Team:UCLA/Notebook

• Projects

  Protein Cages Super Soldier Systems Interlab Study

• Parts

  Basic Parts Composite Parts Part Collection

• Outreach

  Human Practices Collaborations

• Judging

  Medal Requirements Safety

• Team

  Meet the Team Attributions

JOURNAL

Projects

CDI

Protein Cages

Week

1

Construction of ATCC 13048 Loci 2 in pSB1C3

Isolation of template DNA from EC93, EC869, and ATCC 13048; PCR of Gibson fragments for EBL2 in pSB1C3 construct

Jun

27

Inoculation of strains obtained from Dr. Christopher Hayes

Inoculated overnight cultures of:

• EPI100 w/ EC93 cosmid
• X90 w/ EC869 cosmid
• ATCC 13048

Conditions:

• 10mL LB
• 10uL ampicillin
• 37 C for 16h shaking

Jun

28

Isolation of template DNA

Miniprep of EC93 and EC869

• EC93 #1: 145.42 ng/μL
• EC93 #2: 198.85 ng/μL
• EC869 #1: 90.81 ng/μL
• EC869 #2: 45.95 ng/μL

gDNA isolation of ATCC 13048

• 34.87 ng/μL

Week

1

Title For The Week

Description blah blah blah

Jun

24

Title for the day (e.g. "Cloning Mutants")

Today we did cloning. fuck cloning. blah blah blah.