Difference between revisions of "Team:HUST-China/Composite Part"

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                         <td><a href="http://parts.igem.org/Part:BBa_K2036024"> BBa_K2036024 </a></td>
 
                         <td><a href="http://parts.igem.org/Part:BBa_K2036024"> BBa_K2036024 </a></td>
 
                         <td> placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase </td>
 
                         <td> placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase </td>
−
                         <td> The whole circuit of the application for lactose intolerance. </td>
+
                         <td> The whole circuit of the application for lactose intolerance therapy. </td>
 
                     </tr>
 
                     </tr>
 
                 </table>
 
                 </table>

Revision as of 12:26, 16 October 2016

Parts

Composite Part

Following documented composite parts are respectively from three circuits this year:

Prokaryotic version of Signal Filter:

Protein& promoter interaction:

Part Number Name Description
BBa_K2036013 RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CII’s interaction with pRE.
BBa_K2036015 RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag Tandem of CII amplifies its function.

Protein&protein interaction:

Part Number Name Description
BBa_K2036014 RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CIII’s interaction with Ftsh.

Tri-stable function:

Part Number Name Description
BBa_K2036020 RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To construct the verification circuit.
BBa_K2036027 pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To verify the function of the whole circuit with T7 and ptrp sensing the signals.

Eukaryotic version of Signal Filter:

Bi-stable-function

Part Number Name Description
BBa_K2036026 PP2CA-TTADH1 To construct the verification circuit.
BBa_K2036033 PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc To construct the verification circuit.
BBa_K2036029 PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag To characterize PP2CA’s interaction with ABF2,which means the whole circuit functional verification.

Application of Signal Filter:

Part Number Name Description
BBa_K2036024 placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase The whole circuit of the application for lactose intolerance therapy.