Our project could not have existed without the following people, and we are eternally grateful for their contributions.
“TOOLBOX” FORMULATION
University of Cambridge, Department of Plant Sciences
Prof. Jim Haseloff, for giving us our one week introductory course to iGEM and synthetic biology, and continuous general advice
Dr. Susana Sauret-Gueto, for guidance in our experimental design and great advice on how to manage and arrange a lab for maximum efficiency. Also for regularly checking on our work and giving very useful feedback.
Dr. Moritz Meyer, for his help and support in the lab, not just with practical advice but with his constant sense of humour and unlimited patience.
Mr. Aleix Gorchs-Rovira, for always being able to answer our endless questions on PCR and cloning, as well as help with the golden gate design (another example of endless patience)
Dr. Payam Mehrshahi, for his always kind words, interest in the project development and help with protocols and analysis of results.
Dr. Doris Gangl, for advice on strain selection with regards to biological containment, and working with Chlamydomonas.
Dr. Francisco Navarro, for giving us insight on the measurement of fluorescence in Chlamydomonas reinhardtii.
Other sources of help
Prof. Saul Purton, University College London, for providing us a series of chloroplast transformation vectors as well as the TN72 Chlamydomonas reinhardtii strain, also for the help in wetlab with protocols and analysis of molecular cloning with Chlamydomonas.
Mr. Marco Larrea Alvarez, University College London, group member of Paul Purton’s lab for advice on working with Chlamydomonas as well as inviting us to UCL to troubleshoot some difficulties with the system and give us feedback of what parts would be useful to the research community.
Dr. Nicola Patron, Earlham Institute, for helping us with define our Cas9 strategy, and practical advice on the using CRISPR-Cas9 safely
Dr. Sarah Richardson, Ignition Genomics, for discussing our project with us.
Dr. Jim Ajioka, University of Cambridge, Department of Pathology, for discussing our project with us.
Prof. Tom Knight, Ginkgo Bioworks, for discussing our project with us.
WETLAB
University of Cambridge, Department of Plant Sciences
Dr. Moritz Meyer, for teaching us how to make TAP growth medium, culture Chlamydomonas reinhardtii, amplify DNA plasmids in bacteria and extract them, biolistics and for providing some consumables, especially for biolistics.
Dr Payam Mehrshahi and Mr. Aleix Gorchs-Rivera, for all the help and advice on the molecular cloning strategies and analysis of results.
Dr. Farhat Nazir, for laboratory supervision in the second half of our project, explaining how to use devices and what to be cautious about in lab procedures
Ms. Barbara Landamore, for giving a safety training, providing standard laboratory equipment and help with technical issues and organisation in the lab
Ms. Linda Silvestri, for helping us manage our lab consumables and hardware part orders, funds and donating her stocks of competent cells
Ms. Gabriela Doria, for helping us to run a few experiments
Dr. Katrin Geisler, helped us with the biolistics procedure
Other sources of help
Prof. Saul Purton, University College London, for supplying several C. reinhardtii strains and plasmids to start our work from
Dr. Orr Yarkoni, University of Cambridge Department of Pathology, supplied four fluorescent protein-marked plasmids which were included in our part library
Yan-kay Ho, Cambridge Consultants, for biology advice as well as information on the iGEM competition
Integrated DNA Technologies, for free synthesis of several genetic parts
Dutscher Scientific, for supplying hundreds of sterile pipette tips
New England Biolabs, for supplying competent E. coli cells and Gibson Assembly kits
Department of Plant Sciences, University of Cambridge for providing us with laboratory space and support from many of its members.
LiU iGEM team for sharing weekly reports and for skype meetings to follow up on each other’s work and advice on protocols
WETLAB
University of Cambridge, Department of Plant Sciences
Dr. Moritz Meyer, for teaching us how to make TAP growth medium, culture Chlamydomonas reinhardtii, amplify DNA plasmids in bacteria and extract them, biolistics and for providing some consumables, especially for biolistics.
Dr Payam Mehrshahi and Mr. Aleix Gorchs-Rivera, for all the help and advice on the molecular cloning strategies and analysis of results.
Dr. Farhat Nazir, for laboratory supervision in the second half of our project, explaining how to use devices and what to be cautious about in lab procedures
Ms. Barbara Landamore, for giving a safety training, providing standard laboratory equipment and help with technical issues and organisation in the lab
Ms. Linda Silvestri, for helping us manage our lab consumables and hardware part orders, funds and donating her stocks of competent cells
Ms. Gabriela Doria, for helping us to run a few experiments
Dr. Katrin Geisler, helped us with the biolistics procedure
Other sources of help
Prof. Saul Purton, University College London, for supplying several C. reinhardtii strains and plasmids to start our work from
Dr. Orr Yarkoni, University of Cambridge Department of Pathology, supplied four fluorescent protein-marked plasmids which were included in our part library
Yan-kay Ho, Cambridge Consultants, for biology advice as well as information on the iGEM competition
Integrated DNA Technologies, for free synthesis of several genetic parts
Dutscher Scientific, for supplying hundreds of sterile pipette tips
New England Biolabs, for supplying competent E. coli cells and Gibson Assembly kits
Department of Plant Sciences, University of Cambridge for providing us with laboratory space and support from many of its members.
LiU iGEM team for sharing weekly reports and for skype meetings to follow up on each other’s work and advice on protocols
ABOUT US
We are a team of Cambridge undergraduates, competing for the plant synthetic biology prize in iGEM 2016.
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FOLLOW US ON
LOCATION
Department of Plant Sciences,
University of Cambridge
Downing Street
CB2 3EA
