Difference between revisions of "Team:BostonU/Composite Part"

Line 13: Line 13:
  
  
−
<p>
+
<br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;">
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
BBa_K1875018</p>
 +
<br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;">
 +
To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the production of a gene of interest proceeds. The genes of interest in our work were fluorescent proteins; specifically GFP was utilized in the guide RNA operator reporter vectors submitted to the registry. The guide RNA operator vector can be considered a composite part. It is constructed by combining the following parts in order: BBa_K1875009 (the target sequence), BBa_K1875000 (the miniCMV), BBa_K1875001 (the Kozak sequence), BBa_K1875003 (the GFP), BBa_K1875002 (the Rabbit Beta Globin PolyA). The sources of these parts can be found on their respective registry pages. We have submitted BBa_K1875018 for best composite part due to both its high expression and consistency in experimentation. Specifically, this part, when compared with a CMV, can express GFP at a high level than the strong promoter. This means that this part grants a scientist the ability to produce a protein at a higher level than a strong promoter and grant them a temporal control.
 +
 
 
</p>
 
</p>
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
 
−
 
−
 
−
 
−
<div class="highlight">
 
−
<h4>Note</h4>
 
−
<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
 
  
 
</div>
 
</div>

Revision as of 16:34, 14 October 2016


BBa_K1875018


To create the Gemini parts library we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the production of a gene of interest proceeds. The genes of interest in our work were fluorescent proteins; specifically GFP was utilized in the guide RNA operator reporter vectors submitted to the registry. The guide RNA operator vector can be considered a composite part. It is constructed by combining the following parts in order: BBa_K1875009 (the target sequence), BBa_K1875000 (the miniCMV), BBa_K1875001 (the Kozak sequence), BBa_K1875003 (the GFP), BBa_K1875002 (the Rabbit Beta Globin PolyA). The sources of these parts can be found on their respective registry pages. We have submitted BBa_K1875018 for best composite part due to both its high expression and consistency in experimentation. Specifically, this part, when compared with a CMV, can express GFP at a high level than the strong promoter. This means that this part grants a scientist the ability to produce a protein at a higher level than a strong promoter and grant them a temporal control.