Team:UCLA/Notebook
JOURNAL
Projects
CDI
Protein Cages
Week
1
Construction of ATCC 13048 Loci 2 in pSB1C3
Isolation of template DNA from EC93, EC869, and ATCC 13048; PCR of Gibson fragments for EBL2 in pSB1C3 construct
Jun
27
Inoculation of strains obtained from Dr. Christopher Hayes
Inoculated overnight cultures of:
- EPI100 w/ EC93 cosmid
- X90 w/ EC869 cosmid
- ATCC 13048
Conditions:
- 10mL LB
- 10uL ampicillin
- 37 C for 16h shaking
Jun
28
Isolation of template DNA
Miniprep of EC93 and EC869
- EC93 #1: 145.42 ng/μL
- EC93 #2: 198.85 ng/μL
- EC869 #1: 90.81 ng/μL
- EC869 #2: 45.95 ng/μL
gDNA isolation of ATCC 13048
- 34.87 ng/μL
Gibson primers designed for EBL1 and EBL2 toxin switched constructs in pSB1C3 and pSC101
Jun
29
Isolation of template DNA
Miniprep of EC93 and EC869
- EC93 #1: 145.42 ng/μL
- EC93 #2: 198.85 ng/μL
- EC869 #1: 90.81 ng/μL
- EC869 #2: 45.95 ng/μL
gDNA isolation of ATCC 13048
- 34.87 ng/μL
Gibson primers designed for EBL1 and EBL2 toxin switched constructs in pSB1C3 and pSC101
Week
1
Title For The Week
Description blah blah blah
Jun
24
Title for the day (e.g. "Cloning Mutants")
Today we did cloning. fuck cloning. blah blah blah.
