Difference between revisions of "Team:Tianjin/Parts"
| Line 209: | Line 209: | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
<td style="width:60%;text-align:left">PETase site-directed mutant A240P</td> | <td style="width:60%;text-align:left">PETase site-directed mutant A240P</td> | ||
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr> | </tr> | ||
<tr style="width:100%"> | <tr style="width:100%"> | ||
<td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110101" target="_blank">BBa_K2110101</a></td> | <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110101" target="_blank">BBa_K2110101</a></td> | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
| − | <td style="width:60%;text-align:left">PETase site-directed mutant | + | <td style="width:60%;text-align:left">PETase site-directed mutant S207T</td> |
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr> | </tr> | ||
<tr style="width:100%"> | <tr style="width:100%"> | ||
| Line 221: | Line 221: | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
<td style="width:60%;text-align:left">PETase site-directed mutant M154L</td> | <td style="width:60%;text-align:left">PETase site-directed mutant M154L</td> | ||
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr> | </tr> | ||
<tr style="width:100%"> | <tr style="width:100%"> | ||
| Line 227: | Line 227: | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
<td style="width:60%;text-align:left">PETase site-directed mutant N205V</td> | <td style="width:60%;text-align:left">PETase site-directed mutant N205V</td> | ||
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr> | </tr> | ||
<tr style="width:100%"> | <tr style="width:100%"> | ||
| Line 233: | Line 233: | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
<td style="width:60%;text-align:left">PETase site-directed mutant R90A</td> | <td style="width:60%;text-align:left">PETase site-directed mutant R90A</td> | ||
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr><tr style="width:100%"> | </tr><tr style="width:100%"> | ||
<td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110105" target="_blank">BBa_K2110105</a></td> | <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110105" target="_blank">BBa_K2110105</a></td> | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
| − | <td style="width:60%;text-align:left">PETase site-directed mutant | + | <td style="width:60%;text-align:left">PETase site-directed mutant I208V</td> |
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr><tr style="width:100%"> | </tr><tr style="width:100%"> | ||
<td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110106" target="_blank">BBa_K2110106</a></td> | <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110106" target="_blank">BBa_K2110106</a></td> | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
<td style="width:60%;text-align:left">PETase site-directed mutant S238V</td> | <td style="width:60%;text-align:left">PETase site-directed mutant S238V</td> | ||
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr><tr style="width:100%"> | </tr><tr style="width:100%"> | ||
<td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110107" target="_blank">BBa_K2110107</a></td> | <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110107" target="_blank">BBa_K2110107</a></td> | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
<td style="width:60%;text-align:left">PETase site-directed mutant L117F</td> | <td style="width:60%;text-align:left">PETase site-directed mutant L117F</td> | ||
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr><tr style="width:100%"> | </tr><tr style="width:100%"> | ||
<td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110108" target="_blank">BBa_K2110108</a></td> | <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110108" target="_blank">BBa_K2110108</a></td> | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
<td style="width:60%;text-align:left">PETase site-directed mutant I208F</td> | <td style="width:60%;text-align:left">PETase site-directed mutant I208F</td> | ||
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr> | </tr> | ||
<tr style="width:100%"> | <tr style="width:100%"> | ||
<td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110109" target="_blank">BBa_K2110109</a></td> | <td style="width:20%;text-align:left"><a href="http://parts.igem.org/Part:BBa_K2110109" target="_blank">BBa_K2110109</a></td> | ||
<td style="width:10%;text-align:left">Basic</td> | <td style="width:10%;text-align:left">Basic</td> | ||
| − | <td style="width:60%;text-align:left">PETase site-directed mutant | + | <td style="width:60%;text-align:left">PETase site-directed mutant S238F</td> |
| − | <td style="width:10%;text-align:left"> | + | <td style="width:10%;text-align:left">795</td> |
</tr> | </tr> | ||
<tr style="width:100%"> | <tr style="width:100%"> | ||
Revision as of 10:19, 17 October 2016
Parts
Parts For R-R System
| Biobrick | Type | Description | Length |
|---|---|---|---|
| BBa_K2110000 | Basic | CYC1 Terminator | 248 |
| BBa_K2110012 | Basic | PGK1 promoter | 781 |
| BBa_K2110017 | Basic | α-factor signal peptide | 267 |
| BBa_K2110001 | Basic | TPA inducible Leader sequence | 96 |
| BBa_K2110002 | Basic | TPA positive feedback regulatory gene | 807 |
| BBa_K2110003 | Basic | TPA transporting protein gene | 1380 |
| BBa_K2110004 | Basic | D-alanyl-D-alanine dipeptidase gene | 582 |
| BBa_K2110005 | Composite | TPA inducible PETase expression system | 2111 |
| BBa_K2110006 | Composite | TPA regulatory gene expression system | 1850 |
| BBa_K2110007 | Composite | TPA transporting protein gene expression system | 2425 |
| BBa_K2110008 | Composite | inclusion body induced cell lysis system | 665 |
Parts For Microbial Consortium
| Biobrick | Type | Description | Length |
|---|---|---|---|
| BBa_K2110009 | Basic | phage P22 holins | 327 |
| BBa_K2110010 | Basic | phage P22 auxiliary lysis factors | 438 |
| BBa_K2110011 | Basic | phage P22 endolysins | 441 |
| BBa_K2110013 | Basic | PETase enzyme after codon optimization for bacillus subtilis | 885 |
| BBa_K2110014 | Basic | MHETase enzyme after codon optimization for bacillus subtilis | 1848 |
| BBa_K2110015 | Basic | The key gene for accumulation of PHA in P.putida KT2440 | 978 |
| BBa_K2110016 | Basic | The key gene for utilization of ethylene glycolin P.putida KT2440 | 1326 |
Parts For Protein Modification
| Biobrick | Type | Description | Length |
|---|---|---|---|
| BBa_K2110800 | Basic | PETase | 795 |
| BBa_K2110100 | Basic | PETase site-directed mutant A240P | 795 |
| BBa_K2110101 | Basic | PETase site-directed mutant S207T | 795 |
| BBa_K2110102 | Basic | PETase site-directed mutant M154L | 795 |
| BBa_K2110103 | Basic | PETase site-directed mutant N205V | 795 |
| BBa_K2110104 | Basic | PETase site-directed mutant R90A | 795 |
| BBa_K2110105 | Basic | PETase site-directed mutant I208V | 795 |
| BBa_K2110106 | Basic | PETase site-directed mutant S238V | 795 |
| BBa_K2110107 | Basic | PETase site-directed mutant L117F | 795 |
| BBa_K2110108 | Basic | PETase site-directed mutant I208F | 795 |
| BBa_K2110109 | Basic | PETase site-directed mutant S238F | 795 |
| BBa_K2110200 | Composite | PETase expression system in Saccharomyces cerevisiae | 1961 |
