Team:Cambridge-JIC/Results
RESULTS
It has been a summer of a lot of work, many mistakes and many corrections. Below you will find a bullet points of the broad overview of the successes and less successful aspects of our wetlab project. We then elaborate in text our experience and where we put our effort in. We have included “failures” and “mistakes” as we consider these to be useful to future iGEM teams who brave the challenge of working with plastids!
What we achieved this summer
- Generated a library of parts for Chlamydomonas reinhardtii chloroplasts
- Verified these parts through sequencing and/or restriction digest
- Verified our aadA antibiotic cassette in E.coli
- Biolistically transformed Chlamydomonas with gene gun
- Library-based design of homoplasmy strategy for chloroplasts
Outside the wetlab our work this summer has lead to
- DIY biolistics gene gun
- DIY Growth facility
- Modelling software for experimental design
What we did not achieve this summer
- DIY biolistics gene gun
- DIY Growth facility
- Modelling software for experimental design
Outside the wetlab our work this summer has lead to
- DIY biolistics gene gun
- DIY Growth facility
- Modelling software for experimental design
We assisted by providing Imperial iGEM with growth data from Chlamydomonas, for their computer model A.L.I.C.E. This computer model is aimed at producing a platform with the growth conditions for various different species, and ultimately for identifying possible co-cultures. Find out more here
We hope that the data we provide is especially useful for Imperial iGEM, as chlamydomonas takes significantly longer to grow that bacterial species.
Linköping University
The collaborations with Linköping University has been very helpful for both parties as we exchanged weekly reports through emails and Skype meetings. In depth discussions on the chasis we were working on, Chlamydomonas reinhardtii, were carried out. In addition, we exchanged views on Gibson assembly and other higher level assembly methods' protocols to help each other make an informed decision on the best approach for our project.
