefore
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          <br> to prevent any accidental release of the organisms we use.
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            <strong style="margin-left: 220px;">Wu&nbsp;Bohou</strong>
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              The brightest star in the sky<strong style="margin-left: 185px;"></strong>The brightest star in the sky
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            <strong style="margin-left: 213px;">Cui&nbsp;Xiaoyue</strong>
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            <strong style="margin-left: 213px;">Luo&nbsp;Mingjie</strong>
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                  <span>2016 iGEM Tsinghua - All rights reserved</span>
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                  <span>Adapted from Bootstrap and FIVB</span>
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Revision as of 21:59, 19 October 2016

Team

team members

We are family





Mao Tianyang Huang Yiming
The brightest star in the skyThe brightest star in the sky





Wu Bohou Zou Xinzhi
The brightest star in the skyThe brightest star in the sky





Lu Yao Jia Zeran
The brightest star in the skyThe brightest star in the sky





Jin Qiao Ge Chenghao
The brightest star in the skyThe brightest star in the sky





Zhao Yiqing Xiao Yangbo
The brightest star in the skyThe brightest star in the sky





Cui Xiaoyue Yang Sichen
The brightest star in the skyThe brightest star in the sky





Luo Mingjie Yan Jun
The brightest star in the skyThe brightest star in the sky





2016 iGEM Tsinghua - All rights reserved Adapted from Bootstrap and FIVB Follow us: igem2016_tsinghua@126.com