Difference between revisions of "Team:GDSYZX-United/Demonstrate"

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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project </li>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h5>Inspiration</h5>
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<p>See how other teams presented their results.</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/extract _dna">Extract DNA in arabidopsis thaliana</a></li>
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<h1><strong>Demonstrate</strong></h1>
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                                </div>
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                            </div>
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<h2><strong>The trascription level of HHL1 in transgentic Arabidopsis</strong></h2>
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<p><div class="text_font">In the compete transgenic Arabidopsis，the result is very similar. The transcription controlled by pHhl1 is also very low. However, under high light, the transcription  controlled by three photosensitive promoters are also very high, and the highest is phyB, and then is pCop1.</div></p>
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<p><div class="text_font">These suggested that our parts can also function in the compete plants, and the result is similar.</div></p>
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<div align="center">
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<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/f/f1/T--GDSYZX-United--demonstrate1.jpg"/>
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</div>
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<p><div class="text_font">Fig.The transcription level of HHL1 in transgentic Arabidopsis</div></p>
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<table class="table" style="text-align:center">
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<thead>
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<th></th>
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<th colspan="2" style="text-align:center">Growth light</th>
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<th colspan="2" style="text-align:center">High light</th>
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</thead>
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<tbody>
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<tr>
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<td></td>
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<td>REL</td>
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<td>SE</td>
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<td>REL</td>
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<td>SE</td>
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</tr>
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<tr>
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<td>Phhl1</td>
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<td>1</td>
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<td>0.07</td>
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<td>1.1</td>
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<td>0.17</td>
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</tr>
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<tr>
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<td>Pcop1</td>
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<td>1</td>
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<td>0.13</td>
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<td>15.1</td>
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<td>1.86</td>
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</tr>
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<tr>
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<td>Ppif1</td>
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<td>1</td>
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<td>0.26</td>
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<td>10.9</td>
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<td>0.61</td>
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</tr>
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<tr>
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<td>PphyB</td>
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<td>1</td>
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<td>0.19</td>
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<td>20.6</td>
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<td>1.96</td>
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</tr>
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</tbody>
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</table>
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<h2><strong>Photosynthetic efficiency in transgentic Arabidopsis</strong></h2>
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<p><div class="text_font">We transformed four vectors with stably expressions into Arabidopsis, then detected the photosynthetic efficiency of transgenic Arabidopsis. </div></p>
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<p><div class="text_font">&nbsp;&nbsp;1）Under growth light condition, the chloroplast of the plants that transformed in four different vectors were not damaged, so the red fluorescence of the chloroplasts have no obvious difference and maintain a high level.</div></p>
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<p><div class="text_font">&nbsp;&nbsp;2）However, under high light condition, the construction of chloroplasts were damaged in different level although the protein HHL1 exsist. So the red fluorescence of chloroplast had decreased, but the decrease level is quit different, the lower the fluorescence is, the stronger the damage is. </div></p>
 +
<p><div class="text_font">From the diagram we can know that the fluorescence of plants transformed with three photosensitive promoters are stronger than the promoter pHhl1, and the pPhyB is the strongest.And that plants transformed with promoter pPhyB, its photosensitive efficiency is the best and its photodamage is the lowest. </div></p>
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<div align="center">
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<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/9/9f/T--GDSYZX-United--demonstrate2.png"/>
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</div>
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<p><div class="text_font">Fig.Photosynthetic efficiency in transgentic Arabidopsis</div></p>
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<table class="table" style="text-align:center">
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<thead>
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<th></th>
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<th colspan="2" style="text-align:center">Growth light</th>
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<th colspan="2" style="text-align:center">High light</th>
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</thead>
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<tbody>
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<tr>
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<td></td>
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<td>Fv/Fm</td>
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<td>SE</td>
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<td>Fv/Fm</td>
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<td>SE</td>
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</tr>
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<tr>
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<td>Phhl1</td>
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<td>0.82</td>
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<td>0.02</td>
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<td>0.65</td>
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<td>0.017</td>
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</tr>
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<tr>
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<td>Pcop1</td>
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<td>0.825</td>
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<td>0.013</td>
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<td>0.72</td>
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<td>0.0086</td>
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</tr>
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<tr>
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<td>Ppif1</td>
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<td>0.813</td>
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<td>0.006</td>
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<td>0.69</td>
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<td>0.0161</td>
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</tr>
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<tr>
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<td>PphyB</td>
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<td>0.819</td>
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<td>0.019</td>
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<td>0.78</td>
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<td>0.0216</td>
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</tr>
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</tbody>
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</table>
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Revision as of 17:59, 13 October 2016

Demonstrate

The trascription level of HHL1 in transgentic Arabidopsis

In the compete transgenic Arabidopsis，the result is very similar. The transcription controlled by pHhl1 is also very low. However, under high light, the transcription controlled by three photosensitive promoters are also very high, and the highest is phyB, and then is pCop1.

These suggested that our parts can also function in the compete plants, and the result is similar.

image

Fig.The transcription level of HHL1 in transgentic Arabidopsis

Growth light High light
REL SE REL SE
Phhl1 1 0.07 1.1 0.17
Pcop1 1 0.13 15.1 1.86
Ppif1 1 0.26 10.9 0.61
PphyB 1 0.19 20.6 1.96

Photosynthetic efficiency in transgentic Arabidopsis

We transformed four vectors with stably expressions into Arabidopsis, then detected the photosynthetic efficiency of transgenic Arabidopsis.

  1）Under growth light condition, the chloroplast of the plants that transformed in four different vectors were not damaged, so the red fluorescence of the chloroplasts have no obvious difference and maintain a high level.

  2）However, under high light condition, the construction of chloroplasts were damaged in different level although the protein HHL1 exsist. So the red fluorescence of chloroplast had decreased, but the decrease level is quit different, the lower the fluorescence is, the stronger the damage is.

From the diagram we can know that the fluorescence of plants transformed with three photosensitive promoters are stronger than the promoter pHhl1, and the pPhyB is the strongest.And that plants transformed with promoter pPhyB, its photosensitive efficiency is the best and its photodamage is the lowest.

image

Fig.Photosynthetic efficiency in transgentic Arabidopsis

Growth light High light
Fv/Fm SE Fv/Fm SE
Phhl1 0.82 0.02 0.65 0.017
Pcop1 0.825 0.013 0.72 0.0086
Ppif1 0.813 0.006 0.69 0.0161
PphyB 0.819 0.019 0.78 0.0216