Difference between revisions of "Team:BostonU/Safety"

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<p>Welcome to the BostonU Project Gemini Safety Review.</p>
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<p>The greatest safety concerns that arose during our project surrounded the chasses used for both cloning and assaying our systems. Specifically, our chasses encompassed two distinct biological safety levels: BSL 1 and BSL2. Each level required a different safety protocols as well training, of which received from both the RIMS program at Boston University and our Mentors.
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All cloning done by our team was done in Top10 Chemically Competent E. coli. For this reason, the work was all done on the benches of the BSL 1 area of the laboratory. This meant all scientist wore pants, closed toed shoes, lab coats, and gloves. Our samples were either stored in parafilmed plates, in -80 centigrade stasis, or were bleached. All chemicals used during the cloning process (i.e. miniprep waste and ethidium bromide gels) were disposed of in appropriately labeled waste containers located around the lab.</p>  
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>Our work with BSL2 materials centered around the HEK293FT cells. While HEK cells are generally a BSL cell line, the addition of a SV40 virus into out cells increased the biological safety concern. SV40 poses a cancer risk to those scientists working with it. With that being noted, all BSL 1 attire was worn in the BSL 2 Tissue Culturing room, as well as state certified eye protection. In addition to bleaching all samples upon the completion of experiments, all work was done within biological hoods with media traps. Every surface within those hoods was sterilized both preceding and at the conclusion of work with 70% ethanol.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Revision as of 19:18, 27 September 2016