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                            <h1>Team Tianjin</h1>
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                                <div class="separator line-separator">∎</div>
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                            <h5>"Plastic tastes good." &nbsp; --- &nbsp; <i>Yeast</i></h5>
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                                <h2>A brief description</h2>
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                                <p class="TeamTianjin-text-main">
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                                    This March our team paid much attention to an article <i class="ref-title">A bacterium that degrades and assimilates poly(ethylene terephthalate)</i><a class="btn popover-info ref-title-link" data-toggle="popover" data-placement="top" title="Reference" data-content="Yoshida, Shosuke, et al. 'A bacterium that degrades and assimilates poly (ethylene terephthalate).' Science 351.6278 (2016): 1196-1199.">1</a> published in Science in the same month. A new kind of bacteria that can decompose PET was found and studied in detail. We plan to express its unique genes in some commonly used mode organisms such as yeasts and E.colis to enhance its activities of decomposition significantly since they are relatively low at present.
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                                     We have synthesized the gene sequences of the PETase and MHETase based on the supplementary materials of the original paper after several months’ literature reviewing. And we began several preliminary experiments to figure out if those exogenous genes could be well expressed in the host cells. We decide to enhance the activities of these two enzymes via surface display, protein scaffold and fusion expression. Another way to enhance the rate of reactions is to put the first (hydrolysis of PET) and the second step (hydrolysis of MHET) together by cascade catalysis.
 
                                     We have synthesized the gene sequences of the PETase and MHETase based on the supplementary materials of the original paper after several months’ literature reviewing. And we began several preliminary experiments to figure out if those exogenous genes could be well expressed in the host cells. We decide to enhance the activities of these two enzymes via surface display, protein scaffold and fusion expression. Another way to enhance the rate of reactions is to put the first (hydrolysis of PET) and the second step (hydrolysis of MHET) together by cascade catalysis.
 
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                                     We hope to construct a system that can efficiently express and secrete (or display) these two enzymes. The system will be able to hydrolyze PET with a much higher rate than the Ideonella sakaiensis reported in the thesis.  
 
                                     We hope to construct a system that can efficiently express and secrete (or display) these two enzymes. The system will be able to hydrolyze PET with a much higher rate than the Ideonella sakaiensis reported in the thesis.  
 
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  <b>Notice:</b> This page is currently under construction. Contents in this page are temporaory and will be modified several times before the final release. &nbsp;&nbsp;&nbsp; &#8212; 2016 iGEM Team Tianjin
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Revision as of 01:30, 9 July 2016

TEAM TIANJIN

LOGO


TeamTianjin Back Ground

Team Tianjin

∎
"Plastic tastes good."   ---   Yeast

A brief description

This March our team paid much attention to an article A bacterium that degrades and assimilates poly(ethylene terephthalate)1 published in Science in the same month. A new kind of bacteria that can decompose PET was found and studied in detail. We plan to express its unique genes in some commonly used mode organisms such as yeasts and E.colis to enhance its activities of decomposition significantly since they are relatively low at present.

Current situation

We have synthesized the gene sequences of the PETase and MHETase based on the supplementary materials of the original paper after several months’ literature reviewing. And we began several preliminary experiments to figure out if those exogenous genes could be well expressed in the host cells. We decide to enhance the activities of these two enzymes via surface display, protein scaffold and fusion expression. Another way to enhance the rate of reactions is to put the first (hydrolysis of PET) and the second step (hydrolysis of MHET) together by cascade catalysis.

Vision

We hope to construct a system that can efficiently express and secrete (or display) these two enzymes. The system will be able to hydrolyze PET with a much higher rate than the Ideonella sakaiensis reported in the thesis.

info_outline
Notice: This page is currently under construction. Contents in this page are temporaory and will be modified several times before the final release.     — 2016 iGEM Team Tianjin