Team:HUST-China
Abstract
WHAT IS SIGNAL FILTER ?
This year, we, HUST-China, design a functional gene expression toolkit. The systems we design can not only be adaptable to any input and output, but also can change its threshold to meet the requirements from different project purposes.
Normally, gene expression level depends on the input signals' intensity but it always cna't be strong enough, so it may be hard for former expression systems to deal with realistic problems.
Our toolkit can sense even pulse signal to reach its stable expression state and with validly characterized circuits, it will definitely help new iGEMers.
WHY DO WE USE IT ?
This year, we, HUST-China, design a functional gene expression toolkit. The systems we design can not only be adaptable to any input and output, but also can change its threshold to meet the requirements from different project purposes.
Normally, gene expression level depends on the input signals' intensity but it always cna't be strong enough, so it may be hard for former expression systems to deal with realistic problems.
Our toolkit can sense even pulse signal to reach its stable expression state and with validly characterized circuits, it will definitely help new iGEMers.
HOW DOES IT WORK ?
This year, we, HUST-China, design a functional gene expression toolkit. The systems we design can not only be adaptable to any input and output, but also can change its threshold to meet the requirements from different project purposes.
promoter RE can be activated by CII . Ftsh normally degrade CII, while CIII serves as an inhibitor of Ftsh to free CII. CI can function as an inhibitor to block pR, while Cro can block pRM. All of these parts and the interations between them can constitute three stable states and finally lead to different gene expressions.
When signal 1 (ON) comes, promoter RD29A drives expression of SnRK2.2, which can phosphorylate a large quantity of ABF2 in a short time by enzyme catalysis , then enhance pRD29A to turn on the gene of interest's expression. When signal 2 (OFF) comes, gene PP2CA expresses, dephosphorylates ABF2 and inactivates SnRK2.2, thus turning the system into OFF state.
Small in scale, big in thoughts! This summer, we held a fabulous HUST-Cheering! conferrence. See more details in our Human Practices page.
Fluorescence intensity measurement is the main characterization method this year. We employed GFP and RFP as reporters to build valid characterization for our circuits. See more results in our Results page.
We simulated our project in cellular level and Intestinal level to help us figure out the feasibility of it and guide us the direction of further improvement. See more results in our Model page.
