Team:WashU StLouis/Notebook

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Notebook

Reading about labwork is almost as fun as doing labwork

June
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30

July
					1	2
3	4	5	6	7	8	9
10	11	12	13	14	15	16
17	18	19	20	21	22	23
24	25	26	27	28	29	30
31

August
	1	2	3	4	5	6
7	8	9	10	11	12	13
14	15	16	17	18	19	20
21	22	23	24	25	26	27
28	29	30	31

September
				1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30

October
						1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	31

❮❮ ❮ ❯❯ ❯

1 / 30

Stuff that happened on day 1

June 1st

2 / 30

Stuff that happened on day 2

June 2nd

3 / 30

• Made 1L of LB Media

June 3rd

4 / 30

Stuff that happened on day 4

June 4th

5 / 30

Stuff that happened on day 5

June 5th

6 / 30

June 6th

7 / 30

• Performed gDNA Extraction on E. Coli MG1655 and Synechocystis 6803

June 7th

8 / 30

• Designed PCR primers for pgk, pck, fldA, petF, ATP Synthase 1, and ATP Synthase 2

June 8th

9 / 30

Stuff that happened on day 9

June 9th

10 / 30

• Primers Arrived
• Ran PCR on ATP Synthase 2 , pgk, fldA, petF, and pdCas9 plasmid
• Ran all PCR products on gel and recovered ATP Synthase 2, fldA, and petF
• Ran gradient PCR on pdCas9 plasmid

June 10th

11 / 30

Stuff that happened on day 11

June 11th

12 / 30

Stuff that happened on day 12

June 12th

13 / 30

Made and ran gel for pdCas9

• Ran PCR on ATP Synthase 1, pgk, and pck
• Performed gel extraction on yesterday’s ATP Synthase 2
• Ran pck, pgk, and yesterday’s gradient pdCas9 PCR products on gel and recovered all genes
• Performed gel extraction on pdCas9, fldA, petF
• Ran PCR on psc101 and psc102 plasmid
• Ran psc101 and psc102 PCR products on gel - were recovered
• Ran gradient PCR on ATP Synthase 1
• Made agar plates (forgot the antibiotic)
• Ran golden gate one pot assembly for fldA petF genes and the pdCas9 plasmid

June 13th

14 / 30

• Ran ATP Synthase 1 PCR products on gel - no bands seen
• Gel Extraction for pgk, pck, psc101, and psc102 plasmid
• Ran PCR on ATP Synthase 1 (3rd times the charm)
• Ran PCR products on gel - WE SAW BANDS! ATP SYNTHASE 1 RECOVERED
• Reran PCR on pgk, pck, psc101, and psc102 plasmid because we did not get high enough concentration during gel Extraction
• Made new agar plates (with the chloramphenicol)
• Transformed the petF, fldA plasmids into competent cells
• Plated colonies on agar plates and set in incubator

June 14th

15 / 30

• Checked for colonies (both genes were successful)
• Picked colonies and incubated overnight
• Ran yesterday’s PCR products on gel - bands were light but all were recovered
• Gel Extraction for pgk, pck, psc101, and psc102 plasmid
• Reran PCR on pgk, pck, psc101, and psc102 plasmid because we did not get high enough concentration during gel extraction
• Ran PCR products on gel - all were recovered
• Gel Extraction for pgk, pck, psc101, and psc102 plasmid

June 15th

16 / 30

Stuff that happened on day 16

June 16th

17 / 30

Stuff that happened on day 17

June 17th

18 / 30

Stuff that happened on day 18

June 18th

19 / 30

Stuff that happened on day 19

June 19th

20 / 30

Stuff that happened on day 20

June 20th

21 / 30

Stuff that happened on day 21

June 21st

22 / 30

Stuff that happened on day 22

June 22nd

23 / 30

Stuff that happened on day 23

June 23rd

24 / 30

Stuff that happened on day 24

June 24th

25 / 30

Stuff that happened on day 25

June 25th

26 / 30

Stuff that happened on day 26

June 26th

27 / 30

Stuff that happened on day 27

June 27th

28 / 30

Stuff that happened on day 28

June 28th

29 / 30

Stuff that happened on day 29

June 29th

30 / 30

Stuff that happened on day 30

June 30th

1 / 31

Stuff that happened on day 31

July 1st

2 / 31

Stuff that happened on day 32

July 2nd

3 / 31

Stuff that happened on day 33

July 3rd

4 / 31

Stuff that happened on day 34

July 4th

5 / 31

Stuff that happened on day 35

July 5th

6 / 31

Stuff that happened on day 36

July 6th

7 / 31

Stuff that happened on day 37

July 7th

8 / 31

Stuff that happened on day 38

July 8th

9 / 31

Stuff that happened on day 39

July 9th

10 / 31

Stuff that happened on day 40

July 10th

11 / 31

Stuff that happened on day 41

July 11th

12 / 31

Stuff that happened on day 42

July 12th

13 / 31

Stuff that happened on day 43

July 13th

14 / 31

Stuff that happened on day 44

July 14th

15 / 31

Stuff that happened on day 45

July 15th

16 / 31

Stuff that happened on day 46

July 16th

17 / 31

Stuff that happened on day 47

July 17th

18 / 31

Stuff that happened on day 48

July 18th

19 / 31

Stuff that happened on day 49

July 19th

20 / 31

Stuff that happened on day 50

July 20th

21 / 31

Stuff that happened on day 51

July 21st

22 / 31

Stuff that happened on day 52

July 22nd

23 / 31

Stuff that happened on day 53

July 23rd

24 / 31

Stuff that happened on day 54

July 24th

25 / 31

Stuff that happened on day 55

July 25th

26 / 31

Stuff that happened on day 56

July 26th

27 / 31

Stuff that happened on day 57

July 27th

28 / 31

Stuff that happened on day 58

July 28th

29 / 31

Stuff that happened on day 59

July 29th

30 / 31

Stuff that happened on day 60

July 30th

31 / 31

Stuff that happened on day 61

July 31st

1 / 31

Stuff that happened on day 62

August 1st

2 / 31

Stuff that happened on day 63

August 2nd

3 / 31

Stuff that happened on day 64

August 3rd

4 / 31

Stuff that happened on day 65

August 4th

5 / 31

Stuff that happened on day 66

August 5th

6 / 31

Stuff that happened on day 67

August 6th

7 / 31

Stuff that happened on day 68

August 7th

8 / 31

Stuff that happened on day 69

August 8th

9 / 31

Stuff that happened on day 70

August 9th

10 / 31

Stuff that happened on day 71

August 10th

11 / 31

Stuff that happened on day 72

August 11th

12 / 31

Stuff that happened on day 73

August 12th

13 / 31

Stuff that happened on day 74

August 13th

14 / 31

Stuff that happened on day 75

August 14th

15 / 31

Stuff that happened on day 76

August 15th

16 / 31

Stuff that happened on day 77

August 16th

17 / 31

Stuff that happened on day 78

August 17th

18 / 31

Stuff that happened on day 79

August 18th

19 / 31

Stuff that happened on day 80

August 19th

20 / 31

Stuff that happened on day 81

August 20th

21 / 31

Stuff that happened on day 82

August 21st

22 / 31

Stuff that happened on day 83

August 22nd

23 / 31

Stuff that happened on day 84

August 23rd

24 / 31

Stuff that happened on day 85

August 24th

25 / 31

Stuff that happened on day 86

August 25th

26 / 31

Stuff that happened on day 87

August 26th

27 / 31

Stuff that happened on day 88

August 27th

28 / 31

Stuff that happened on day 89

August 28th

29 / 31

Stuff that happened on day 90

August 29th

30 / 31

Stuff that happened on day 91

August 30th

31 / 31

Stuff that happened on day 92

August 31st

1 / 30

Stuff that happened on day 93

September 1st

2 / 30

Stuff that happened on day 94

September 2nd

3 / 30

Stuff that happened on day 95

September 3rd

4 / 30

Stuff that happened on day 96

September 4th

5 / 30

Stuff that happened on day 97

September 5th

6 / 30

Stuff that happened on day 98

September 6th

7 / 30

Stuff that happened on day 99

September 7th

8 / 30

Stuff that happened on day 100

September 8th

9 / 30

Stuff that happened on day 101

September 9th

10 / 30

Stuff that happened on day 102

September 10th

11 / 30

Stuff that happened on day 103

September 11th

12 / 30

Stuff that happened on day 104

September 12th

13 / 30

Stuff that happened on day 105

September 13th

14 / 30

Stuff that happened on day 106

September 14th

15 / 30

Stuff that happened on day 107

September 15th

16 / 30

Stuff that happened on day 108

September 16th

17 / 30

Stuff that happened on day 109

September 17th

18 / 30

Stuff that happened on day 110

September 18th

19 / 30

Stuff that happened on day 111

September 19th

20 / 30

Stuff that happened on day 112

September 20th

21 / 30

Stuff that happened on day 113

September 21st

22 / 30

Stuff that happened on day 114

September 22nd

23 / 30

Stuff that happened on day 115

September 23rd

24 / 30

Stuff that happened on day 116

September 24th

25 / 30

Stuff that happened on day 117

September 25th

26 / 30

Stuff that happened on day 118

September 26th

27 / 30

Stuff that happened on day 119

September 27th

28 / 30

Stuff that happened on day 120

September 28th

29 / 30

Stuff that happened on day 121

September 29th

30 / 30

Stuff that happened on day 122

September 30th

1 / 31

Stuff that happened on day 93

October 1st

2 / 31

Stuff that happened on day 94

October 2nd

3 / 31

Stuff that happened on day 95

October 3rd

4 / 31

Stuff that happened on day 96

October 4th

5 / 31

Stuff that happened on day 97

October 5th

6 / 31

Stuff that happened on day 98

October 6th

7 / 31

Stuff that happened on day 99

October 7th

8 / 31

Stuff that happened on day 100

October 8th

9 / 31

Stuff that happened on day 101

October 9th

10 / 31

Stuff that happened on day 102

October 10th

11 / 31

Stuff that happened on day 103

October 11th

12 / 31

Stuff that happened on day 104

October 12th

13 / 31

Stuff that happened on day 105

October 13th

14 / 31

Stuff that happened on day 106

October 14th

15 / 31

Stuff that happened on day 107

October 15th

16 / 31

Stuff that happened on day 108

October 16th

17 / 31

Stuff that happened on day 109

October 17th

18 / 31

Stuff that happened on day 110

October 18th

19 / 31

Stuff that happened on day 111

October 19th

20 / 31

Stuff that happened on day 112

October 20th

21 / 31

Stuff that happened on day 113

October 21st

22 / 31

Stuff that happened on day 114

October 22nd

23 / 31

Stuff that happened on day 115

October 23rd

24 / 31

Stuff that happened on day 116

October 24th

25 / 31

Stuff that happened on day 117

October 25th

26 / 31

Stuff that happened on day 118

October 26th

27 / 31

Stuff that happened on day 119

October 27th

28 / 31

Stuff that happened on day 120

October 28th

29 / 31

Stuff that happened on day 121

October 29th

30 / 31

Stuff that happened on day 122

October 30th

31 / 31

Stuff that happened on day 122

October 31st

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