MITO-MORHPIN' POWER YEAST!
Controlling Translation in Yeast Mitochondria
Welcome to Team RHIT's iGEM 2016 Wiki!.
PERHAPS A PICTURE HERE? MAYBE THE ELEPHANTS WILL SUFFICE.
Hailing from Terre Haute, Indiana, the Rose-Hulman iGEM team continues its work from last year in the ways of controlling aerobic respiration through mitochondrial ribosomal proteins. ===HERE WOULD BE A NICE PLACE TO SUM UP WHAT THEY DID LAST YEAR AND HOW WE ADDED TO IT.===
WE'RE GOING TO PUT A BRIEF ABSTRACT HERE. LIKE, NOT THE EXACT SAME THING WE COPY-PASTE EVERYWHERE, BUT, LIKE, MAYBE A SMIDGE LESS FORMAL. HELL, THIS MAY EVEN BE AN IMAGE.
Saccharomyces cerevisiae is used in a variety of valuable industrial processes. Its ability to grow and replicate utilizing aerobic respiration or fermentation, and its ability to survive without functional mitochondria, provide opportunities to control or alter mitochondrial function to optimize production processes or develop novel cellular subsystems in the mitochondrial space. To begin exploring these opportunities, we adapted two commonly used yeast expression vectors to the RFC 10 standard and used them to express yEGFP preceded by the mitochondrial localization signal from the mitoribosomal protein mRPS12. In addition, we attempted to demonstrate that aerobic respiration can be controlled by manipulating the expression of mRPS12. Without mRPS12, yeast mitochondria lack a functioning ribosome and become unable to produce proteins necessary for aerobic respiration. By expressing the mRPS12 protein in a haploid mRPS12 knockout strain, we investigated its usefulness as a means of regulating aerobic respiration in Saccharomyces cerevisiae.
Stick around our wiki for a bit, learn about our project, have a good time! :D
stuff to do
Meet the team
Look at our notebook
Other stuff YAYAYAYAYA
