Difference between revisions of "Team:HUST-China/Composite Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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                        <li><a href="https://2016.igem.org/Team:HUST-China">HOME</a></li>
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                        <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/project/background">Background</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Description">Description</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Proof">Proof of concept</a></li>
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<div class="column full_size">
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Demonstrate">Application</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/project/inprovement">Previous improvement</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Safety">Safety</a></li>
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                            </ul>
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<p>
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                        </li>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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                        <li class="dropdown1"><a href="#" class="down-scroll">WETLAB</a>
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</p>
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                            <ul class="dropdown2">
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Experiments">Experiments</a></li>
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Results">Results</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/InterLab">Interlab</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Notebook">Notebook</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li>
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<div class="highlight">
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                            </ul>
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<h4>Note</h4>
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                        </li>
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<p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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                        <li class="dropdown1"><a class="down-scroll" href="https://2016.igem.org/Team:HUST-China/Model">MODELING</a>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-pro">Prokaryotic circuit</a></li>
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</div>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-euk">Eukaryotic circuit</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-app">Application circuit</a></li>
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                            </ul>
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                        </li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Basic_Part">Basic part</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Composite_Part">Composite part</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li>
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                            </ul>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Integrated_Practices">Integrated HP</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li>
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                            </ul>
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                        </li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Team">Team Roster</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
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                            </ul>
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                        </li>
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            <h2 class="text-center">Basic Part</h2>
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            <p style="color:#fff;margin-bottom:10px;text-align:center;">Following documented composite parts are respectively from three circuits this year:</p>
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            <div class="ref"></div>
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            <article>
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                <h2>Prokaryotic version of Signal Filter:</h2>
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                <img src="https://static.igem.org/mediawiki/2016/8/88/T--HUST-China--Description-Fig-Eukaryote.png" alt="">
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                <h3>Protein& promoter interaction:</h3>
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                <img src="https://static.igem.org/mediawiki/2016/0/06/T--HUST-China--Experiments-Fig10.png" alt="">
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                <table border="1">
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                    <tr>
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                        <td>Part Number</td>
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                        <td>Name</td>
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                        <td>Description</td>
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                    </tr>
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                    <tr>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2036013"> BBa_K2036013 </a></td>
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                        <td> RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag </td>
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                        <td> To characterize CII’s interaction with pRE.</td>
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                    </tr>
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                    <tr>
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                        <td><a href="http://parts.igem.org/Part:BBa_K2036015"> BBa_K2036015 </a></td>
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                        <td> RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag </td>
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                        <td> Tandem of CII amplifies its function.</td>
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                    </tr>
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                </table>
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                <h3>Protein&protein interaction:</h3>
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                <img src="https://static.igem.org/mediawiki/2016/f/f9/T--HUST-China--Experiments-Fig6.png" alt="">
 +
                <table border="1">
 +
                    <tr>
 +
                        <td>Part Number</td>
 +
                        <td>Name</td>
 +
                        <td>Description</td>
 +
                    </tr>
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                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2036014"> BBa_K2036014 </a></td>
 +
                        <td> RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag </td>
 +
                        <td> To characterize CIII’s interaction with Ftsh.</td>
 +
                    </tr>
 +
                </table>
 +
                <h3>Tri-stable function:</h3>
 +
                <img src="https://static.igem.org/mediawiki/2016/1/17/T--HUST-China--Experiments-Fig14.png" alt="">
 +
                <table border="1">
 +
                    <tr>
 +
                        <td>Part Number</td>
 +
                        <td>Name</td>
 +
                        <td>Description</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2036020"> BBa_K2036020 </a></td>
 +
                        <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td>
 +
                        <td> To construct the verification circuit.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2036027"> BBa_K2036027 </a></td>
 +
                        <td>pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td>
 +
                        <td> To verify the function of the whole circuit with T7 and ptrp sensing the signals.</td>
 +
                    </tr>
 +
                </table>
 +
                <h2>Eukaryotic version of Signal Filter:</h2>
 +
                <img src="https://static.igem.org/mediawiki/2016/1/1e/T--HUST-China--Description-Fig-prokaryote.png" alt="">
 +
                <h3>Bi-stable-function</h3>
 +
                <img src="https://static.igem.org/mediawiki/2016/4/45/T--HUST-China--Experiments-Fig4-1.png" alt="">
 +
                <img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt="">
 +
                <table border="1">
 +
                    <tr>
 +
                        <td>Part Number</td>
 +
                        <td>Name</td>
 +
                        <td>Description</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2036026"> BBa_K2036026 </a></td>
 +
                        <td> PP2CA-TTADH1 </td>
 +
                        <td> To construct the verification circuit.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2036033"> BBa_K2036033 </a></td>
 +
                        <td> PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc </td>
 +
                        <td> To construct the verification circuit. </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2036029"> BBa_K2036029 </a></td>
 +
                        <td>PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag </td>
 +
                        <td> To characterize PP2CA’s interaction with ABF2,which means the whole circuit functional verification. </td>
 +
                    </tr>
 +
                </table>
 +
                <h2>Application of Signal Filter:</h2>
 +
                <img src="https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--modeling-application.png" alt="">
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                <table border="1">
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                    <tr>
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                        <td>Part Number</td>
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                        <td>Name</td>
 +
                        <td>Description</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td><a href="http://parts.igem.org/Part:BBa_K2036024"> BBa_K2036024 </a></td>
 +
                        <td> placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase </td>
 +
                        <td> The whole circuit of the application for lactose intolerance. </td>
 +
                    </tr>
 +
                </table>
 +
            </article>
 +
        </div>
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Revision as of 17:23, 15 October 2016

Parts

Basic Part

Following documented composite parts are respectively from three circuits this year:

Prokaryotic version of Signal Filter:

Protein& promoter interaction:

Part Number Name Description
BBa_K2036013 RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CII’s interaction with pRE.
BBa_K2036015 RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag Tandem of CII amplifies its function.

Protein&protein interaction:

Part Number Name Description
BBa_K2036014 RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CIII’s interaction with Ftsh.

Tri-stable function:

Part Number Name Description
BBa_K2036020 RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To construct the verification circuit.
BBa_K2036027 pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To verify the function of the whole circuit with T7 and ptrp sensing the signals.

Eukaryotic version of Signal Filter:

Bi-stable-function

Part Number Name Description
BBa_K2036026 PP2CA-TTADH1 To construct the verification circuit.
BBa_K2036033 PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc To construct the verification circuit.
BBa_K2036029 PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag To characterize PP2CA’s interaction with ABF2,which means the whole circuit functional verification.

Application of Signal Filter:

Part Number Name Description
BBa_K2036024 placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase The whole circuit of the application for lactose intolerance.