Team:Tongji Shanghai/Safety
Fig1.most of our work is finished in the laminar bench
Fig.2 most of our equipment has been treated by high-pressure steam sterilization.
Fig.3 the cells we used in our experiment is tested as mycoplasma free.
Fig.4 we used the UV light to sterilize the laminar bench everytime before and after our experiment. In the experiment, we use spirit lamp to provide a microorganism-free environment.
Fig.5 the waste liquid is treated with sterile liquid to avoid causing harm to the environment.
1. lab safety
- Safety is prior to all other stuffs especially in the lab.
- The Administrative office of Laboratory and Equipment in Tongji University is responsible for the safety of biology labs. Before we freshmen get into the lab and do experiments, we must take some courses offered by Administrative office of Laboratory and Equipment and have several tests to qualify our safety training on the Tongji University website.
- The Administrative office of Laboratory and Equipment in Tongji University is responsible for the safety of biology labs. Before we freshmen get into the lab and do experiments, we must take some courses offered by Administrative office of Laboratory and Equipment and have several tests to qualify our safety training on the Tongji University website.
- 1) Experiment participants need to understand the experiment completely.
- 2) Wear rubber gloves in all experimenters.
- 3) Necessary steps should be performed in bio-safety cabinet.
- 4) Fire, electric heaters, microwave oven should not be left while being used.
- 5) Sterilize all liquid waste and solid waste containing living organism.
- 6) Sterilize the lab using UV-light every week.
- 7) The last person to leave the lab should make sure that water, electricity, gas, and air conditioner has been closed, and doors and windows has been locked before leaving.
- Even though all the organism we work with are not harmful for the environment and health, we still assume that they may threaten the ecology, so we wear rubber gloves and lab coat when we do the experiments to keep it from direct touching with human body. We also do all the experiments with these living organism in the laminar flow bench to prevent those organism from getting into the outside air. After we do the experiments, we use NaclO solution to sterilizing waste such as the used agar plate and the used tips. What's more, everytime after we finish our work, we use UV light to sterilize the lab.
2. environmental safety
- In our project, we use 6 kinds of organism, hcc1997,hela and 293T cell line plus virus ranked in the organisms of Level 2 risk group, while E.coli which is ranked in the organisms of Level 1 risk goup and the mouse which is kept in SPF level. The three cell lines is tested as mycoplasma free. All the risk level organism is treated with sterile liquid to avoid causing harm to the environment.
3. biosafety
- The cell toxicity experime nt shows that the toxicity of the AuNRs is dose-related. When the AuNRs concentration is low, it shows no obvious cell toxicity, but it exhibits cell toxicity with the concentration increase. The cell toxicity is the result of the AuNRs can induce cell apoptosis and autophagy by damaging mitochondria and activating intracellular reactive oxygen species (ROS) [1]. Surface modifications have been extensively used to improve the biocompatibility and stability of gold nanorods. And our results show that with the surface modification of PEG, the toxicity of the AuNRs decreased dramatically. Also the mice we injected AuNRs do not show any different from the control group for now. In a word, all the results testify our material have well-controlled biosafety both in material stability and its cell toxicity.
- [1] Wan J, Wang J H, Liu T, et al. Surface chemistry but not aspect ratio mediates the biological toxicity of gold nanorods in vitro and in vivo[J]. Scientific reports, 2015, 5
