Difference between revisions of "GDSYZX-United/PROJECT/enzyme cleavage"

 
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<p><div class="text_font"><strong>Place:</strong>Biology Experiment Teaching Center Of Sun Yat-sen University</div></p>
 
<p><div class="text_font"><strong>Place:</strong>Biology Experiment Teaching Center Of Sun Yat-sen University</div></p>
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<p><div class="text_font"><strong>Date:</strong>July 27th, 2016</div></p>
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<p><div class="text_font"><strong>Experimental drugs: </strong>PCR products, Bsa1 restriction enzyme,Pst1 restriction enzyme,Ecor1  restriction enzyme,Not1 restriction enzyme,10×buffer solution, ddH2O</div></p>
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<p><div class="text_font"><strong>Experimental drugs: </strong>PCR products, Pst1restriction enzyme,10×buffer solution, ddH2O</div></p>
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Latest revision as of 06:18, 16 October 2016

Enzyme cleavage

Place:Biology Experiment Teaching Center Of Sun Yat-sen University

Experimental drugs: PCR products, Bsa1 restriction enzyme,Pst1 restriction enzyme,Ecor1 restriction enzyme,Not1 restriction enzyme,10×buffer solution, ddH2O

Procedure:

  1. enzyme cleavage:configuration
    PCR products (DNA) 5 μl
    10×buffer solution 10 μl
    Pst1restriction enzyme 0.8μl
    ddH2O 84.2 μl
    Blending, keeping in 37℃ for 1.5-3h

  2. electrophoresis
    Take out 5μ DNA after enzyme cleavage to electrophoresis.