Team:UNSW Australia/Results

Hypervesiculation Strain Library

Our first step was to generate some strains of E. coli that our research suggested would hypervesiculate. These are shown below:

Assembly of g3p, tolR, PP-RFP and INPNC-GFP Expression Plasmids

Our knock-in mutations were achieved by expressing g3p, TolR, PP-RFP, or INPNC-GFP in a T7-promoter driven vector (either pET or pRSF Duet). Final assembly of these plasmids was achieved using Gibson assembly.

Figure 2A: Gel electrophoresis showing PP-RFP (lane 2, 1203bp) and INPNC-GFP (lane 3, 2133bp) inserted into the pET Duet expression plasmid. Lane 1 (554) is a negative control of pET with no insert. Figure 2B: Gel electrophoresis showing g3p inserted into the pRSF Duet expression plasmid (649bp). Lane 1 (551bp) is a negative control of pRSF with no insert. Figure 2C: Gel electrophoresis showing TolR (Lane 1, 687bp) inserted into the pET Duet expression plasmid. Lane 2 (554) is a negative control of pET with no insert.