Revision as of 11:31, 12 October 2016

TEAM TIANJIN

Worthy

Experiment

Fig.1. Structure of part BBa_K339007

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 <br/><p style="font-size:15px;text-align:center">Fig.1. Structure of part <i><i>BBa_K339007</i></i></p>
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-<h3><b>1. Reporting System</b></h3>
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-								<p style="font-size:18px">The basis of our reporting system is the part <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K339007" target="_blank"><i>BBa_K339007</i></a>, Designed by Emily Hicks from Group iGEM10_Calgary. This part can sense the CpxR protein, which will form spontaneously in <i>E.coli</i> when inclusion body and misfolding protein present in the periplasm of <i>E.coli</i>, and then start expressing RFP so that we can detect red fluorescence. As we all know, the inclusion body will inevitably form when we overexpress heterologous protein like PETase in <i>E.coli</i>. Therefore, the emission of red fluorescence can report the overexpression of PETase. What is more, this device can be modified to report overexpression of any heterologous protein only if the PETase gene is replaced by another heterologous gene. After the red fluorescence is detected, we could start the purification of protein.</p>
+								<img src="https://static.igem.org/mediawiki/2016/3/32/T--Tianjin--R-R_system1.jpg" alt="desktop">
+<br/><p style="font-size:15px;text-align:center">Fig.1. Structure of part <i><i>BBa_K339007</i></i></p>
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+								<img src="https://static.igem.org/mediawiki/2016/3/32/T--Tianjin--R-R_system1.jpg" alt="desktop">
+<br/><p style="font-size:15px;text-align:center">Fig.1. Structure of part <i><i>BBa_K339007</i></i></p>
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Difference between revisions of "Team:Tianjin/Description"

Revision as of 11:31, 12 October 2016

Experiment