Difference between revisions of "Team:UCLA/Notebook"

Line 65: Line 65:
 
         <h5>Isolation of template DNA</h5>
 
         <h5>Isolation of template DNA</h5>
 
         <p> Miniprep of EC93 and EC869 </p>
 
         <p> Miniprep of EC93 and EC869 </p>
 +
        <ul>
 +
          <li>EC93 #1: 145.42 ng/μL</li>
 +
          <li>EC93 #2: 198.85 ng/μL</li>
 +
          <li>EC869 #1: 90.81 ng/μL</li>
 +
          <li>EC869 #2: 45.95 ng/μL</li>
 +
        </ul>
 +
        <p> gDNA isolation of ATCC 13048 </p>
 +
        <ul>
 +
        <li> 34.87 ng/μL </li>
 +
        </ul>
 +
        <p> Gibson primers designed for EBL1 and EBL2 toxin switched constructs in pSB1C3 and pSC101</p>
 +
      </div>
 +
    </div>
 +
 +
  <div class="row day-entry">
 +
      <div class="columns small-2 small-offset-1 day-date">
 +
        <span>Jun</span>
 +
        <p>29</p>
 +
      </div>
 +
      <div class="columns small-9">
 +
        <h5>Isolation of template DNA</h5>
 +
        <p> <b> Miniprep of EC93 and EC869 </b> </p>
 
         <ul>  
 
         <ul>  
 
           <li>EC93 #1: 145.42 ng/μL</li>
 
           <li>EC93 #1: 145.42 ng/μL</li>

Revision as of 22:51, 9 August 2016

JOURNAL

Projects

CDI

Protein Cages

Week

1

Construction of ATCC 13048 Loci 2 in pSB1C3

Isolation of template DNA from EC93, EC869, and ATCC 13048; PCR of Gibson fragments for EBL2 in pSB1C3 construct

Jun

27

Inoculation of strains obtained from Dr. Christopher Hayes

Inoculated overnight cultures of:

  • EPI100 w/ EC93 cosmid
  • X90 w/ EC869 cosmid
  • ATCC 13048

Conditions:

  • 10mL LB
  • 10uL ampicillin
  • 37 C for 16h shaking
Jun

28

Isolation of template DNA

Miniprep of EC93 and EC869

  • EC93 #1: 145.42 ng/μL
  • EC93 #2: 198.85 ng/μL
  • EC869 #1: 90.81 ng/μL
  • EC869 #2: 45.95 ng/μL

gDNA isolation of ATCC 13048

  • 34.87 ng/μL

Gibson primers designed for EBL1 and EBL2 toxin switched constructs in pSB1C3 and pSC101

Jun

29

Isolation of template DNA

Miniprep of EC93 and EC869

  • EC93 #1: 145.42 ng/μL
  • EC93 #2: 198.85 ng/μL
  • EC869 #1: 90.81 ng/μL
  • EC869 #2: 45.95 ng/μL

gDNA isolation of ATCC 13048

  • 34.87 ng/μL

Gibson primers designed for EBL1 and EBL2 toxin switched constructs in pSB1C3 and pSC101

Week

1

Title For The Week

Description blah blah blah

Jun

24

Title for the day (e.g. "Cloning Mutants")

Today we did cloning. fuck cloning. blah blah blah.