Difference between revisions of "Team:Tianjin/Note/6803"

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    火影忍者十分钟疾风传。了解其中最佳CP从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。<br />从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。<br />从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。<br />从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。<br /></p>
                  <li><i>19</i> amplification at 65.0°C with 19.rev/fwd primes</li>
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      <li>PCR worked, positive control worked, no amplification of <i>19</i></li>
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<li><i>15</i> amplification at 65.0°C with 15.rev/fwd primes</li>
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      <li>PCR worked, positive control worked, no amplification of <i>15</i></li>
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<li>A product length of 900 bp was expected. The gel shows that most plasmids seem to be correct.</li>
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<li>This result was confirmed by sequencing.</li>
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<li>The fragments of <i>19</i> and <i>15</i> were purified with PCR Purification Kit.</li>
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<b>Ligation of <i>15</i> with <i>19</i><b><br/>
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<li>Fragment of <i>19</i> was phosphorylated and then purified with DNA Purification Kit.</li>
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<li><i>19</i> was ligated into T vectors via TA clone and transformed into E.coli via heat shock.</li>
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<li>A colony PCR was performed with five colonies</li>
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  <li>Gel electrophoresis showed that 5 colonies were positive for insertion of <i>19</i>.</li>
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<li>Two of these colonies containing <i>19</i> were used to inoculate overnight cultures.</li>
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  <li><i>15</i> gene fragment was phosphorylated.</li>
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<li>Plasmids containing T vector with <i>19</i>(pT-19)were isolated using a miniprep kit.</li>
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<li>Mono-restriction digest of pT-19 with stu I </li>
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<li>The enzyme-digested product was dephosphorylation.</li>
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<li>Dephosphorylated plasmid and phosphorylated gene <i>15</i> were connected.</li>
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  <li>Ligation product was transformed into E.coli via heat shock.</li>
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<li>A colony PCR was performed with twelve colonies.</li>
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      <li>Gel electrophoresis showed that 2 colonies were positive for connecting the plasmid and gene fragment.</li>
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      <li>These two colonies were used to inoculate overnight cultures.</li>
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<li>Plasmids with correct sequence of  <i>19-15</i>  were isolated using a miniprep kit.</li>
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<li>Mono-restriction digest of pT-19-15 with Nru I</li>
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<li>The enzyme-digested product was dephosphorylation.</li>
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Revision as of 14:11, 28 September 2016

TEAM TIANJIN


Team Tianjin-Attribution

Week3(8/29/2016-9/4/2016)

  • pMV-G19
  • pMV-G15
  • Colonies were used to inoculate overnight cultures.
  • Plasmids were isolated using a miniprep kit.
  • Amplification of 19 and 15 with Q5 High-Fidelity DNA Polymerase out of E.coli

    火影忍者十分钟疾风传。了解其中最佳CP从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。
    从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。
    从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。
    从小就是青梅竹马。没有月光,除了远处县城依稀的灯光和偶尔驶过的车灯外,便是一片漆黑,伸手不见五指,只能在满天繁星的映衬下依稀看见些随风而动的树影。而这,正是拍摄银河的最佳时机。

    Show More IMG_2956

    查看Team Tianjin全部实验


    火影忍者疾风传

    终于来到喀纳斯——中国最西北的角落,此次西北之行可到达的最远处。在这个纬度高达 48 度的地方,山坡上的植被更加茂密;太阳下落得很慢,阳光以一个非常低的角度横扫过来,跨过群山,透过树木,在地上都投出长长的影子,非常好看。

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    火影忍者十分钟疾风传

    告别禾木乡,继续向喀纳斯方向前行,途中经过冲乎尔乡。这里是一个天然牧场,坐落在小盆地,群山环抱,乡民主要以放牧为生。果然,不时就能看见牧羊人赶着几百头“阿勒泰大尾羊”在公路上浩浩荡荡地走着,一路扬起滚滚烟尘,甚是壮观。
    IMG_2861

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    北疆树林

    继续在铁热克提附近沿着蜿蜒的小道行摄,不知不觉间便绕到这座高山的另一侧。这里的杨树长得更高耸茂密,西斜的阳光恰到好处地将树梢照得金黄透亮,一道道树影投射在绒毛地毯般的甸上,构成近乎完美的光影,令人心旷神怡,不舍离。
    IMG_2696

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    Notice: This page is currently under construction. Contents in this page are temporaory and will be modified several times before the final release.     — 2016 iGEM Team Tianjin