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~~<title>iGEM Notebook · Benchling</title>~~

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<body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div><noscript></noscript><noscript></noscript><noscript></noscript></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">iGEM Notebook</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Protocols</div><div><b>Authors:</b> Kamila Drezek</div><div><b>Dates:</b> 2016-05-19 to 2016-10-09</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 5/19/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part637" data-index="0" style="margin-left:0;">Preparing Reagants</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 5/21/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part638" data-index="1" style="margin-left:35px;">LB Broth (1L)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part639" data-index="2" style="margin-left:70px;">20 grams per liter</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part640" data-index="3" style="margin-left:35px;">LB Agar (1L)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part641" data-index="4" style="margin-left:70px;">40 grams per liter</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part642" data-index="5" style="margin-left:35px;">Prepare two liter containers of each- one per antibiotic (Cam and Carb)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part643" data-index="6" style="margin-left:35px;">Autoclave for 1 hour</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part644" data-index="7" style="margin-left:0;">Preparing chloramphenicol stock (1000x)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part645" data-index="8" style="margin-left:35px;">Final concentration of 1x chloramphenicol should be 25 ug/ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part646" data-index="9" style="margin-left:35px;">Stock concentration of 1000x chloramphenicol should be 25 mg/ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part647" data-index="10" style="margin-left:35px;">Prepare 50 ml of stock to aliquot into microcentrifuge tubes by measuring 1.25 g of chloramphenicol and ~50ml of 200 proof ethanol in a 50 ml Falcon </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part648" data-index="11" style="margin-left:35px;">Filter chloramphenicol through 20 um syringe filter</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part649" data-index="12" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part650" data-index="13" style="margin-left:0;">Preparing Plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part651" data-index="14" style="margin-left:35px;">20ml agar per plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part652" data-index="15" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part653" data-index="16" style="margin-left:0;">Innoculate Vector 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part654" data-index="17" style="margin-left:35px;">5 ml of LB+carb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part655" data-index="18" style="margin-left:35px;">Stab cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part656" data-index="19" style="margin-left:35px;">Grow overnight at 37C shaker</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 5/22/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part657" data-index="20" style="margin-left:35px;">Preparing Cell stock of 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part658" data-index="21" style="margin-left:70px;">Box Name- iGEM 2016 Source Box</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part659" data-index="22" style="margin-left:70px;">Freezer Number- 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part660" data-index="23" style="margin-left:70px;">Shelf- 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part661" data-index="24" style="margin-left:35px;">Vector 1721 is miniprepped</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part662" data-index="25" style="margin-left:70px;">See protocol sheet</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part663" data-index="26" style="margin-left:35px;">Vector 1721 is digested (300ul digest)(1-2hrs)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part664" data-index="27" style="margin-left:70px;">24000 ng digestion of DNA </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part665" data-index="28" style="margin-left:105px;">30 ul buffer (cold room)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part666" data-index="29" style="margin-left:105px;">9ul of BpsI (-80C, small green box)(incubation in 37C)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part667" data-index="30" style="margin-left:105px;">101 ul of water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part668" data-index="31" style="margin-left:35px;">Digestion Clean-Up of Vector 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part669" data-index="32" style="margin-left:70px;">See PCR cleanup protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part670" data-index="33" style="margin-left:70px;">Note- vector was eluted in 50 ul of EB not 30 ul. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part671" data-index="34" style="margin-left:35px;">Dilution of Oligos (ideal final concentration is 100uM)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part672" data-index="35" style="margin-left:70px;">Short Cut- </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part673" data-index="36" style="margin-left:105px;">300 ul of TE for values ~25- ~35 nmol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part674" data-index="37" style="margin-left:105px;">200 ul of TE for value ~15 - ~24 nmol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part675" data-index="38" style="margin-left:70px;">Note- Oligo 12, 13, 14, 17, 18, and 20 was vortex without liquid initially</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part676" data-index="39" style="margin-left:35px;">Annealing of pGEX oligos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part677" data-index="40" style="margin-left:70px;">See protocol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part678" data-index="41" style="margin-left:70px;">Annealing time- 2 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part679" data-index="42" style="margin-left:70px;">dilute oligos to 1ng/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part680" data-index="43" style="margin-left:35px;">Ligation of 1721 and insert</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part681" data-index="44" style="margin-left:70px;">Note- diluted annealed oligos named- pGEX#A where # is the number of the construct</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part682" data-index="45" style="margin-left:70px;">24 reactions</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part683" data-index="46" style="margin-left:105px;">139.2 ul of water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part684" data-index="47" style="margin-left:105px;">24 ul of T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part685" data-index="48" style="margin-left:105px;">24 ul of T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part686" data-index="49" style="margin-left:105px;">26.4 ul of Vector</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part687" data-index="50" style="margin-left:35px;">Transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part688" data-index="51" style="margin-left:70px;">See protocol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part689" data-index="52" style="margin-left:70px;">Plates put in incubator at 7:09 PM- Desired pick up time is 10:09</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 5/23/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part690" data-index="53" style="margin-left:70px;">pGEX plates were pulled from incubator at 10:15</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part691" data-index="54" style="margin-left:105px;">Growth and density of all samples exceeded that of the negative control</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 5/24/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part692" data-index="55" style="margin-left:70px;">Picked two colonies per plate and grew in 4ml of LB+carb in 37C shaker. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part693" data-index="56" style="margin-left:70px;">Created stab plate for each colony. </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 5/25/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part694" data-index="57" style="margin-left:70px;">Minipreping colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part695" data-index="58" style="margin-left:105px;">Notes: During the miniprep samples from colony 10 were contaminated</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part696" data-index="59" style="margin-left:70px;">Test Cut </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part697" data-index="60" style="margin-left:105px;">4 ul DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part698" data-index="61" style="margin-left:105px;">1 ul 2.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part699" data-index="62" style="margin-left:105px;">.25 of ul each enzyme (Bsp1 and Nco1)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part700" data-index="63" style="margin-left:105px;">4.5 ul water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part701" data-index="64" style="margin-left:105px;">Run digestion for at least 15 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part702" data-index="65" style="margin-left:70px;">Test Cut Results</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part703" data-index="66" style="margin-left:105px;">All colonies appeared with proper bands at 1.1 kb and 4.2kb and no bands at 440 bp. We decided to send only the first colony from each set in for sequencing</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part704-item" data-formattable="true" data-index="67" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">pGEX1-1 to pGEX12-2 test cuts</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="XZPsOM1Dwmy0jxyx52av0qGrK7hOK5q9mzmggCU7-IMG_8763.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part704" data-index="67">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part706-item" data-formattable="true" data-index="68" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">pGEX13-1 to pGEX20-2 test cuts</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="SOkSYjGFUBFO3eDx6yVdJNsaCyR1fCluH49hD24N-IMG_8764.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part706" data-index="68">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 5/26/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part708" data-index="69" style="margin-left:35px;">Sequencing results are in</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part709" data-index="70" style="margin-left:70px;">All sequences with the exceptions of pGEX1-1, pGEX14-1, and pGEX 15-1 were successful.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part710" data-index="71" style="margin-left:105px;">All sequences reamining sequences were then grown up for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part711" data-index="72" style="margin-left:140px;">Note: While pGEX 6-1 sequenced properly the tube containing its reference appeared to have been contaminated and pGEX 6-2 will be sent in for sequencing to reverify</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part712" data-index="73" style="margin-left:35px;">Sequencing sent in</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part713" data-index="74" style="margin-left:70px;">pGEX: 1-2, 6-2, 14-1, 14-2, 15-1, 15-2, 10-1, 10-2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part714" data-index="75" style="margin-left:35px;">Restriction digest of 2490 and DC16 with MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part715" data-index="76" style="margin-left:70px;">5ul buffer3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part716" data-index="77" style="margin-left:70px;">6ul DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part717" data-index="78" style="margin-left:70px;">1.5ul Nru1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part718" data-index="79" style="margin-left:70px;">1.5ul Mlu1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part719" data-index="80" style="margin-left:70px;">36ul water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part720" data-index="81" style="margin-left:70px;">Prerequisites to subvectors which will be used to create one plasmid with Cre and both Flps </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part721" data-index="82" style="margin-left:105px;">Subvectors to be made have homology with each other, allowing for easy gibson assembly with no PCR (promoters and ERT2 - modified estrogen receptor - have repeat regions)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part722" data-index="83" style="margin-left:70px;">Digestion of DC16 yielded 3 bands rather than the expected 2. Culprit may be unreliable cell stock.</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part723-item" data-formattable="true" data-index="84" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">DC16 2490 1st digestion.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JghBH5DpmwrD1qFjKyUVXR7yoGrbaeBjMa8Xmo5F-DC16_2490_1st_digestion.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:~~0;"><~~/div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part723" data-index="84">We were supposed to have: DC16 bands at 10723bp,<b><u> 1172bb</~~u></b>~~. ~~2490 bands at <b><u>10590bp</u></b> and 227bp~~.

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part725" data-index="85" style="margin-left:35px;">Ligate 2490 and DC16 to create 2534 (10ul)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part726" data-index="86" style="margin-left:70px;">See Ligation Calculator</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part727" data-index="87" style="margin-left:70px;">4.2 ul DI water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part728" data-index="88" style="margin-left:70px;">1.0 ul DC16 (insert)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part729" data-index="89" style="margin-left:70px;">2.8 ul 2490 (vector)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part730" data-index="90" style="margin-left:70px;">1 ul T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part731" data-index="91" style="margin-left:70px;">1 ul T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part732" data-index="92" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part733" data-index="93" style="margin-left:35px;">Construct 2534 and a negative control are transformed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part734" data-index="94" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part735" data-index="95" style="margin-left:35px;">Tissue Culture Room</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part736" data-index="96" style="margin-left:70px;">Prepping hood for trannsfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part737" data-index="97" style="margin-left:105px;">Remember to clean the hood down with 70% ethanol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part738" data-index="98" style="margin-left:70px;">Aspirate all media from the plate to aid in the trypsin function</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part739" data-index="99" style="margin-left:105px;">Kidney cells are stored in T75 flask</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part740" data-index="100" style="margin-left:70px;">After aspiration perform a PBS wash</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part741" data-index="101" style="margin-left:105px;">Phosphate Buffer Solution- a salt water solution (~5ml wash)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part742" data-index="102" style="margin-left:70px;">Trypsin is used remove kidney cells from the plate.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part743" data-index="103" style="margin-left:105px;">Add 2.5ml of Trypsin to the T75</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part744" data-index="104" style="margin-left:140px;">Dont shoot directed at the cells, let the trpysin run over them.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part745" data-index="105" style="margin-left:70px;">Addition of Medium will neutralize trypsin</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part746" data-index="106" style="margin-left:105px;">Add 7.5 ml of media to plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part747" data-index="107" style="margin-left:105px;">And tritrate (Pipette up and down)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part748" data-index="108" style="margin-left:105px;">To improve cell count- spin cells down then respend them</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part749" data-index="109" style="margin-left:140px;">Spin at 300 RCF for 2-5 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part750" data-index="110" style="margin-left:140px;">To resuspend- tap the hood with the 50ml falcon tube</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part751" data-index="111" style="margin-left:140px;">Add back 10 ml of media and tritrate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part752" data-index="112" style="margin-left:70px;">Performing a cell count</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part753" data-index="113" style="margin-left:105px;">Add 10 ul of cell solution to a cell counting slides</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part754" data-index="114" style="margin-left:140px;">Divide the cell count concentration by 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part755" data-index="115" style="margin-left:140px;">Actual Cell Counts</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:175px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part756" data-index="116" style="margin-left:175px;">~2.5 Million cells per ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part757" data-index="117" style="margin-left:70px;">Magic Number for transfection:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part758" data-index="118" style="margin-left:105px;">200,000 cells per ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part759" data-index="119" style="margin-left:105px;">For each T75- 20 ml of solution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part760" data-index="120" style="margin-left:105px;">For desired concentration of cells- </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part761" data-index="121" style="margin-left:140px;">Add 4.8 ml of cell concentrate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part762" data-index="122" style="margin-left:140px;">Add 55.2 ml of media</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part763" data-index="123" style="margin-left:105px;">Continuing the cell line</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part764" data-index="124" style="margin-left:140px;">Note: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:175px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part765" data-index="125" style="margin-left:175px;">Split/Passage: diluting cells in the same vessel </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part766" data-index="126" style="margin-left:140px;">Split the parent cell line </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 5/27/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part767" data-index="127" style="margin-left:35px;">pGEX Cells moved to Stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part768" data-index="128" style="margin-left:70px;">1-13, 16-20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part769" data-index="129" style="margin-left:35px;">Sequencing results </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part770" data-index="130" style="margin-left:70px;">6-2 and 1-2 are perfect</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part771" data-index="131" style="margin-left:70px;">pGEX 14 ans 15 failde to sequence again</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part772" data-index="132" style="margin-left:105px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part773" data-index="133" style="margin-left:35px;">Transformation for Construct 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part774" data-index="134" style="margin-left:70px;">No significant growth in the positive- less than what appeared in the negative -> failure</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part775" data-index="135" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part776" data-index="136" style="margin-left:35px;">Digest a different aliquot of DC16 at a low concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part777" data-index="137" style="margin-left:70px;">Need 4ng of DNA with DNA at 45ng/uL so need 89 uL of DNA; will do 100 ish uL digest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part778" data-index="138" style="margin-left:70px;">89 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part779" data-index="139" style="margin-left:70px;">1.5 uL MluI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part780" data-index="140" style="margin-left:70px;">1.5 uL NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part781" data-index="141" style="margin-left:70px;">10 uL Buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part782" data-index="142" style="margin-left:70px;">No water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part783" data-index="143" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part784" data-index="144" style="margin-left:35px;">Ligation of dc16 insert and 2490 vector</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part785" data-index="145" style="margin-left:70px;">2.7 ul of vector</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part786" data-index="146" style="margin-left:70px;">1.7 ul of insert</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part787" data-index="147" style="margin-left:70px;">3.5 ul of water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part788" data-index="148" style="margin-left:70px;">1 ul of T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part789" data-index="149" style="margin-left:70px;">1 ul of T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part790" data-index="150" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part791" data-index="151" style="margin-left:35px;">Digestion of pSb1A3 and pBS1C3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part792" data-index="152" style="margin-left:70px;">40 ul of DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part793" data-index="153" style="margin-left:70px;">5 ul of Cutsmart BUffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part794" data-index="154" style="margin-left:70px;">1.5 ul of EcoRI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part795" data-index="155" style="margin-left:70px;">1.5 ul of PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part796" data-index="156" style="margin-left:70px;">2 ul of Water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part797" data-index="157" style="margin-left:70px;">After digestion treat witl DnpI for 15 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part798" data-index="158" style="margin-left:70px;">PCR Clean- up and elute in 30 ul of EB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part799" data-index="159" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part800" data-index="160" style="margin-left:35px;">Gibson of pSb1A3 (2533) and pSb1C3 (2531)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part801" data-index="161" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part802" data-index="162" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">CONSTRUCT </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">NAME</span></span><span style="color:#344043"><span style="background-color:#FFFFFF"> </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">2531</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part803" data-index="163" style="margin-left:0;">Mass Vec =25

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</~~div><~~/div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part804" data-index="164" style="margin-left:0;">Ratio = 3

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+

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</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part805" data-index="165" style="margin-left:0;"> SIZE 2000 1200 600 0 0

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</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part806" data-index="166" style="margin-left:0;"> CONC 5.6 10 10 1 1

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</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part807" data-index="167" style="margin-left:0;"> VOL 4.5 4.5 2.3 0.0 0.0 -1.2

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</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part808" data-index="168" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">CONSTRUCT </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">NAME </span></span>2533

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</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part809" data-index="169" style="margin-left:0;"> SIZE 2000 1200 600 0 0

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</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part810" data-index="170" style="margin-left:0;"> CONC 23 10 10 1 1

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</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part811" data-index="171" style="margin-left:0;"> VOL 1.1 4.5 2.3 0.0 0.0 2.2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part812" data-index="172" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part813" data-index="173" style="margin-left:35px;">Both the Gibson Assembly Transformation and the Ligation Transformation are over 10kb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part814" data-index="174" style="margin-left:70px;">See Protocol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part815" data-index="175" style="margin-left:0;">Stable Line Integration of 2494-2496</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part816" data-index="176" style="margin-left:35px;">See Protocol for Stable Line Transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part817" data-index="177" style="margin-left:35px;">Construct 900- Piggy back transpose</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part818" data-index="178" style="margin-left:35px;">Cell status preceeding transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part819" data-index="179" style="margin-left:70px;">Low cell count ~30-40% confluences </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part820" data-index="180" style="margin-left:35px;">Thaw out 2 tubes of PEI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part821" data-index="181" style="margin-left:70px;">Use an incubator in a BL2 room</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part822" data-index="182" style="margin-left:35px;">Note: We are making 4x the number of cells than what is required</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part823" data-index="183" style="margin-left:35px;">Mixes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part824" data-index="184" style="margin-left:70px;">DNA part</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part825" data-index="185" style="margin-left:70px;">.78 ul of transposase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part826" data-index="186" style="margin-left:70px;">3.2 ul of DNA </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part827" data-index="187" style="margin-left:70px;">.15 M NaCl (3ml per tube)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part828" data-index="188" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part829" data-index="189" style="margin-left:70px;">PEI part</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part830" data-index="190" style="margin-left:70px;">2ml of PEI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part831" data-index="191" style="margin-left:70px;">.15 M NaCl ( 10.5 ml)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part832" data-index="192" style="margin-left:35px;">Add 4 ml of PEI part to DNA part</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part833" data-index="193" style="margin-left:35px;">Transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part834" data-index="194" style="margin-left:70px;">Don't pour directly onto the- shoot away from the cells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 5/28/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part835" data-index="195" style="margin-left:35px;">Create cell stock of pGEX10-1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part836" data-index="196" style="margin-left:35px;">MIniprep of 14-3/4/5 15-3/4/5 and 2534-1/2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part837" data-index="197" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part838" data-index="198" style="margin-left:35px;">Test Cut Results</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part839-item" data-formattable="true" data-index="199" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05-26-16 pGEX14,15 and 2434 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vFOrZaGyaRhiPRTWzSMBGXdbdGvuxYHrqKdf5XTk-05-26-16_pGEX1415_and_2434_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part839" data-index="199">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part841" data-index="200" style="margin-left:35px;">pGEX 14-3, pGEX 15-5, 2534-2 => sent to sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part842" data-index="201" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part843" data-index="202" style="margin-left:35px;">Cell inspection preceeding puromycin selection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part844" data-index="203" style="margin-left:70px;">~90% confluence</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part845" data-index="204" style="margin-left:35px;">Puromycin Selection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part846" data-index="205" style="margin-left:70px;">4ul of concentrated puromycin - 2ug per ml to each flask</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part847" data-index="206" style="margin-left:35px;">Wait 10 days for total transient plasmids are diluted outs.</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 5/29/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part848" data-index="207" style="margin-left:0;">Sequencing Results</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part849" data-index="208" style="margin-left:35px;">pGEX 15-5 can be moved to cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part850" data-index="209" style="margin-left:35px;">pGEX 14-3 failed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part851" data-index="210" style="margin-left:35px;">2534 failed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part852" data-index="211" style="margin-left:0;">Sent Sequencing </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part853" data-index="212" style="margin-left:35px;">pGEX 14-4, pGEX 14-5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part854" data-index="213" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part855" data-index="214" style="margin-left:0;">Digestion</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part856" data-index="215" style="margin-left:35px;">2531 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part857" data-index="216" style="margin-left:70px;">4 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part858" data-index="217" style="margin-left:70px;">4 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part859" data-index="218" style="margin-left:70px;">1 uL CutSmart</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part860" data-index="219" style="margin-left:70px;">0.5 uL EcoRI and AgeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part861" data-index="220" style="margin-left:35px;">2533</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part862" data-index="221" style="margin-left:70px;">4 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part863" data-index="222" style="margin-left:70px;">4 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part864" data-index="223" style="margin-left:70px;">1 uL CutSmart</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part865" data-index="224" style="margin-left:70px;">0.5 uL EcoRI and AgeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part866" data-index="225" style="margin-left:35px;">DC16 midiprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part867" data-index="226" style="margin-left:70px;">1 uL DNA at 1:10 dilution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part868" data-index="227" style="margin-left:70px;">7 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part869" data-index="228" style="margin-left:70px;">1 uL Buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part870" data-index="229" style="margin-left:70px;">0.5 uL MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part871" data-index="230" style="margin-left:35px;">DC16 miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part872" data-index="231" style="margin-left:70px;">2 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part873" data-index="232" style="margin-left:70px;">6 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part874" data-index="233" style="margin-left:70px;">1 uL Buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part875" data-index="234" style="margin-left:70px;"><span style="color:#344043"><span style="background-color:#FFFFFF">0.5 uL MluI and NruI</span></span></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part876-item" data-formattable="true" data-index="235" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05-27-2016 DC16 DC56 2531 test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="8U0ZRkGS72jLVot8K8KxyFpZfIuTISwDvtnvYD8O-05-27-2016_DC16_DC56_2531_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part876" data-index="235">

+

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part878" data-index="236" style="margin-left:0;">dc16 Primers</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part879" data-index="237" style="margin-left:0;">BP0165, BP0162 reverse</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part880" data-index="238" style="margin-left:0;">BP0198, BP0176 forward</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part881" data-index="239" style="margin-left:0;">dc56 Primers</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part882" data-index="240" style="margin-left:0;">BP0165, BP0130 reverse</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part883" data-index="241" style="margin-left:0;">BP0038, BP0188 forward</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part884" data-index="242" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part885" data-index="243" style="margin-left:0;">pGEX 14-4 and pGEX14-5 moved to cell stock pending sequencing</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 5/31/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part886" data-index="244" style="margin-left:0;">Phosphorylating and Annealing Oligos for pGOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part887" data-index="245" style="margin-left:0;">Moved Construct 2531 to cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part888" data-index="246" style="margin-left:0;">Grow up more 2531 for ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part889" data-index="247" style="margin-left:35px;">Because of Chloramphenicol's effect on growth, we will grow this up for 24 hours</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part890" data-index="248" style="margin-left:0;">Oligos are at 1000x concentration- dilute to 1x (~1ng/ul)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part891" data-index="249" style="margin-left:0;">Picked more colonies from 2533 and grew up overnight</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/1/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part892" data-index="250" style="margin-left:0;">Miniprep 2531 (grown again due to low inital concentration) and 2533 (initial sequencing was incorrect)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part893" data-index="251" style="margin-left:0;">Digest 2531 with BbsI (50 uL total)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part894" data-index="252" style="margin-left:35px;">30 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part895" data-index="253" style="margin-left:35px;">5 uL Buffer 2.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part896" data-index="254" style="margin-left:35px;">2 uL BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part897" data-index="255" style="margin-left:35px;">13 uL DI water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part898" data-index="256" style="margin-left:0;">Ligate oligos with 2531 to create pGOP1-20 (10uL total)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part899" data-index="257" style="margin-left:35px;">See protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part900" data-index="258" style="margin-left:35px;">0.4 uL 2531 (BbsI)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part901" data-index="259" style="margin-left:35px;">1.3 uL annealed oligos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part902" data-index="260" style="margin-left:35px;">6.4 uL DI water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part903" data-index="261" style="margin-left:35px;">1 uL T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part904" data-index="262" style="margin-left:35px;">1 uL T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part905" data-index="263" style="margin-left:0;">All pGOP plasmids were transformed and plated (25 ul) with Control</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part906" data-index="264" style="margin-left:0;">Test Cut 2533 with EcoR1 Age1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part907-item" data-formattable="true" data-index="265" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-01-2016 testcut of 2533.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ceUPYfY3mt1dz4j7IeklYEn5SmLdHRWXhKPNfqPc-06-01-2016_testcut_of_2533.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part907" data-index="265">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part909" data-index="266" style="margin-left:0;">2533-3/4/5 were sent in for sequencing </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part910" data-index="267" style="margin-left:0;">Prepped for creation of comp cells for tomorrow</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part911" data-index="268" style="margin-left:35px;">Mix 25g PEG8000 with 30ml water and disolve over night</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/2/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part912" data-index="269" style="margin-left:0;">PIck 2 colonies for each pGOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part913" data-index="270" style="margin-left:0;">Miniprepped dc16 and dc56</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part914" data-index="271" style="margin-left:0;">Prepared 200ml TSS Solution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part915" data-index="272" style="margin-left:0;">dc 16 test cut with MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part916" data-index="273" style="margin-left:35px;">expected bands: 10723, 1172</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part917-item" data-formattable="true" data-index="274" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-02-2016 ~~DC16 testcuts.jpeg<~~/div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="AhIlY1fhkvZb7LGvV0f3o7kj80dlReRldAsa8DnD-06-02-2016_DC16_testcuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part917" data-index="274">DC16: 1,2,3,5 were good

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part919" data-index="275" style="margin-left:0;">dc 56 test cut with AgeIHF and EcoRIHF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part920" data-index="276" style="margin-left:35px;">expected bands: 6700, 1000</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part921" data-index="277" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part922" data-index="278" style="margin-left:0;">Primer 0170 for 2533 failed and this impeded the sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part923" data-index="279" style="margin-left:0;">Preparing Top10 comp cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part924" data-index="280" style="margin-left:35px;">First OD (optical density) measurement: 0.2 (time: 2:15 p.m.)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part925" data-index="281" style="margin-left:35px;">Second OD measurement: 0.34 (time 2:45 p.m.)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part926" data-index="282" style="margin-left:35px;">Third OD measurement: 0.419 (time 3:05 p.m.)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part927" data-index="283" style="margin-left:35px;">Fourth OD measurment: 0.486 (time 3:35 p.m.)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/3/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part928" data-index="284" style="margin-left:0;">miniprepped pGOP plasmids</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part929" data-index="285" style="margin-left:0;">test cut pGOP plasmids with Bbs1 and Spe1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part930-item" data-formattable="true" data-index="286" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-03-2016 pGOP testcuts 1-8.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="6mjyQMxVd4Ynr7gWkVn3P5VAuoMxQoNzHeYexVqO-06-03-2016_pGOP_testcuts_1-8.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part930" data-index="286">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part932-item" data-formattable="true" data-index="287" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-03-2016 pGOP9-20 testcuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="L9GS5uo2EU9ddMPkijaoSraeewfdM9H8F72NBIHS-06-03-2016_pGOP9-20_testcuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part932" data-index="287">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part934" data-index="288" style="margin-left:0;">grew up more _____ for midiprep</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 6/4/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part935" data-index="289" style="margin-left:0;">Picked three colonies of pGOP 20 (3, 4, and 5)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part936" data-index="290" style="margin-left:0;">Grew up pGOP 20-3/4/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part937" data-index="291" style="margin-left:0;">Miniprep 2533-7/8/~~9</div></div></div><div class="daySeparator"><div class="daySeparator~~-~~inputWrapper"><input class="daySeparator~~-~~inputWrapper~~-~~hiddenInput" type="input"/></div><span class="daySeparator~~-date">Sunday, 6/5/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part938" data-index="292" style="margin-left:0;">Miniprep 20-3/4/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part939" data-index="293" style="margin-left:0;">Test Cut for 2533 and pGOP 20 addition colonies</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part940-item" data-formattable="true" data-index="294" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-05-2016 pGOP20 and 2533 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="raEniQFACMZ6WTWFCcHbcTjZDHj2n1sPDRniOu4f-06-05-2016_pGOP20_and_2533_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part940" data-index="294">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/6/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part942" data-index="295" style="margin-left:0;">Miniprep pGOP20-R1/R2/R3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part943" data-index="296" style="margin-left:0;">Test cuts for pGOP20-R1/R2/R3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part944" data-index="297" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part945" data-index="298" style="margin-left:0;">Cell Stock pGOP 1-19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part946" data-index="299" style="margin-left:0;">Grew up DC16-1 and DC56-1 for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part947" data-index="300" style="margin-left:0;">Sent pGOP 20-3/4/5 and 2533-7/8/9 for sequencing </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part948" data-index="301" style="margin-left:0;">Diluted pGEX 1-20 and pGOP 1-19 to 50 ng/uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part949" data-index="302" style="margin-left:0;">Made a liter of LB + Carb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part950" data-index="303" style="margin-left:0;">Nanodropped pGEX 1-20 and pGOP 1-19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part951" data-index="304" style="margin-left:35px;">Inoculated pGEX16-1 due to low concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part952" data-index="305" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part953" data-index="306" style="margin-left:0;">Midiprep for Transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part954" data-index="307" style="margin-left:35px;">Prepared 150 mL of LB + Carb with pCJH2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part955" data-index="308" style="margin-left:35px;">Prepared 150 mL of LB + Carb with T40</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part956" data-index="309" style="margin-left:35px;">Prepared 150 mL of LB + Carb with pBW363</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/7/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part957" data-index="310" style="margin-left:0;">Plan for transfection of pGEXs and pGOPs to be performed on Thursday</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part958-item" data-formattable="true" data-index="311" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">new doc 7_1.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="RiqKnvcxp4977zdKMNwMbEj5tJeV85CDRWS2Ucyn-new_doc_7_1.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part958" data-index="311">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part960" data-index="312" style="margin-left:0;">Miniprep of pGOP12, pGEX16, DC174 and DC100 (both DCs will be used as positive controls transfection on Thursday)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part961" data-index="313" style="margin-left:0;">Midiprep of pBW363 (Blank), T40 (dCas9), pCJH2 (BFP transfection marker), DC16 and DC56</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part962" data-index="314" style="margin-left:35px;">Evaporated supernatant</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/8/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part963" data-index="315" style="margin-left:0;">Cell stocked pGOP 20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part964" data-index="316" style="margin-left:0;">50ul Restriction digest of 2490 and DC16 with MluI and NruI to create 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part965" data-index="317" style="margin-left:35px;">5ul buffer3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part966" data-index="318" style="margin-left:35px;">6ul DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part967" data-index="319" style="margin-left:35px;">1.5ul Nru1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part968" data-index="320" style="margin-left:35px;">1.5ul Mlu1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part969" data-index="321" style="margin-left:35px;">36ul water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part970" data-index="322" style="margin-left:35px;">incubate 1 hr in 37C</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part971" data-index="323" style="margin-left:35px;">Got 3 bands so threw out digest and are now investigating our enzymes to see if there has been contamination</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part972" data-index="324" style="margin-left:0;">Stocked midiprep DNA with 1x TE</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part973" data-index="325" style="margin-left:0;">Grew up T40 for further minipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part974" data-index="326" style="margin-left:0;">Prepared HEK293 plates for tomorrow's transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part975" data-index="327" style="margin-left:35px;">Add 250ul of 200000cells/ml cell solution to each well on 48 well plate</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/9/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part976" data-index="328" style="margin-left:0;">Transfected HEK cells</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part977-item" data-formattable="true" data-index="329" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.18.39.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JdPPlxzJdcUw397LN76mBuwsX1rODIWWWBP84d2D-Screen_Shot_2016-08-01_at_12.18.39.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part977" data-index="329">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part979" data-index="330" style="margin-left:0;">Miniprepped T40</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part980" data-index="331" style="margin-left:0;">Diluted pCJH2, pBW363, and T40 for transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part981" data-index="332" style="margin-left:0;">Checked and rediluted pGEXs and pGOPs for transfection </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part982" data-index="333" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part983" data-index="334" style="margin-left:0;">note- pGEX11 is contaminated</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part984" data-index="335" style="margin-left:35px;">pGEX7 is too low of concentration</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/10/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part985" data-index="336" style="margin-left:0;">Checked transfection plates under a microscope</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part986" data-index="337" style="margin-left:35px;">all worked; however, Cre showed leakiness</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part987" data-index="338" style="margin-left:0;">Resent DC16-1 for sequencing due to QuintaraBio sequencing faillure</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part988" data-index="339" style="margin-left:0;">Passaging HEK293 cells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part989" data-index="340" style="margin-left:0;">Minipreppred pGOP1-20 (inoculated in Cam last night)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part990" data-index="341" style="margin-left:0;">Cut DC16 and 2490 with MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part991" data-index="342" style="margin-left:35px;">8 uL DC16 or 11uL of 2490</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part992" data-index="343" style="margin-left:35px;">1.5 uL MluI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part993" data-index="344" style="margin-left:35px;">1.5 uL NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part994" data-index="345" style="margin-left:35px;">5uL buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part995" data-index="346" style="margin-left:35px;">34 uL water or 31 uL water (2490)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part996" data-index="347" style="margin-left:0;">Run Gel Extraction on 2490 and DC16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part997" data-index="348" style="margin-left:35px;">Bands</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part998" data-index="349" style="margin-left:35px;">DC16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part999" data-index="350" style="margin-left:70px;">10723</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1000" data-index="351" style="margin-left:70px;">1172 - WANTED BAND</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1001" data-index="352" style="margin-left:35px;">2490</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1002" data-index="353" style="margin-left:70px;">10590 - WANTED BAND</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1003" data-index="354" style="margin-left:70px;">227</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1004-item" data-formattable="true" data-index="355" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-13-2016 dc16 2490.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tDdhcbr26BboQeWYaen04z07LQTpQYcO9pRcHrLE-06-13-2016_dc16_2490.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1004" data-index="355">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1006" data-index="356" style="margin-left:0;">Ligation of 2490 and DC16 to make 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1007" data-index="357" style="margin-left:0;">Ligations were transformed and plated</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1008" data-index="358" style="margin-left:35px;">Diluted primers for PCR reaction of pGPX1 (2531)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1009" data-index="359" style="margin-left:35px;">PCR GFP, BFP and mRuby </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1010" data-index="360" style="margin-left:35px;">Digested pGOPs 3,5,6,8,10,14,15,19 and pGPX1 with Mlu1-HF and Age1-HF in preparation of making pGOPs with GFP, BFP and mRuby</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1011-item" data-formattable="true" data-index="361" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-14-2016 pGOPs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="YnlkemaP7ktXgp5wOg6pUUHEjISRsU7uD4BHAeAL-06-14-2016_pGOPs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1011" data-index="361">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1013" data-index="362" style="margin-left:35px;">Miniprep 2531 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1014" data-index="363" style="margin-left:35px;">Picked colonies for 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1015" data-index="364" style="margin-left:35px;">Transfected HEK cells with different concentrations of the 8 pGOPs given above, to test whether the concentration of pGOPs in the cell was causing high levels of basal expression)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1016" data-index="365" style="margin-left:70px;">Transfected transfection marker, T40 (dCas9) pGEXs, Blank plasmid and pGOP. pGOP at 62.5, 30 and 7.5 ng</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1017-item" data-formattable="true" data-index="366" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.17.24.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="xDW7aNbCjJtaDjqaCiMDH4UfL7xkmUKOW6WBWN3d-Screen_Shot_2016-08-01_at_12.17.24.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1017" data-index="366">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1019" data-index="367" style="margin-left:35px;">PCR digested GFP, BFP and mRuby with Mlu1-HF and Age1-HF</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1020-item" data-formattable="true" data-index="368" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-14-2016 Primers.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="9pc21ujJX4sFZ4ZCwdIVJ7aAAY4GW9fyQXTjuUW7-06-14-2016_Primers.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1020" data-index="368">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1022" data-index="369" style="margin-left:0;">Minipreppred T40 (was running low)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1023" data-index="370" style="margin-left:0;">Ligated GFP, BFP and mRuby into pGOP 3,5,6,8,10,14,15,19. Transformed onto Cam plates</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1024" data-index="371" style="margin-left:0;">Ran FACs on transfection from Tuesday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1025" data-index="372" style="margin-left:0;">PCR the silencer out of DC100 (has UAS and pG5) to put into pGOPs and pGPX6 with PrA4 and PrA4r</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1026" data-index="373" style="margin-left:0;">Digested DC 100 and prepared annealed oligos o83-o122 to prepare _________</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1027" data-index="374" style="margin-left:0;">Miniprepped Transformed plasmids from 6-15-16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1028" data-index="375" style="margin-left:0;">PCR for GFP, BFP, and mRuby</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1029-item" data-formattable="true" data-index="376" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 GFP BFP mRuby.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="NBesFu9vyPTZJoK86NXj54vBTySURGy7BYIK1ceL-06-17-2016_GFP_BFP_mRuby.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1029" data-index="376">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1031-item" data-formattable="true" data-index="377" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 2534t and 2534b.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="eLwiuU57MQk4xygqtqf34Nyzxj2uwPIKClzUakSE-06-17-2016_2534t_and_2534b.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1031" data-index="377">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1033" data-index="378" style="margin-left:0;">Sent plasmdis in for sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1034" data-index="379" style="margin-left:0;">Sequence for 2534B-4 is verified as functioning and will be moved forward with cloning</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1035-item" data-formattable="true" data-index="380" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 bfp gfp pgops.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="HvHftjVaS7ph0FZAsDnmu2QjcEc92n9IsEg9ozAN-06-17-2016_bfp_gfp_pgops.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1035" data-index="380">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1037-item" data-formattable="true" data-index="381" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 mRuby pGOPs and 2531.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="oRzmTDYjiOUva7UjQp3f6Yq7vdwa9cSCFHARGNDe-06-17-2016_mRuby_pGOPs_and_2531.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1037" data-index="381">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1039" data-index="382" style="margin-left:0;">Cell Stocked plasmids for BFP, GFP, and mRuby that sequenced properly</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1040" data-index="383" style="margin-left:0;">Miniprepped plasmids</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1041" data-index="384" style="margin-left:0;">Picked colonies for mRuby pGOP 10,14, and 15</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1042" data-index="385" style="margin-left:0;">Cell stocked BFP pGOP 15</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1043" data-index="386" style="margin-left:0;">Picked colonies for GFP pGOP 8, GFP pGOP 19, mRuby pGOP 10, mRuby pGOP 15, and mRuby pGOP 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1044" data-index="387" style="margin-left:0;">Miniprepped plasminds from 6-20-16 (mRuby pGOP 10-3, 10-4, 14-3, 14-5, 14-6, 15-3)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1045" data-index="388" style="margin-left:0;">Sent mRuby pGOP 14-3/4/5 and BFP pGOP 19-2 for sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1046" data-index="389" style="margin-left:0;">Passaged new HEK cells to P7</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1047" data-index="390" style="margin-left:0;">Made 2 new bottles of D5 Media (D5= 5%~~FBS)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px~~;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1048" data-index="391" style="margin-left:35px;">500ml of DMEM base</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1049" data-index="392" style="margin-left:35px;">25ml FBS (alloquoted in fridge across hallway from TC room)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1050" data-index="393" style="margin-left:35px;">5ml L-Glut</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1051" data-index="394" style="margin-left:35px;">5ml P/S</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1052" data-index="395" style="margin-left:35px;">5ml sodium pyruvate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1053" data-index="396" style="margin-left:35px;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1054" data-index="397" style="margin-left:0;">Cell stocked picked colonies from 6/21/16 (<span style="color:#344043"><span style="background-color:#FFFFFF">GFP pGOP 8, GFP pGOP 19, mRuby pGOP 10, mRuby pGOP 15, and mRuby pGOP 19</span></span>).</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1055" data-index="398" style="margin-left:0;">Gel extract PrA4/4r (silencer) </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1056-item" data-formattable="true" data-index="399" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-23-2016 PCR of silencer.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="G8BxvwPcEI2hVwW8qzJh97BJhe9BUSCoFT3krwyb-06-23-2016_PCR_of_silencer.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1056" data-index="399">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1058" data-index="400" style="margin-left:0;">Digested DC100 with NheI and dropped in annealed oligos with ligation to make pDOP 1-20 (backbone from DC100 with gRNAs dropped in)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1059" data-index="401" style="margin-left:0;">sent pGOP 6-9 back in for sequencing - suspected error in gRNA target site</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1060" data-index="402" style="margin-left:0;">ligate and transform pDOP 1-20 (DC100 and annealed oligos)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1061" data-index="403" style="margin-left:0;">inoculate pGPX1 so we can make pGPX2 and 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1062" data-index="404" style="margin-left:0;">Prepped for transfextion</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1063" data-index="405" style="margin-left:0;">Picked pDOP1-20 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1064" data-index="406" style="margin-left:0;">Gel extracted pGPX1</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1065" data-index="407" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">ligate and transfrom pGPX2 and pGPX3 (pGPX1 MluI Age1 with PrA1/1r and PrA2/2r Mlu1 and Age1)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1066" data-index="408" style="margin-left:0;">miniprep pDOP 1-20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1067" data-index="409" style="margin-left:0;">Test Cut pDOP1-20 with NheI-HF and StuI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1068-item" data-formattable="true" data-index="410" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-29-2016 pDOP 1-10.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="oTpT0Hc2exGh4zcE7xKQDZiOmLlyvz4ojyI5rZOR-06-29-2016_pDOP_1-10.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1068" data-index="410">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1070-item" data-formattable="true" data-index="411" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-29-2016 pDOP 11-20.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="3z9wz2wOTF7PeCQGtK5K0iDgNZloPD3lrxNaMwoL-06-29-2016_pDOP_11-20.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1070" data-index="411">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1072" data-index="412" style="margin-left:0;">Transfected pGOP 3,5,6,8,10,14,15,19 of iRFP, BFP, GFP, and mRuby </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1073-item" data-formattable="true" data-index="413" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.16.09.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ffaCORLZdCeyl1DW9PulPv97fd4H4uHkRTf5pwan-Screen_Shot_2016-08-01_at_12.16.09.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1073" data-index="413">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1075" data-index="414" style="margin-left:0;">PIcked pGPX2 and pGPX3 colonies </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1076" data-index="415" style="margin-left:0;">Reannealed-phosphorylated- ligated - and transformed pDOPs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1077" data-index="416" style="margin-left:0;">recut pDOPs</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1078-item" data-formattable="true" data-index="417" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-30-2016 recut pDOPs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Qj9QP4QxBwOd5MMx56qn71vzQpgPDWIn2rL1aUpz-06-30-2016_recut_pDOPs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1078" data-index="417">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1080" data-index="418" style="margin-left:0;">Miniprep of GPX 2-1/2/3 and GPX 3-1/2/3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1081" data-index="419" style="margin-left:0;">Test-cut GPX 2 and 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1082" data-index="420" style="margin-left:0;">Picked colonies and grew up pDOP 1-4; 6-11; 14-20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1083" data-index="421" style="margin-left:0;">Ran FACS (flow cytometry)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1084" data-index="422" style="margin-left:35px;">Did not test BFP due to wrong transfection marker</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1085" data-index="423" style="margin-left:0;">PCR clean up of 2485; 2487; and 2489 with NotI and Cip</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1086" data-index="424" style="margin-left:0;">Gel extraction of 2484, 2486, 2488, and 2534 digested with AscI and NotI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1087-item" data-formattable="true" data-index="425" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-01-2016 pGPX2 3.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vNghMX61p19d8X0d3jORtlxpChGR5S7WO8hB2CDB-07-01-2016_pGPX2_3.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1087" data-index="425">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1089" data-index="426" style="margin-left:0;">Miniprep and test cut pDOP 1-4; 6-11; 14-20 colonies 1 and 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1090" data-index="427" style="margin-left:35px;">digested with NheI and PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1091" data-index="428" style="margin-left:0;">Vectors pGPX2 and pGPX3 are sequenced verifeid. pGOP 66,34, and 26 could not be sequenced properly (quintara error)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1092" data-index="429" style="margin-left:0;">Reenoculate pGEX and GFP pGOP 3, 5, 6, 8, 10, 14, 15, 19 gh54</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1093" data-index="430" style="margin-left:0;">Miniprep pGEX and GFP pGOP 3, 5, 6, 8, 10, 14, 15, 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1094" data-index="431" style="margin-left:0;">Gibson and transform pREC 1, 2, 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/4</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1095" data-index="432" style="margin-left:0;">Passage HEK cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1096" data-index="433" style="margin-left:0;">Inoculate pREC 1, 2, 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1097" data-index="434" style="margin-left:0;">Miniprep pREC 1, 2, 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1098" data-index="435" style="margin-left:0;">Enoculate BW1720</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1099" data-index="436" style="margin-left:0;">Digested BW1720, and BW1942-43 with BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1100" data-index="437" style="margin-left:0;">Digested BW758, and BW762-65 with AgeI and EcoRI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1101" data-index="438" style="margin-left:0;">Digested DC 100 with NheI and Cip</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1102" data-index="439" style="margin-left:0;">Digested pGPX1-4 with Xbha and Bbsi</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1103-item" data-formattable="true" data-index="440" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-05-2016 dc100, pGPX1:2:3:4.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="AYg1ftPlRSrVCzZohTRniAOeHZ7E3LRMATOVOJpj-07-05-2016_dc100_pGPX1234.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1103" data-index="440">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1105-item" data-formattable="true" data-index="441" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-05-2016 BW contstructs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="EcsdVNTZozlZ5Uel33oVRR4mfEAiumKlmI9LIyHu-07-05-2016_BW_contstructs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1105" data-index="441">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1107-item" data-formattable="true" data-index="442" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-05-2016 pREC.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="HeoC8UONPeRA646xLXzYEBjt4yxV6BsneIjefjJZ-07-05-2016_pREC.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1107" data-index="442">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1109" data-index="443" style="margin-left:0;">Transfected HEK cells for gRNA orthogonality experiment</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1110-item" data-formattable="true" data-index="444" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.15.02.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="smQSiF4pGbCfYb9gknZvgPQFIxnMkFlUmpMgzzpL-Screen_Shot_2016-08-01_at_12.15.02.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1110" data-index="444">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1112" data-index="445" style="margin-left:0;">Miniprep pGEX, GFP pGOP, and mRuby pGOP 6& 14</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1113" data-index="446" style="margin-left:0;">Digested 1720 with AgeI and EcoRI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1114-item" data-formattable="true" data-index="447" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-06-2016 1720.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="k18rRvOubLEXGhDvdawlLwvlnDtq0piBXcFT9k87-07-06-2016_1720.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1114" data-index="447">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1116" data-index="448" style="margin-left:0;">Redigested 2485, 2487, and 2489 with NotI and Cip</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1117-item" data-formattable="true" data-index="449" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-06-2016 2485 2487 2489.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ahXE7E33j1cHLOnrIJywpGtvWqxBv9k8vIW0MiCN-07-06-2016_2485_2487_2489.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1117" data-index="449">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1119" data-index="450" style="margin-left:0;">Ligated and transformed pBEX1-24 pPV1,5-8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1120" data-index="451" style="margin-left:0;">Retransformed pGEX6 and 14 pGOP26,34,66,74</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1121" data-index="452" style="margin-left:0;">Made LB + Carb plates</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1122" data-index="453" style="margin-left:0;">Picked colonies for pBEX 1 - 24, pGEX 6 and 14, GFP and mRuby pGOPs 6 and 14, adn pPVs 1, 5, 6, 7, 8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1123" data-index="454" style="margin-left:0;">Miniprep pGEX 1-4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1124" data-index="455" style="margin-left:0;">Ran FACs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1125" data-index="456" style="margin-left:0;">Grew more 2534</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1126-item" data-formattable="true" data-index="457" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_9323 copy.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="hJJ5fIChPvpyFYLYqEm0vuPgD6EUUy7Wmyuo58oC-IMG_9323_copy.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1126" data-index="457">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1128" data-index="458" style="margin-left:0;">Grew up pPV7 and pBEX 10, 12, 13, 17, 18, 22</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1129" data-index="459" style="margin-left:0;">Growing more 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1130" data-index="460" style="margin-left:0;">Gibsoned pREC1, 2, and 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1131" data-index="461" style="margin-left:0;">Passaged HEK cells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1132" data-index="462" style="margin-left:0;">Cell Stock pPV7 and pBEX 10, 12, 13, 17, 18, and 22</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1133" data-index="463" style="margin-left:0;">Digested pGPX2, mRuby2, and pGOP 1, 2, 4, 6, 7, 9, 11, 12 13, 16, 17, 18, and 20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1134" data-index="464" style="margin-left:35px;">mRuby2 and pGOPs digested with MluI and AgeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1135" data-index="465" style="margin-left:35px;">pGPX2 digested with BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1136" data-index="466" style="margin-left:35px;">Ran on a gel and gel extracted </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1137-item" data-formattable="true" data-index="467" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-12-2016 pGOP mRuby2 pGPX2.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tpR1ppA9J1tlaKS7r1vZDs6ip64C48QDXpDIYtUZ-07-12-2016_pGOP_mRuby2_pGPX2.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1137" data-index="467">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1139" data-index="468" style="margin-left:0;">Miniprep pGPX2, 1720, 1942-44</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1140" data-index="469" style="margin-left:0;">Digested pGPX2, 1720, 1942-44 with ________________</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1141" data-index="470" style="margin-left:0;">Re-digested pGPX2 (bbs1, extract 3kb band) ,mRuby2(mlu1, age1; extract 700bp band) ,and pGOP21 (mlu1,age1, extract 3kb band)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1142" data-index="471" style="margin-left:35px;">ran on gel and gel extracted</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1143-item" data-formattable="true" data-index="472" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-13-2016 pGPX2,mRuby,pGOP21.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="c1UXJTRKyVdyjr4VyNuHyV2FMAX4FpfHit9D3Thr-07-13-2016_pGPX2mRubypGOP21.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1143" data-index="472">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1145" data-index="473" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1146" data-index="474" style="margin-left:0;">Ligating and transforming </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1147" data-index="475" style="margin-left:35px;">pGOP 21 22 24 27 29 31 32 33 34 36 37 38 40 41 42 44 47 49 51 52 53 56 57 58 60 61 62 64 66 67 69 71 72 73 76 77 78 80</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1148" data-index="476" style="margin-left:0;">Digesting </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1149" data-index="477" style="margin-left:35px;">1270 758 763 765 with AgeI and EcoRI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1150-item" data-formattable="true" data-index="478" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 758 763 765 1720.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="AgRuyIo3HAhv87gPqnu0SOtg9rsP0plHncSYYIEA-07-14-2016_758_763_765_1720.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1150" data-index="478">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1152" data-index="479" style="margin-left:0;">Circuit Designs Digesting</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1153" data-index="480" style="margin-left:35px;">Part1 AscI NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1154" data-index="481" style="margin-left:35px;">Part2 <span style="color:#344043"><span style="background-color:#FFFFFF">AscI NotI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1155" data-index="482" style="margin-left:35px;">Part3 <span style="color:#344043"><span style="background-color:#FFFFFF">AscI NotI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1156" data-index="483" style="margin-left:35px;">Part4 <span style="color:#344043"><span style="background-color:#FFFFFF">AscI NheI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1157" data-index="484" style="margin-left:35px;">Dest <span style="color:#344043"><span style="background-color:#FFFFFF">EcoRI NotI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1158" data-index="485" style="margin-left:0;">Circuits (P1-P2-P3-P4)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1159" data-index="486" style="margin-left:0;">pBEX1-pBEX6-pBEX15-pBEX24</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1160" data-index="487" style="margin-left:0;">pBEX1-pBEX10-pBEX19-pBEX20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1161" data-index="488" style="margin-left:0;">pBEX5-pBEx18-pBEX19-pBEX16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1162" data-index="489" style="margin-left:0;">pBEX5-pBEX2-pBEX15-pBEX8</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1163-item" data-formattable="true" data-index="490" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 pBEX 8 16 20 24.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tMPp5VtpeLwTg6YY1m32xCeBDdynzrqJrstcO2Ie-07-14-2016_pBEX_8_16_20_24.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1163" data-index="490">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1165-item" data-formattable="true" data-index="491" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 pBEXs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="CEfg9psNCtUnY7b3DJ1quiDzL8vPYyZNMKnfSw8y-07-14-2016_pBEXs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1165" data-index="491">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1167" data-index="492" style="margin-left:0;">Test cut 2578, 2579, 2580 (formely pRECs)</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1168-item" data-formattable="true" data-index="493" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 2578 2589 2580.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="PpX97l1ddkWyX0XopiDPOc0NSzwuG8jZ4TYIsyF6-07-14-2016_2578_2589_2580.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1168" data-index="493">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1170" data-index="494" style="margin-left:0;">Miniprep 2578, 2579, and 2580 3-7 & testcut</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1171-item" data-formattable="true" data-index="495" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-15-2016 2578 2579 2580 test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="gJObOL4E1ioK87khT7qzU3Ot37dfKuyAASv4ucbN-07-15-2016_2578_2579_2580_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1171" data-index="495">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1173" data-index="496" style="margin-left:0;">2578-2 and 2579-1 were sequenced verified adn moved to cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1174" data-index="497" style="margin-left:0;">Minipreped 1716 pDest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1175" data-index="498" style="margin-left:0;">Digested pDest with EcoRI and NotI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1176-item" data-formattable="true" data-index="499" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-15-2016 1716.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="MOIIA8OvBcxCPCCk9JccqWgrcHtMoYaIWN55yIOp-07-15-2016_1716.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1176" data-index="499">

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="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1178" data-index="500" style="margin-left:0;">Religated pPV1, pPV6, and pPV8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1179" data-index="501" style="margin-left:0;">Picked new pGOP colonies for GFP only</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1180" data-index="502" style="margin-left:0;">Miniprepped new GFP clonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1181" data-index="503" style="margin-left:0;">Minprepped 2580-8 through 2580-17</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1182" data-index="504" style="margin-left:0;">Transformed pCir1 pCir2 and pCir3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1183" data-index="505" style="margin-left:0;">Test Cuts for 2580 aditional colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1184" data-index="506" style="margin-left:0;">Test Cuts for pPV1, pPV6, and pPV8 additional colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1185" data-index="507" style="margin-left:0;">Test Cuts for pGOP GFP additional colonies</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1186" data-index="508" style="margin-left:0;">Test Cuts for pCIR1, pCir2, and pCir3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1187" data-index="509" style="margin-left:0;">Test Cuts for pGOp1 and 2 under BFP and mRuby</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1188" data-index="510" style="margin-left:0;">Split HEK cells 1:10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1189" data-index="511" style="margin-left:0;">Created two 48 well plates at 200,000 cells/ml for transfection of BFP pGOP constructs </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1190" data-index="512" style="margin-left:0;">Created two T175plates with 25ml of 100,000cells/ml for stable integration</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1191" data-index="513" style="margin-left:0;">Midiprep BW361, BW465, BW471, BW 474, 2578, and 2579</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1192" data-index="514" style="margin-left:0;">Digested pBEX 19 with AscI and NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1193" data-index="515" style="margin-left:35px;">Gel extracedracted pBEX 19</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1194-item" data-formattable="true" data-index="516" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07192016 pBEX19.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="VF3g6RErnr18sRETBB2fk628MQB5A60yiIKhBmKc-07192016_pBEX19.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1194" data-index="516">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1196" data-index="517" style="margin-left:0;">Digested pCir 1-3, 1-1, 3-2, 3-3 with Not1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1197-item" data-formattable="true" data-index="518" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07192016 pcirtestcuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="2mYxgnPtjdl4LeKcIU5IXraiK65M0KUurg5ocznc-07192016_pcirtestcuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1197" data-index="518">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1199" data-index="519" style="margin-left:0;">Transfected BFP</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1200-item" data-formattable="true" data-index="520" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 00.01.59.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="s8BAZnYNjLQiiFMHdY3XY7Ysi9s6QN5WhsYk430T-Screen_Shot_2016-08-01_at_00.01.59.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1200" data-index="520">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1202-item" data-formattable="true" data-index="521" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_0128.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ci78q40lvuDsyThTfcxUy1UcOaIJ5R3rUk5qUnRA-IMG_0128.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1202" data-index="521">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1204" data-index="522" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1205" data-index="523" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1206" data-index="524" style="margin-left:0;">Performed FACs on the BFP transfected cells. Tried to create the data in MEFL but had trouble with getting the channel names to work with the software.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1207" data-index="525" style="margin-left:0;">Split HEK cells to P16. Expanded them into large T175 plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1208" data-index="526" style="margin-left:0;">Transfected the HEK cells in T175 with 2578 and 2579. Brought media volume up to 50ml to do so</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1209-item" data-formattable="true" data-index="527" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_1338.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="pXXqtxKOLiZO7PkSPbXESQXZpxRCuXZBklYdPAha-IMG_1338.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1209" data-index="527">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1211-item" data-formattable="true" data-index="528" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07222016 pCir test cut2.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="uysFA617h6fYOu2dAlEOhJMw3GFR6PEOZmY3Rxqz-07222016_pCir_test_cut2.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1211" data-index="528">will did a testcut of pCir1 and pCir3. sent one of each in for sequencing.

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1213" data-index="529" style="margin-left:0;">added puromyocin to the two stable line integrations of 2578 and 2579. 2ug per ml so we added 10ul of puromyocin to 50ml of media in the flask</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1214" data-index="530" style="margin-left:35px;">many more cells ttoday than yesterday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1215" data-index="531" style="margin-left:0;">Sent in a sample of the pGOP41 used in the previous transfection to troubleshoot what went wrong and why that particular gRNA was not working with BFP</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1216-item" data-formattable="true" data-index="532" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07232016 BFP PCR.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="mw7RHlZVpdgg12fGyVVs6E554HOZgFRSvR5uhqFg-07232016_BFP_PCR.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1216" data-index="532">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/24</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1218" data-index="533" style="margin-left:0;">Split the stable line integration HEK cells of 2578 and 2579 each into two new T175 flasks because they were too crowded. Added puromyocin to the fresh media.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1219" data-index="534" style="margin-left:0;">Transformed (GFP) pGOP 26, 27, 32, 33, 38, 40</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1220" data-index="535" style="margin-left:35px;">(BFP): pGOP 41, 42, 43, 44, 46, 47, 49, 51, 52, 53, 56, 57, 58, 60</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1221" data-index="536" style="margin-left:35px;">(mRuby): pGOP 61, 62, 64, ,66, 67, 69, 71, 72 73, 76, 77, 78, 80</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1222" data-index="537" style="margin-left:35px;">PIcked colonies from all transformed GFP pGOPs and less successful BFPs and mRubys</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1223" data-index="538" style="margin-left:35px;">Prepped overnight ligation for the BFPs and mRubys</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1224" data-index="539" style="margin-left:70px;">due to miscommunication, the ligation mix was made incorrectly but we went ahead anyway</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1225" data-index="540" style="margin-left:35px;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1226-item" data-formattable="true" data-index="541" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07252016 pGOP cuts and BFP mRUBY.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="RtdkVleUNsEWpu7rS0sCdUJudmeYNPr5ccpZfleL-07252016_pGOP_cuts_and_BFP_mRUBY.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1226" data-index="541">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/26</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1228" data-index="542" style="margin-left:0;">Digested pBEX 1,2,5,6,16,19 (AscI and NotI except 16 which was AscI and NheI) and 1716 (EcoRI and NotI) and pGOP68 and 35 (MluI and AgeI)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1229" data-index="543" style="margin-left:35px;">did not extract the pGOPs or 1716</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1230" data-index="544" style="margin-left:35px;">extracted the rest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1231" data-index="545" style="margin-left:35px;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1232-item" data-formattable="true" data-index="546" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07262016 pBEX cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="L7yNw3j3sjnY8spe2uL5pU6ANt3KhSZVW3BuiR0w-07262016_pBEX_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1232" data-index="546">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1234" data-index="547" style="margin-left:0;">Inoculated pBEX 1,6,16,19, and 1716 and pGPX2 (so we could drop in the new oligos)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1235" data-index="548" style="margin-left:0;">Transformed DNA from overnight ligation </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1236" data-index="549" style="margin-left:0;">made Cam plates</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1237-item" data-formattable="true" data-index="550" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07262016 pgop.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="nEJFDNTscb6ROG7ROFWIiHy1IxLZob5fm08egeqN-07262016_pgop.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1237" data-index="550">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1239" data-index="551" style="margin-left:0;">Transformation did not work</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1240" data-index="552" style="margin-left:0;">Digested 1716 (EcoRI and NotI) and pGOP68 and pGOP35 (MluI and AgeI)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1241" data-index="553" style="margin-left:35px;">gel extracted</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1242-item" data-formattable="true" data-index="554" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07272016 pGOP35, 68 1716.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="lkneClrCTjyfxrNSSVGfxRWGNp9ZWEpzzHyW95yV-07272016_pGOP35_68_1716.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1242" data-index="554">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1244" data-index="555" style="margin-left:0;">annealed multimerized oligos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1245" data-index="556" style="margin-left:0;">digested more pGPX</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1246-item" data-formattable="true" data-index="557" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07272016 pGOP and pGPX digest.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="nH90ToeepVdGj9sKTM32svri3O2ahzppjXWNnN7f-07272016_pGOP_and_pGPX_digest.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1246" data-index="557">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1248" data-index="558" style="margin-left:0;">Diluted all the DNA for the transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1249" data-index="559" style="margin-left:0;">Transfected HEK cells with the "mini circuit" (1902/1903, pGOP1, pGOP22, BW390 (constituitive cre) and 2196 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1250" data-index="560" style="margin-left:35px;">also transfected pGOP22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 to test all of the GFPs. we did not have pGOP 21 and we did not have pGOP 40. We did not have pGEX11 to transfect so pGOP31 should not work. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1251" data-index="561" style="margin-left:0;">organized our DNA boxes</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1252-item" data-formattable="true" data-index="562" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.13.53.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tdruuEqVouvF8zQROLwEpvxUFVtLxBIPC2BA5722-Screen_Shot_2016-08-01_at_12.13.53.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1252" data-index="562">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1254" data-index="563" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1255" data-index="564" style="margin-left:0;">Digested more BFP and mRUBY</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1256" data-index="565" style="margin-left:35px;">26ul pGOP55 30ulpGOP64</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1257" data-index="566" style="margin-left:35px;">1.5 of MLU1-HF &Age1-HF 1.5 of Mlu1-HF &Age1-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1258" data-index="567" style="margin-left:35px;">5 cutsmart 5 cutsmart</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1259" data-index="568" style="margin-left:35px;">20 h2o 16 h2o</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1260-item" data-formattable="true" data-index="569" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07292016 mRuby BFP.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="15vxSSYYlKurHynUbhYvPjfImfTmgSlOxEI2O83k-07292016_mRuby_BFP.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1260" data-index="569">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1262" data-index="570" style="margin-left:0;">ligated to create pGOP41, 51, 82, 83 to se what wasnt working with our cloning by testing our T4 ligase vs Wong labs t4 ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1263" data-index="571" style="margin-left:0;">Testcut pCir1-6, -7, -8, -9, -10, -11, -12, -13, -14, -15, pCir3-4 with Acs1 Not1-HF</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1264-item" data-formattable="true" data-index="572" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07292016 pCir test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="wBPNZHZKB0avYwAF0xnlfFOOnw7f9Lo2LKeR7i7I-07292016_pCir_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1264" data-index="572">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1266" data-index="573" style="margin-left:0;">Cell stocked pGOP38-4, pGOP42-1, pGOp43-1, pGOP44-1, pGOP26-5, pGOP27-4, pGOP32-4, pGOP32-4, pGOP33-4, pGOP60-1, pGOP64-1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1267" data-index="574" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1268" data-index="575" style="margin-left:0;">performed FACs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1269" data-index="576" style="margin-left:0;">Split Deboki's cells for her and took a t175 plate at 1:10. (passage again on monday)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1270" data-index="577" style="margin-left:0;">Moved the stable line integrations to T50 plates. added 2ul of puromycin to 10ml of media</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1271" data-index="578" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1272" data-index="579" style="margin-left:0;">Picked colonies for GOP 82, and 41, 51 (no colonies for 83), pCIR1s that Ben made (picked 10 colonies)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1273" data-index="580" style="margin-left:0;">Met with Leidy to discuss what we are doing wrong with our ligation and transformation protocol. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1274" data-index="581" style="margin-left:0;">Re did digesestion of BFP and mRuby from pGOP 43 and 64, respectively. (age1 mlu1)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1275" data-index="582" style="margin-left:0;">Redigested pgpx2 to drop in the oligos.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1276" data-index="583" style="margin-left:0;">Religating fluorescence into regular pGOPs</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1277" data-index="584" style="margin-left:0;">Picked colonies of pGOP 66-1*(failed),-2, 67-1*(failed),-2, 71-1*(failed),-2, 73-1*(failed),-2, 76-1*(worked),-2, 46-1*(worked),-2, 47-1*(failed),-2., 51-1*(failed),-2, 52-1*(failed), 53-1*(worked),-2, 56-1(failed)*,-2, 49-1*(failed),-2, 69-1*(failed sequencing reaction),-2, 41-1*(worked),-2,-3 -4,-5, 51-1,-2,-3,-4,-5,-6, 40-1,-5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1278" data-index="585" style="margin-left:0;">pGOP 81-1,-2, 82-1*,-2, 83-1,-2,84-1,-2, 85-1,-2, 82-0* (-0 means grew up day before), 86-1,-2,87-1,-2,88-1*,-2, 89-1,-2, 90-1,-2, 83-0</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1279" data-index="586" style="margin-left:0;">pCIR1 -16,-17-,-18, -19,-20*,-21,-22,-23, -24,-25*,-26*,-27*, Ben's1, Ben's2*</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1280" data-index="587" style="margin-left:35px;">(starred**** ones sent for sequencing)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/31</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1281" data-index="588" style="margin-left:0;">miniprep of all of the colonies picked yesterday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1282" data-index="589" style="margin-left:0;">Ran test cuts of all of the colonie picked</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1283-item" data-formattable="true" data-index="590" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pCIR test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="lmLQvS2CgGQec3efgEz7AkI6BRwb5iolJf7vo9Nk-07312016_pCIR_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1283" data-index="590">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1285-item" data-formattable="true" data-index="591" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pGOP80 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="EXHnp5yYGcu8OaP4vaG5e17c02rHxWuWIbKdeTWF-07312016_pGOP80_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1285" data-index="591">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1287-item" data-formattable="true" data-index="592" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pGOP test cuts more.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="86Dw4nWHylAkYMygxEDNMnaYLHdopKA8K52TLPmd-07312016_pGOP_test_cuts_more.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1287" data-index="592">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1289-item" data-formattable="true" data-index="593" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pGOP cuts again.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="J4n8TC5hpL1JLyx91BIIZCf7Zsr3WLV9XXQc8D0D-07312016_pGOP_cuts_again.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1289" data-index="593">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1291" data-index="594" style="margin-left:0;">split HEK cells 1:10 from large flask into small flask.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1292" data-index="595" style="margin-left:0;">let stable line of cells grow for another day.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1293" data-index="596" style="margin-left:0;">Grew up.... pGEX11, pGEX12, pGEX13, pGEX17, pGEX18, pGEX20, pGOP6(x2),pGOP21, pGOP22, pGOP23, pGOP24, pGOP25, pGOP26, pGOP27, pGOP28, pGOP29, pGOP30, pGOP31, pGOP32, pGOP33, pGOP34, pGOP35, pGOP36, pGOP37, pGOP38, pGOP39, pGOP42, pGOP43, pGOP44(x2), pGOP45(x2), pGOP50, pGOP54, pGOP55(x2), pGOP59, pGOP68, pGPX2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1294" data-index="597" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1295" data-index="598" style="margin-left:0;">(Will and Marisa) miniprepped pGEX11, pGEX12, pGEX13, pGEX17, pGEX18, pGEX20, pGOP6(x2),pGOP21, pGOP22, pGOP23, pGOP24, pGOP25, pGOP26, pGOP27, pGOP28, pGOP29, pGOP30, pGOP31, pGOP32, pGOP33, pGOP34, pGOP35, pGOP36, pGOP37, pGOP38, pGOP39, pGOP42, pGOP43, pGOP44(x2), pGOP45(x2), pGOP50, pGOP54, pGOP55(x2), pGOP59, pGOP68, pGPX2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1296" data-index="599" style="margin-left:0;">Grew up pGOP41, pGOP46, pGOP53, pGOP71, pGOP76 for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1297" data-index="600" style="margin-left:0;">Split the stable line HEK cells into new T75 flasks (p18)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1298" data-index="601" style="margin-left:0;">suspended the crRNA gBlocks oligos in 1xTE</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1299" data-index="602" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1300" data-index="603" style="margin-left:0;">Digested pGPX2 with BBs1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1301-item" data-formattable="true" data-index="604" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160803 jeffery's stuff.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="aH19yZLA39RIzgKGv8HYVq9SKipBdFIP3NvTDouu-20160803_jefferys_stuff.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1301" data-index="604">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1303" data-index="605" style="margin-left:0;">DIgested pGOP76-1 and pGOP 41-1 for mRuby and BFP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1304" data-index="606" style="margin-left:0;">Transformed pGOPs 57, 58, 61, 62, 66, 77, 78, 81, 84, 85, 86, 87, 88, 89 using Leidys transformation protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1305" data-index="607" style="margin-left:35px;">use Ben's Top Ten cells, let cells sit on ice</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1306" data-index="608" style="margin-left:35px;">add 10ul of 5x KCM to ligation mix</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1307" data-index="609" style="margin-left:35px;">add 30ul of DI H2O to ligation mix</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1308" data-index="610" style="margin-left:35px;">add all 50 ul to cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1309" data-index="611" style="margin-left:35px;">plated 95ul of cells onto 1/2 plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1310" data-index="612" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1311" data-index="613" style="margin-left:0;">Sent sequencing pGOP 47-1(worked), 49-1(worked), 51-2(worked), 52-1(worked), 56-2 (failed), 67-1(worked), 69-1(worked), 71-1(FAIL), 72-1(worked), 73-1(resendfor seuquencing), 83-0(resend for sequencnig)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1312" data-index="614" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1313" data-index="615" style="margin-left:0;">Cell stocked pGOP41, pGOP46, pGOP53(need to throw awayb/c miscommunication), pGOP76 (throw away b/c miscommunication)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/4</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1314" data-index="616" style="margin-left:0;">(Marisa) Picked 2 colonies for pGOPs 57, 58, 61, 62, 66, 77, 78, 81, 84, 85, 86, 87, 88, 89</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1315" data-index="617" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1316" data-index="618" style="margin-left:0;">(Kami) Split wildtype cells 1:20 to P19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1317" data-index="619" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1318" data-index="620" style="margin-left:0;">(Jeffery) dimerized guides 1, 3, 8, 13 in various configurations with each other (1-3, 3-1, 8-13, 13-8)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1319" data-index="621" style="margin-left:35px;">this involved PCR and a gel extraction</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1320" data-index="622" style="margin-left:35px;">Designed decoders </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1321-item" data-formattable="true" data-index="623" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 dec2.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="yPemrWs1yagTZ6vMpTl7yGUPMNKryQNboZ5U7ame-20160805_dec2.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1321" data-index="623">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1323-item" data-formattable="true" data-index="624" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 decoder parts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="uvUQsVbJ5DrTUjgsOEnbT8zwd90VrNYENZ8nmn6y-20160805_decoder_parts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1323" data-index="624">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1325" data-index="625" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1326" data-index="626" style="margin-left:0;">(Will) Grew up pGOP 47, 49, 51, and 82-0 for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1327" data-index="627" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1328" data-index="628" style="margin-left:0;">Due to a miscommunication error, we had to retransform and plate pGOP52-1, pGOP53-1, pGOP67-1, pGOP69-1, pGOP71-2, pGOP72-1, pGOP73-1. We did this with our typical transformation protocol.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1329" data-index="629" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1330" data-index="630" style="margin-left:0;">(Marisa and Will) Miniprepeed pGOPs 57, 58, 61, 62, 66, 77, 78, 81, 85, 86, 87, 88, and 89</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1331" data-index="631" style="margin-left:35px;">Due to a miscommunication error, miniprepped pGOP 47, 49, 51, and 82-0</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1332" data-index="632" style="margin-left:0;">(Will) Gibsoned </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1333" data-index="633" style="margin-left:35px;">pCir 2 using 1716, A, B, C, D</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1334" data-index="634" style="margin-left:35px;">pCir 2 using 1716 Part1/2, Part 3/4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1335" data-index="635" style="margin-left:35px;">pCir 2 usng 1716, Decoder 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1336" data-index="636" style="margin-left:35px;">pCir 4 using 1716, lambda, Part 3/4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1337" data-index="637" style="margin-left:35px;">pCir 1 using 1716, alpha, Bex19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1338" data-index="638" style="margin-left:35px;">pCir 1 using 1716, Part 1/2, Bex19, Bex16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1339" data-index="639" style="margin-left:35px;">pCir 1 using 11716 Bex 1, Bex6, Bex19, Bex16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1340" data-index="640" style="margin-left:35px;">pCir 3 using 1716, Bex5, Bex2, Bex19, Bex16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1341" data-index="641" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1342" data-index="642" style="margin-left:0;">(Kami) Split stabel line (2578 and 2579) 1:10 in T75</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1343" data-index="643" style="margin-left:0;">(Rachel) Test-cut pGOPs <span style="color:#344043"><span style="background-color:#FFFFFF">57, 58, 61, 62, 66, 77, 78, 81, 85, 86, 87, 88, and 89</span></span></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1344-item" data-formattable="true" data-index="644" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 pGOP57-78 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="PJqzNS2X4CDpBCI6RczRnLaP1Ryj7F2fHwtowQLT-20160805_pGOP57-78_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1344" data-index="644">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1346-item" data-formattable="true" data-index="645" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 pGOP81-89 test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ScjbHlUMbmpZuezUUx2AhTxJCsBXMkEYJjW9XIVc-20160805_pGOP81-89_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1346" data-index="645">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1348" data-index="646" style="margin-left:0;">(Rachel) digested pGPX2 (5mg) with 3ul of BbsI. Double digested - too low of concentraiton to proceed with ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1349" data-index="647" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 8/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1350" data-index="648" style="margin-left:0;">(Will) grew up </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1351" data-index="649" style="margin-left:0;">(Jeffery) <span style="color:#000000">Digested pgpx2 with bbsi</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1352" data-index="650" style="margin-left:0;">(kami) made WT Hek plates with Yash and split WT and 2578 and 2579 integrated cells 1:10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1353" data-index="651" style="margin-left:0;">(will) cell stocked pGPX2 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1354" data-index="652" style="margin-left:0;">(Will) Test cut the pCir gibsons with Asc1 and Not1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1355" data-index="653" style="margin-left:0;">(Will) transformed pGOP 84. 86, 87, 88, 89, 90 with a ligation using Leidy's protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1356" data-index="654" style="margin-left:35px;">transformed pgOP47, 49, 51, 82-0 without a ligation using Leidy's protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1357" data-index="655" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/9</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1358" data-index="656" style="margin-left:0;">(Rachel) Growing pCir4_2_4 from stab plate as sequencing indicated it worked!</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1359" data-index="657" style="margin-left:0;">(Rachel) Picked colonies from pGOP 81,84,86,87,88,89,90</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1360" data-index="658" style="margin-left:0;">(Rachel) made more LB + Cam broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1361" data-index="659" style="margin-left:0;">(Kami) autoclaved tips and agar</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1362" data-index="660" style="margin-left:0;">(Marisa and Kami) made carb plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1363" data-index="661" style="margin-left:0;">(Jeffrey) performed overhang extension PCRs on G-blocks (that make decoder) to build Circuit 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1364" data-index="662" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/10</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1365" data-index="663" style="margin-left:0;">(Marisa and Will) miniprep pGOP 81,84,86,87,88,89,90</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1366" data-index="664" style="margin-left:35px;">test cut with speI and BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1367" data-index="665" style="margin-left:0;">(Marisa) Cell Stock pGOP 47, 49, 51, and 82; pCir 4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1368" data-index="666" style="margin-left:35px;">miniprep pCir 4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1369" data-index="667" style="margin-left:0;">(Jeffrey) Digested Bex 19 and 16</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1370" data-index="668" style="margin-left:0;">(Kami and Marisa) grew up BW 363, 390, 391; pSB1C3; and T40 for midiprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1371" data-index="669" style="margin-left:0;">(Kami) split WT, 2578, 2579 HEK cells into T175</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1372" data-index="670" style="margin-left:0;">(Jeffrey) Picked Colonies for 87 and 88</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1373" data-index="671" style="margin-left:0;">(Marisa) Midiprep BW 363, 390, 391, T40, pSB1C3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1374" data-index="672" style="margin-left:0;">(Jeffrey) MIinprepped and test 87 and 88... picked colonies for 90. Transformed interlab study plasmids</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1375" data-index="673" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Marisa) Midiprep BW 363, 390, 391, T40, pSB1C3</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1376" data-index="674" style="margin-left:35px;">Cell stock pGOP 87</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1377" data-index="675" style="margin-left:0;">(Marisa and Rachel) Gel extracted </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1378" data-index="676" style="margin-left:0;">(Jeffrey) Mini prep and test cut 90... Retransformed interlab device 1</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 8/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1379" data-index="677" style="margin-left:0;">(Marisa and Rachel) Cell stock Interlab study: Pos 1, Neg 1, Part 2-1, Part 2-2, Pos 2, Neg 2, Part 3-1, Part 3-2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1380" data-index="678" style="margin-left:35px;">Miniprep Interlab Study and pGOP 56-3/4/5, pGOP 83-5/6/7/8/9</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1381" data-index="679" style="margin-left:0;">(Rachel) Digested pGOPs 56 and 83 with SpeI and XmaI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1382" data-index="680" style="margin-left:0;">(Marisa) Test Cut pGOPs 56 and 83</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1383" data-index="681" style="margin-left:0;">(Rachel) Ligate </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1384" data-index="682" style="margin-left:0;">(Jeffrey and Marisa) picked and grew up colonies: pGOP 57-1, 61-1/2/3, 62-1/2/3, 66-1/2/3, 77-1/2/3, 78-1/2/3, 80-1/2/3, 88-1/2/3/4/5, 90-1/2/3/4/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1385" data-index="683" style="margin-left:0;">(Kami and Marisa) Diluted and nanodropped </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1386" data-index="684" style="margin-left:0;">(Kami) made 6 plates for transfection. 3 with WT, 2 with stable line integrations of 2578 and 2579 each.</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1387" data-index="685" style="margin-left:0;">(Marisa) miniprepped <span style="color:#344043"><span style="background-color:#FFFFFF">pGOP 57-1, 61-1/2/3, 62-1/2/3, 66-1/2/3, 77-1, 78-1/2/3, 80-1/2/3, 88-1/2/3/4/5, 90-1/2/3/4/5</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1388" data-index="686" style="margin-left:0;">(Will) Digested Marisa's minipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1389" data-index="687" style="margin-left:0;">(Kami and Rachel) 7:30 pm transfected HEK cells to test the multimerization and the circuit</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1390" data-index="688" style="margin-left:35px;">for the circuit. Transfected 1/8 of 250ng of each component. (2 pgops made up 1/8 of the ng transfection and the extra was filled with Luis's pSB2C3 blank)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1391" data-index="689" style="margin-left:0;">(Kami And rachel) 2 hours after transfection, we added 4OHT/Absistic acid/Rapalog to appropriate wells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1392" data-index="690" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1393" data-index="691" style="margin-left:0;">(Kami and Rachel) prepared the transfected cells for FACS, and ran facs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1394" data-index="692" style="margin-left:0;">(Kami) Cells stocked stabel lines of HEK 2578(m150) and 2579 (m151)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1395" data-index="693" style="margin-left:35px;">trypsonize cel</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1396" data-index="694" style="margin-left:35px;">neutralize with media</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1397" data-index="695" style="margin-left:35px;">spin cells down</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1398" data-index="696" style="margin-left:35px;">remove media and break up pellet. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1399" data-index="697" style="margin-left:35px;">resuspend pellet in 90%FBS 10%DMSO (1ml per cryotube)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1400" data-index="698" style="margin-left:35px;">one T75 makess 2-3 cell stocks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1401" data-index="699" style="margin-left:0;">(Marisa) Made LB Broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1402" data-index="700" style="margin-left:35px;">inoculated T40 and BW363 in falcon tubes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1403" data-index="701" style="margin-left:35px;">turn LB Broth into LB + Carb broth</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1404" data-index="702" style="margin-left:0;">(Kami) made cam agar and broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1405" data-index="703" style="margin-left:35px;">miniprep pGOP 2, 6, 83-11/12/13/14/15</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1406" data-index="704" style="margin-left:0;">(Marisa) made Cam plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1407" data-index="705" style="margin-left:35px;">Cell stocked pGOP 57, 61, 80, 88, and 90</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1408" data-index="706" style="margin-left:35px;">inoculated 1mL of each T40 and BW363 sampled into flasks with 150 mL LB + Carb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1409" data-index="707" style="margin-left:35px;">Digested pGOPs 83 with SpeI and BbsI for test cut</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1410" data-index="708" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1411" data-index="709" style="margin-left:0;">(Marisa) Midiprep three of both T40 and BW363</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1412" data-index="710" style="margin-left:0;">(Marisa) Grew up pGOP 82</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1413" data-index="711" style="margin-left:0;">(Jeffrey) Grew up pGOP 83-14</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1414" data-index="712" style="margin-left:0;">(Jeffrey) took OD 600 of the interlab and diluted cells down to .02</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1415" data-index="713" style="margin-left:0;">(Will) PCR'ed the pCMV fro the iGEM registry</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1416" data-index="714" style="margin-left:0;">(Will) Gibsoned and transformed the CMV PCR product into pGEX</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1417" data-index="715" style="margin-left:0;">(Marisa) made 96 well plate for InterLab Study</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1418" data-index="716" style="margin-left:35px;">250 uL DPBS and 2uL cells per sample</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1419" data-index="717" style="margin-left:0;">(Kami Rachel) prepared HEK plates for transfection at 200,000 cells </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1420" data-index="718" style="margin-left:0;">(Marisa) Cell stock pGOP 83</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1421" data-index="719" style="margin-left:35px;">Grew up BW 1945, 1946, 1947, and 1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1422" data-index="720" style="margin-left:0;">(Will) Miniprep pGOP 82 ad 83</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1423" data-index="721" style="margin-left:0;">(Jeffrey) Picked colonies from GFP and BFP under igem CMV</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1424" data-index="722" style="margin-left:0;">(Jeffrey) Transformed and plated interlab bacteria </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1425" data-index="723" style="margin-left:0;">(Kami Rachel Marisa) made trasfection DNA mixes and transfected the HEK cells we plated yesterday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1426" data-index="724" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1427-item" data-formattable="true" data-index="725" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_145414238.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vyJM6utqStHPDJT7MEzr8eY3bZeoeR49FL0vgqqu-IMG_20160823_145414238.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1427" data-index="725">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1429-item" data-formattable="true" data-index="726" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_145437000_HDR.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="4VedDMnb5xw6XvKMWr7qj2Dl129lUE7JQ2ROGsqS-IMG_20160823_145437000_HDR.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1429" data-index="726">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1431-item" data-formattable="true" data-index="727" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_150756390_HDR.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="lhWTUAThFdvzBtPLxL88jCw7IwPVUZXSr1vTdNae-IMG_20160823_150756390_HDR.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1431" data-index="727">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1433-item" data-formattable="true" data-index="728" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_151151878_HDR.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vm8AcKbwsufChsrE7iBspYO8q3UUflLVBe2QMWBt-IMG_20160823_151151878_HDR.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1433" data-index="728">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1435" data-index="729" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1436-item" data-formattable="true" data-index="730" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.39.05.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="YVfhCtdgMu02dQlK1NY3dksXRMvpOwyt8oPHNBTi-Screen_Shot_2016-10-10_at_11.39.05.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1436" data-index="730">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1438" data-index="731" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1439-item" data-formattable="true" data-index="732" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.39.27.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JJO4oHKRdXhjb7H69jw2HneiAB5FbQi6OZOJId95-Screen_Shot_2016-10-10_at_11.39.27.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1439" data-index="732">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1441-item" data-formattable="true" data-index="733" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.39.37.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="uKtZCit3zbjRLztxqc58kSxSb6TqoKNUazpqyLmH-Screen_Shot_2016-10-10_at_11.39.37.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1441" data-index="733">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1443" data-index="734" style="margin-left:0;">Added drugs to inducible cells 2hrs after transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1444" data-index="735" style="margin-left:35px;">Drugs are located in pink box labeled "Small molecules"</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1445" data-index="736" style="margin-left:70px;">Rapalog= AC heterodimerizer (1000x)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1446" data-index="737" style="margin-left:70px;">Absistic Acid (1000x)- vortex before use to get rid of percipitant</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1447" data-index="738" style="margin-left:70px;">4OHT (1000x)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/24</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1448" data-index="739" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1449" data-index="740" style="margin-left:0;">(Marisa) Miniprepped and nanodropped pCIR 4, pBex 16 pBEX 19, CMV BFP-1, CMV GFP-1/2, 1945, 1946, 1947, 1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1450" data-index="741" style="margin-left:35px;">Grew up Bex 16 and Bex 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1451" data-index="742" style="margin-left:0;">(Jeffrey) Digested 1945, 1946, 1947, and 1948 with BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1452" data-index="743" style="margin-left:35px;">Grew up pPV 1/5/6/7/8, 1942, 1943, 1944, and 1716</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1453" data-index="744" style="margin-left:0;">(Marisa) Grew up pCir 4 (x2), DC 56, BW 390, and BW 391</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1454" data-index="745" style="margin-left:35px;">Made LB + Carb </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1455" data-index="746" style="margin-left:0;">(Rachel and Kami) Ran FACS</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1456" data-index="747" style="margin-left:0;">(Kami) split HEK293 and stable line cells into big flasks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1457" data-index="748" style="margin-left:0;">(Marisa) miniprep pPV 1, 5, 6, 7, 8, pBEX16, 19(x3), BW1942, BW1943, BW1944, BW1716</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1458" data-index="749" style="margin-left:35px;"> midipreped DC56, BW390, BW391, pCir4(x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1459" data-index="750" style="margin-left:0;">(Will) cut for gel extraction 1916, 1945, 1947, 1948 with BBs1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1460" data-index="751" style="margin-left:35px;">gel extracted those as well</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1461" data-index="752" style="margin-left:0;">(Will) grew up iGEM CMV for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1462" data-index="753" style="margin-left:0;">(Rachel) annealed the mutated oligos</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/26</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1463" data-index="754" style="margin-left:0;">(Jeffrey and Marisa) Midiprep BW 2286, BW 2287, and pCir 4 (x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1464" data-index="755" style="margin-left:35px;">Cell stock CMV GFP-1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1465" data-index="756" style="margin-left:0;">(Rachel) picked two colonies for pGOP 101-150</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1466" data-index="757" style="margin-left:0;">(Will) Gel extracted pBEX 16 and 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1467" data-index="758" style="margin-left:70px;">pBEX 16 digested with AscI and NheI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1468" data-index="759" style="margin-left:70px;">pBEX 19 digested with AscI and NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1469" data-index="760" style="margin-left:35px;">PCR overhang extension with sequences 37 and 56</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1470" data-index="761" style="margin-left:35px;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1471" data-index="762" style="margin-left:0;">(Kami, WIll, Rachel) Performed minipreps for Mismatches and additional multimerized operators pGOP 101-150</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1472" data-index="763" style="margin-left:0;">(Will) Test Cut for Mismatches </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1473" data-index="764" style="margin-left:0;">(Jeffrey) Attempted overhang extension PCR ... Failed</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1474" data-index="765" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) Attempted overhang extension PCR ... Failed</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1475" data-index="766" style="margin-left:0;">(Jeffrey) Completed Interlab Study</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1476" data-index="767" style="margin-left:0;">(Marisa) PCR GEX plasmids for CAM transfer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1477" data-index="768" style="margin-left:0;">(Will) Gel extracted PCR </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/31</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1478" data-index="769" style="margin-left:0;">(Marisa) Digested pGOP 109, 110, 112, 114, 134,l 135, 136, 138</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1479" data-index="770" style="margin-left:0;">(Marisa) Grew up pGEX 7, pGEX 8, pGPX1, </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1480" data-index="771" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) Attempted overhang extension PCR ... Failed</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1481" data-index="772" style="margin-left:0;">(Jeffrey) Test Cut <span style="color:#344043"><span style="background-color:#FFFFFF">109, 110, 112, 114, 134,l 135, 136, 138</span></span></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1482" data-index="773" style="margin-left:0;">(will) pcr mruby and bfp to drop into pgops</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1483" data-index="774" style="margin-left:0;">(Jeffrey) MInipreped pgop 80-90 for multiplecolors , bexs for circuits, gexs f</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1484" data-index="775" style="margin-left:0;">(Marisa) Passaged HEK cells </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1485" data-index="776" style="margin-left:35px;">Made 2L of LB Broth, 2L of LB+Carb broth, and 2L of LB + Cam agar for plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1486" data-index="777" style="margin-left:35px;">Ligated and transformed pGEX 3, 5, 8, 10, 15, and 19 into Cam (with BW 1721 -n330 backbone)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1487" data-index="778" style="margin-left:35px;">Grew up T4o (x2), BW 363, GEX 5, GEX 15, pCir 4 (x2), and GPX 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1488" data-index="779" style="margin-left:0;">(Rachel) autoclaved epi tubes and tips</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1489" data-index="780" style="margin-left:35px;">Ligated and transformed BW 1945-1949</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1490" data-index="781" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1491" data-index="782" style="margin-left:0;">(Marisa) Made LB + Cam plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1492" data-index="783" style="margin-left:0;">(Jeffrey) Resuspended cells grown for midiprop and stored in the cold room</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1493" data-index="784" style="margin-left:0;">(Rachel) Prepared and ran cells for FACS</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1494" data-index="785" style="margin-left:0;">(Will) Annealed oligos for multimerized pGOP 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1495" data-index="786" style="margin-left:0;">(Jeffrey) transformed mismatched pGOP 101-150</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1496" data-index="787" style="margin-left:0;">(Marisa and Jeffrey) grew up GEX 3, 5, 8, 10, 15, 19, BW 1721, 1945, 1946, 1947, 1948, and GOP 29</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/4</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1497" data-index="788" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) picked and grew up colonies from pGOP101-150</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1498" data-index="789" style="margin-left:35px;">miniprep of GEX 3, 5, 8, 10, 15, 19, BW 1721, 1945, 1946, 1947, 1948, and GOP 29</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1499" data-index="790" style="margin-left:35px;">Digested BW1945, 1946, 1947, 1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1500" data-index="791" style="margin-left:0;">(Marisa) Cell stock GOP 110, 112, and 114</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1501" data-index="792" style="margin-left:35px;">Digested GOP 29(MOP) with EcoRI and PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1502" data-index="793" style="margin-left:35px;">PCR GEX 3, 5, 8, 10, 15, 19 with PrA6 and PrA6r</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1503" data-index="794" style="margin-left:35px;">PCR clean up BW 1945, 1946, 1947, 1948 and MOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1504" data-index="795" style="margin-left:35px;">Made and ran gel for GEX 3, 5, 8, 10, 15, 19 and BW1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1505" data-index="796" style="margin-left:35px;">(with Kami) gel extracted GEX 3, 5, 8, 10, 15, 19, and BW 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1506" data-index="797" style="margin-left:35px;">Digested GEX 3, 5, 8, 10, 15, 19 and BW 1721 with EcoRI and PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1507" data-index="798" style="margin-left:35px;">PCR clean up GEX 3, 5, 8, 10, 15, 19, and BW 1721</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1508" data-index="799" style="margin-left:0;">(Marisa) Cell stock GOP 101-103; 105-108; 111; 115-134; and 146</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1509" data-index="800" style="margin-left:35px;">Miniprep pGOP 101-103; 105;108; 111; and 115-122</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1510" data-index="801" style="margin-left:35px;">Ligate and transformed pGEX 3, 5, 8, 10, 15, and 19 into pSB1C3 backbone</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1511" data-index="802" style="margin-left:0;">(Will) Miniprep pGOP 123-134 and 146</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1512" data-index="803" style="margin-left:0;">(Kami Rachel) seeded 8 48 well plates at 100,000cells for transfection on wednesday</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1513" data-index="804" style="margin-left:0;">(Marisa) picked two colonies of GEX 3, 5, 8, 10, 15, and 19 (now termed 103, 105, 108, 110, 115, and 119) from transformation 9/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1514" data-index="805" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) Performed overhang extension pcr to build the decoder for circuit 2</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1515" data-index="806" style="margin-left:0;">(Jeffrey) transformed the 18T entry vectors for circuit 5</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1516" data-index="807" style="margin-left:0;">(Marisa) miniprep GEXs 103, 105, 108, 110, 115, and 119</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1517" data-index="808" style="margin-left:35px;">Test cut with AseI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1518" data-index="809" style="margin-left:35px;">picked two colonies for 18T-terminator circuits </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1519" data-index="810" style="margin-left:0;">(Rachel, Marisa and Kami) transfected HEKcells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1520" data-index="811" style="margin-left:0;">(kami Marisa) passaged HEK cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1521" data-index="812" style="margin-left:0;">(jeffrey) performed a gibson to build circuit 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1522" data-index="813" style="margin-left:0;">(jeffrey) test cuts for 18T entry vecotrs for circuit 5 indicated low digestion efficeincy and the circuits needs to be retransformed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1523" data-index="814" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1524" data-index="815" style="margin-left:0;"><b>FRIDAY 9/9</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1525" data-index="816" style="margin-left:0;">(Will) Ligated and transformed GEX 21-40 and GOP 151-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1526" data-index="817" style="margin-left:0;">(Will and Marisa) Grew up 3 colonies for GEX 21-40 and GOP 151-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1527" data-index="818" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1528" data-index="819" style="margin-left:0;"><b>Saturday 9/10</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1529" data-index="820" style="margin-left:0;">(Will Jeffrey Marisa) Miniprepped <span style="color:#344043"><span style="background-color:#FFFFFF">GEX 21-40 and GOP 151-168</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1530" data-index="821" style="margin-left:0;">(Marisa) PCR GEX 3, 5, 8, 10, 15, 19, and BW 1721 with PrA6 and PrA6r (previous GEX 103, 105 108, 110, 115, 119 were not successful)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1531" data-index="822" style="margin-left:0;">(Marisa and Will) gel extracted GEX 3, 5, 8, 10, 15, and 19 and BW 1721 (~400); will be insert for GEX 103, 105, 108, 110, 115, and 119</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1532" data-index="823" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1533" data-index="824" style="margin-left:0;">(Marisa) Digested GEX 103, 105, 108, 110, 115, and 119 and BW 1721 inserts with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1534" data-index="825" style="margin-left:0;">(Jeffrey) Picked colonies for 18T entry vectors and miniprepred circuit 2 for test cuts</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1535" data-index="826" style="margin-left:0;">(Will) Test cut <span style="color:#344043"><span style="background-color:#FFFFFF">GEX 21-40 and GOP 151-168</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1536" data-index="827" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1537" data-index="828" style="margin-left:0;">(Marisa) picked four colonies for 18T g1, g17, g3, g8, and g13</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1538" data-index="829" style="margin-left:35px;">Digested and test-cut 18T g1, g17, g3, g8, and g13 colonies 1 and 2 with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1539" data-index="830" style="margin-left:0;">(Marisa) Ligated and transformed inserts for GEX 3, 5, 8, 10, 15, and 19 into pSB1C3 backbone to create GEX 103, 105, 108, 110, 115, and 119 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1540" data-index="831" style="margin-left:0;">(Kami) made 8 48 well plates with HEK cells at 100,000 cells/ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1541" data-index="832" style="margin-left:0;">(kami) retransformed pGOP7 because it looked wierd when we transfected it. so going to pick one colony from this transformation to try to start fresh. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1542" data-index="833" style="margin-left:0;">(Marisa) Made LB + Cam broth </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1543" data-index="834" style="margin-left:35px;">Grew up pSB1C3, GOP 35, and GPX2 for midipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1544" data-index="835" style="margin-left:35px;">Grew up pSB1C3 and GOP 45 (x2) for minipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1545" data-index="836" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1546" data-index="837" style="margin-left:0;">(Marisa) picked two colonies for GEX 103, 105, 108, 110, 115, and 119</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1547" data-index="838" style="margin-left:35px;">Midiprep pSB1C3, pGPX2, pGOP35</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1548" data-index="839" style="margin-left:35px;">Miniprep GOP45 (x2), SB1C3, and g17 18T-1/2/3/4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1549" data-index="840" style="margin-left:35px;">made master mixes for transfection to take place on 9/14</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1550" data-index="841" style="margin-left:35px;">picked and grew up a colony from GOP7 re-transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1551" data-index="842" style="margin-left:35px;">Added Carbacillin to plain LB Broth to make LB+Carb broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1552" data-index="843" style="margin-left:35px;">Grew up Cir4 (x2), SB1C3 (x2), GEX5, GEX 15, GPX2, T40 (x2), and BW471 </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1553" data-index="844" style="margin-left:0;">(Marisa) miniprepped pGEX 103-1/2, 105-1/2, 108-1/2, 110-1/2, 115-1/2, and 119-1/2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1554" data-index="845" style="margin-left:35px;">Test cut GEXs with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1555" data-index="846" style="margin-left:35px;">Test Cut g17 18T with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1556" data-index="847" style="margin-left:0;">(Marisa and Jeffrey) made 2L of plain LB broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1557" data-index="848" style="margin-left:0;">(Jeffrey) midiprep pSB1C3 (x2), T40(x2), and pCir4(x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1558" data-index="849" style="margin-left:0;">(Kami Rachel) transfected Hek cells. with pGOPs diluted to 5ng/ul (not 50ng/ul) and add a bigger volume to the transfection mix. </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1559-item" data-formattable="true" data-index="850" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.43.39.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JEeap2VgHjzbtoJ48RyQsp5aweyRHe3V5g8lVTUC-Screen_Shot_2016-09-15_at_01.43.39.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1559" data-index="850">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1561-item" data-formattable="true" data-index="851" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.43.46.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="7elqO9a2oEa1WuI28OtvtR3luaswiUZ95EB6cI2l-Screen_Shot_2016-09-15_at_01.43.46.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1561" data-index="851">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1563-item" data-formattable="true" data-index="852" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.44.02.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Gevqn5XtZwrpeRO5ANTKcMNUtU8zZKFxAl2QxYrG-Screen_Shot_2016-09-15_at_01.44.02.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1563" data-index="852">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1565-item" data-formattable="true" data-index="853" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.44.20.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="735677Efcro0ZK7rsHbSwcRzzxewxT5tswpeTaPP-Screen_Shot_2016-09-15_at_01.44.20.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1565" data-index="853">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1567-item" data-formattable="true" data-index="854" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.44.29.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Lc9mxtOSzkCpHAMy7XbTlzIIYwaRoGtAXbCxh9bY-Screen_Shot_2016-09-15_at_01.44.29.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1567" data-index="854">

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</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1569-item" data-formattable="true" data-index="855" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.45.21.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="b56gbrHOLk79LJnXldGESIxsg5XryN1VJGKce8ND-Screen_Shot_2016-09-15_at_01.45.21.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1569" data-index="855">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1571" data-index="856" style="margin-left:0;">(Marisa) Miniprep GEX 103-3/4/5/6, 105-3/4/5/6, 108-3/4/5/6, 110-3/4/5/6, 115-3/4/5/6, 119-3/4/5/6</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1572" data-index="857" style="margin-left:35px;">Digest and test cut GEXs with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1573" data-index="858" style="margin-left:0;">(Marisa and Kami) passaged stable line HEK cells (Wild Type, 2578, and 2579) 1:20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1574" data-index="859" style="margin-left:0;">(Jeffrey) Minipreped 1945-1948</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1575" data-index="860" style="margin-left:0;">(Kami) Miniprep pGOP 159-1/2/3, 160-1/2/3, 169-1/2/3, and 170-1/2/3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1576" data-index="861" style="margin-left:0;">(Will) Test cut pGOP 159, 160, 169, and 170</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1577" data-index="862" style="margin-left:0;">(jeffrey) ligated and transformed BEX34-38</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1578" data-index="863" style="margin-left:0;">(Marisa and Jeffrey) picked three colonies of GOP 151-158 and 161-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1579" data-index="864" style="margin-left:0;">(Jeffrey) Picked colonies for BEX37 Redigested 1945,1946,1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1580" data-index="865" style="margin-left:0;">(Jeffrey) Religated and transformed BEX 34,35,36,38</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1581" data-index="866" style="margin-left:0;">(Marisa) Miniprep colonies 1,2, and 3 of GOP 151-158 and 161-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1582" data-index="867" style="margin-left:0;">(Jeffrey) PIcked colonies for BEX 34-38</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1583" data-index="868" style="margin-left:0;">(Kami) Made 6 hek plates at 100,000 (NOT USED)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1584" data-index="869" style="margin-left:0;">(Will) Test cut the miniprepped GOPs 151-168</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1585" data-index="870" style="margin-left:0;">(Marisa) Cell stock BEX 30, GOP 159, 160, 169</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1586" data-index="871" style="margin-left:0;">(Marisa) made 2L of plain LB broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1587" data-index="872" style="margin-left:0;">(Marisa) Diluted GOP 81-90 to 25 ng/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1588" data-index="873" style="margin-left:0;">(jeffrey) Minipreppred bex 34-38 and test cut</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1589" data-index="874" style="margin-left:0;">(Jeffrey) Digested BEX 1,6,15,20, 30,31,32,33</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1590" data-index="875" style="margin-left:0;">(Jeffrey) digested parts vectors with bbsi for 3 input circuit</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1591" data-index="876" style="margin-left:0;">(Will) Sent in colony 1 of GOPs 151-168 that test cut correctly</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1592" data-index="877" style="margin-left:0;">(Marisa and Rachel) Made LB agar (x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1593" data-index="878" style="margin-left:0;">(Marisa) Cell stock pGOP 159, 160, and 169</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1594" data-index="879" style="margin-left:0;">(Marisa and Kami) Ligated GOP 153, 154, 157, 163, 165, 166, 168, 170, 162, and 167 with respective oigos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1595" data-index="880" style="margin-left:0;">(rachel) transformed the pGOPs ligated above</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1596" data-index="881" style="margin-left:0;">(Kami) Made LB + Cam plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1597" data-index="882" style="margin-left:0;">(Marisa) Made LB + Carb plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1598" data-index="883" style="margin-left:0;">(Jeffrey) Gibson Cir2 and Cir 5 using BEX parts vectors</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1599" data-index="884" style="margin-left:0;">(Kami) picked 4 colonies for GOP 153, 154, 157, 162, 163, 165, 166, 167, 168, 170</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1600" data-index="885" style="margin-left:0;">(Jeffrey) Cell stock GOP 151, 152, 155, 156, 158, 161, 164 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1601" data-index="886" style="margin-left:35px;">Miniprepped colonies 5-8 of GOP <span style="color:#344043"><span style="background-color:#FFFFFF">153, 154, 157, 162, 163, 165, 166, 167, 168, 170</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1602" data-index="887" style="margin-left:35px;">Test cut GOPs with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1603" data-index="888" style="margin-left:35px;">Digested BEX 34-37 with AscI and NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1604" data-index="889" style="margin-left:35px;">Digested BEX 38 with AscI and NheI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1605" data-index="890" style="margin-left:0;">(Kami) passaged HEK cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1606" data-index="891" style="margin-left:0;">(Will) Sent in colony two of GOPs 151-168 that did not sequence properly</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1607" data-index="892" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1608" data-index="893" style="margin-left:0;">Cell stocked pGOP 154, </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1609" data-index="894" style="margin-left:0;">(Rachel) Ligated and transformed 1721 insert into MOP (GOP with biobrick cut out)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1610" data-index="895" style="margin-left:0;">(Jeffrey) Gibson Circuit 2 and 5</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/26</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1611" data-index="896" style="margin-left:0;">(Marisa) miniprepped four colonies of pGOP 104, 109, 135, 137, 144, 149, and 150</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1612" data-index="897" style="margin-left:35px;">Test cut GOP (x3) of 91-100, GOP (x4) 104, 109, 135, 137, 144, 149, 150, 153, 154, 157, 162, 163, 165, 166, 167, 168, 170, and GOP (x3) 113, 135, 136, 138, 142, with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1613" data-index="898" style="margin-left:0;">(Marisa) Transformed Circuit 2 and Circuit 5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1614" data-index="899" style="margin-left:0;">(Marisa) picked four colonies of MOP + 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1615" data-index="900" style="margin-left:0;">(Will) Sent in GOP151 and 163 for sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1616" data-index="901" style="margin-left:0;">(Kami) made HEK plates</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1617" data-index="902" style="margin-left:0;">(Marisa) PCR pGEX 3, 8, 15, and 19 and BW 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1618" data-index="903" style="margin-left:0;">(Marisa and Will) Gel extracted pGEX 3, 8, 15, 19 and BW 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1619" data-index="904" style="margin-left:0;">(Will) Digested GEX 3, 8, 15, 19 and BW 1721 with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1620" data-index="905" style="margin-left:35px;">PCR cleanup of GEXs and BW1721 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1621" data-index="906" style="margin-left:0;">(Rachel) Ligated GEX 3, 8, 15, 19, and BW 1721 with MOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1622" data-index="907" style="margin-left:0;">(Marisa) Transformed GEX 3, 8, 15, 19 and BW 1721 (to GEX 103, 108, 115, and 119)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1623" data-index="908" style="margin-left:0;">(Will) Cell stocked pGOP 151 and 163</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1624" data-index="909" style="margin-left:0;">(Marisa) Test cut GOP 162, 163, and 167 colonies 5-8 with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1625" data-index="910" style="margin-left:0;">(Marisa) Cell stock 91-100, 104, 109, 113, 136, 137, 138, 142, 144, 149, 150, 153, and 157</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1626" data-index="911" style="margin-left:0;">(Marisa) miniprep GOP 112, 114, 135, </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1627" data-index="912" style="margin-left:0;">(Marisa) Replated Circuit 2 and 5</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1628" data-index="913" style="margin-left:0;">(Marisa) Cell stock pGOP 151 and 163</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1629" data-index="914" style="margin-left:0;">(Marisa) Replated Circuit 2 and Circuit 5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1630" data-index="915" style="margin-left:0;">(Marisa and Rachel) ligated and transformed pGOP 142, 162, and 167</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1631" data-index="916" style="margin-left:0;">(Marisa) picked 5 colonies of pGOP 167</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1632" data-index="917" style="margin-left:0;">(Kami) made 11 hek plates at 100,000cells/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1633" data-index="918" style="margin-left:0;">(kami)Diluted pGOPs to 25ng/ul</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 10/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1634" data-index="919" style="margin-left:0;">(Marisa) Miniprep and test cut pGOP 167-1/2/3/4/5 with BbsI and SpeI</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 10/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1635" data-index="920" style="margin-left:0;">(Kami Rachel) Diluted DNA for transfection. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1636" data-index="921" style="margin-left:0;">(Kami Rachel) Transfected hek cells with all the multimerized and mutated pGOPs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1637" data-index="922" style="margin-left:35px;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1638-item" data-formattable="true" data-index="923" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.03.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="pPGDrwkHDdZI8My8HLfC7fuIGWvUanttaSvsxIs3-Screen_Shot_2016-10-10_at_11.34.03.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1638" data-index="923">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1640" data-index="924" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1641-item" data-formattable="true" data-index="925" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.15.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="yB64ra9qxRfhK1xwX5bjOvBPrl5pb3eM5QlMfP5e-Screen_Shot_2016-10-10_at_11.34.15.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1641" data-index="925">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1643" data-index="926" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1644-item" data-formattable="true" data-index="927" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.25.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="MQFs5Y7UrPnIGabfCi1yaDFzk2vyELbrTFjkVilu-Screen_Shot_2016-10-10_at_11.34.25.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1644" data-index="927">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1646" data-index="928" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1647-item" data-formattable="true" data-index="929" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.37.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="i5ZQqz1LHKinEmYksDWlwIN4BSGJAsaUAPQ63dAA-Screen_Shot_2016-10-10_at_11.34.37.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1647" data-index="929">

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</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 10/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1649" data-index="930" style="margin-left:0;">(kami) made 7 hek plates at 100,000 for transfection on sunday and 1 hek plate at 200,000 cells/ml for transfection on saturday with WPI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1650" data-index="931" style="margin-left:0;">(Rachel) prepared the transfection DNA mix for WPI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1651-item" data-formattable="true" data-index="932" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.30.05.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="SMbeGz3C71A7aXcgKue8BXssQiBDMkSFA5CRbx7B-Screen_Shot_2016-10-10_at_11.30.05.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1651" data-index="932">

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</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1653" data-index="933" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1654" data-index="934" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 10/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1655" data-index="935" style="margin-left:0;">(Kami + Rachel) transfected cells for WPI testing the and/nor circuit</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1656" data-index="936" style="margin-left:0;">(Kami + Rachel) prepared all the DNA mixes for transfection tomorrow</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1657" data-index="937" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 10/9</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1658" data-index="938" style="margin-left:0;">(Kami) Transfected HEK cells with screen of mutated p GOPs, CMV compared to single and triple multimerized, and 4 analog circuits</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1659" data-index="939" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1660-item" data-formattable="true" data-index="940" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.32.13.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="35zz4Sc5nei3ggaIX5VH7t2s3wXjf8oXDAfQtvP9-Screen_Shot_2016-10-10_at_11.32.13.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1660" data-index="940">

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-<title>iGEM Notebook · Benchling</title>
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-<body><div><div class="printable-container"><div><div class="printable-page-container"><div class="noteEditor mediocre" tabindex="-1"><div><noscript></noscript><noscript></noscript><noscript></noscript></div><div class="noteEditor-container"><div class="u-cursor-text noteEditor-catcher u-disable-margin-collapse dnd-drop-container"><div class="noteEditor-contentWrapper"><h1 class="mediocre-titleEditor"><div class="editable selectable is-locked" contenteditable="false" data-allow-multiline="false" data-cid="notetitleeditor" data-index="0">iGEM Notebook</div></h1><div><div><i class="faded">Made with Benchling</i></div><div><b>Project:</b> Protocols</div><div><b>Authors:</b> Kamila Drezek</div><div><b>Dates:</b> 2016-05-19 to 2016-10-09</div></div><div class="editor" data-editor="notes"><div class="hiddenFocusEditable js-focusBearer" contenteditable="true" tabindex="-1"></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 5/19/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part637" data-index="0" style="margin-left:0;">Preparing Reagants</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 5/21/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part638" data-index="1" style="margin-left:35px;">LB Broth (1L)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part639" data-index="2" style="margin-left:70px;">20 grams per liter</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part640" data-index="3" style="margin-left:35px;">LB Agar (1L)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part641" data-index="4" style="margin-left:70px;">40  grams per liter</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part642" data-index="5" style="margin-left:35px;">Prepare two liter containers of each- one per antibiotic (Cam and Carb)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part643" data-index="6" style="margin-left:35px;">Autoclave for 1 hour</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part644" data-index="7" style="margin-left:0;">Preparing chloramphenicol stock (1000x)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part645" data-index="8" style="margin-left:35px;">Final concentration of 1x chloramphenicol should be 25 ug/ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part646" data-index="9" style="margin-left:35px;">Stock concentration of 1000x chloramphenicol should be 25 mg/ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part647" data-index="10" style="margin-left:35px;">Prepare 50 ml of stock to aliquot into microcentrifuge tubes by measuring 1.25 g of chloramphenicol and ~50ml of 200 proof ethanol in a 50 ml Falcon 	</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part648" data-index="11" style="margin-left:35px;">Filter chloramphenicol through 20 um syringe filter</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part649" data-index="12" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part650" data-index="13" style="margin-left:0;">Preparing Plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part651" data-index="14" style="margin-left:35px;">20ml agar per plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part652" data-index="15" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part653" data-index="16" style="margin-left:0;">Innoculate Vector 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part654" data-index="17" style="margin-left:35px;">5 ml of LB+carb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part655" data-index="18" style="margin-left:35px;">Stab cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part656" data-index="19" style="margin-left:35px;">Grow overnight at 37C shaker</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 5/22/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part657" data-index="20" style="margin-left:35px;">Preparing Cell stock of 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part658" data-index="21" style="margin-left:70px;">Box Name- iGEM 2016 Source Box</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part659" data-index="22" style="margin-left:70px;">Freezer Number- 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part660" data-index="23" style="margin-left:70px;">Shelf- 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part661" data-index="24" style="margin-left:35px;">Vector 1721 is miniprepped</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part662" data-index="25" style="margin-left:70px;">See protocol sheet</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part663" data-index="26" style="margin-left:35px;">Vector 1721 is digested (300ul digest)(1-2hrs)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part664" data-index="27" style="margin-left:70px;">24000 ng digestion of DNA </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part665" data-index="28" style="margin-left:105px;">30 ul buffer (cold room)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part666" data-index="29" style="margin-left:105px;">9ul of BpsI (-80C, small green box)(incubation in 37C)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part667" data-index="30" style="margin-left:105px;">101 ul of water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part668" data-index="31" style="margin-left:35px;">Digestion Clean-Up of Vector 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part669" data-index="32" style="margin-left:70px;">See PCR cleanup protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part670" data-index="33" style="margin-left:70px;">Note- vector was eluted in 50 ul of EB not 30 ul. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part671" data-index="34" style="margin-left:35px;">Dilution of Oligos (ideal final concentration is 100uM)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part672" data-index="35" style="margin-left:70px;">Short Cut- </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part673" data-index="36" style="margin-left:105px;">300 ul of TE for values ~25- ~35 nmol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part674" data-index="37" style="margin-left:105px;">200 ul of TE for value  ~15 - ~24 nmol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part675" data-index="38" style="margin-left:70px;">Note- Oligo 12, 13, 14, 17, 18, and 20 was vortex without liquid initially</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part676" data-index="39" style="margin-left:35px;">Annealing of pGEX oligos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part677" data-index="40" style="margin-left:70px;">See protocol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part678" data-index="41" style="margin-left:70px;">Annealing time- 2 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part679" data-index="42" style="margin-left:70px;">dilute oligos to 1ng/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part680" data-index="43" style="margin-left:35px;">Ligation of 1721 and insert</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part681" data-index="44" style="margin-left:70px;">Note- diluted annealed oligos named- pGEX#A where # is the number of the construct</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part682" data-index="45" style="margin-left:70px;">24 reactions</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part683" data-index="46" style="margin-left:105px;">139.2 ul of water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part684" data-index="47" style="margin-left:105px;">24 ul of T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part685" data-index="48" style="margin-left:105px;">24 ul of  T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part686" data-index="49" style="margin-left:105px;">26.4 ul of Vector</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part687" data-index="50" style="margin-left:35px;">Transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part688" data-index="51" style="margin-left:70px;">See protocol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part689" data-index="52" style="margin-left:70px;">Plates put in incubator at 7:09 PM- Desired pick up time is 10:09</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 5/23/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part690" data-index="53" style="margin-left:70px;">pGEX plates were pulled from incubator at 10:15</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part691" data-index="54" style="margin-left:105px;">Growth and density of all samples exceeded that of the negative control</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 5/24/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part692" data-index="55" style="margin-left:70px;">Picked two colonies per plate and grew in 4ml of LB+carb in 37C shaker. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part693" data-index="56" style="margin-left:70px;">Created stab plate for each colony. </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 5/25/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part694" data-index="57" style="margin-left:70px;">Minipreping colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part695" data-index="58" style="margin-left:105px;">Notes: During the miniprep samples from colony 10 were contaminated</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part696" data-index="59" style="margin-left:70px;">Test Cut </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part697" data-index="60" style="margin-left:105px;">4 ul DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part698" data-index="61" style="margin-left:105px;">1 ul 2.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part699" data-index="62" style="margin-left:105px;">.25 of ul each enzyme (Bsp1 and Nco1)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part700" data-index="63" style="margin-left:105px;">4.5 ul water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part701" data-index="64" style="margin-left:105px;">Run digestion for at least 15 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part702" data-index="65" style="margin-left:70px;">Test Cut Results</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part703" data-index="66" style="margin-left:105px;">All colonies appeared with proper bands at 1.1 kb and 4.2kb and no bands at 440 bp. We decided to send only the first colony from each set in for sequencing</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part704-item" data-formattable="true" data-index="67" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">pGEX1-1 to pGEX12-2 test cuts</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="XZPsOM1Dwmy0jxyx52av0qGrK7hOK5q9mzmggCU7-IMG_8763.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part704" data-index="67">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part706-item" data-formattable="true" data-index="68" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">pGEX13-1 to pGEX20-2 test cuts</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="SOkSYjGFUBFO3eDx6yVdJNsaCyR1fCluH49hD24N-IMG_8764.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part706" data-index="68">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 5/26/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part708" data-index="69" style="margin-left:35px;">Sequencing results are in</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part709" data-index="70" style="margin-left:70px;">All sequences with the exceptions of pGEX1-1, pGEX14-1, and pGEX 15-1 were successful.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part710" data-index="71" style="margin-left:105px;">All sequences reamining sequences were then grown up for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part711" data-index="72" style="margin-left:140px;">Note: While pGEX 6-1 sequenced properly the tube containing its reference appeared to have been contaminated and pGEX 6-2 will be sent in for sequencing to reverify</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part712" data-index="73" style="margin-left:35px;">Sequencing sent in</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part713" data-index="74" style="margin-left:70px;">pGEX: 1-2, 6-2, 14-1, 14-2, 15-1, 15-2, 10-1, 10-2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part714" data-index="75" style="margin-left:35px;">Restriction digest of 2490 and DC16 with MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part715" data-index="76" style="margin-left:70px;">5ul buffer3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part716" data-index="77" style="margin-left:70px;">6ul DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part717" data-index="78" style="margin-left:70px;">1.5ul Nru1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part718" data-index="79" style="margin-left:70px;">1.5ul Mlu1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part719" data-index="80" style="margin-left:70px;">36ul water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part720" data-index="81" style="margin-left:70px;">Prerequisites to subvectors which will be used to create one plasmid with Cre and both Flps </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part721" data-index="82" style="margin-left:105px;">Subvectors to be made have homology with each other, allowing for easy gibson assembly with no PCR (promoters and ERT2 - modified estrogen receptor - have repeat regions)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part722" data-index="83" style="margin-left:70px;">Digestion of DC16 yielded 3 bands rather than the expected 2.  Culprit may be unreliable cell stock.</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part723-item" data-formattable="true" data-index="84" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">DC16 2490 1st digestion.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JghBH5DpmwrD1qFjKyUVXR7yoGrbaeBjMa8Xmo5F-DC16_2490_1st_digestion.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part723" data-index="84">We were supposed to have:                                         DC16 bands at 10723bp,<b><u> 1172bb</u></b>.                                                                                2490 bands at <b><u>10590bp</u></b> and 227bp.
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part725" data-index="85" style="margin-left:35px;">Ligate 2490 and DC16 to create 2534 (10ul)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part726" data-index="86" style="margin-left:70px;">See Ligation Calculator</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part727" data-index="87" style="margin-left:70px;">4.2 ul DI water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part728" data-index="88" style="margin-left:70px;">1.0 ul DC16 (insert)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part729" data-index="89" style="margin-left:70px;">2.8 ul 2490 (vector)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part730" data-index="90" style="margin-left:70px;">1 ul T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part731" data-index="91" style="margin-left:70px;">1 ul T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part732" data-index="92" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part733" data-index="93" style="margin-left:35px;">Construct 2534 and a negative control are transformed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part734" data-index="94" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part735" data-index="95" style="margin-left:35px;">Tissue Culture Room</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part736" data-index="96" style="margin-left:70px;">Prepping hood for trannsfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part737" data-index="97" style="margin-left:105px;">Remember to clean the hood down with 70%  ethanol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part738" data-index="98" style="margin-left:70px;">Aspirate all media from the plate to aid in the trypsin function</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part739" data-index="99" style="margin-left:105px;">Kidney cells are stored in T75 flask</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part740" data-index="100" style="margin-left:70px;">After aspiration perform a PBS wash</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part741" data-index="101" style="margin-left:105px;">Phosphate Buffer Solution- a salt water solution (~5ml wash)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part742" data-index="102" style="margin-left:70px;">Trypsin is used remove kidney cells from the plate.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part743" data-index="103" style="margin-left:105px;">Add 2.5ml of Trypsin to the T75</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part744" data-index="104" style="margin-left:140px;">Dont shoot directed at the cells, let the trpysin run over them.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part745" data-index="105" style="margin-left:70px;">Addition of Medium will neutralize trypsin</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part746" data-index="106" style="margin-left:105px;">Add 7.5 ml of media to plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part747" data-index="107" style="margin-left:105px;">And tritrate (Pipette up and down)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part748" data-index="108" style="margin-left:105px;">To improve cell count- spin cells down then respend them</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part749" data-index="109" style="margin-left:140px;">Spin at 300 RCF for 2-5 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part750" data-index="110" style="margin-left:140px;">To resuspend- tap the hood with the 50ml falcon tube</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part751" data-index="111" style="margin-left:140px;">Add back 10 ml of media and tritrate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part752" data-index="112" style="margin-left:70px;">Performing a cell count</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part753" data-index="113" style="margin-left:105px;">Add 10 ul of cell solution to a cell counting slides</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part754" data-index="114" style="margin-left:140px;">Divide the cell count concentration by 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part755" data-index="115" style="margin-left:140px;">Actual Cell Counts</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:175px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part756" data-index="116" style="margin-left:175px;">~2.5 Million cells per ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part757" data-index="117" style="margin-left:70px;">Magic Number for transfection:</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part758" data-index="118" style="margin-left:105px;">200,000 cells per ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part759" data-index="119" style="margin-left:105px;">For each T75- 20 ml of solution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part760" data-index="120" style="margin-left:105px;">For desired concentration of cells- </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part761" data-index="121" style="margin-left:140px;">Add 4.8 ml of cell concentrate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part762" data-index="122" style="margin-left:140px;">Add 55.2 ml of media</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part763" data-index="123" style="margin-left:105px;">Continuing the cell line</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part764" data-index="124" style="margin-left:140px;">Note: </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:175px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part765" data-index="125" style="margin-left:175px;">Split/Passage: diluting cells in the same vessel </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:140px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part766" data-index="126" style="margin-left:140px;">Split the parent cell line </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 5/27/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part767" data-index="127" style="margin-left:35px;">pGEX Cells moved to Stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part768" data-index="128" style="margin-left:70px;">1-13, 16-20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part769" data-index="129" style="margin-left:35px;">Sequencing results </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part770" data-index="130" style="margin-left:70px;">6-2 and 1-2 are perfect</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part771" data-index="131" style="margin-left:70px;">pGEX 14 ans 15 failde to sequence again</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:105px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part772" data-index="132" style="margin-left:105px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part773" data-index="133" style="margin-left:35px;">Transformation for Construct 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part774" data-index="134" style="margin-left:70px;">No significant growth in the positive- less than what appeared in the negative -&gt; failure</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part775" data-index="135" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part776" data-index="136" style="margin-left:35px;">Digest a different aliquot of DC16 at a low concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part777" data-index="137" style="margin-left:70px;">Need 4ng of DNA with DNA at 45ng/uL so need 89 uL of DNA; will do 100 ish uL digest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part778" data-index="138" style="margin-left:70px;">89 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part779" data-index="139" style="margin-left:70px;">1.5 uL MluI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part780" data-index="140" style="margin-left:70px;">1.5 uL NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part781" data-index="141" style="margin-left:70px;">10 uL Buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part782" data-index="142" style="margin-left:70px;">No water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part783" data-index="143" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part784" data-index="144" style="margin-left:35px;">Ligation of dc16 insert and 2490 vector</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part785" data-index="145" style="margin-left:70px;">2.7 ul of vector</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part786" data-index="146" style="margin-left:70px;">1.7 ul of insert</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part787" data-index="147" style="margin-left:70px;">3.5 ul of water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part788" data-index="148" style="margin-left:70px;">1 ul of T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part789" data-index="149" style="margin-left:70px;">1 ul of T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part790" data-index="150" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part791" data-index="151" style="margin-left:35px;">Digestion of pSb1A3 and pBS1C3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part792" data-index="152" style="margin-left:70px;">40 ul of DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part793" data-index="153" style="margin-left:70px;">5  ul of Cutsmart BUffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part794" data-index="154" style="margin-left:70px;">1.5 ul of EcoRI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part795" data-index="155" style="margin-left:70px;">1.5 ul of PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part796" data-index="156" style="margin-left:70px;">2 ul of Water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part797" data-index="157" style="margin-left:70px;">After digestion treat witl DnpI for 15 minutes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part798" data-index="158" style="margin-left:70px;">PCR Clean- up and elute in 30 ul of EB</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part799" data-index="159" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part800" data-index="160" style="margin-left:35px;">Gibson of pSb1A3 (2533) and pSb1C3 (2531)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part801" data-index="161" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part802" data-index="162" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">CONSTRUCT </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">NAME</span></span><span style="color:#344043"><span style="background-color:#FFFFFF"> </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">2531</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part803" data-index="163" style="margin-left:0;">Mass Vec =25
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part804" data-index="164" style="margin-left:0;">Ratio =		3
-				<span style="color:#344043"><span style="background-color:#FFFFFF">V	</span></span> <span style="color:#344043"><span style="background-color:#FFFFFF">A	</span></span> <span style="color:#344043"><span style="background-color:#FFFFFF">B	</span></span> <span style="color:#344043"><span style="background-color:#FFFFFF">C</span></span> <span style="color:#344043"><span style="background-color:#FFFFFF">	D</span></span> <span style="color:#344043"><span style="background-color:#FFFFFF">	W</span></span>
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part805" data-index="165" style="margin-left:0;">		 SIZE	2000	1200		600		0		0
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part806" data-index="166" style="margin-left:0;">		 CONC	5.6		10			10		1		1
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part807" data-index="167" style="margin-left:0;">		 VOL	4.5		4.5			2.3		0.0		0.0		-1.2
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part808" data-index="168" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">CONSTRUCT </span></span><span style="color:#344043"><span style="background-color:#FFFFFF">NAME </span></span>2533
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part809" data-index="169" style="margin-left:0;">		SIZE	2000	1200		600		0		0
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part810" data-index="170" style="margin-left:0;">		CONC	23		10			10		1		1
-</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part811" data-index="171" style="margin-left:0;">		VOL	1.1		4.5			2.3		0.0		0.0		2.2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part812" data-index="172" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part813" data-index="173" style="margin-left:35px;">Both the Gibson Assembly Transformation and the Ligation Transformation are over 10kb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part814" data-index="174" style="margin-left:70px;">See Protocol </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part815" data-index="175" style="margin-left:0;">Stable Line Integration of  2494-2496</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part816" data-index="176" style="margin-left:35px;">See Protocol for Stable Line Transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part817" data-index="177" style="margin-left:35px;">Construct 900- Piggy back transpose</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part818" data-index="178" style="margin-left:35px;">Cell status preceeding transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part819" data-index="179" style="margin-left:70px;">Low cell count ~30-40% confluences </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part820" data-index="180" style="margin-left:35px;">Thaw out 2 tubes of PEI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part821" data-index="181" style="margin-left:70px;">Use an incubator in a BL2 room</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part822" data-index="182" style="margin-left:35px;">Note: We are making 4x the number of cells than what is required</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part823" data-index="183" style="margin-left:35px;">Mixes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part824" data-index="184" style="margin-left:70px;">DNA part</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part825" data-index="185" style="margin-left:70px;">.78 ul of transposase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part826" data-index="186" style="margin-left:70px;">3.2 ul of DNA </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part827" data-index="187" style="margin-left:70px;">.15 M NaCl (3ml per tube)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part828" data-index="188" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part829" data-index="189" style="margin-left:70px;">PEI part</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part830" data-index="190" style="margin-left:70px;">2ml of PEI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part831" data-index="191" style="margin-left:70px;">.15 M NaCl ( 10.5 ml)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part832" data-index="192" style="margin-left:35px;">Add 4 ml of PEI part to DNA part</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part833" data-index="193" style="margin-left:35px;">Transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part834" data-index="194" style="margin-left:70px;">Don't pour directly onto the- shoot away from the cells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 5/28/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part835" data-index="195" style="margin-left:35px;">Create cell stock of pGEX10-1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part836" data-index="196" style="margin-left:35px;">MIniprep of 14-3/4/5 15-3/4/5 and 2534-1/2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part837" data-index="197" style="margin-left:70px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part838" data-index="198" style="margin-left:35px;">Test Cut Results</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part839-item" data-formattable="true" data-index="199" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05-26-16 pGEX14,15  and 2434 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vFOrZaGyaRhiPRTWzSMBGXdbdGvuxYHrqKdf5XTk-05-26-16_pGEX1415_and_2434_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part839" data-index="199">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part841" data-index="200" style="margin-left:35px;">pGEX 14-3, pGEX 15-5, 2534-2 =&gt; sent to sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part842" data-index="201" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part843" data-index="202" style="margin-left:35px;">Cell inspection preceeding puromycin selection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part844" data-index="203" style="margin-left:70px;">~90% confluence</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part845" data-index="204" style="margin-left:35px;">Puromycin Selection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part846" data-index="205" style="margin-left:70px;">4ul of concentrated puromycin - 2ug per ml to each flask</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part847" data-index="206" style="margin-left:35px;">Wait 10 days for total transient plasmids are diluted outs.</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 5/29/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part848" data-index="207" style="margin-left:0;">Sequencing Results</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part849" data-index="208" style="margin-left:35px;">pGEX 15-5 can be moved to cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part850" data-index="209" style="margin-left:35px;">pGEX 14-3 failed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part851" data-index="210" style="margin-left:35px;">2534 failed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part852" data-index="211" style="margin-left:0;">Sent Sequencing </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part853" data-index="212" style="margin-left:35px;">pGEX 14-4, pGEX 14-5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part854" data-index="213" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part855" data-index="214" style="margin-left:0;">Digestion</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part856" data-index="215" style="margin-left:35px;">2531 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part857" data-index="216" style="margin-left:70px;">4 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part858" data-index="217" style="margin-left:70px;">4 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part859" data-index="218" style="margin-left:70px;">1 uL CutSmart</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part860" data-index="219" style="margin-left:70px;">0.5 uL EcoRI and AgeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part861" data-index="220" style="margin-left:35px;">2533</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part862" data-index="221" style="margin-left:70px;">4 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part863" data-index="222" style="margin-left:70px;">4 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part864" data-index="223" style="margin-left:70px;">1 uL CutSmart</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part865" data-index="224" style="margin-left:70px;">0.5 uL EcoRI and AgeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part866" data-index="225" style="margin-left:35px;">DC16 midiprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part867" data-index="226" style="margin-left:70px;">1 uL DNA at 1:10 dilution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part868" data-index="227" style="margin-left:70px;">7 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part869" data-index="228" style="margin-left:70px;">1 uL Buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part870" data-index="229" style="margin-left:70px;">0.5 uL MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part871" data-index="230" style="margin-left:35px;">DC16 miniprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part872" data-index="231" style="margin-left:70px;">2 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part873" data-index="232" style="margin-left:70px;">6 uL DI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part874" data-index="233" style="margin-left:70px;">1 uL Buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part875" data-index="234" style="margin-left:70px;"><span style="color:#344043"><span style="background-color:#FFFFFF">0.5 uL MluI and NruI</span></span></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part876-item" data-formattable="true" data-index="235" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">05-27-2016 DC16 DC56 2531 test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="8U0ZRkGS72jLVot8K8KxyFpZfIuTISwDvtnvYD8O-05-27-2016_DC16_DC56_2531_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part876" data-index="235">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part878" data-index="236" style="margin-left:0;">dc16 Primers</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part879" data-index="237" style="margin-left:0;">BP0165, BP0162 reverse</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part880" data-index="238" style="margin-left:0;">BP0198, BP0176 forward</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part881" data-index="239" style="margin-left:0;">dc56 Primers</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part882" data-index="240" style="margin-left:0;">BP0165, BP0130 reverse</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part883" data-index="241" style="margin-left:0;">BP0038, BP0188 forward</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part884" data-index="242" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part885" data-index="243" style="margin-left:0;">pGEX 14-4 and pGEX14-5 moved to cell stock pending sequencing</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 5/31/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part886" data-index="244" style="margin-left:0;">Phosphorylating and Annealing Oligos for pGOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part887" data-index="245" style="margin-left:0;">Moved Construct 2531 to cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part888" data-index="246" style="margin-left:0;">Grow up more 2531 for ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part889" data-index="247" style="margin-left:35px;">Because of Chloramphenicol's effect on growth, we will grow this up for 24 hours</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part890" data-index="248" style="margin-left:0;">Oligos are at 1000x concentration- dilute to 1x (~1ng/ul)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part891" data-index="249" style="margin-left:0;">Picked more colonies from 2533 and grew up overnight</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/1/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part892" data-index="250" style="margin-left:0;">Miniprep 2531 (grown again due to low inital concentration) and 2533 (initial sequencing was incorrect)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part893" data-index="251" style="margin-left:0;">Digest 2531 with BbsI (50 uL total)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part894" data-index="252" style="margin-left:35px;">30 uL DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part895" data-index="253" style="margin-left:35px;">5 uL Buffer 2.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part896" data-index="254" style="margin-left:35px;">2 uL BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part897" data-index="255" style="margin-left:35px;">13 uL DI water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part898" data-index="256" style="margin-left:0;">Ligate oligos with 2531 to create pGOP1-20 (10uL total)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part899" data-index="257" style="margin-left:35px;">See protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part900" data-index="258" style="margin-left:35px;">0.4 uL 2531 (BbsI)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part901" data-index="259" style="margin-left:35px;">1.3 uL annealed oligos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part902" data-index="260" style="margin-left:35px;">6.4 uL DI water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part903" data-index="261" style="margin-left:35px;">1 uL T4 Ligase Buffer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part904" data-index="262" style="margin-left:35px;">1 uL T4 Ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part905" data-index="263" style="margin-left:0;">All pGOP plasmids were transformed and plated (25 ul) with Control</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part906" data-index="264" style="margin-left:0;">Test Cut 2533 with EcoR1 Age1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part907-item" data-formattable="true" data-index="265" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-01-2016 testcut of 2533.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ceUPYfY3mt1dz4j7IeklYEn5SmLdHRWXhKPNfqPc-06-01-2016_testcut_of_2533.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part907" data-index="265">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part909" data-index="266" style="margin-left:0;">2533-3/4/5 were sent in for sequencing </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part910" data-index="267" style="margin-left:0;">Prepped for creation of comp cells for tomorrow</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part911" data-index="268" style="margin-left:35px;">Mix 25g PEG8000 with 30ml water and disolve over night</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/2/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part912" data-index="269" style="margin-left:0;">PIck 2 colonies for each pGOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part913" data-index="270" style="margin-left:0;">Miniprepped dc16 and dc56</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part914" data-index="271" style="margin-left:0;">Prepared  200ml TSS Solution</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part915" data-index="272" style="margin-left:0;">dc 16 test cut with MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part916" data-index="273" style="margin-left:35px;">expected bands: 10723, 1172</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part917-item" data-formattable="true" data-index="274" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-02-2016 DC16 testcuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="AhIlY1fhkvZb7LGvV0f3o7kj80dlReRldAsa8DnD-06-02-2016_DC16_testcuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part917" data-index="274">DC16: 1,2,3,5 were good
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part919" data-index="275" style="margin-left:0;">dc 56 test cut with AgeIHF and EcoRIHF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part920" data-index="276" style="margin-left:35px;">expected bands: 6700, 1000</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part921" data-index="277" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part922" data-index="278" style="margin-left:0;">Primer 0170 for 2533 failed and this impeded the sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part923" data-index="279" style="margin-left:0;">Preparing Top10 comp cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part924" data-index="280" style="margin-left:35px;">First OD (optical density) measurement: 0.2 (time: 2:15 p.m.)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part925" data-index="281" style="margin-left:35px;">Second OD measurement: 0.34 (time 2:45 p.m.)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part926" data-index="282" style="margin-left:35px;">Third OD measurement: 0.419 (time 3:05 p.m.)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part927" data-index="283" style="margin-left:35px;">Fourth OD measurment: 0.486 (time 3:35 p.m.)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/3/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part928" data-index="284" style="margin-left:0;">miniprepped pGOP plasmids</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part929" data-index="285" style="margin-left:0;">test cut pGOP plasmids with Bbs1 and Spe1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part930-item" data-formattable="true" data-index="286" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-03-2016 pGOP testcuts 1-8.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="6mjyQMxVd4Ynr7gWkVn3P5VAuoMxQoNzHeYexVqO-06-03-2016_pGOP_testcuts_1-8.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part930" data-index="286">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part932-item" data-formattable="true" data-index="287" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-03-2016 pGOP9-20 testcuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="L9GS5uo2EU9ddMPkijaoSraeewfdM9H8F72NBIHS-06-03-2016_pGOP9-20_testcuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part932" data-index="287">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part934" data-index="288" style="margin-left:0;">grew up more _____ for midiprep</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 6/4/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part935" data-index="289" style="margin-left:0;">Picked three colonies of pGOP 20 (3, 4, and 5)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part936" data-index="290" style="margin-left:0;">Grew up pGOP 20-3/4/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part937" data-index="291" style="margin-left:0;">Miniprep 2533-7/8/9</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 6/5/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part938" data-index="292" style="margin-left:0;">Miniprep 20-3/4/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part939" data-index="293" style="margin-left:0;">Test Cut for 2533 and pGOP 20 addition colonies</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part940-item" data-formattable="true" data-index="294" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-05-2016 pGOP20 and 2533 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="raEniQFACMZ6WTWFCcHbcTjZDHj2n1sPDRniOu4f-06-05-2016_pGOP20_and_2533_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part940" data-index="294">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/6/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part942" data-index="295" style="margin-left:0;">Miniprep pGOP20-R1/R2/R3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part943" data-index="296" style="margin-left:0;">Test cuts for pGOP20-R1/R2/R3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part944" data-index="297" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part945" data-index="298" style="margin-left:0;">Cell Stock pGOP 1-19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part946" data-index="299" style="margin-left:0;">Grew up DC16-1 and DC56-1 for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part947" data-index="300" style="margin-left:0;">Sent pGOP 20-3/4/5 and 2533-7/8/9 for sequencing </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part948" data-index="301" style="margin-left:0;">Diluted pGEX 1-20 and pGOP 1-19 to 50 ng/uL</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part949" data-index="302" style="margin-left:0;">Made a liter of LB + Carb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part950" data-index="303" style="margin-left:0;">Nanodropped pGEX 1-20 and pGOP 1-19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part951" data-index="304" style="margin-left:35px;">Inoculated pGEX16-1 due to low concentration</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part952" data-index="305" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part953" data-index="306" style="margin-left:0;">Midiprep for Transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part954" data-index="307" style="margin-left:35px;">Prepared 150 mL of LB + Carb with pCJH2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part955" data-index="308" style="margin-left:35px;">Prepared 150 mL of LB + Carb with T40</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part956" data-index="309" style="margin-left:35px;">Prepared 150 mL of LB + Carb with pBW363</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/7/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part957" data-index="310" style="margin-left:0;">Plan for transfection of pGEXs and pGOPs to be performed on Thursday</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part958-item" data-formattable="true" data-index="311" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">new doc 7_1.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="RiqKnvcxp4977zdKMNwMbEj5tJeV85CDRWS2Ucyn-new_doc_7_1.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part958" data-index="311">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part960" data-index="312" style="margin-left:0;">Miniprep of pGOP12, pGEX16, DC174 and DC100 (both DCs will be used as positive controls transfection on Thursday)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part961" data-index="313" style="margin-left:0;">Midiprep of pBW363 (Blank), T40 (dCas9), pCJH2 (BFP transfection marker), DC16 and DC56</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part962" data-index="314" style="margin-left:35px;">Evaporated supernatant</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/8/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part963" data-index="315" style="margin-left:0;">Cell stocked pGOP 20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part964" data-index="316" style="margin-left:0;">50ul Restriction digest of 2490 and DC16 with MluI and NruI to create 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part965" data-index="317" style="margin-left:35px;">5ul buffer3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part966" data-index="318" style="margin-left:35px;">6ul DNA</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part967" data-index="319" style="margin-left:35px;">1.5ul Nru1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part968" data-index="320" style="margin-left:35px;">1.5ul Mlu1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part969" data-index="321" style="margin-left:35px;">36ul water</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part970" data-index="322" style="margin-left:35px;">incubate 1 hr in 37C</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part971" data-index="323" style="margin-left:35px;">Got 3 bands so threw out digest and are now investigating our enzymes to see if there has been contamination</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part972" data-index="324" style="margin-left:0;">Stocked midiprep DNA with 1x TE</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part973" data-index="325" style="margin-left:0;">Grew up T40 for further minipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part974" data-index="326" style="margin-left:0;">Prepared HEK293 plates for tomorrow's transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part975" data-index="327" style="margin-left:35px;">Add 250ul of 200000cells/ml cell solution to each well on 48 well plate</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/9/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part976" data-index="328" style="margin-left:0;">Transfected HEK cells</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part977-item" data-formattable="true" data-index="329" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.18.39.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JdPPlxzJdcUw397LN76mBuwsX1rODIWWWBP84d2D-Screen_Shot_2016-08-01_at_12.18.39.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part977" data-index="329">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part979" data-index="330" style="margin-left:0;">Miniprepped T40</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part980" data-index="331" style="margin-left:0;">Diluted pCJH2, pBW363, and T40 for transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part981" data-index="332" style="margin-left:0;">Checked and rediluted pGEXs and pGOPs for transfection </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part982" data-index="333" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part983" data-index="334" style="margin-left:0;">note-  pGEX11 is contaminated</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part984" data-index="335" style="margin-left:35px;">pGEX7 is too low of concentration</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/10/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part985" data-index="336" style="margin-left:0;">Checked transfection plates under a microscope</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part986" data-index="337" style="margin-left:35px;">all worked; however, Cre showed leakiness</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part987" data-index="338" style="margin-left:0;">Resent DC16-1 for sequencing due to QuintaraBio sequencing faillure</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part988" data-index="339" style="margin-left:0;">Passaging HEK293 cells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part989" data-index="340" style="margin-left:0;">Minipreppred pGOP1-20 (inoculated in Cam last night)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part990" data-index="341" style="margin-left:0;">Cut DC16 and 2490 with MluI and NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part991" data-index="342" style="margin-left:35px;">8 uL DC16  or 11uL of 2490</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part992" data-index="343" style="margin-left:35px;">1.5 uL MluI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part993" data-index="344" style="margin-left:35px;">1.5 uL NruI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part994" data-index="345" style="margin-left:35px;">5uL buffer 3.1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part995" data-index="346" style="margin-left:35px;">34 uL water or 31 uL water (2490)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part996" data-index="347" style="margin-left:0;">Run Gel Extraction on 2490 and DC16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part997" data-index="348" style="margin-left:35px;">Bands</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part998" data-index="349" style="margin-left:35px;">DC16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part999" data-index="350" style="margin-left:70px;">10723</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1000" data-index="351" style="margin-left:70px;">1172 - WANTED BAND</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1001" data-index="352" style="margin-left:35px;">2490</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1002" data-index="353" style="margin-left:70px;">10590 - WANTED BAND</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1003" data-index="354" style="margin-left:70px;">227</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1004-item" data-formattable="true" data-index="355" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-13-2016 dc16 2490.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tDdhcbr26BboQeWYaen04z07LQTpQYcO9pRcHrLE-06-13-2016_dc16_2490.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1004" data-index="355">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1006" data-index="356" style="margin-left:0;">Ligation of 2490 and DC16 to make 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1007" data-index="357" style="margin-left:0;">Ligations were transformed and plated</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1008" data-index="358" style="margin-left:35px;">Diluted primers for PCR reaction of pGPX1 (2531)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1009" data-index="359" style="margin-left:35px;">PCR GFP, BFP and mRuby </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1010" data-index="360" style="margin-left:35px;">Digested pGOPs 3,5,6,8,10,14,15,19 and pGPX1 with Mlu1-HF and Age1-HF in preparation of making pGOPs with GFP, BFP and mRuby</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1011-item" data-formattable="true" data-index="361" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-14-2016 pGOPs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="YnlkemaP7ktXgp5wOg6pUUHEjISRsU7uD4BHAeAL-06-14-2016_pGOPs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1011" data-index="361">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1013" data-index="362" style="margin-left:35px;">Miniprep 2531 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1014" data-index="363" style="margin-left:35px;">Picked colonies for 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1015" data-index="364" style="margin-left:35px;">Transfected HEK cells with different concentrations of the 8 pGOPs given above, to test whether the concentration of pGOPs in the cell was causing high levels of basal expression)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1016" data-index="365" style="margin-left:70px;">Transfected transfection marker, T40 (dCas9) pGEXs, Blank plasmid and pGOP.  pGOP at 62.5, 30 and 7.5 ng</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1017-item" data-formattable="true" data-index="366" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.17.24.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="xDW7aNbCjJtaDjqaCiMDH4UfL7xkmUKOW6WBWN3d-Screen_Shot_2016-08-01_at_12.17.24.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1017" data-index="366">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1019" data-index="367" style="margin-left:35px;">PCR digested GFP, BFP and mRuby with Mlu1-HF and Age1-HF</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1020-item" data-formattable="true" data-index="368" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-14-2016 Primers.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="9pc21ujJX4sFZ4ZCwdIVJ7aAAY4GW9fyQXTjuUW7-06-14-2016_Primers.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1020" data-index="368">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1022" data-index="369" style="margin-left:0;">Minipreppred T40 (was running low)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1023" data-index="370" style="margin-left:0;">Ligated GFP, BFP and mRuby into pGOP 3,5,6,8,10,14,15,19.  Transformed onto Cam plates</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1024" data-index="371" style="margin-left:0;">Ran FACs on transfection from Tuesday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1025" data-index="372" style="margin-left:0;">PCR the silencer out of DC100 (has UAS and pG5) to put into pGOPs and pGPX6 with PrA4 and PrA4r</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1026" data-index="373" style="margin-left:0;">Digested DC 100 and prepared annealed oligos o83-o122 to prepare _________</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 6/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1027" data-index="374" style="margin-left:0;">Miniprepped Transformed plasmids from 6-15-16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1028" data-index="375" style="margin-left:0;">PCR for GFP, BFP, and mRuby</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1029-item" data-formattable="true" data-index="376" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 GFP BFP mRuby.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="NBesFu9vyPTZJoK86NXj54vBTySURGy7BYIK1ceL-06-17-2016_GFP_BFP_mRuby.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1029" data-index="376">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1031-item" data-formattable="true" data-index="377" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 2534t and 2534b.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="eLwiuU57MQk4xygqtqf34Nyzxj2uwPIKClzUakSE-06-17-2016_2534t_and_2534b.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1031" data-index="377">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1033" data-index="378" style="margin-left:0;">Sent plasmdis in for sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1034" data-index="379" style="margin-left:0;">Sequence for 2534B-4 is verified as functioning and will be moved forward with cloning</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1035-item" data-formattable="true" data-index="380" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 bfp gfp pgops.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="HvHftjVaS7ph0FZAsDnmu2QjcEc92n9IsEg9ozAN-06-17-2016_bfp_gfp_pgops.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1035" data-index="380">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1037-item" data-formattable="true" data-index="381" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-17-2016 mRuby pGOPs and 2531.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="oRzmTDYjiOUva7UjQp3f6Yq7vdwa9cSCFHARGNDe-06-17-2016_mRuby_pGOPs_and_2531.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1037" data-index="381">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1039" data-index="382" style="margin-left:0;">Cell Stocked plasmids for BFP, GFP, and mRuby that sequenced properly</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1040" data-index="383" style="margin-left:0;">Miniprepped plasmids</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1041" data-index="384" style="margin-left:0;">Picked colonies for mRuby pGOP 10,14, and 15</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1042" data-index="385" style="margin-left:0;">Cell stocked BFP pGOP 15</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1043" data-index="386" style="margin-left:0;">Picked colonies for GFP pGOP 8, GFP pGOP 19, mRuby pGOP 10, mRuby pGOP 15, and mRuby pGOP 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1044" data-index="387" style="margin-left:0;">Miniprepped plasminds from 6-20-16 (mRuby pGOP 10-3, 10-4, 14-3, 14-5, 14-6, 15-3)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1045" data-index="388" style="margin-left:0;">Sent mRuby pGOP 14-3/4/5 and BFP pGOP 19-2 for sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1046" data-index="389" style="margin-left:0;">Passaged new HEK cells to P7</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1047" data-index="390" style="margin-left:0;">Made 2 new bottles of D5 Media (D5= 5%FBS)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1048" data-index="391" style="margin-left:35px;">500ml of DMEM base</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1049" data-index="392" style="margin-left:35px;">25ml FBS (alloquoted in fridge across hallway from TC room)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1050" data-index="393" style="margin-left:35px;">5ml L-Glut</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1051" data-index="394" style="margin-left:35px;">5ml P/S</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1052" data-index="395" style="margin-left:35px;">5ml sodium pyruvate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1053" data-index="396" style="margin-left:35px;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1054" data-index="397" style="margin-left:0;">Cell stocked picked colonies from 6/21/16 (<span style="color:#344043"><span style="background-color:#FFFFFF">GFP pGOP 8, GFP pGOP 19, mRuby pGOP 10, mRuby pGOP 15, and mRuby pGOP 19</span></span>).</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1055" data-index="398" style="margin-left:0;">Gel extract PrA4/4r (silencer) </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1056-item" data-formattable="true" data-index="399" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-23-2016 PCR of silencer.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="G8BxvwPcEI2hVwW8qzJh97BJhe9BUSCoFT3krwyb-06-23-2016_PCR_of_silencer.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1056" data-index="399">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1058" data-index="400" style="margin-left:0;">Digested DC100 with NheI and dropped in annealed oligos with ligation to make pDOP 1-20 (backbone from DC100 with gRNAs dropped in)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1059" data-index="401" style="margin-left:0;">sent pGOP 6-9 back in for sequencing - suspected error in gRNA target site</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 6/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1060" data-index="402" style="margin-left:0;">ligate and transform pDOP 1-20 (DC100 and annealed oligos)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1061" data-index="403" style="margin-left:0;">inoculate pGPX1 so we can make pGPX2 and 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 6/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1062" data-index="404" style="margin-left:0;">Prepped for transfextion</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1063" data-index="405" style="margin-left:0;">Picked pDOP1-20 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1064" data-index="406" style="margin-left:0;">Gel extracted  pGPX1</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 6/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1065" data-index="407" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">ligate and transfrom pGPX2 and pGPX3 (pGPX1  MluI Age1 with PrA1/1r and PrA2/2r Mlu1 and Age1)</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1066" data-index="408" style="margin-left:0;">miniprep pDOP 1-20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1067" data-index="409" style="margin-left:0;">Test Cut pDOP1-20 with NheI-HF and StuI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1068-item" data-formattable="true" data-index="410" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-29-2016 pDOP 1-10.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="oTpT0Hc2exGh4zcE7xKQDZiOmLlyvz4ojyI5rZOR-06-29-2016_pDOP_1-10.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1068" data-index="410">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1070-item" data-formattable="true" data-index="411" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-29-2016 pDOP 11-20.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="3z9wz2wOTF7PeCQGtK5K0iDgNZloPD3lrxNaMwoL-06-29-2016_pDOP_11-20.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1070" data-index="411">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1072" data-index="412" style="margin-left:0;">Transfected pGOP 3,5,6,8,10,14,15,19 of iRFP, BFP, GFP, and mRuby </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1073-item" data-formattable="true" data-index="413" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.16.09.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ffaCORLZdCeyl1DW9PulPv97fd4H4uHkRTf5pwan-Screen_Shot_2016-08-01_at_12.16.09.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1073" data-index="413">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 6/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1075" data-index="414" style="margin-left:0;">PIcked pGPX2 and pGPX3 colonies </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1076" data-index="415" style="margin-left:0;">Reannealed-phosphorylated- ligated - and transformed pDOPs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1077" data-index="416" style="margin-left:0;">recut pDOPs</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1078-item" data-formattable="true" data-index="417" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">06-30-2016 recut pDOPs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Qj9QP4QxBwOd5MMx56qn71vzQpgPDWIn2rL1aUpz-06-30-2016_recut_pDOPs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1078" data-index="417">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1080" data-index="418" style="margin-left:0;">Miniprep of GPX 2-1/2/3 and GPX 3-1/2/3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1081" data-index="419" style="margin-left:0;">Test-cut GPX 2 and 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1082" data-index="420" style="margin-left:0;">Picked colonies and grew up pDOP 1-4; 6-11; 14-20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1083" data-index="421" style="margin-left:0;">Ran FACS (flow cytometry)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1084" data-index="422" style="margin-left:35px;">Did not test BFP due to wrong transfection marker</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1085" data-index="423" style="margin-left:0;">PCR clean up of 2485; 2487; and 2489 with NotI and Cip</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1086" data-index="424" style="margin-left:0;">Gel extraction of 2484, 2486, 2488, and 2534 digested with AscI and NotI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1087-item" data-formattable="true" data-index="425" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-01-2016 pGPX2 3.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vNghMX61p19d8X0d3jORtlxpChGR5S7WO8hB2CDB-07-01-2016_pGPX2_3.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1087" data-index="425">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1089" data-index="426" style="margin-left:0;">Miniprep and test cut pDOP 1-4; 6-11; 14-20 colonies 1 and 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1090" data-index="427" style="margin-left:35px;">digested with NheI and PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1091" data-index="428" style="margin-left:0;">Vectors pGPX2 and pGPX3 are sequenced verifeid. pGOP 66,34, and 26 could not be sequenced properly (quintara error)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1092" data-index="429" style="margin-left:0;">Reenoculate pGEX and GFP pGOP 3, 5, 6, 8, 10, 14, 15, 19      gh54</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1093" data-index="430" style="margin-left:0;">Miniprep pGEX and GFP pGOP 3, 5, 6, 8, 10, 14, 15, 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1094" data-index="431" style="margin-left:0;">Gibson and transform pREC 1, 2, 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/4</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1095" data-index="432" style="margin-left:0;">Passage HEK cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1096" data-index="433" style="margin-left:0;">Inoculate pREC 1, 2, 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1097" data-index="434" style="margin-left:0;">Miniprep pREC 1, 2, 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1098" data-index="435" style="margin-left:0;">Enoculate BW1720</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1099" data-index="436" style="margin-left:0;">Digested BW1720, and BW1942-43 with BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1100" data-index="437" style="margin-left:0;">Digested BW758, and  BW762-65 with AgeI and EcoRI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1101" data-index="438" style="margin-left:0;">Digested DC 100 with NheI and Cip</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1102" data-index="439" style="margin-left:0;">Digested pGPX1-4 with Xbha and Bbsi</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1103-item" data-formattable="true" data-index="440" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-05-2016 dc100, pGPX1:2:3:4.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="AYg1ftPlRSrVCzZohTRniAOeHZ7E3LRMATOVOJpj-07-05-2016_dc100_pGPX1234.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1103" data-index="440">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1105-item" data-formattable="true" data-index="441" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-05-2016 BW contstructs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="EcsdVNTZozlZ5Uel33oVRR4mfEAiumKlmI9LIyHu-07-05-2016_BW_contstructs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1105" data-index="441">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1107-item" data-formattable="true" data-index="442" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-05-2016 pREC.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="HeoC8UONPeRA646xLXzYEBjt4yxV6BsneIjefjJZ-07-05-2016_pREC.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1107" data-index="442">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1109" data-index="443" style="margin-left:0;">Transfected HEK cells for gRNA orthogonality experiment</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1110-item" data-formattable="true" data-index="444" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.15.02.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="smQSiF4pGbCfYb9gknZvgPQFIxnMkFlUmpMgzzpL-Screen_Shot_2016-08-01_at_12.15.02.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1110" data-index="444">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1112" data-index="445" style="margin-left:0;">Miniprep pGEX, GFP pGOP, and mRuby pGOP 6&amp; 14</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1113" data-index="446" style="margin-left:0;">Digested 1720 with AgeI and EcoRI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1114-item" data-formattable="true" data-index="447" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-06-2016 1720.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="k18rRvOubLEXGhDvdawlLwvlnDtq0piBXcFT9k87-07-06-2016_1720.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1114" data-index="447">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1116" data-index="448" style="margin-left:0;">Redigested 2485, 2487, and 2489 with NotI and Cip</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1117-item" data-formattable="true" data-index="449" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-06-2016 2485 2487 2489.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ahXE7E33j1cHLOnrIJywpGtvWqxBv9k8vIW0MiCN-07-06-2016_2485_2487_2489.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1117" data-index="449">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1119" data-index="450" style="margin-left:0;">Ligated and transformed pBEX1-24 pPV1,5-8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1120" data-index="451" style="margin-left:0;">Retransformed pGEX6 and 14 pGOP26,34,66,74</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1121" data-index="452" style="margin-left:0;">Made LB + Carb plates</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1122" data-index="453" style="margin-left:0;">Picked colonies for pBEX 1 - 24, pGEX 6 and 14, GFP and mRuby pGOPs 6 and 14, adn pPVs 1, 5, 6, 7, 8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1123" data-index="454" style="margin-left:0;">Miniprep pGEX 1-4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1124" data-index="455" style="margin-left:0;">Ran FACs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1125" data-index="456" style="margin-left:0;">Grew more 2534</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1126-item" data-formattable="true" data-index="457" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_9323 copy.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="hJJ5fIChPvpyFYLYqEm0vuPgD6EUUy7Wmyuo58oC-IMG_9323_copy.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1126" data-index="457">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1128" data-index="458" style="margin-left:0;">Grew up pPV7 and pBEX 10, 12, 13, 17, 18, 22</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1129" data-index="459" style="margin-left:0;">Growing more 2534</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1130" data-index="460" style="margin-left:0;">Gibsoned pREC1, 2, and 3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1131" data-index="461" style="margin-left:0;">Passaged HEK cells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1132" data-index="462" style="margin-left:0;">Cell Stock pPV7 and pBEX 10, 12, 13, 17, 18, and 22</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1133" data-index="463" style="margin-left:0;">Digested pGPX2, mRuby2, and pGOP 1, 2, 4, 6, 7, 9, 11, 12 13, 16, 17, 18, and 20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1134" data-index="464" style="margin-left:35px;">mRuby2 and pGOPs digested with MluI and AgeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1135" data-index="465" style="margin-left:35px;">pGPX2 digested with BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1136" data-index="466" style="margin-left:35px;">Ran on a gel and gel extracted </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1137-item" data-formattable="true" data-index="467" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-12-2016 pGOP mRuby2 pGPX2.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tpR1ppA9J1tlaKS7r1vZDs6ip64C48QDXpDIYtUZ-07-12-2016_pGOP_mRuby2_pGPX2.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1137" data-index="467">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1139" data-index="468" style="margin-left:0;">Miniprep pGPX2, 1720, 1942-44</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1140" data-index="469" style="margin-left:0;">Digested pGPX2, 1720, 1942-44 with ________________</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1141" data-index="470" style="margin-left:0;">Re-digested pGPX2 (bbs1, extract 3kb band) ,mRuby2(mlu1, age1; extract 700bp band) ,and pGOP21 (mlu1,age1, extract 3kb band)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1142" data-index="471" style="margin-left:35px;">ran on gel and gel extracted</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1143-item" data-formattable="true" data-index="472" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-13-2016 pGPX2,mRuby,pGOP21.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="c1UXJTRKyVdyjr4VyNuHyV2FMAX4FpfHit9D3Thr-07-13-2016_pGPX2mRubypGOP21.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1143" data-index="472">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1145" data-index="473" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1146" data-index="474" style="margin-left:0;">Ligating and transforming </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1147" data-index="475" style="margin-left:35px;">pGOP 21 22 24 27 29 31 32 33 34 36 37 38 40 41 42 44 47 49 51 52 53 56 57 58 60 61 62 64 66 67 69 71 72 73 76 77 78 80</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1148" data-index="476" style="margin-left:0;">Digesting </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1149" data-index="477" style="margin-left:35px;">1270 758 763 765 with AgeI and EcoRI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1150-item" data-formattable="true" data-index="478" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 758 763 765 1720.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="AgRuyIo3HAhv87gPqnu0SOtg9rsP0plHncSYYIEA-07-14-2016_758_763_765_1720.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1150" data-index="478">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1152" data-index="479" style="margin-left:0;">Circuit Designs Digesting</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1153" data-index="480" style="margin-left:35px;">Part1 AscI NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1154" data-index="481" style="margin-left:35px;">Part2 <span style="color:#344043"><span style="background-color:#FFFFFF">AscI NotI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1155" data-index="482" style="margin-left:35px;">Part3 <span style="color:#344043"><span style="background-color:#FFFFFF">AscI NotI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1156" data-index="483" style="margin-left:35px;">Part4 <span style="color:#344043"><span style="background-color:#FFFFFF">AscI NheI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1157" data-index="484" style="margin-left:35px;">Dest <span style="color:#344043"><span style="background-color:#FFFFFF">EcoRI NotI</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1158" data-index="485" style="margin-left:0;">Circuits (P1-P2-P3-P4)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1159" data-index="486" style="margin-left:0;">pBEX1-pBEX6-pBEX15-pBEX24</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1160" data-index="487" style="margin-left:0;">pBEX1-pBEX10-pBEX19-pBEX20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1161" data-index="488" style="margin-left:0;">pBEX5-pBEx18-pBEX19-pBEX16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1162" data-index="489" style="margin-left:0;">pBEX5-pBEX2-pBEX15-pBEX8</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1163-item" data-formattable="true" data-index="490" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 pBEX 8 16 20 24.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tMPp5VtpeLwTg6YY1m32xCeBDdynzrqJrstcO2Ie-07-14-2016_pBEX_8_16_20_24.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1163" data-index="490">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1165-item" data-formattable="true" data-index="491" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 pBEXs.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="CEfg9psNCtUnY7b3DJ1quiDzL8vPYyZNMKnfSw8y-07-14-2016_pBEXs.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1165" data-index="491">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1167" data-index="492" style="margin-left:0;">Test cut 2578, 2579, 2580 (formely pRECs)</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1168-item" data-formattable="true" data-index="493" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-14-2016 2578 2589 2580.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="PpX97l1ddkWyX0XopiDPOc0NSzwuG8jZ4TYIsyF6-07-14-2016_2578_2589_2580.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1168" data-index="493">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1170" data-index="494" style="margin-left:0;">Miniprep 2578, 2579, and 2580 3-7 &amp; testcut</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1171-item" data-formattable="true" data-index="495" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-15-2016 2578 2579 2580 test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="gJObOL4E1ioK87khT7qzU3Ot37dfKuyAASv4ucbN-07-15-2016_2578_2579_2580_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1171" data-index="495">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1173" data-index="496" style="margin-left:0;">2578-2 and 2579-1 were sequenced verified adn moved to cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1174" data-index="497" style="margin-left:0;">Minipreped 1716 pDest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1175" data-index="498" style="margin-left:0;">Digested pDest with EcoRI and NotI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1176-item" data-formattable="true" data-index="499" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07-15-2016 1716.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="MOIIA8OvBcxCPCCk9JccqWgrcHtMoYaIWN55yIOp-07-15-2016_1716.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1176" data-index="499">
-</div></div></div></div></div><div class
-="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1178" data-index="500" style="margin-left:0;">Religated pPV1, pPV6, and pPV8</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1179" data-index="501" style="margin-left:0;">Picked new pGOP colonies for GFP only</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1180" data-index="502" style="margin-left:0;">Miniprepped new GFP clonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1181" data-index="503" style="margin-left:0;">Minprepped 2580-8 through 2580-17</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1182" data-index="504" style="margin-left:0;">Transformed pCir1 pCir2 and pCir3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1183" data-index="505" style="margin-left:0;">Test Cuts for 2580 aditional colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1184" data-index="506" style="margin-left:0;">Test Cuts for pPV1, pPV6, and pPV8 additional colonies</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1185" data-index="507" style="margin-left:0;">Test Cuts for pGOP GFP additional colonies</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1186" data-index="508" style="margin-left:0;">Test Cuts for pCIR1, pCir2, and pCir3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1187" data-index="509" style="margin-left:0;">Test Cuts for pGOp1 and 2 under BFP and mRuby</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1188" data-index="510" style="margin-left:0;">Split HEK cells 1:10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1189" data-index="511" style="margin-left:0;">Created two 48 well  plates at 200,000 cells/ml for transfection of BFP pGOP constructs </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1190" data-index="512" style="margin-left:0;">Created two T175plates with 25ml of 100,000cells/ml for stable integration</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1191" data-index="513" style="margin-left:0;">Midiprep BW361, BW465, BW471, BW 474, 2578, and 2579</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1192" data-index="514" style="margin-left:0;">Digested pBEX 19 with AscI and NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1193" data-index="515" style="margin-left:35px;">Gel extracedracted  pBEX 19</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1194-item" data-formattable="true" data-index="516" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07192016 pBEX19.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="VF3g6RErnr18sRETBB2fk628MQB5A60yiIKhBmKc-07192016_pBEX19.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1194" data-index="516">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1196" data-index="517" style="margin-left:0;">Digested pCir 1-3, 1-1, 3-2, 3-3 with Not1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1197-item" data-formattable="true" data-index="518" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07192016 pcirtestcuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="2mYxgnPtjdl4LeKcIU5IXraiK65M0KUurg5ocznc-07192016_pcirtestcuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1197" data-index="518">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1199" data-index="519" style="margin-left:0;">Transfected BFP</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1200-item" data-formattable="true" data-index="520" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 00.01.59.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="s8BAZnYNjLQiiFMHdY3XY7Ysi9s6QN5WhsYk430T-Screen_Shot_2016-08-01_at_00.01.59.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1200" data-index="520">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1202-item" data-formattable="true" data-index="521" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_0128.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ci78q40lvuDsyThTfcxUy1UcOaIJ5R3rUk5qUnRA-IMG_0128.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1202" data-index="521">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1204" data-index="522" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1205" data-index="523" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1206" data-index="524" style="margin-left:0;">Performed FACs on the BFP transfected cells. Tried to create the data in MEFL but had trouble with getting the channel names to work with the software.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1207" data-index="525" style="margin-left:0;">Split HEK cells to P16. Expanded them into large T175 plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1208" data-index="526" style="margin-left:0;">Transfected the HEK cells in T175 with 2578 and 2579. Brought media volume up to 50ml to do so</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1209-item" data-formattable="true" data-index="527" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_1338.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="pXXqtxKOLiZO7PkSPbXESQXZpxRCuXZBklYdPAha-IMG_1338.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1209" data-index="527">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1211-item" data-formattable="true" data-index="528" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07222016 pCir test cut2.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="uysFA617h6fYOu2dAlEOhJMw3GFR6PEOZmY3Rxqz-07222016_pCir_test_cut2.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1211" data-index="528">will did a testcut of pCir1 and pCir3. sent one of each in for sequencing.
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1213" data-index="529" style="margin-left:0;">added puromyocin to the two stable line integrations of 2578 and 2579. 2ug per ml so we added 10ul of puromyocin to 50ml of media in the flask</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1214" data-index="530" style="margin-left:35px;">many more cells ttoday than yesterday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1215" data-index="531" style="margin-left:0;">Sent in a sample of the pGOP41 used in the previous transfection to troubleshoot what went wrong and why that particular gRNA was not working with BFP</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1216-item" data-formattable="true" data-index="532" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07232016 BFP PCR.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="mw7RHlZVpdgg12fGyVVs6E554HOZgFRSvR5uhqFg-07232016_BFP_PCR.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1216" data-index="532">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/24</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1218" data-index="533" style="margin-left:0;">Split the stable line integration HEK cells of 2578 and 2579 each into two new T175 flasks because they were too crowded. Added puromyocin to the fresh media.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1219" data-index="534" style="margin-left:0;">Transformed (GFP) pGOP 26, 27, 32, 33, 38, 40</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1220" data-index="535" style="margin-left:35px;">(BFP): pGOP 41, 42, 43, 44, 46, 47, 49, 51, 52, 53, 56, 57, 58, 60</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1221" data-index="536" style="margin-left:35px;">(mRuby): pGOP 61, 62, 64, ,66, 67, 69, 71, 72 73, 76, 77, 78, 80</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 7/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1222" data-index="537" style="margin-left:35px;">PIcked colonies from all transformed GFP pGOPs and less successful BFPs and mRubys</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1223" data-index="538" style="margin-left:35px;">Prepped overnight ligation for the BFPs and mRubys</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1224" data-index="539" style="margin-left:70px;">due to miscommunication, the ligation mix was made incorrectly but we went ahead anyway</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1225" data-index="540" style="margin-left:35px;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1226-item" data-formattable="true" data-index="541" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07252016 pGOP cuts and BFP mRUBY.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="RtdkVleUNsEWpu7rS0sCdUJudmeYNPr5ccpZfleL-07252016_pGOP_cuts_and_BFP_mRUBY.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1226" data-index="541">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 7/26</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1228" data-index="542" style="margin-left:0;">Digested pBEX 1,2,5,6,16,19 (AscI and NotI except 16 which was AscI and NheI) and 1716 (EcoRI and NotI) and pGOP68 and 35 (MluI and AgeI)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1229" data-index="543" style="margin-left:35px;">did not extract the pGOPs or 1716</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1230" data-index="544" style="margin-left:35px;">extracted the rest</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1231" data-index="545" style="margin-left:35px;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1232-item" data-formattable="true" data-index="546" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07262016 pBEX cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="L7yNw3j3sjnY8spe2uL5pU6ANt3KhSZVW3BuiR0w-07262016_pBEX_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1232" data-index="546">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1234" data-index="547" style="margin-left:0;">Inoculated pBEX 1,6,16,19, and 1716 and pGPX2 (so we could drop in the new oligos)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1235" data-index="548" style="margin-left:0;">Transformed DNA from overnight ligation </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1236" data-index="549" style="margin-left:0;">made Cam plates</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1237-item" data-formattable="true" data-index="550" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07262016 pgop.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="nEJFDNTscb6ROG7ROFWIiHy1IxLZob5fm08egeqN-07262016_pgop.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1237" data-index="550">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 7/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1239" data-index="551" style="margin-left:0;">Transformation did not work</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1240" data-index="552" style="margin-left:0;">Digested 1716 (EcoRI and NotI) and pGOP68 and pGOP35 (MluI and AgeI)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1241" data-index="553" style="margin-left:35px;">gel extracted</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1242-item" data-formattable="true" data-index="554" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07272016 pGOP35, 68 1716.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="lkneClrCTjyfxrNSSVGfxRWGNp9ZWEpzzHyW95yV-07272016_pGOP35_68_1716.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1242" data-index="554">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1244" data-index="555" style="margin-left:0;">annealed multimerized oligos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1245" data-index="556" style="margin-left:0;">digested more pGPX</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1246-item" data-formattable="true" data-index="557" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07272016 pGOP and pGPX digest.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="nH90ToeepVdGj9sKTM32svri3O2ahzppjXWNnN7f-07272016_pGOP_and_pGPX_digest.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1246" data-index="557">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1248" data-index="558" style="margin-left:0;">Diluted all the DNA for the transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1249" data-index="559" style="margin-left:0;">Transfected HEK cells with the "mini circuit" (1902/1903, pGOP1, pGOP22, BW390 (constituitive cre) and 2196 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1250" data-index="560" style="margin-left:35px;">also transfected pGOP22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 to test all of the GFPs. we did not have pGOP 21 and we did not have pGOP 40. We did not have pGEX11 to transfect so pGOP31 should not work. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1251" data-index="561" style="margin-left:0;">organized our DNA boxes</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1252-item" data-formattable="true" data-index="562" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-08-01 at 12.13.53.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="tdruuEqVouvF8zQROLwEpvxUFVtLxBIPC2BA5722-Screen_Shot_2016-08-01_at_12.13.53.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1252" data-index="562">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 7/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1254" data-index="563" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 7/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1255" data-index="564" style="margin-left:0;">Digested more BFP and mRUBY</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1256" data-index="565" style="margin-left:35px;">26ul pGOP55				30ulpGOP64</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1257" data-index="566" style="margin-left:35px;">1.5 of MLU1-HF &amp;Age1-HF		1.5 of Mlu1-HF &amp;Age1-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1258" data-index="567" style="margin-left:35px;">5 cutsmart					5 cutsmart</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1259" data-index="568" style="margin-left:35px;">20 h2o						16 h2o</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1260-item" data-formattable="true" data-index="569" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07292016 mRuby BFP.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="15vxSSYYlKurHynUbhYvPjfImfTmgSlOxEI2O83k-07292016_mRuby_BFP.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1260" data-index="569">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1262" data-index="570" style="margin-left:0;">ligated to create pGOP41, 51, 82, 83 to se what wasnt working with our cloning by testing our T4 ligase vs Wong labs t4 ligase</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1263" data-index="571" style="margin-left:0;">Testcut pCir1-6, -7, -8, -9, -10, -11, -12, -13, -14, -15, pCir3-4 with Acs1 Not1-HF</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1264-item" data-formattable="true" data-index="572" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07292016 pCir test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="wBPNZHZKB0avYwAF0xnlfFOOnw7f9Lo2LKeR7i7I-07292016_pCir_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1264" data-index="572">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1266" data-index="573" style="margin-left:0;">Cell stocked pGOP38-4, pGOP42-1, pGOp43-1, pGOP44-1, pGOP26-5, pGOP27-4, pGOP32-4, pGOP32-4, pGOP33-4, pGOP60-1, pGOP64-1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1267" data-index="574" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1268" data-index="575" style="margin-left:0;">performed FACs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1269" data-index="576" style="margin-left:0;">Split Deboki's cells for her and took a t175 plate at 1:10. (passage again on monday)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1270" data-index="577" style="margin-left:0;">Moved the stable line integrations to T50 plates. added 2ul of puromycin to 10ml of media</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1271" data-index="578" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1272" data-index="579" style="margin-left:0;">Picked colonies for GOP 82, and 41, 51 (no colonies for 83), pCIR1s that Ben made (picked 10 colonies)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1273" data-index="580" style="margin-left:0;">Met with Leidy to discuss what we are doing wrong with our ligation and transformation protocol. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1274" data-index="581" style="margin-left:0;">Re did digesestion of BFP and mRuby from pGOP 43 and 64, respectively. (age1 mlu1)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1275" data-index="582" style="margin-left:0;">Redigested pgpx2 to drop in the oligos.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1276" data-index="583" style="margin-left:0;">Religating fluorescence into regular pGOPs</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 7/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1277" data-index="584" style="margin-left:0;">Picked colonies of pGOP 66-1*(failed),-2, 67-1*(failed),-2, 71-1*(failed),-2, 73-1*(failed),-2, 76-1*(worked),-2, 46-1*(worked),-2, 47-1*(failed),-2., 51-1*(failed),-2, 52-1*(failed), 53-1*(worked),-2, 56-1(failed)*,-2, 49-1*(failed),-2, 69-1*(failed sequencing reaction),-2, 41-1*(worked),-2,-3 -4,-5, 51-1,-2,-3,-4,-5,-6, 40-1,-5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1278" data-index="585" style="margin-left:0;">pGOP 81-1,-2, 82-1*,-2, 83-1,-2,84-1,-2, 85-1,-2, 82-0* (-0 means grew up day before), 86-1,-2,87-1,-2,88-1*,-2, 89-1,-2, 90-1,-2, 83-0</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1279" data-index="586" style="margin-left:0;">pCIR1 -16,-17-,-18, -19,-20*,-21,-22,-23, -24,-25*,-26*,-27*, Ben's1, Ben's2*</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1280" data-index="587" style="margin-left:35px;">(starred**** ones sent for sequencing)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 7/31</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1281" data-index="588" style="margin-left:0;">miniprep of all of the colonies picked yesterday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1282" data-index="589" style="margin-left:0;">Ran test cuts of all of the colonie picked</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1283-item" data-formattable="true" data-index="590" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pCIR test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="lmLQvS2CgGQec3efgEz7AkI6BRwb5iolJf7vo9Nk-07312016_pCIR_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1283" data-index="590">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1285-item" data-formattable="true" data-index="591" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pGOP80 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="EXHnp5yYGcu8OaP4vaG5e17c02rHxWuWIbKdeTWF-07312016_pGOP80_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1285" data-index="591">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1287-item" data-formattable="true" data-index="592" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pGOP test cuts more.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="86Dw4nWHylAkYMygxEDNMnaYLHdopKA8K52TLPmd-07312016_pGOP_test_cuts_more.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1287" data-index="592">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1289-item" data-formattable="true" data-index="593" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">07312016 pGOP cuts again.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="J4n8TC5hpL1JLyx91BIIZCf7Zsr3WLV9XXQc8D0D-07312016_pGOP_cuts_again.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1289" data-index="593">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1291" data-index="594" style="margin-left:0;">split HEK cells 1:10 from large flask into small flask.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1292" data-index="595" style="margin-left:0;">let stable line of cells grow for another day.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1293" data-index="596" style="margin-left:0;">Grew up....  pGEX11, pGEX12, pGEX13, pGEX17, pGEX18, pGEX20, pGOP6(x2),pGOP21, pGOP22, pGOP23, pGOP24, pGOP25, pGOP26, pGOP27, pGOP28, pGOP29, pGOP30, pGOP31, pGOP32, pGOP33, pGOP34, pGOP35, pGOP36, pGOP37, pGOP38, pGOP39, pGOP42, pGOP43, pGOP44(x2), pGOP45(x2), pGOP50, pGOP54, pGOP55(x2), pGOP59, pGOP68, pGPX2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1294" data-index="597" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1295" data-index="598" style="margin-left:0;">(Will and Marisa) miniprepped pGEX11, pGEX12, pGEX13, pGEX17, pGEX18, pGEX20, pGOP6(x2),pGOP21, pGOP22, pGOP23, pGOP24, pGOP25, pGOP26, pGOP27, 		pGOP28, pGOP29, pGOP30, pGOP31, pGOP32, pGOP33, pGOP34, pGOP35, pGOP36, pGOP37, pGOP38, pGOP39, pGOP42, pGOP43, pGOP44(x2), pGOP45(x2), pGOP50, 	pGOP54, pGOP55(x2), pGOP59, pGOP68, pGPX2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1296" data-index="599" style="margin-left:0;">Grew up pGOP41, pGOP46, pGOP53, pGOP71, pGOP76 for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1297" data-index="600" style="margin-left:0;">Split the stable line HEK cells into new T75 flasks (p18)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1298" data-index="601" style="margin-left:0;">suspended the crRNA gBlocks oligos in 1xTE</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1299" data-index="602" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1300" data-index="603" style="margin-left:0;">Digested pGPX2 with BBs1</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1301-item" data-formattable="true" data-index="604" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160803 jeffery's stuff.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="aH19yZLA39RIzgKGv8HYVq9SKipBdFIP3NvTDouu-20160803_jefferys_stuff.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1301" data-index="604">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1303" data-index="605" style="margin-left:0;">DIgested pGOP76-1 and pGOP 41-1 for mRuby and BFP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1304" data-index="606" style="margin-left:0;">Transformed pGOPs 57, 58, 61, 62, 66, 77, 78, 81, 84, 85, 86, 87, 88, 89 using Leidys transformation protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1305" data-index="607" style="margin-left:35px;">use Ben's Top Ten cells, let cells sit on ice</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1306" data-index="608" style="margin-left:35px;">add 10ul of 5x KCM to ligation mix</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1307" data-index="609" style="margin-left:35px;">add 30ul of DI H2O to ligation mix</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1308" data-index="610" style="margin-left:35px;">add all 50 ul to cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1309" data-index="611" style="margin-left:35px;">plated 95ul of cells onto 1/2 plate</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1310" data-index="612" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1311" data-index="613" style="margin-left:0;">Sent sequencing pGOP 47-1(worked), 49-1(worked), 51-2(worked), 52-1(worked), 56-2 (failed), 67-1(worked), 69-1(worked), 71-1(FAIL), 72-1(worked), 73-1(resendfor seuquencing), 83-0(resend for sequencnig)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1312" data-index="614" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1313" data-index="615" style="margin-left:0;">Cell stocked pGOP41, pGOP46, pGOP53(need to throw awayb/c miscommunication), pGOP76 (throw away b/c miscommunication)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/4</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1314" data-index="616" style="margin-left:0;">(Marisa) Picked 2 colonies for pGOPs 57, 58, 61, 62, 66, 77, 78, 81, 84, 85, 86, 87, 88, 89</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1315" data-index="617" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1316" data-index="618" style="margin-left:0;">(Kami) Split wildtype cells 1:20 to P19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1317" data-index="619" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1318" data-index="620" style="margin-left:0;">(Jeffery) dimerized guides 1, 3, 8, 13 in various configurations with each other (1-3, 3-1, 8-13, 13-8)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1319" data-index="621" style="margin-left:35px;">this involved PCR and a gel extraction</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1320" data-index="622" style="margin-left:35px;">Designed decoders </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1321-item" data-formattable="true" data-index="623" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 dec2.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="yPemrWs1yagTZ6vMpTl7yGUPMNKryQNboZ5U7ame-20160805_dec2.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1321" data-index="623">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1323-item" data-formattable="true" data-index="624" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 decoder parts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="uvUQsVbJ5DrTUjgsOEnbT8zwd90VrNYENZ8nmn6y-20160805_decoder_parts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1323" data-index="624">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1325" data-index="625" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1326" data-index="626" style="margin-left:0;">(Will) Grew up pGOP 47, 49, 51, and 82-0 for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1327" data-index="627" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1328" data-index="628" style="margin-left:0;">Due to a miscommunication error, we had to retransform and plate pGOP52-1, pGOP53-1, pGOP67-1, pGOP69-1, pGOP71-2, pGOP72-1, pGOP73-1. We did this with our typical transformation protocol.</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1329" data-index="629" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1330" data-index="630" style="margin-left:0;">(Marisa and Will) Miniprepeed pGOPs 57, 58, 61, 62, 66, 77, 78, 81, 85, 86, 87, 88, and 89</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1331" data-index="631" style="margin-left:35px;">Due to a miscommunication error, miniprepped pGOP 47, 49, 51, and 82-0</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1332" data-index="632" style="margin-left:0;">(Will) Gibsoned </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1333" data-index="633" style="margin-left:35px;">pCir 2 using 1716, A, B, C, D</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1334" data-index="634" style="margin-left:35px;">pCir 2 using 1716 Part1/2, Part 3/4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1335" data-index="635" style="margin-left:35px;">pCir 2 usng 1716, Decoder 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1336" data-index="636" style="margin-left:35px;">pCir 4 using 1716, lambda, Part 3/4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1337" data-index="637" style="margin-left:35px;">pCir 1 using 1716, alpha, Bex19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1338" data-index="638" style="margin-left:35px;">pCir 1 using 1716, Part 1/2, Bex19, Bex16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1339" data-index="639" style="margin-left:35px;">pCir 1 using 11716 Bex 1, Bex6, Bex19, Bex16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1340" data-index="640" style="margin-left:35px;">pCir 3 using 1716, Bex5, Bex2, Bex19, Bex16</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1341" data-index="641" style="margin-left:35px;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1342" data-index="642" style="margin-left:0;">(Kami) Split stabel line (2578 and 2579) 1:10 in T75</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1343" data-index="643" style="margin-left:0;">(Rachel) Test-cut pGOPs <span style="color:#344043"><span style="background-color:#FFFFFF">57, 58, 61, 62, 66, 77, 78, 81, 85, 86, 87, 88, and 89</span></span></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1344-item" data-formattable="true" data-index="644" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 pGOP57-78 test cuts.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="PJqzNS2X4CDpBCI6RczRnLaP1Ryj7F2fHwtowQLT-20160805_pGOP57-78_test_cuts.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1344" data-index="644">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1346-item" data-formattable="true" data-index="645" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">20160805 pGOP81-89 test cut.jpeg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="ScjbHlUMbmpZuezUUx2AhTxJCsBXMkEYJjW9XIVc-20160805_pGOP81-89_test_cut.jpeg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1346" data-index="645">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1348" data-index="646" style="margin-left:0;">(Rachel) digested pGPX2 (5mg) with 3ul of BbsI. Double digested - too low of concentraiton to proceed with ligation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1349" data-index="647" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 8/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1350" data-index="648" style="margin-left:0;">(Will) grew up </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1351" data-index="649" style="margin-left:0;">(Jeffery) <span style="color:#000000">Digested pgpx2 with bbsi</span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1352" data-index="650" style="margin-left:0;">(kami) made WT Hek plates with Yash and split WT and 2578 and 2579 integrated cells 1:10</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1353" data-index="651" style="margin-left:0;">(will) cell stocked pGPX2 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1354" data-index="652" style="margin-left:0;">(Will) Test cut the pCir gibsons with Asc1 and Not1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1355" data-index="653" style="margin-left:0;">(Will) transformed pGOP 84. 86, 87, 88, 89, 90 with a ligation using Leidy's protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1356" data-index="654" style="margin-left:35px;">transformed pgOP47, 49, 51, 82-0 without a ligation using Leidy's protocol</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1357" data-index="655" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/9</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1358" data-index="656" style="margin-left:0;">(Rachel) Growing pCir4_2_4 from stab plate as sequencing indicated it worked!</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1359" data-index="657" style="margin-left:0;">(Rachel) Picked colonies from pGOP 81,84,86,87,88,89,90</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1360" data-index="658" style="margin-left:0;">(Rachel) made more LB + Cam broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1361" data-index="659" style="margin-left:0;">(Kami) autoclaved tips and agar</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1362" data-index="660" style="margin-left:0;">(Marisa and Kami) made carb plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1363" data-index="661" style="margin-left:0;">(Jeffrey) performed overhang extension PCRs on G-blocks (that make decoder) to build Circuit 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1364" data-index="662" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/10</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1365" data-index="663" style="margin-left:0;">(Marisa and Will) miniprep pGOP 81,84,86,87,88,89,90</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1366" data-index="664" style="margin-left:35px;">test cut with speI and BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1367" data-index="665" style="margin-left:0;">(Marisa) Cell Stock pGOP 47, 49, 51, and 82; pCir 4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1368" data-index="666" style="margin-left:35px;">miniprep pCir 4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1369" data-index="667" style="margin-left:0;">(Jeffrey) Digested Bex 19 and 16</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1370" data-index="668" style="margin-left:0;">(Kami and Marisa) grew up BW 363, 390, 391; pSB1C3; and T40 for midiprep</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1371" data-index="669" style="margin-left:0;">(Kami) split WT, 2578, 2579 HEK cells into T175</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1372" data-index="670" style="margin-left:0;">(Jeffrey) Picked Colonies for 87 and 88</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1373" data-index="671" style="margin-left:0;">(Marisa) Midiprep BW 363, 390, 391, T40, pSB1C3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1374" data-index="672" style="margin-left:0;">(Jeffrey) MIinprepped and test 87 and 88... picked colonies for 90. Transformed interlab study plasmids</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1375" data-index="673" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Marisa) Midiprep BW 363, 390, 391, T40, pSB1C3</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1376" data-index="674" style="margin-left:35px;">Cell stock pGOP 87</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1377" data-index="675" style="margin-left:0;">(Marisa and Rachel) Gel extracted </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1378" data-index="676" style="margin-left:0;">(Jeffrey) Mini prep and test cut 90... Retransformed interlab device 1</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 8/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1379" data-index="677" style="margin-left:0;">(Marisa and Rachel) Cell stock Interlab study: Pos 1, Neg 1, Part 2-1, Part 2-2, Pos 2, Neg 2, Part 3-1, Part 3-2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1380" data-index="678" style="margin-left:35px;">Miniprep Interlab Study and pGOP 56-3/4/5, pGOP 83-5/6/7/8/9</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1381" data-index="679" style="margin-left:0;">(Rachel) Digested pGOPs 56 and 83 with SpeI and XmaI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1382" data-index="680" style="margin-left:0;">(Marisa) Test Cut pGOPs 56 and 83</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1383" data-index="681" style="margin-left:0;">(Rachel) Ligate </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1384" data-index="682" style="margin-left:0;">(Jeffrey and Marisa) picked and grew up colonies: pGOP 57-1, 61-1/2/3, 62-1/2/3, 66-1/2/3, 77-1/2/3, 78-1/2/3, 80-1/2/3, 88-1/2/3/4/5, 90-1/2/3/4/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1385" data-index="683" style="margin-left:0;">(Kami and Marisa) Diluted and nanodropped </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1386" data-index="684" style="margin-left:0;">(Kami) made 6 plates for transfection. 3 with WT, 2 with stable line integrations of 2578 and 2579 each.</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1387" data-index="685" style="margin-left:0;">(Marisa) miniprepped <span style="color:#344043"><span style="background-color:#FFFFFF">pGOP 57-1, 61-1/2/3, 62-1/2/3, 66-1/2/3, 77-1, 78-1/2/3, 80-1/2/3, 88-1/2/3/4/5, 90-1/2/3/4/5</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1388" data-index="686" style="margin-left:0;">(Will) Digested Marisa's minipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1389" data-index="687" style="margin-left:0;">(Kami and Rachel) 7:30 pm transfected HEK cells to test the multimerization and the circuit</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1390" data-index="688" style="margin-left:35px;">for the circuit. Transfected 1/8 of 250ng of each component. (2 pgops made up 1/8 of the ng transfection and the extra was filled with Luis's pSB2C3 blank)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1391" data-index="689" style="margin-left:0;">(Kami And rachel) 2 hours after transfection, we added 4OHT/Absistic acid/Rapalog to appropriate wells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1392" data-index="690" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1393" data-index="691" style="margin-left:0;">(Kami and Rachel) prepared the transfected cells for FACS, and ran facs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1394" data-index="692" style="margin-left:0;">(Kami) Cells stocked stabel lines of HEK 2578(m150) and 2579 (m151)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1395" data-index="693" style="margin-left:35px;">trypsonize cel</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1396" data-index="694" style="margin-left:35px;">neutralize with media</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1397" data-index="695" style="margin-left:35px;">spin cells down</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1398" data-index="696" style="margin-left:35px;">remove media and break up pellet. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1399" data-index="697" style="margin-left:35px;">resuspend pellet in 90%FBS 10%DMSO (1ml per cryotube)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1400" data-index="698" style="margin-left:35px;">one T75 makess 2-3 cell stocks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1401" data-index="699" style="margin-left:0;">(Marisa) Made LB Broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1402" data-index="700" style="margin-left:35px;">inoculated T40 and BW363 in falcon tubes</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1403" data-index="701" style="margin-left:35px;">turn LB Broth into LB + Carb broth</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1404" data-index="702" style="margin-left:0;">(Kami) made cam agar and broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1405" data-index="703" style="margin-left:35px;">miniprep pGOP 2, 6, 83-11/12/13/14/15</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1406" data-index="704" style="margin-left:0;">(Marisa) made Cam plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1407" data-index="705" style="margin-left:35px;">Cell stocked pGOP 57, 61, 80, 88, and 90</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1408" data-index="706" style="margin-left:35px;">inoculated 1mL of each T40 and BW363 sampled into flasks with 150 mL LB + Carb</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1409" data-index="707" style="margin-left:35px;">Digested pGOPs 83 with SpeI and BbsI for test cut</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1410" data-index="708" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1411" data-index="709" style="margin-left:0;">(Marisa) Midiprep three of both T40 and BW363</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1412" data-index="710" style="margin-left:0;">(Marisa) Grew up pGOP 82</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1413" data-index="711" style="margin-left:0;">(Jeffrey) Grew up pGOP 83-14</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1414" data-index="712" style="margin-left:0;">(Jeffrey) took OD 600 of the interlab and diluted cells down to .02</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1415" data-index="713" style="margin-left:0;">(Will) PCR'ed the pCMV fro the iGEM registry</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1416" data-index="714" style="margin-left:0;">(Will) Gibsoned and transformed the CMV PCR product into pGEX</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1417" data-index="715" style="margin-left:0;">(Marisa) made 96 well plate for InterLab Study</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1418" data-index="716" style="margin-left:35px;">250 uL DPBS and 2uL cells per sample</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1419" data-index="717" style="margin-left:0;">(Kami Rachel) prepared HEK plates for transfection at 200,000 cells </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1420" data-index="718" style="margin-left:0;">(Marisa) Cell stock pGOP 83</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1421" data-index="719" style="margin-left:35px;">Grew up BW 1945, 1946, 1947, and 1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1422" data-index="720" style="margin-left:0;">(Will) Miniprep pGOP 82 ad 83</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1423" data-index="721" style="margin-left:0;">(Jeffrey) Picked colonies from GFP and BFP under igem CMV</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1424" data-index="722" style="margin-left:0;">(Jeffrey) Transformed and plated interlab bacteria </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1425" data-index="723" style="margin-left:0;">(Kami Rachel Marisa) made trasfection DNA mixes and transfected the HEK cells we plated yesterday</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1426" data-index="724" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1427-item" data-formattable="true" data-index="725" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_145414238.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vyJM6utqStHPDJT7MEzr8eY3bZeoeR49FL0vgqqu-IMG_20160823_145414238.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1427" data-index="725">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1429-item" data-formattable="true" data-index="726" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_145437000_HDR.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="4VedDMnb5xw6XvKMWr7qj2Dl129lUE7JQ2ROGsqS-IMG_20160823_145437000_HDR.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1429" data-index="726">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1431-item" data-formattable="true" data-index="727" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_150756390_HDR.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="lhWTUAThFdvzBtPLxL88jCw7IwPVUZXSr1vTdNae-IMG_20160823_150756390_HDR.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1431" data-index="727">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1433-item" data-formattable="true" data-index="728" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">IMG_20160823_151151878_HDR.jpg</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="vm8AcKbwsufChsrE7iBspYO8q3UUflLVBe2QMWBt-IMG_20160823_151151878_HDR.jpg"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1433" data-index="728">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1435" data-index="729" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1436-item" data-formattable="true" data-index="730" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.39.05.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="YVfhCtdgMu02dQlK1NY3dksXRMvpOwyt8oPHNBTi-Screen_Shot_2016-10-10_at_11.39.05.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1436" data-index="730">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1438" data-index="731" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1439-item" data-formattable="true" data-index="732" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.39.27.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JJO4oHKRdXhjb7H69jw2HneiAB5FbQi6OZOJId95-Screen_Shot_2016-10-10_at_11.39.27.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1439" data-index="732">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1441-item" data-formattable="true" data-index="733" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.39.37.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="uKtZCit3zbjRLztxqc58kSxSb6TqoKNUazpqyLmH-Screen_Shot_2016-10-10_at_11.39.37.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1441" data-index="733">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1443" data-index="734" style="margin-left:0;">Added drugs to inducible cells 2hrs after transfection</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1444" data-index="735" style="margin-left:35px;">Drugs are located in pink box labeled "Small molecules"</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1445" data-index="736" style="margin-left:70px;">Rapalog= AC heterodimerizer (1000x)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1446" data-index="737" style="margin-left:70px;">Absistic Acid (1000x)- vortex before use to get rid of percipitant</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1447" data-index="738" style="margin-left:70px;">4OHT (1000x)</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/24</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1448" data-index="739" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1449" data-index="740" style="margin-left:0;">(Marisa) Miniprepped and nanodropped pCIR 4, pBex 16 pBEX 19, CMV BFP-1, CMV GFP-1/2, 1945, 1946, 1947, 1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1450" data-index="741" style="margin-left:35px;">Grew up Bex 16 and Bex 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1451" data-index="742" style="margin-left:0;">(Jeffrey) Digested 1945, 1946, 1947, and 1948 with BbsI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1452" data-index="743" style="margin-left:35px;">Grew up pPV 1/5/6/7/8, 1942, 1943, 1944, and 1716</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1453" data-index="744" style="margin-left:0;">(Marisa) Grew up pCir 4 (x2), DC 56, BW 390, and BW 391</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1454" data-index="745" style="margin-left:35px;">Made LB + Carb </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 8/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1455" data-index="746" style="margin-left:0;">(Rachel and Kami) Ran FACS</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1456" data-index="747" style="margin-left:0;">(Kami) split HEK293 and stable line cells into big flasks</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1457" data-index="748" style="margin-left:0;">(Marisa) miniprep pPV 1, 5, 6, 7, 8, pBEX16, 19(x3), BW1942, BW1943, BW1944, BW1716</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1458" data-index="749" style="margin-left:35px;">    midipreped DC56, BW390, BW391, pCir4(x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1459" data-index="750" style="margin-left:0;">(Will) cut for gel extraction 1916, 1945, 1947, 1948 with BBs1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1460" data-index="751" style="margin-left:35px;">gel extracted those as well</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1461" data-index="752" style="margin-left:0;">(Will) grew up iGEM CMV for cell stock</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1462" data-index="753" style="margin-left:0;">(Rachel) annealed the mutated oligos</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 8/26</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1463" data-index="754" style="margin-left:0;">(Jeffrey and Marisa) Midiprep BW 2286, BW 2287, and pCir 4 (x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1464" data-index="755" style="margin-left:35px;">Cell stock CMV GFP-1</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1465" data-index="756" style="margin-left:0;">(Rachel) picked two colonies for pGOP 101-150</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1466" data-index="757" style="margin-left:0;">(Will) Gel extracted pBEX 16 and 19</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1467" data-index="758" style="margin-left:70px;">pBEX 16 digested with AscI and NheI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:70px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1468" data-index="759" style="margin-left:70px;">pBEX 19 digested with AscI and NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1469" data-index="760" style="margin-left:35px;">PCR overhang extension with sequences 37 and 56</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1470" data-index="761" style="margin-left:35px;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 8/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1471" data-index="762" style="margin-left:0;">(Kami, WIll, Rachel) Performed minipreps for Mismatches and additional multimerized operators pGOP 101-150</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 8/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1472" data-index="763" style="margin-left:0;">(Will) Test Cut for Mismatches </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1473" data-index="764" style="margin-left:0;">(Jeffrey) Attempted overhang extension PCR ... Failed</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 8/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1474" data-index="765" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) Attempted overhang extension PCR ... Failed</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1475" data-index="766" style="margin-left:0;">(Jeffrey) Completed Interlab Study</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1476" data-index="767" style="margin-left:0;">(Marisa)  PCR GEX plasmids for CAM transfer</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1477" data-index="768" style="margin-left:0;">(Will) Gel extracted PCR </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 8/31</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1478" data-index="769" style="margin-left:0;">(Marisa) Digested pGOP 109, 110, 112, 114, 134,l 135, 136, 138</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1479" data-index="770" style="margin-left:0;">(Marisa) Grew up pGEX 7, pGEX 8, pGPX1, </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1480" data-index="771" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) Attempted overhang extension PCR ... Failed</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1481" data-index="772" style="margin-left:0;">(Jeffrey) Test Cut  <span style="color:#344043"><span style="background-color:#FFFFFF">109, 110, 112, 114, 134,l 135, 136, 138</span></span></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1482" data-index="773" style="margin-left:0;">(will) pcr mruby and bfp to drop into pgops</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1483" data-index="774" style="margin-left:0;">(Jeffrey) MInipreped pgop 80-90 for multiplecolors , bexs for circuits, gexs f</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1484" data-index="775" style="margin-left:0;">(Marisa) Passaged HEK cells </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1485" data-index="776" style="margin-left:35px;">Made 2L of LB Broth, 2L of LB+Carb broth, and 2L of LB + Cam agar for plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1486" data-index="777" style="margin-left:35px;">Ligated and transformed pGEX 3, 5, 8, 10, 15, and 19 into Cam (with BW 1721 -n330 backbone)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1487" data-index="778" style="margin-left:35px;">Grew up T4o (x2), BW 363, GEX 5, GEX 15, pCir 4 (x2), and GPX 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1488" data-index="779" style="margin-left:0;">(Rachel) autoclaved epi tubes and tips</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1489" data-index="780" style="margin-left:35px;">Ligated and transformed BW 1945-1949</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1490" data-index="781" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1491" data-index="782" style="margin-left:0;">(Marisa) Made LB + Cam plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1492" data-index="783" style="margin-left:0;">(Jeffrey) Resuspended cells grown for midiprop and stored in the cold room</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1493" data-index="784" style="margin-left:0;">(Rachel) Prepared and ran cells for FACS</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1494" data-index="785" style="margin-left:0;">(Will) Annealed oligos for multimerized pGOP 3</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/3</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1495" data-index="786" style="margin-left:0;">(Jeffrey) transformed mismatched pGOP 101-150</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1496" data-index="787" style="margin-left:0;">(Marisa and Jeffrey) grew up GEX 3, 5, 8, 10, 15, 19, BW 1721, 1945, 1946, 1947, 1948,  and GOP 29</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/4</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1497" data-index="788" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) picked and grew up colonies from pGOP101-150</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1498" data-index="789" style="margin-left:35px;">miniprep of GEX 3, 5, 8, 10, 15, 19, BW 1721, 1945, 1946, 1947, 1948, and GOP 29</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1499" data-index="790" style="margin-left:35px;">Digested BW1945, 1946, 1947, 1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1500" data-index="791" style="margin-left:0;">(Marisa) Cell stock GOP 110, 112, and 114</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1501" data-index="792" style="margin-left:35px;">Digested GOP 29(MOP) with EcoRI and PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1502" data-index="793" style="margin-left:35px;">PCR GEX 3, 5, 8, 10, 15, 19 with PrA6 and PrA6r</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1503" data-index="794" style="margin-left:35px;">PCR clean up BW 1945, 1946, 1947, 1948 and MOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1504" data-index="795" style="margin-left:35px;">Made and ran gel for GEX 3, 5, 8, 10, 15, 19 and BW1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1505" data-index="796" style="margin-left:35px;">(with Kami) gel extracted GEX 3, 5, 8, 10, 15, 19, and BW 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1506" data-index="797" style="margin-left:35px;">Digested GEX 3, 5, 8, 10, 15, 19 and BW 1721 with EcoRI and PstI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1507" data-index="798" style="margin-left:35px;">PCR clean up GEX 3, 5, 8, 10, 15, 19, and BW 1721</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/5</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1508" data-index="799" style="margin-left:0;">(Marisa) Cell stock GOP 101-103; 105-108; 111; 115-134; and 146</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1509" data-index="800" style="margin-left:35px;">Miniprep pGOP 101-103; 105;108; 111; and 115-122</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1510" data-index="801" style="margin-left:35px;">Ligate and transformed pGEX 3, 5, 8, 10, 15, and 19 into pSB1C3 backbone</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1511" data-index="802" style="margin-left:0;">(Will) Miniprep pGOP 123-134 and 146</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1512" data-index="803" style="margin-left:0;">(Kami Rachel) seeded 8 48 well plates at 100,000cells for transfection on wednesday</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/6</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1513" data-index="804" style="margin-left:0;">(Marisa) picked two colonies of GEX 3, 5, 8, 10, 15, and 19 (now termed 103, 105, 108, 110, 115, and 119) from transformation 9/5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1514" data-index="805" style="margin-left:0;"><span style="color:#344043"><span style="background-color:#FFFFFF">(Jeffrey) Performed overhang extension pcr to build the decoder for circuit 2</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1515" data-index="806" style="margin-left:0;">(Jeffrey)  transformed the 18T entry vectors for circuit 5</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1516" data-index="807" style="margin-left:0;">(Marisa) miniprep GEXs 103, 105, 108, 110, 115, and 119</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1517" data-index="808" style="margin-left:35px;">Test cut with AseI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1518" data-index="809" style="margin-left:35px;">picked two colonies for 18T-terminator circuits </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1519" data-index="810" style="margin-left:0;">(Rachel, Marisa and Kami) transfected HEKcells</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1520" data-index="811" style="margin-left:0;">(kami Marisa) passaged HEK cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1521" data-index="812" style="margin-left:0;">(jeffrey) performed a gibson to build circuit 2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1522" data-index="813" style="margin-left:0;">(jeffrey) test cuts for 18T entry vecotrs for circuit 5 indicated low digestion efficeincy and the circuits needs to be retransformed</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1523" data-index="814" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1524" data-index="815" style="margin-left:0;"><b>FRIDAY 9/9</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1525" data-index="816" style="margin-left:0;">(Will) Ligated and transformed GEX 21-40 and GOP 151-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1526" data-index="817" style="margin-left:0;">(Will and Marisa) Grew up 3 colonies for GEX 21-40 and GOP 151-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1527" data-index="818" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1528" data-index="819" style="margin-left:0;"><b>Saturday 9/10</b></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1529" data-index="820" style="margin-left:0;">(Will Jeffrey Marisa) Miniprepped <span style="color:#344043"><span style="background-color:#FFFFFF">GEX 21-40 and GOP 151-168</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1530" data-index="821" style="margin-left:0;">(Marisa) PCR GEX 3, 5, 8, 10, 15, 19, and BW 1721 with PrA6 and PrA6r (previous GEX 103, 105 108, 110, 115, 119 were not successful)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1531" data-index="822" style="margin-left:0;">(Marisa and Will) gel extracted GEX 3, 5, 8, 10, 15, and 19 and BW 1721 (~400); will be insert for GEX 103, 105, 108, 110, 115, and 119</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1532" data-index="823" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/11</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1533" data-index="824" style="margin-left:0;">(Marisa) Digested GEX 103, 105, 108, 110, 115, and 119 and BW 1721 inserts with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1534" data-index="825" style="margin-left:0;">(Jeffrey) Picked colonies for 18T entry vectors and miniprepred circuit 2 for test cuts</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1535" data-index="826" style="margin-left:0;">(Will) Test cut <span style="color:#344043"><span style="background-color:#FFFFFF">GEX 21-40 and GOP 151-168</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1536" data-index="827" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/12</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1537" data-index="828" style="margin-left:0;">(Marisa) picked four colonies for 18T g1, g17, g3, g8, and g13</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1538" data-index="829" style="margin-left:35px;">Digested and test-cut 18T g1, g17, g3, g8, and g13 colonies 1 and 2 with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1539" data-index="830" style="margin-left:0;">(Marisa) Ligated and transformed inserts for GEX 3, 5, 8, 10, 15, and 19 into pSB1C3 backbone to create GEX 103, 105, 108, 110, 115, and 119  </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1540" data-index="831" style="margin-left:0;">(Kami) made 8 48 well plates with HEK cells at 100,000 cells/ml</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1541" data-index="832" style="margin-left:0;">(kami) retransformed pGOP7 because it looked wierd when we transfected it. so going to pick one colony from this transformation to try to start fresh. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1542" data-index="833" style="margin-left:0;">(Marisa) Made LB + Cam broth </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1543" data-index="834" style="margin-left:35px;">Grew up pSB1C3, GOP 35, and GPX2 for midipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1544" data-index="835" style="margin-left:35px;">Grew up pSB1C3 and GOP 45 (x2) for minipreps</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1545" data-index="836" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/13</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1546" data-index="837" style="margin-left:0;">(Marisa) picked two colonies for GEX 103, 105, 108, 110, 115, and 119</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1547" data-index="838" style="margin-left:35px;">Midiprep pSB1C3, pGPX2, pGOP35</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1548" data-index="839" style="margin-left:35px;">Miniprep GOP45 (x2), SB1C3, and g17 18T-1/2/3/4</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1549" data-index="840" style="margin-left:35px;">made master mixes for transfection to take place on 9/14</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1550" data-index="841" style="margin-left:35px;">picked and grew up a colony from GOP7 re-transformation</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1551" data-index="842" style="margin-left:35px;">Added Carbacillin to plain LB Broth to make LB+Carb broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1552" data-index="843" style="margin-left:35px;">Grew up Cir4 (x2), SB1C3 (x2), GEX5, GEX 15, GPX2, T40 (x2), and BW471 </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/14</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1553" data-index="844" style="margin-left:0;">(Marisa) miniprepped pGEX 103-1/2, 105-1/2, 108-1/2, 110-1/2, 115-1/2, and 119-1/2</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1554" data-index="845" style="margin-left:35px;">Test cut GEXs with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1555" data-index="846" style="margin-left:35px;">Test Cut g17 18T with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1556" data-index="847" style="margin-left:0;">(Marisa and Jeffrey) made 2L of plain LB broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1557" data-index="848" style="margin-left:0;">(Jeffrey) midiprep pSB1C3 (x2), T40(x2), and pCir4(x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1558" data-index="849" style="margin-left:0;">(Kami Rachel) transfected Hek cells. with pGOPs diluted to 5ng/ul (not 50ng/ul) and add a bigger volume to the transfection mix. </div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1559-item" data-formattable="true" data-index="850" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.43.39.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="JEeap2VgHjzbtoJ48RyQsp5aweyRHe3V5g8lVTUC-Screen_Shot_2016-09-15_at_01.43.39.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1559" data-index="850">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1561-item" data-formattable="true" data-index="851" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.43.46.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="7elqO9a2oEa1WuI28OtvtR3luaswiUZ95EB6cI2l-Screen_Shot_2016-09-15_at_01.43.46.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1561" data-index="851">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1563-item" data-formattable="true" data-index="852" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.44.02.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Gevqn5XtZwrpeRO5ANTKcMNUtU8zZKFxAl2QxYrG-Screen_Shot_2016-09-15_at_01.44.02.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1563" data-index="852">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1565-item" data-formattable="true" data-index="853" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.44.20.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="735677Efcro0ZK7rsHbSwcRzzxewxT5tswpeTaPP-Screen_Shot_2016-09-15_at_01.44.20.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1565" data-index="853">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1567-item" data-formattable="true" data-index="854" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.44.29.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="Lc9mxtOSzkCpHAMy7XbTlzIIYwaRoGtAXbCxh9bY-Screen_Shot_2016-09-15_at_01.44.29.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1567" data-index="854">
-</div></div></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1569-item" data-formattable="true" data-index="855" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-09-15 at 01.45.21.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="b56gbrHOLk79LJnXldGESIxsg5XryN1VJGKce8ND-Screen_Shot_2016-09-15_at_01.45.21.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1569" data-index="855">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/15</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1571" data-index="856" style="margin-left:0;">(Marisa) Miniprep GEX 103-3/4/5/6, 105-3/4/5/6, 108-3/4/5/6, 110-3/4/5/6, 115-3/4/5/6, 119-3/4/5/6</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1572" data-index="857" style="margin-left:35px;">Digest and test cut GEXs with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1573" data-index="858" style="margin-left:0;">(Marisa and Kami) passaged stable line HEK cells (Wild Type, 2578, and 2579) 1:20</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1574" data-index="859" style="margin-left:0;">(Jeffrey) Minipreped 1945-1948</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/16</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1575" data-index="860" style="margin-left:0;">(Kami) Miniprep pGOP 159-1/2/3, 160-1/2/3, 169-1/2/3, and 170-1/2/3</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1576" data-index="861" style="margin-left:0;">(Will) Test cut pGOP 159, 160, 169, and 170</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 9/17</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1577" data-index="862" style="margin-left:0;">(jeffrey) ligated and transformed BEX34-38</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/18</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1578" data-index="863" style="margin-left:0;">(Marisa and Jeffrey) picked three colonies of GOP 151-158 and 161-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1579" data-index="864" style="margin-left:0;">(Jeffrey) Picked colonies for BEX37 Redigested 1945,1946,1948</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1580" data-index="865" style="margin-left:0;">(Jeffrey) Religated and transformed BEX 34,35,36,38</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/19</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1581" data-index="866" style="margin-left:0;">(Marisa) Miniprep colonies 1,2, and 3 of GOP 151-158 and 161-168</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1582" data-index="867" style="margin-left:0;">(Jeffrey) PIcked colonies for BEX 34-38</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1583" data-index="868" style="margin-left:0;">(Kami) Made 6 hek plates at 100,000  (NOT USED)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1584" data-index="869" style="margin-left:0;">(Will) Test cut the miniprepped GOPs 151-168</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/20</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1585" data-index="870" style="margin-left:0;">(Marisa) Cell stock BEX 30, GOP 159, 160, 169</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1586" data-index="871" style="margin-left:0;">(Marisa) made 2L of plain LB broth</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1587" data-index="872" style="margin-left:0;">(Marisa) Diluted GOP 81-90 to 25 ng/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1588" data-index="873" style="margin-left:0;">(jeffrey) Minipreppred bex 34-38 and test cut</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1589" data-index="874" style="margin-left:0;">(Jeffrey) Digested BEX 1,6,15,20, 30,31,32,33</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1590" data-index="875" style="margin-left:0;">(Jeffrey) digested parts vectors with bbsi for 3 input circuit</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1591" data-index="876" style="margin-left:0;">(Will) Sent in colony 1 of GOPs 151-168 that test cut correctly</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/21</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1592" data-index="877" style="margin-left:0;">(Marisa and Rachel) Made LB agar (x2)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1593" data-index="878" style="margin-left:0;">(Marisa) Cell stock pGOP 159, 160, and 169</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1594" data-index="879" style="margin-left:0;">(Marisa and Kami) Ligated GOP 153, 154, 157, 163, 165, 166, 168, 170, 162, and 167 with respective oigos</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1595" data-index="880" style="margin-left:0;">(rachel) transformed the pGOPs ligated above</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1596" data-index="881" style="margin-left:0;">(Kami) Made LB + Cam plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1597" data-index="882" style="margin-left:0;">(Marisa) Made LB + Carb plates</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1598" data-index="883" style="margin-left:0;">(Jeffrey) Gibson Cir2 and Cir 5 using BEX parts vectors</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/22</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1599" data-index="884" style="margin-left:0;">(Kami) picked 4 colonies for GOP 153, 154, 157, 162, 163, 165, 166, 167, 168, 170</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1600" data-index="885" style="margin-left:0;">(Jeffrey) Cell stock GOP 151, 152, 155, 156, 158, 161, 164 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1601" data-index="886" style="margin-left:35px;">Miniprepped colonies 5-8 of GOP <span style="color:#344043"><span style="background-color:#FFFFFF">153, 154, 157, 162, 163, 165, 166, 167, 168, 170</span></span></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1602" data-index="887" style="margin-left:35px;">Test cut GOPs with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1603" data-index="888" style="margin-left:35px;">Digested BEX 34-37  with AscI and NotI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1604" data-index="889" style="margin-left:35px;">Digested BEX 38 with AscI and NheI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1605" data-index="890" style="margin-left:0;">(Kami) passaged HEK cells</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1606" data-index="891" style="margin-left:0;">(Will)  Sent in colony two of GOPs 151-168 that did not sequence properly</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1607" data-index="892" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/23</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1608" data-index="893" style="margin-left:0;">Cell stocked pGOP 154, </div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 9/25</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1609" data-index="894" style="margin-left:0;">(Rachel) Ligated and transformed 1721 insert into MOP (GOP with biobrick cut out)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1610" data-index="895" style="margin-left:0;">(Jeffrey) Gibson Circuit 2 and 5</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Monday, 9/26</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1611" data-index="896" style="margin-left:0;">(Marisa) miniprepped four colonies of pGOP 104, 109, 135, 137, 144, 149, and 150</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1612" data-index="897" style="margin-left:35px;">Test cut GOP (x3) of 91-100, GOP (x4) 104, 109, 135, 137, 144, 149, 150, 153, 154, 157, 162, 163, 165, 166, 167, 168, 170, and GOP (x3) 113, 135, 136, 138, 142, with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1613" data-index="898" style="margin-left:0;">(Marisa) Transformed Circuit 2 and Circuit 5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1614" data-index="899" style="margin-left:0;">(Marisa) picked four colonies of MOP + 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1615" data-index="900" style="margin-left:0;">(Will) Sent in GOP151 and 163 for sequencing</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1616" data-index="901" style="margin-left:0;">(Kami) made HEK plates</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Tuesday, 9/27</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1617" data-index="902" style="margin-left:0;">(Marisa) PCR pGEX 3, 8, 15, and 19 and BW 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1618" data-index="903" style="margin-left:0;">(Marisa and Will) Gel extracted pGEX 3, 8, 15, 19 and BW 1721</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1619" data-index="904" style="margin-left:0;">(Will) Digested GEX 3, 8, 15, 19 and BW 1721 with EcoRI-HF and PstI-HF</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1620" data-index="905" style="margin-left:35px;">PCR cleanup of GEXs and BW1721 </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1621" data-index="906" style="margin-left:0;">(Rachel) Ligated GEX 3, 8, 15, 19, and BW 1721 with MOP</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1622" data-index="907" style="margin-left:0;">(Marisa) Transformed GEX 3, 8, 15, 19 and BW 1721 (to GEX 103, 108, 115, and 119)</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1623" data-index="908" style="margin-left:0;">(Will) Cell stocked pGOP 151 and 163</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Wednesday, 9/28</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1624" data-index="909" style="margin-left:0;">(Marisa) Test cut GOP 162, 163, and 167 colonies 5-8 with BbsI and SpeI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1625" data-index="910" style="margin-left:0;">(Marisa) Cell stock 91-100, 104, 109, 113, 136, 137, 138, 142, 144, 149, 150, 153, and 157</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1626" data-index="911" style="margin-left:0;">(Marisa) miniprep GOP 112, 114, 135, </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1627" data-index="912" style="margin-left:0;">(Marisa) Replated Circuit 2 and 5</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Thursday, 9/29</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1628" data-index="913" style="margin-left:0;">(Marisa) Cell stock pGOP 151 and 163</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1629" data-index="914" style="margin-left:0;">(Marisa) Replated Circuit 2 and Circuit 5</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1630" data-index="915" style="margin-left:0;">(Marisa and Rachel) ligated and transformed pGOP 142, 162, and 167</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 9/30</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1631" data-index="916" style="margin-left:0;">(Marisa) picked 5 colonies of pGOP 167</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1632" data-index="917" style="margin-left:0;">(Kami) made 11 hek plates at 100,000cells/ul</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1633" data-index="918" style="margin-left:0;">(kami)Diluted pGOPs to 25ng/ul</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 10/1</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1634" data-index="919" style="margin-left:0;">(Marisa) Miniprep and test cut pGOP 167-1/2/3/4/5 with BbsI and SpeI</div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 10/2</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1635" data-index="920" style="margin-left:0;">(Kami Rachel) Diluted DNA for transfection. </div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1636" data-index="921" style="margin-left:0;">(Kami Rachel) Transfected hek cells with all the multimerized and mutated pGOPs</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:35px;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1637" data-index="922" style="margin-left:35px;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1638-item" data-formattable="true" data-index="923" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.03.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="pPGDrwkHDdZI8My8HLfC7fuIGWvUanttaSvsxIs3-Screen_Shot_2016-10-10_at_11.34.03.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1638" data-index="923">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1640" data-index="924" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1641-item" data-formattable="true" data-index="925" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.15.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="yB64ra9qxRfhK1xwX5bjOvBPrl5pb3eM5QlMfP5e-Screen_Shot_2016-10-10_at_11.34.15.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1641" data-index="925">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1643" data-index="926" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1644-item" data-formattable="true" data-index="927" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.25.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="MQFs5Y7UrPnIGabfCi1yaDFzk2vyELbrTFjkVilu-Screen_Shot_2016-10-10_at_11.34.25.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1644" data-index="927">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1646" data-index="928" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1647-item" data-formattable="true" data-index="929" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.34.37.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="i5ZQqz1LHKinEmYksDWlwIN4BSGJAsaUAPQ63dAA-Screen_Shot_2016-10-10_at_11.34.37.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1647" data-index="929">
-</div></div></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Friday, 10/7</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1649" data-index="930" style="margin-left:0;">(kami) made 7 hek plates at 100,000 for transfection on sunday and 1 hek plate at 200,000 cells/ml for transfection on saturday with WPI</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1650" data-index="931" style="margin-left:0;">(Rachel) prepared the transfection DNA mix for WPI</div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1651-item" data-formattable="true" data-index="932" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.30.05.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="SMbeGz3C71A7aXcgKue8BXssQiBDMkSFA5CRbx7B-Screen_Shot_2016-10-10_at_11.30.05.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1651" data-index="932">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1653" data-index="933" style="margin-left:0;"></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1654" data-index="934" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Saturday, 10/8</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1655" data-index="935" style="margin-left:0;">(Kami + Rachel) transfected cells for WPI testing the and/nor circuit</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1656" data-index="936" style="margin-left:0;">(Kami + Rachel) prepared all the DNA mixes for transfection tomorrow</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1657" data-index="937" style="margin-left:0;"></div></div></div><div class="daySeparator"><div class="daySeparator-inputWrapper"><input class="daySeparator-inputWrapper-hiddenInput" type="input"/></div><span class="daySeparator-date">Sunday, 10/9</span><div class="daySeparator-line"></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1658" data-index="938" style="margin-left:0;">(Kami) Transfected HEK cells with screen of mutated p GOPs, CMV compared to single and triple multimerized, and 4 analog circuits</div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1659" data-index="939" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1660-item" data-formattable="true" data-index="940" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.32.13.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="35zz4Sc5nei3ggaIX5VH7t2s3wXjf8oXDAfQtvP9-Screen_Shot_2016-10-10_at_11.32.13.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1660" data-index="940">
-</div></div></div></div></div><div class="mediocre-item is-text"><div class="mediocre-noteEditable multi-editable is-paragraph"><div class="mediocre-noteEditable-indenter" style="width:0;"></div><div class="editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="false" data-cid="note-part1662" data-index="941" style="margin-left:0;"></div></div></div><div class="mediocre-item"><div style="margin-left:0;"><div class="hiddenFocusEditable editable" contenteditable="true" data-cid="note-part1663-item" data-formattable="true" data-index="942" data-subindex="item"></div><div class="mediocre-captionEditable multi-editable"><div class="component-top-row component-row"><div class="mediocre-captionEditable-cell mediocre-captionEditable-component"><div class="note-item-wrapper"><div class="dnd-drop-container"><div class="noteLinkedFilePreview mediocre-itemSelect note-item"><div><h5 class="u-no-spacing-top u-no-spacing-bottom"><div class="pull-right"><a class="previewCTA" target="_blank"><span class="glyphicon glyphicon-new-window" icon="new-window"></span></a></div><span class="glyphicon glyphicon-paperclip note-icon u-small-spacing-right pull-left" icon="paperclip"></span><div class="note-itemName">Screen Shot 2016-10-10 at 11.32.30.png</div></h5><div class="note-preview-body"><img alt="thumbnail" class="noteLinkedFilePreviewImg" src="xSZzysovfvKFMuRanfkPKo7yIih9fxQr5Qt1IfMa-Screen_Shot_2016-10-10_at_11.32.30.png"/></div></div></div><div class="dnd-drop-container-border"></div></div></div></div><div class="mediocre-captionEditable-cell" style="width:0;"></div><div class="mediocre-captionEditable-cell multi-editable mediocre-captionEditable-caption editable selectable is-locked" contenteditable="false" data-allow-at-search="true" data-allow-multiline="true" data-cid="note-part1663" data-index="942">
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