Team:Valencia UPV/HP/Silver
May 18, 2016
Take glycerinated cultures of C58 Agrobacterium with dsRED from GoldenBraid Collection. Prepare broth culture (5 mL LB + 5 μL kanamycin + 5 μL Rifampin) 1:1000 and incubate overnight at 28°C.
May 19, 2016
Refresh previously made culture by inoculating 10 μL in a new culture medium.
