Difference between revisions of "Team:BostonU/Basic Part"
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<a href = "http://parts.igem.org/Part:BBa_K1875012" style = "color:blue;">BBa_K1875012</a></p> | <a href = "http://parts.igem.org/Part:BBa_K1875012" style = "color:blue;">BBa_K1875012</a></p> | ||
Revision as of 19:54, 15 October 2016
To create the Gemini parts library, we made use of two “classes” of plasmid: guide RNA expressing vectors and guide RNA operator reporter vectors. When these two plasmids are co-transfected with a dCas9-VPR plasmid into a mammalian cell line, the expression of a gene of interest proceeds. Our work for iGEM dealt with fluorescent proteins; specifically, GFP for the parts submitted to the registry. The guide RNA expressing vector can be considered a basic part. Its design consists of a hu6 promoter upstream of both the guide RNA sequence as well as the spCas9 scaffold. This basic part, whose data can be found on BBa_K1875012, was based off of the work by Feng Zhang at MIT and the x330 plasmid. It represents what we feel is one of best basic parts in our library due to its consistency in experimentation.
