Difference between revisions of "Team:Harvard BioDesign/Composite Part"

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<header>
 
<header>
 
<h2><a href="#">Composite Parts</a></h2>
 
<h2><a href="#">Composite Parts</a></h2>
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<p>The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.</p>
+
 +
 
 +
<p>The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.</p>
 
<p>Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.</p>
 
<p>Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.</p>
 
<section>
 
<section>
 
<p>Overall, there are 8 composite parts, which consist of the following:</p>
 
<p>Overall, there are 8 composite parts, which consist of the following:</p>
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<ul>BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon</ul>
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<b>
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<ul>BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon</ul>
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<p>BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon</p>
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<ul>BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon</ul>
+
<p>BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon</p>
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<ul>BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon</ul>
+
<p>BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon</p>
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<ul>BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon</ul>
+
<p>BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon</p>
−
<ul>BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon</ul>
+
<p>BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon</p>
−
<ul>BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon</ul>
+
<p>BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon</p>
−
<ul>BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon</ul>
+
<p>BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon</p>
 +
<p>BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon</p>
 +
</b>
 
</section>
 
</section>
 +
 +
 
</article>
 
</article>
  

Revision as of 02:54, 18 October 2016

Harvard BioDesign 2016

Composite Parts

The composite parts, in general, involve a combination of the T7 promoter, RBS 34, PETase, His-tag, and a stop codon.

Additional features such as sfGFP and secretion tags were used in order to optimize the production, detection, and secretion of PETase.

Overall, there are 8 composite parts, which consist of the following:

BBa_K201000: T7 Promoter + PETase + His-Tag + Stop Codon

BBa_K201001: T7 Promoter + ompT + PETase + His-Tag + Stop Codon

BBa_K201002: T7 Promoter + pelB + PETase + His-Tag + Stop Codon

BBa_K201003: T7 Promoter + YebF + PETase + His-Tag + Stop Codon

BBa_K201004: T7 Promoter + sfGFP + PETase + His-Tag + Stop Codon

BBa_K201005: T7 Promoter + sfGFP + ompT + PETase + His-Tag + Stop Codon

BBa_K201007: T7 Promoter + sfGFP + pelB + PETase + His-Tag + Stop Codon

BBa_K201008: T7 Promoter + sfGFP + YebF + PETase + His-Tag + Stop Codon