Team:Tianjin/Basic Part
Basic Parts
Basic Parts For R-R System
| Biobrick | Type | Description | Length |
|---|---|---|---|
| BBa_K2110000 | Terminator | CYC1 Terminator | 248 |
| BBa_K2110012 | Regulatory | PGK1 promoter | 781 |
| BBa_K2110017 | Signalling | α-factor signal peptide | 267 |
| BBa_K2110001 | Regulatory | TPA inducible Leader sequence | 96 |
| BBa_K2110002 | Coding | TPA positive feedback regulatory gene | 807 |
| BBa_K2110003 | Coding | TPA transporting protein gene | 1380 |
| BBa_K2110004 | Coding | D-alanyl-D-alanine dipeptidase gene | 582 |
Basic Parts For Microbial Consortium
| Biobrick | Type | Description | Length |
|---|---|---|---|
| BBa_K2110009 | Coding | phage P22 holins | 327 |
| BBa_K2110010 | Coding | phage P22 auxiliary lysis factors | 438 |
| BBa_K2110011 | Coding | phage P22 endolysins | 441 |
| BBa_K2110013 | Coding | PETase enzyme after codon optimization for bacillus subtilis | 885 |
| BBa_K2110014 | Coding | MHETase enzyme after codon optimization for bacillus subtilis | 1848 |
| BBa_K2110015 | Coding | The key gene for accumulation of PHA in P.putida KT2440 | 978 |
| BBa_K2110016 | Coding | The key gene for utilization of ethylene glycolin P.putida KT2440 | 1326 |
Basic Parts For Protein Modification
| Biobrick | Type | Description | Length |
|---|---|---|---|
| BBa_K2110800 | Basic | PETase | 795 |
| BBa_K2110100 | Basic | PETase site-directed mutant A240P | 795 |
| BBa_K2110101 | Basic | PETase site-directed mutant S207T | 795 |
| BBa_K2110102 | Basic | PETase site-directed mutant M154L | 795 |
| BBa_K2110103 | Basic | PETase site-directed mutant N205V | 795 |
| BBa_K2110104 | Basic | PETase site-directed mutant R90A | 795 |
| BBa_K2110105 | Basic | PETase site-directed mutant I208V | 795 |
| BBa_K2110106 | Basic | PETase site-directed mutant S238V | 795 |
| BBa_K2110107 | Basic | PETase site-directed mutant L117F | 795 |
| BBa_K2110108 | Basic | PETase site-directed mutant I208F | 795 |
| BBa_K2110109 | Basic | PETase site-directed mutant S238F | 795 |
