Team:CSU Fort Collins/NoteBook/JanThroughApr

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CSU iGEM



January Through April

1/4/16:
  • Ligated b0034 and c0061
  • Transformed Ligated mixture
  • Ran PCR results on a 2% gel
1/5/16:
  • Digested LuxR with EcoRI and SpeI
  • Performed Colony PCR on transformation from the 4th
1/11/16:
  • slr0435 Excision from Synechocystis sp. PCC6803 with PCR using Phusion(size 276 bp).
  • Ran PCR results on a 2% gel
  • Digested I0462 and E0030 with EcoRI and SpeI, R0062 and B0015 with EcoRI and XbaI, B0034 with SPeI and PstI, and C0061 with XbaI and PstI.
1/12/16:
  • Digested slr0435 and resuspended pSB1C3 with EcoRI and PstI
  • Ligated pSB1C3 and slr0435
  • Transformed ligation mixture
1/13/16:

Colony PCR performed on slr0435+pSB1C3 transformed colonies

Ran PCR results on 1% gel

2/6/16:

PCRed out BBA_J23106 out of glycerol stock 19 using Q5 master mix 19rxns

2/9/16:

Transformed Bba_K325909 from parts kit into DH5a cels

2/11/16:

To confirm that Bba_K325909 are functioning we tried to induce expression using L-arabinose

No luminescence was produced

3/24/16:

Set up O/N cultures of K1033929, K592009 and E1010 in 5 mL of LB with 5 uL of Cm34 antibiotic

3/29/16:

Ran promoters Sll027, and LrtA in a 1% gel

Sll0027
LrtA
4/6/16:

Ran Cpcg2 on 1% gel