Difference between revisions of "Team:Valencia UPV/HP/Silver"

Line 61: Line 61:
 
</head>
 
</head>
 
   <div id="mainContent">
 
   <div id="mainContent">
−
 
 
−
  <div class="row">
 
−
    <div class="col-xs-12">
 
−
      <button type="button" onclick="location.href='https://2016.igem.org/Team:Valencia_UPV/Protocols'">ALISIA PROTOCOLOS POR AQUÍ</button>
 
−
    </div>
 
−
  </div>
 
 
    
 
    
 
   <div class="row">
 
   <div class="row">

Revision as of 14:18, 1 August 2016

Home

May 18, 2016

Take glycerinated cultures of C58 Agrobacterium with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.

May 19, 2016

Refresh previously made culture by inoculating 10 μL in a new culture medium.

May 20, 2016

Agroinfiltration in Nicotiana benthamiana of C58 with dsRED.

June 6, 2016

  • Take from the glycerinates of Goldenbraid Collection:

    Plasmid GB Code
    pD6B3 α1 GB0015
    pD6B3 α2 GB0017
    pD6B3 Ω1 GB0019
    pD6B3 Ω2 GB0021
    pUPD2 GB0307

  • Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37°C overnight.

  • Experiment with snails:

    • Let both N. benthamianaleafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.