Difference between revisions of "Team:GDSYZX-United/Proof"
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<h2><strong>Expression level of protein HHL1</strong></h2> | <h2><strong>Expression level of protein HHL1</strong></h2> | ||
| − | <p><div class="text_font">After tranfection, we incubate the protoplasts at 2 groups of light intensities to induce the expression of hhl1.</div></p> | + | <p><div class="text_font" style="text-indent:0em;">After tranfection, we incubate the protoplasts at 2 groups of light intensities to induce the expression of hhl1.</div></p> |
| − | <p><div class="text_font">We use semi-quantitive RT-PCR to | + | <p><div class="text_font">We use semi-quantitive RT-PCR to detect the mRNA from gene hhl1 in protoplasts，the concentration of amplified cDNA of each group is measured by fluorescent quantitation. Data of the concentration of amplified products are used to calculate the relative expression level of mRNA.</div></p> |
<div align="center"> | <div align="center"> | ||
<img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/a/af/T--GDSYZX-United--proof1.jpg"/> | <img alt="image" class="img-responsive" src="https://static.igem.org/mediawiki/2016/a/af/T--GDSYZX-United--proof1.jpg"/> | ||
</div> | </div> | ||
| − | <table class= "table"> | + | <table class="table" style="text-align:center"> |
<thead> | <thead> | ||
<th></th> | <th></th> | ||
| − | <th colspan="2" | + | <th colspan="2" style="text-align:center">Growth light</th> |
| − | <th colspan="2" | + | <th colspan="2" style="text-align:center">High light</th> |
</thead> | </thead> | ||
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| − | <p><div class="text_font">This graph shows the relative expression level of mRNA of gene hhl1 expressed by 4 different parts | + | <p><div class="text_font">This graph shows the relative expression level of mRNA of gene hhl1 expressed by 4 different parts under growth light(100lux) and high light(1200lux).</div></p> |
<p><div class="text_font">pCop1,pPif1,pPhyB do not respond to growth light, the expression level of mRNA is similar to that of pHhl1.However, under high light,three photosensitive promoters all respond specifically and the expression level of mRNA is obviously higher than that in pHhl1.(The transcription level of pCop1,pPif1 and pPhyB under highlight are 10.1,8.9 and 15.6 times of the mRNA level under growth light,respectively.),suggesting that our parts can help produce more protein HHL1 to protect PSⅡ under high light.</div></p> | <p><div class="text_font">pCop1,pPif1,pPhyB do not respond to growth light, the expression level of mRNA is similar to that of pHhl1.However, under high light,three photosensitive promoters all respond specifically and the expression level of mRNA is obviously higher than that in pHhl1.(The transcription level of pCop1,pPif1 and pPhyB under highlight are 10.1,8.9 and 15.6 times of the mRNA level under growth light,respectively.),suggesting that our parts can help produce more protein HHL1 to protect PSⅡ under high light.</div></p> | ||
<p><div class="text_font">(the relative expression level of mRNA of pHhl1 under high light doesn’t change much,but after translation the expression level of protein HHL1 is increased compare to pHhl1 under growth light).</div></p> | <p><div class="text_font">(the relative expression level of mRNA of pHhl1 under high light doesn’t change much,but after translation the expression level of protein HHL1 is increased compare to pHhl1 under growth light).</div></p> | ||
Latest revision as of 14:55, 17 October 2016
Proof of concept
Expression level of protein HHL1
After tranfection, we incubate the protoplasts at 2 groups of light intensities to induce the expression of hhl1.
We use semi-quantitive RT-PCR to detect the mRNA from gene hhl1 in protoplasts，the concentration of amplified cDNA of each group is measured by fluorescent quantitation. Data of the concentration of amplified products are used to calculate the relative expression level of mRNA.
| Growth light | High light | |||
|---|---|---|---|---|
| REL | SE | REL | SE | |
| Phhl1 | 1 | 0.05 | 1.1 | 0.07 |
| Pcop1 | 1 | 0.03 | 10.1 | 1.26 |
| Ppif1 | 1 | 0.06 | 8.9 | 1.01 |
| PphyB | 1 | 0.09 | 15.6 | 1.36 |
This graph shows the relative expression level of mRNA of gene hhl1 expressed by 4 different parts under growth light(100lux) and high light(1200lux).
pCop1,pPif1,pPhyB do not respond to growth light, the expression level of mRNA is similar to that of pHhl1.However, under high light,three photosensitive promoters all respond specifically and the expression level of mRNA is obviously higher than that in pHhl1.(The transcription level of pCop1,pPif1 and pPhyB under highlight are 10.1,8.9 and 15.6 times of the mRNA level under growth light,respectively.),suggesting that our parts can help produce more protein HHL1 to protect PSⅡ under high light.
(the relative expression level of mRNA of pHhl1 under high light doesn’t change much,but after translation the expression level of protein HHL1 is increased compare to pHhl1 under growth light).
