Difference between revisions of "Team:BostonU/Parts"
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| − | <br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;">In addition to the Bronze Medal parts, we submitted a selection of guide RNA operator reporters for Silver Medal consideration. | + | <br><p style = "font-size:150%; padding:25px 150px 20px 150px; color:#0071A7;">In addition to the Bronze Medal parts, we submitted a selection of our guide RNA operator reporters as composite parts for<a href = "https://2016.igem.org/Team:BostonU/Medal_Criteria" style = "color:blue;">Silver Medal consideration</a>. We chose to submit the four single site operators that |
| + | compose our circuit: <a href = "http://parts.igem.org/Part:BBa_K1875013" style = "color:blue;">BBa_K1875013</a>, <a href = "http://parts.igem.org/Part:BBa_K1875014" style = "color:blue;">BBa_K1875014</a>, <a href = "http://parts.igem.org/Part:BBa_K1875015" style = "color:blue;">BBa_K1875015</a>, <a href = "http://parts.igem.org/Part:BBa_K1875016" style = "color:blue;">BBa_K1875016</a>. We also submitted out strongest double and triple mulitmerizered operators, <a href = "http://parts.igem.org/Part:BBa_K1875017" style = "color:blue;">BBa_K1875017</a> and <a href = "http://parts.igem.org/Part:BBa_K1875018" style = "color:blue;">BBa_K1875018</a>, respectively. Finally, we submitted our lowest expressing mutant operator, <a href = "http://parts.igem.org/Part:BBa_K1875019" style = "color:blue;">BBa_K1875019</a>. All of these operators drive a GFP reporter. | ||
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| − | + | Because our parts for our Silver Medal requirement are considered composite parts, we needed to create a collection of basic parts pages on the BioBrick Registry. These parts include the minimal CMV promoter in our operators, a Kozak sequence, a Rabbit Beta Globin Poly A terminator, a green fluorescent protein, and the target sequences for our expression vectors and operator reporters. These parts have the ID numbers <a href = "http://parts.igem.org/Part:BBa_K1875000" style = "color:blue;">BBa_K1875000</a> through <a href = "http://parts.igem.org/Part:BBa_K1875010" style = "color:blue;">BBa_K1875010</a>.These parts do not have samples in the registry; however, their sequences and additional information can be found on their respective Registry pages. </p> | |
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Revision as of 21:16, 15 October 2016
Welcome to the BostonU Parts Pages. We will be brief here but if you would like more detailed information on the parts associated with Gemini we recommend you visit our various pages on the iGEM BioBrick Registry.
We submitted several parts from our Gemini Library to the registry, including two of our guide RNA expression vectors as basic parts for our Bronze Medal requirement. These vectors contain the target sequences for guide RNA 3 and guide RNA 8 respectively. BBa_K1875011 expresses g3, a guide RNA that directly recognizes the 20bp target sequence 5’-AATGAACCTATTCGTACCGT-3’. This sequence can be found in part BBa_K1875004. BBa_K1875012 expresses g8, a guide RNA that directly recognizes the 20bp target sequence 5’-GTTGCGCGTCCGTATCAAGG-3’. This sequence can be found in part BBa_K1875006.
In addition to the Bronze Medal parts, we submitted a selection of our guide RNA operator reporters as composite parts forSilver Medal consideration. We chose to submit the four single site operators that compose our circuit: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016. We also submitted out strongest double and triple mulitmerizered operators, BBa_K1875017 and BBa_K1875018, respectively. Finally, we submitted our lowest expressing mutant operator, BBa_K1875019. All of these operators drive a GFP reporter.
Because our parts for our Silver Medal requirement are considered composite parts, we needed to create a collection of basic parts pages on the BioBrick Registry. These parts include the minimal CMV promoter in our operators, a Kozak sequence, a Rabbit Beta Globin Poly A terminator, a green fluorescent protein, and the target sequences for our expression vectors and operator reporters. These parts have the ID numbers BBa_K1875000 through BBa_K1875010.These parts do not have samples in the registry; however, their sequences and additional information can be found on their respective Registry pages.
