Team:Valencia UPV/Notebook

18May

    Take glycerinated cultures of C58 Agrobacterium with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28^C.

    19May

      Refresh previously made culture by inoculating 10 μL in a new culture medium.

      20May

        Agroinfiltration in Nicotiana benthamiana of C58 with dsRED. href

        06Jun

          • Take from the glycerinates of Goldenbraid Collection:

          PlasmidGB Code
          pD6B3 α1GB0015
          pD6B3 α2GB0017
          pD6B3 Ω1GB0019
          pD6B3 Ω2GB0021
          pUPD2GB0307

          Prepare liquid culture (3 mL LB + 3 μL antibiotic) 1:1000. Incubate at 37^C overnight.

          • Experiment with snails:

            • Two experiments: one with infiltrated Nicotiana benthamiana and another with not infiltrated N.benthamiana (negative control). Lettuce leafs haven’t been correctly infiltrated and it seems that the expression level is low.
            • Let both N. benthamiana leafs with snails overnight at room temperature in separated boxes. We will observe if snails eat the leafs and if appears fluorescence.


          Sponsors