Team:RHIT/Description
Mito Morphin' Power Yeast Project Description
General Overview
As an extension of Team RHIT’s 2015 iGEM project, our goal was to verify the function of MRPS12's mitochondrial localization signal (mls), as well as restore the function of mitochondria in the yeast species Saccharomyces cerevisiae which were deficient in the gene.
Basic Trajectory and Parts
The first portion of the project is to verify the function of the two parts submitted by last year's team. These parts are the mRPS12 translational unit (tu) and the mRPS12 mls. We started by creating yeast vectors that are compatible with the BioBrick standard prefix and suffix. These modified standard vectors facilitated the cloning of our parts into yeast cells and should make working with S. cerevisiae much easier for other iGEM teams in the future. The function of mls will be verified by linking it to GFP. The function of mRPS12 will be verified by transforming mRPS12 knock out yeast and testing for restored mitochondrial function using both fermentable and nonfermentable carbon sources.
Results
