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|
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| <div style="padding-left:32px;"><li>pMV-G19 and pMV-G15 containing our target genes were received.</li><br/> | | <div style="padding-left:32px;"><li>pMV-G19 and pMV-G15 containing our target genes were received.</li><br/> |
| | | |
| + | <a href="https://static.igem.org/mediawiki/2016/6/6d/Igem-6803-week3-1.jpg" data-lightbox="no" data-title="pMV-G19 and pMV-G15"> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/6/6d/Igem-6803-week3-1.jpg" alt="Igem-6803-week3-1" width="300px" ><br/><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/6/6d/Igem-6803-week3-1.jpg" alt="Igem-6803-week3-1" width="300px" ><br/><br/> |
| </a> | | </a> |
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| <li>The enzyme-digested product was dephosphorylation.</li> | | <li>The enzyme-digested product was dephosphorylation.</li> |
| <br/> | | <br/> |
| + | |
| + | <a href="https://static.igem.org/mediawiki/2016/a/aa/Igem-6803-week3.png" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/a/aa/Igem-6803-week3.png" alt="Igem-6803-week3-2" width="800" height="533"><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/a/aa/Igem-6803-week3.png" alt="Igem-6803-week3-2" width="800" height="533"><br/> |
| </a> | | </a> |
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| Two of the successful ones were used to inoculate overnight cultures.<br/> | | Two of the successful ones were used to inoculate overnight cultures.<br/> |
| <br/> | | <br/> |
| + | |
| + | <a href="https://static.igem.org/mediawiki/2016/8/81/Igem-6803-week4.png" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/8/81/Igem-6803-week4.png" alt="Igem-6803-week4" width="800" height="533"> <br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/8/81/Igem-6803-week4.png" alt="Igem-6803-week4" width="800" height="533"> <br/> |
| </a> | | </a> |
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| <b>The fourth and sixth ones were not successful.</b><br/> | | <b>The fourth and sixth ones were not successful.</b><br/> |
| <br/> | | <br/> |
| + | |
| + | <a href="https://static.igem.org/mediawiki/2016/4/40/Igem-6803-week5.png" data-lightbox="no" data-title=" "> |
| <img align="center"src="https://static.igem.org/mediawiki/2016/4/40/Igem-6803-week5.png" alt="Igem-6803-week5" width="800" height="533" ><br/> | | <img align="center"src="https://static.igem.org/mediawiki/2016/4/40/Igem-6803-week5.png" alt="Igem-6803-week5" width="800" height="533" ><br/> |
| </a> | | </a> |
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| <li>Restriction digest on pCPC-3031-Ni with Sac I.</li> | | <li>Restriction digest on pCPC-3031-Ni with Sac I.</li> |
| <br/> | | <br/> |
| + | <a href="https://static.igem.org/mediawiki/2016/8/89/Igem-6803-week6-2.jpg" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/8/89/Igem-6803-week6-2.jpg" alt="Igem-6803-week6-1" width="300px"><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/8/89/Igem-6803-week6-2.jpg" alt="Igem-6803-week6-1" width="300px"><br/> |
| <br/> | | <br/> |
| <li>The fragment <i>13-19-15</i> was ligated onto T vector and transformed into E.coli via heat shock.</li> | | <li>The fragment <i>13-19-15</i> was ligated onto T vector and transformed into E.coli via heat shock.</li> |
| <br/> | | <br/> |
| + | <a href="https://static.igem.org/mediawiki/2016/0/06/Igem-6803-week6.jpg" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/0/06/Igem-6803-week6.jpg" alt="Igem-6803-week6-2" width="300px" ><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/0/06/Igem-6803-week6.jpg" alt="Igem-6803-week6-2" width="300px" ><br/> |
| <br/> | | <br/> |
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| <li>Three colonies were proved to be true. And two of them were used to inoculate overnight cultures.</li> | | <li>Three colonies were proved to be true. And two of them were used to inoculate overnight cultures.</li> |
| <br/> | | <br/> |
| + | <a href="https://static.igem.org/mediawiki/2016/2/2d/Igem-6803-week6-3.png" data-lightbox="no" data-title=" "> |
| <img align="center" src="https://static.igem.org/mediawiki/2016/2/2d/Igem-6803-week6-3.png" alt="Igem-6803-week6-3" width="800" height="533" ><br/> | | <img align="center" src="https://static.igem.org/mediawiki/2016/2/2d/Igem-6803-week6-3.png" alt="Igem-6803-week6-3" width="800" height="533" ><br/> |
| <br/> | | <br/> |