Difference between revisions of "Team:BIT-China/Collaborations"

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                 <!--吉林大学-->
 
                 <!--吉林大学-->
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                 <div id="jilin_china" class="block-title col-sm-12">Jilin_China</div>
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                 <div id="jilin_china" class="block-title col-sm-12">
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                    Collaboration with Jilin-China
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                    <div style="font-size: 0.7em"> [debugging a construct]</div>
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                </div>
 
                 <div class="block-content">
 
                 <div class="block-content">
 
                     <div class="block-content-item">
 
                     <div class="block-content-item">
  
 
                         <div class="block-content-item-block">
 
                         <div class="block-content-item-block">
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                            <div class="block-header-margin1">
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                                <i class="fa fa-hand-o-right" aria-hidden="true"></i>
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                                <span>◎ BIT-China for Jilin-China</span>
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                            </div>
 
                             <div>
 
                             <div>
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                                 This year, Jilin_China mainly helps us to improve and construct a model of
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                                <a href="##" style="color: blue;text-decoration: underline">[放对方队伍的链接]</a>
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                                 substance changes about the experiment circuit (Fig. 1). We provide them
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                            </div>
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                                the conditions and equations of each stage of chemical reaction and
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                            <div class="block-paragraph">
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                                they are responsible for
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                                 This year, team Jilin_China aimed to propose a new way to cure the solid tumor by expressing the TAT-Apoptin. The chassis they chose is Bifidobacteria, which can target the anaerobic region of the solid tumors since the microorganism is also anaerobic.
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                                the realization of the equations and the whole regulating process.
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                            </div>
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                            <div class="block-paragraph">
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                                 However, the functional device should firstly be transformed into E.coli to operate the methylation process in case that the devices were digested by the restriction enzymes inside the Bifidobacterium longum.
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                            </div>
 +
                            <div class="block-paragraph">
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                                Our team BIT-China helped them realize the expression of TAT-Apoptin in E.coli. After transformation, the cells were digested and centrifuged to collect the expression products of the bacteria, and the proteins of the samples separated by SDS-PAGE, and the results were as follows.
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                                <br>(这一段要替换)
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                            </div>
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                            <div class="block-header-margin1">
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                                <i class="fa fa-hand-o-right" aria-hidden="true"></i>
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                                <span>◎ Jilin-China for BIT-China</span>
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                            </div>
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                            <div class="block-paragraph">
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                                This year, Jilin_China mainly helped us improve our model.
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                            </div>
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                            <div class="block-paragraph">
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                                After constructing the primary model, we met some difficulties in proving the accuracy of these equations, as well as simulating these equations by software. Some members of Jilin_China majored in physics, math, computer science and chemistry, they were familiar with solving these problems and kindly provided us with much useful advice.
 +
                            </div>
 +
                            <div class="block-paragraph">
 +
                                We provide them the conditions and equations of each stage of chemical reaction. They add some chemical reaction to improve our modeling and get the analytic solutions of the linearized equations.
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/c/c2/T--BIT-China--Team--Collaborations--fig1.png"
 
                                 <img src="https://static.igem.org/mediawiki/2016/c/c2/T--BIT-China--Team--Collaborations--fig1.png"
 
                                     alt="fig1"  style="height: 80px;" class="center-block">
 
                                     alt="fig1"  style="height: 80px;" class="center-block">
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                                <div class="pic_info">
 +
                                    Fig. 1 The experiment circuit
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                                </div>
 
                             </div>
 
                             </div>
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                             <div>
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                             <div style="padding: 0 30%;">
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                                 BIT-China construct the modeling equations preliminarily.
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                                 <table class="table table-bordered" style="background: whitesmoke">
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                                The difficulty which we meet is how to identify the equations are right,
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                                    <thead>
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                                how can we realize equations by modeling software and how to draw an image
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                                    <tr>
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                                to reflect the whole process of the change of the substances. Because some
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                                        <th>Variable1</th>
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                                 members of Jilin_China, they are majoring in physics, math, computer science
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                                        <th>Variable2</th>
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                                 and chemistry, they have much experience in differential equations, application
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                                        <th>Description</th>
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                                 of MATLAB and designing of computer programs. With these resources,
+
                                    </tr>
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                                they have helped us solve our problems successfully.
+
                                    </thead>
 +
                                    <tbody>
 +
                                    <tr>
 +
                                        <td>D<span class="small">t</span></td>
 +
                                        <td>C<span class="small">3</span></td>
 +
                                        <td>Number of plasmids</td>
 +
                                    </tr>
 +
                                    </tbody>
 +
                                 </table>
 +
                                 <div class="pic_info">
 +
                                    Table 1. The description of variables used in differential reaction equations
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                                 </div>
 
                             </div>
 
                             </div>
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                             <div>
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                             <div class="block-paragraph">
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                                <img src="https://static.igem.org/mediawiki/2016/a/a0/T--BIT-China--Team--Collaborations--table1.png" alt="table1" style="width: 800px;"class="center-block">
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                                 Based on the equations we provided, they added some modification.
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                            </div>
+
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                            <div>
+
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                                 Firstly, basing on the equations we provided they initial modify some of the equations.(Fig. 2).
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                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/d/d8/T--BIT-China--Team--Collaborations--fig2.png
 
                                 <img src="https://static.igem.org/mediawiki/2016/d/d8/T--BIT-China--Team--Collaborations--fig2.png
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                                 " alt="fig2" style="height:60px;"class="center-block">
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                                 " alt="fig2" style="height: 50px;" class="center-block">
 +
                                <div class="pic_info">
 +
                                    Fig. 2 The equations improved by JLU
 +
                                </div>
 
                             </div>
 
                             </div>
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                             <div>
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                             <div class="block-paragraph">
−
                                 Secondly, after several discussions with us, JLU understood the background of
+
                                 After discussion, we found it’s hard to measure some parameters. They suggested us to simplify the equations, making it more close to the linear relationship.
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                                our modeling part and the problems they were about to settle. Because of some
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                            </div>
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                                parameters in the equations is hard to measure, they reduce some unnecessary
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                            <div class="block-paragraph">
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                                equations and change the number of the variables, which make the process through
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                                 The picture has two parts, the one part is about the equations replaced by other symbols (Fig. 4), another part is about the linear equations to avoid measuring the parameter (Fig. 5).
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                                linear equations. The first picture is about the equations to describe the chemical
+
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                                reaction processes between the enzyme and substrate made by BIT-China (Fig. 3).
+
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                                 The second picture has two parts, the one part is about the equations replace by
+
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                                other symbols (Fig. 4), another part is about the linear equations to avoid
+
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                                measuring the parameter (Fig. 5).
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                                So we can use our experiment results to fit the equations by some modeling software.
+
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
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                                 <img src="https://static.igem.org/mediawiki/2016/4/48/T--BIT-China--Team--Collaborations--fig3.png
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                                 <!--<img src="https://static.igem.org/mediawiki/2016/4/48/T&#45;&#45;BIT-China&#45;&#45;Team&#45;&#45;Collaborations&#45;&#45;fig3.png-->
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                                 " alt="fig3" style="width: 700px;" class="center-block">
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                                 <!--" alt="fig3" style="width: 80%;">-->
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
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                                 <img src="https://static.igem.org/mediawiki/2016/d/d4/T--BIT-China--Team--Collaborations--fig4.png" alt="fig4"style="width:600px" class="center-block">
+
                                 <img src="https://static.igem.org/mediawiki/2016/d/d4/T--BIT-China--Team--Collaborations--fig4.png"
 +
                                    alt="fig4" style="width: 500px;" class="center-block">
 +
                                <div class="pic_info">
 +
                                    Fig. 4 Linear equations made by JLU
 +
                                </div>
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/1/1e/T--BIT-China--Team--Collaborations--fig5.png"
 
                                 <img src="https://static.igem.org/mediawiki/2016/1/1e/T--BIT-China--Team--Collaborations--fig5.png"
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                                     alt="fig5" style="width: 80%;"class="center-block">
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                                     alt="fig5" style="width: 500px;" class="center-block">
 +
                                <div class="pic_info">
 +
                                    Fig. 5 Equations replace by other symbols
 +
                                </div>
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
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                 <!--兰州大学-->
 
                 <!--兰州大学-->
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                 <div id="lanzhou" class="block-title col-sm-12">Lanzhou</div>
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                 <div id="lanzhou" class="block-title col-sm-12">
 +
                    Collaboration with Lanzhou
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                    <div style="font-size: 0.7em">[confirming the mercury concentration]</div>
 +
                </div>
 
                 <div class="block-content">
 
                 <div class="block-content">
 
                     <div class="block-content-item">
 
                     <div class="block-content-item">
  
 
                         <div class="block-content-item-block">
 
                         <div class="block-content-item-block">
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                             <div>
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                             <div class="block-header-margin1">
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                                 On September 18th, 2016, we met and exchanged ideas with members of
+
                                 <i class="fa fa-hand-o-right" aria-hidden="true"></i>
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                                teams from Lanzhou by the Beijing Institute of Technology. After the
+
                                 <span>◎ BIT-China for Lanzhou</span>
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                                meeting, we confirmed our collaboration relationship.
+
−
                                 <b>
+
−
                                    To further extend
+
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                                    our system, we asked them
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                                    for helping us constructing the new BioBrick of our gene circuits.
+
−
                                </b>
+
 
                             </div>
 
                             </div>
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                             <div>
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                             <div><a href="##">[放对方队伍的链接]</a></div>
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                                They helped us finish the "another inhibitor" system
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                            <div class="block-paragraph">
−
                                (<span class="italic">Arac</span>+pBAD+B0034+TetR+B0015+P<sub>Tet</sub>+B0034+<span class="italic">rfp</span>+B0015)
+
                                This year, team Lanzhou aimed to employ the gut flora to treat the mercury pollution inside fish body. To transform the gut flora, they planned to feed the fish with genetic engineered bacteria.
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/7/75/T--BIT-China--Team--Collaborations--fig6.png
 
                                 <img src="https://static.igem.org/mediawiki/2016/7/75/T--BIT-China--Team--Collaborations--fig6.png
−
                                 " alt="fig6" style="height: 100px;"class="center-block">>
+
                                 " alt="fig6" style="height: 100px;" class="center-block">
 +
                                <div class="pic_info">
 +
 
 +
                                </div>
 
                             </div>
 
                             </div>
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                             <div>In this system, pBAD is one of the inducible promoter
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                             <div class="block-paragraph">
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                                 (fromBBa_K808000) which can be induced by arabinose. B0034 and
+
                                 Above is the gene circuit they designed, INP (Ice Nucleation Protein) is an extrinsic membrane protein of E.coli and acts as an anchor. GFP acts as a reporter gene while metal catcher can bind heavy-metal ions.
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                                 B0032 are two kinds of strong ribosome binding sites (RBS) and the
+
                            </div>
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                                strength of B0032 is only 33.96% of
+
                            <div class="block-paragraph">
−
                                B0034. P<sub>Tet</sub> promoter is constitutively ON and repressed by TetR repressor.
+
                                 We helped them designed and operated the pre-experiment to identify the maximum concentration of mercury ions which can keep the fish alive. From literature research, we chose the following concentration: 0.3mg/L, 0.2mg/L, 0.15mg/L, 0.1mg/L, 0.05mg/L, 0.025mg/L.
 +
                            </div>
 +
                            <div class="block-paragraph">
 +
                                Then we raised 10 fishes and measured the number at the same time in fifteen days.
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
−
                                 The "inhibitor" system is very important for our project, we use the
+
                                 <img src="https://static.igem.org/mediawiki/2016/e/eb/T--BIT-China--Team--Collaborations--fig6p.png"
−
                                concentration of inhibitor proteins as a signal indicating the plasmid
+
                                    alt="fig6p" style="height: 300px;" class="center-block">
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                                 numbers, and it can trigger the downstream reaction by activating the
+
                                 <div class="pic_info">
−
                                killer gene while the plasmid concentration reduce the threshold. So,
+
 
−
                                 in this circuit, we use the red
+
                                 </div>
−
                                fluorescent protein (RFP) to simulate the threshold of the P<sub>Tet</sub> promoter.
+
 
                             </div>
 
                             </div>
 +
                            <div class="block-paragraph">
 +
                                At last, we found that the concentration of mercury ions should be maintained at 0.1 milligrams per liter. When the concentration is higher than this value, the fish will die. When the concentration is lower than this value, the difference will not be obvious.
 +
                            </div>
 +
 +
                            <div class="block-header-margin1">
 +
                                <i class="fa fa-hand-o-right" aria-hidden="true"></i>
 +
                                <span>◎ Lanzhou for BIT-China</span>
 +
                            </div>
 +
                            <div class="block-paragraph">
 +
                                On September 18th, 2016, we met and exchanged ideas with members of teams from Lanzhou by the Beijing Institute of Technology. After the meeting, we confirmed our collaboration relationship. To further extend our system, we asked them for helping us constructing one of our gene circuits.
 +
 +
                            </div>
 +
                            <div class="block-paragraph">
 +
                                They constructed one of our testing circuits.
 +
                            </div>
 +
                            <div>
 +
                                <img src="" alt="图片标红了，需要修改">
 +
                            </div>
 +
                            <div class="block-paragraph">
 +
                                The "inhibitor" system is very important for our project, we use the concentration of inhibitor proteins as a signal indicating the plasmid numbers, and it can trigger the downstream reaction by activating the killer gene while the plasmid concentration reduce the threshold.
 +
                            </div>
 +
 
                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
Line 215: Line 280:
  
 
                 <!--CIEI-BJ-->
 
                 <!--CIEI-BJ-->
−
                 <div id="ciei_bj" class="block-title col-sm-12">CIEI-BJ</div>
+
                 <div id="ciei_bj" class="block-title col-sm-12">
 +
                    3. Collaboration with CIEI-BJ
 +
                    <div style="font-size: 0.7em">
 +
                        [mentoring a new high school team]
 +
                    </div>
 +
                </div>
 
                 <div class="block-content">
 
                 <div class="block-content">
 
                     <div class="block-content-item">
 
                     <div class="block-content-item">
  
 
                         <div class="block-content-item-block">
 
                         <div class="block-content-item-block">
 +
                            <div class="block-header-margin1">
 +
                                <i class="fa fa-hand-o-right" aria-hidden="true"></i>
 +
                                <span>◎ BIT-China for CIEI-BJ</span>
 +
                            </div>
 
                             <div>
 
                             <div>
−
                                 CIEI-BJ is a high school team and it’s their first time to participate in
+
                                <a href="https://2016.igem.org/Team:CIEI-BJ/Collaborations" style="color: blue;text-decoration: underline">
−
                                iGEM competition this year. In the middle of July, team CIEI-BJ came to
+
                                    https://2016.igem.org/Team:CIEI-BJ/Collaborations
−
                                visit Beijing Institute
+
                                </a>
−
                                of Technology and communicated with our team BIT-China.
+
                            </div>
 +
                            <div class="block-paragraph">
 +
                                 CIEI-BJ is a high school team and it’s their first time to participate in iGEM competition this year. In the middle of July, team CIEI-BJ came to visit Beijing Institute of Technology and communicated with our team BIT-China.
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/c/ca/T--BIT-China--Team--Collaborations--fig7.png
 
                                 <img src="https://static.igem.org/mediawiki/2016/c/ca/T--BIT-China--Team--Collaborations--fig7.png
−
                                 " alt="fig7" style="width: 80%;"class="center-block">>
+
                                 " alt="fig7" style="height: 400px;" class="center-block">
 
                             </div>
 
                             </div>
−
                             <div>
+
                             <div class="block-paragraph">
−
                                 As an undergraduate team, we shared the core concept of our project this year
+
                                 As an undergraduate team, we shared the core concept of our project this year and explained a little bit about how we have exchanged ideas among the team members and how to assess the feasibility of our design as a reference for high school students.
−
                                and explained a little bit about how we have exchanged ideas among the
+
−
                                team members and how to assess
+
−
                                the feasibility of our design as a reference for high school students.
+
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/c/c2/T--BIT-China--Team--Collaborations--fig8.png
 
                                 <img src="https://static.igem.org/mediawiki/2016/c/c2/T--BIT-China--Team--Collaborations--fig8.png
−
                                 " alt="fig8"style="width: 80%;"class="center-block">>
+
                                 " alt="fig8"style="width: 400px;" class="center-block">
 
                             </div>
 
                             </div>
−
                             <div>
+
                             <div class="block-paragraph">
−
                                 Later, team members in high school also shared their fascinating ideas.
+
                                 Later, team members in high school also shared their fascinating ideas. However, some plans are not that practical due to the objective reasons. A lot of questioned were raised by members of BIT-China to help shape the project of team CIEI-BJ this year. The considerations refer to the practical meaning and the possibility of conduct for an appropriate project. What’s more, some suggestions are given to optimize the details like designing an experiment to prove a concept.
−
                                However, some plans are not that practical due to the objective reasons.
+
−
                                A lot of questioned were raised by members of BIT-China to help
+
−
                                shape the project of team CIEI-BJ this year. The considerations refer to
+
−
                                the practical meaning and the possibility of conduct for an appropriate
+
−
                                project. What’s more, some suggestions are given to
+
−
                                optimize the details like designing an experiment to prove a concept.
+
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/6/67/T--BIT-China--Team--Collaborations--fig9.png"
 
                                 <img src="https://static.igem.org/mediawiki/2016/6/67/T--BIT-China--Team--Collaborations--fig9.png"
−
                                     alt="fig9" style="width: 80%;"class="center-block">>
+
                                     alt="fig9" style="width: 400px;" class="center-block">
 
                             </div>
 
                             </div>
−
                             <div>
+
                             <div class="block-paragraph">
−
                                 After the discussion, we showed high school students around our lab and explained
+
                                 After the discussion, we showed high school students around our lab and explained the brief function of some experimental instruments. Besides, we introduced them to the previous projects of our team with the aid of posters preserved.
−
                                the brief function of some experimental instruments. Besides, we introduced them to the
+
−
                                previous projects of our team with the aid of posters preserved.
+
 
                             </div>
 
                             </div>
−
                             <div>
+
                             <div class="block-paragraph">
−
                                 We help them design the basic gene circuit. In the next few months, the guidance
+
                                 After they designed the basic gene circuit, we gave some advice on how to improve it. In the next few months, the guidance of BIT-China to CIEI-BJ are not only limited to the project itself, but also extended to the details about the competition procedures. We have been in touch with CIEI-BJ through the Internet and give advice on many aspects, like how to present our iGEM program to potential sponsors and how to achieve the requirements for deliverables.
−
                                of BIT-China to CIEI-BJ are not only limited to the project itself, but also extended
+
−
                                to the details about the competition procedures. We have been in touch with
+
−
                                CIEI-BJ through the Internet and give advice on many aspects, like how to
+
−
                                present our iGEM program to potential sponsors
+
−
                                and how to achieve the requirements for deliverables.
+
 
                             </div>
 
                             </div>
−
                             <div>
+
                             <div class="block-paragraph">
−
                                 In September 17th, 2016, some members of BIT-China are invited to share their
+
                                 In September 17th, 2016, some members of BIT-China are invited to share their experience on how to design the wiki page. A few examples were given to explain the good elements in introducing the project in a simple way. What should be put on the wiki were also discussed during this meeting. Considering the web fluency and in order to design a clear page, notes are listed for high school members to pay attention when they are working on their wiki.
−
                                experience on how to design the wiki page. A few examples were given to
+
−
                                explain the good elements in introducing the project in a simple way.
+
−
                                What should be put on the wiki were also discussed during this meeting.
+
−
                                Considering the web fluency and in order to design a clear page, notes are
+
−
                                listed for high school
+
−
                                members to pay attention when they are working on their wiki.
+
 
                             </div>
 
                             </div>
 
                             <div>
 
                             <div>
 
                                 <img src="https://static.igem.org/mediawiki/2016/a/a8/T--BIT-China--Team--Collaborations--fig10.png
 
                                 <img src="https://static.igem.org/mediawiki/2016/a/a8/T--BIT-China--Team--Collaborations--fig10.png
−
                                 " alt="fig10" style="width: 80%;"class="center-block">>
+
                                 " alt="fig10" style="width: 400px;" class="center-block">
 
                             </div>
 
                             </div>
−
                             <div>
+
                             <div class="block-paragraph">
−
                                 In the process of mentoring a high school team, we also benefited a lot.
+
                                 In the process of mentoring a high school team, we also benefited a lot. Not only did we engage in the competition, but also we had a better understanding of the community connected by iGEM.
−
                                Not only did we engage in the competition, but also we
+
−
                                had a better understanding of the community connected by iGEM.
+
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>

Revision as of 00:27, 19 October 2016

backtop
title

Collaboration with Jilin-China
[debugging a construct]
◎ BIT-China for Jilin-China
This year, team Jilin_China aimed to propose a new way to cure the solid tumor by expressing the TAT-Apoptin. The chassis they chose is Bifidobacteria, which can target the anaerobic region of the solid tumors since the microorganism is also anaerobic.
However, the functional device should firstly be transformed into E.coli to operate the methylation process in case that the devices were digested by the restriction enzymes inside the Bifidobacterium longum.
Our team BIT-China helped them realize the expression of TAT-Apoptin in E.coli. After transformation, the cells were digested and centrifuged to collect the expression products of the bacteria, and the proteins of the samples separated by SDS-PAGE, and the results were as follows.
(这一段要替换)
◎ Jilin-China for BIT-China
This year, Jilin_China mainly helped us improve our model.
After constructing the primary model, we met some difficulties in proving the accuracy of these equations, as well as simulating these equations by software. Some members of Jilin_China majored in physics, math, computer science and chemistry, they were familiar with solving these problems and kindly provided us with much useful advice.
We provide them the conditions and equations of each stage of chemical reaction. They add some chemical reaction to improve our modeling and get the analytic solutions of the linearized equations.
fig1
Fig. 1 The experiment circuit
Variable1 Variable2 Description
Dt C3 Number of plasmids
Table 1. The description of variables used in differential reaction equations
Based on the equations we provided, they added some modification.
fig2
Fig. 2 The equations improved by JLU
After discussion, we found it’s hard to measure some parameters. They suggested us to simplify the equations, making it more close to the linear relationship.
The picture has two parts, the one part is about the equations replaced by other symbols (Fig. 4), another part is about the linear equations to avoid measuring the parameter (Fig. 5).
fig4
Fig. 4 Linear equations made by JLU
fig5
Fig. 5 Equations replace by other symbols
Collaboration with Lanzhou
[confirming the mercury concentration]
◎ BIT-China for Lanzhou
This year, team Lanzhou aimed to employ the gut flora to treat the mercury pollution inside fish body. To transform the gut flora, they planned to feed the fish with genetic engineered bacteria.
fig6
Above is the gene circuit they designed, INP (Ice Nucleation Protein) is an extrinsic membrane protein of E.coli and acts as an anchor. GFP acts as a reporter gene while metal catcher can bind heavy-metal ions.
We helped them designed and operated the pre-experiment to identify the maximum concentration of mercury ions which can keep the fish alive. From literature research, we chose the following concentration: 0.3mg/L, 0.2mg/L, 0.15mg/L, 0.1mg/L, 0.05mg/L, 0.025mg/L.
Then we raised 10 fishes and measured the number at the same time in fifteen days.
fig6p
At last, we found that the concentration of mercury ions should be maintained at 0.1 milligrams per liter. When the concentration is higher than this value, the fish will die. When the concentration is lower than this value, the difference will not be obvious.
◎ Lanzhou for BIT-China
On September 18th, 2016, we met and exchanged ideas with members of teams from Lanzhou by the Beijing Institute of Technology. After the meeting, we confirmed our collaboration relationship. To further extend our system, we asked them for helping us constructing one of our gene circuits.
They constructed one of our testing circuits.
图片标红了，需要修改
The "inhibitor" system is very important for our project, we use the concentration of inhibitor proteins as a signal indicating the plasmid numbers, and it can trigger the downstream reaction by activating the killer gene while the plasmid concentration reduce the threshold.
3. Collaboration with CIEI-BJ
[mentoring a new high school team]
◎ BIT-China for CIEI-BJ
CIEI-BJ is a high school team and it’s their first time to participate in iGEM competition this year. In the middle of July, team CIEI-BJ came to visit Beijing Institute of Technology and communicated with our team BIT-China.
fig7
As an undergraduate team, we shared the core concept of our project this year and explained a little bit about how we have exchanged ideas among the team members and how to assess the feasibility of our design as a reference for high school students.
fig8
Later, team members in high school also shared their fascinating ideas. However, some plans are not that practical due to the objective reasons. A lot of questioned were raised by members of BIT-China to help shape the project of team CIEI-BJ this year. The considerations refer to the practical meaning and the possibility of conduct for an appropriate project. What’s more, some suggestions are given to optimize the details like designing an experiment to prove a concept.
fig9
After the discussion, we showed high school students around our lab and explained the brief function of some experimental instruments. Besides, we introduced them to the previous projects of our team with the aid of posters preserved.
After they designed the basic gene circuit, we gave some advice on how to improve it. In the next few months, the guidance of BIT-China to CIEI-BJ are not only limited to the project itself, but also extended to the details about the competition procedures. We have been in touch with CIEI-BJ through the Internet and give advice on many aspects, like how to present our iGEM program to potential sponsors and how to achieve the requirements for deliverables.
In September 17th, 2016, some members of BIT-China are invited to share their experience on how to design the wiki page. A few examples were given to explain the good elements in introducing the project in a simple way. What should be put on the wiki were also discussed during this meeting. Considering the web fluency and in order to design a clear page, notes are listed for high school members to pay attention when they are working on their wiki.
fig10
In the process of mentoring a high school team, we also benefited a lot. Not only did we engage in the competition, but also we had a better understanding of the community connected by iGEM.

P-SLACKiller

· Work or Die
· Monitor Plasmid Numbers
· Kill the Slacker
· Maintain the Better Biosystem

Useful Links

Sponsors

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Get in touch

Email: iGEM_BIT_China@163.com
Location: No.5 Zhongguancun South Street

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