Team:Tongji Shanghai/Model
Favorite Parts
- Team Fudan need tet-on or tet-of promoter to get tetO element and miniCMV as the promoter to drive their genes of interest. Vice professor Wenwen Jia in our school works on apparent heredity field, happens to have tet-on kit for his work. We made contact with Pro. Wenwen Jia and gained 3μl.
We also asked him for the associating kit that responses to panmycin and send the signal to promoter. Team Fudan used these two kits to PCR and continued their experiments. At the same time, Team Fudan had provided great help either. They built their kit ahead of us, and they generously provided almost all of the materials that we gonna need for kit submitting.(15 ml of chloromycetin powder,8 chloramphenicol resistance plates,80ml chloramphenicol resistance LB,Enzyme of XbaI and SpeI each 3μl)
Basic Parts
BBa_K1922001
- Approximately 80% of cancers are immortalized by constitutive activa-tion of telomerase to maintain telomeres throughout rapid cellular prolif-eration. HTert promoter is the promoter we cloned from human cell, which can drive the transcription of telomerase in human. We hoped that it can promote the transcription of the certain gene following it in tumor cells specifically, instead of in normal cells.
BBa_K1922003
- The cells of all tissues react to various stresses through the quick synthesization of HSPs.As the most important HSPs family. HSP70 has the highest temperature sensitivity and conservation. Having synthesized human hsp70 promoter, we hope it can drive the transcription of selected gene after heat shock at certain tem-perature. From the literature we finally set the preform condition as 42C for 1h
