| Wet Lab Results |
| Cloning Pct in pSB1C3 |
|
| Cloning PhaC in pSB1C3 |
|
| Constructing the operon PhaC-Pct |
No |
| Cloning in pSEVA to express PLA enzymes |
No |
| Constructing BioBrick IPTG promoter |
|
| Constructing Biobrick CH promoter |
|
| Characterizing BioBrick IPTG promoter (Achieved by iGEM Paris Bettencourt) |
|
| Characterizing BioBrick CH promoter |
|
| Improving Pct BioBrick |
|
| Improving PhaC BioBrick |
|
| Deleting PhaC WT gene |
No |
| Studying growth rates with glucose, glycerol and benzoate for selecting carbon source |
|
| Modeling Results |
| Representing a modeling strategy based on bio-circuits in order to control metabolic pathways dynamically |
|
| Doing Kappa model in order to study and solve the dynamic regulation system |
|
| Reconstructing the genome-scale metabolic network of our chassis containing PLA production pathway |
|
| Optimizing the metabolic network using FBA studying difference growth conditions and carbon sources and discovering the bathway bottlenecks |
|
| Collaboration Results |
| Performing an HPLC and MS for Paris Bettencourt |
|
| Studying growth rate for Imperial College |
|
| Integration of Human Practices |
| Conception of an optimizing Bioprocess and construction of key parts in a DIY manner |
|
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