Difference between revisions of "GDSYZX-United/NOTEBOOK/day note"
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<meta name="viewport" content="width=device-width, initial-scale=1.0"> | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
| − | + | <link href="https://2016.igem.org/Template:GDSYZX-United/CSS/bootstrap_min14ed?action=raw&ctype=text/css&v=3.3.6" rel="stylesheet"> | |
<link href="https://2016.igem.org/Template:GDSYZX-United/CSS/font-awesome_min93e3?action=raw&ctype=text/css&v=4.4.0" rel="stylesheet"> | <link href="https://2016.igem.org/Template:GDSYZX-United/CSS/font-awesome_min93e3?action=raw&ctype=text/css&v=4.4.0" rel="stylesheet"> | ||
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background: -moz-linear-gradient(right,rgba(195,243,219,1),rgba(228,249,234,0.1)); /* Firefox 3.6 - 15*/ | background: -moz-linear-gradient(right,rgba(195,243,219,1),rgba(228,249,234,0.1)); /* Firefox 3.6 - 15*/ | ||
background: linear-gradient(to right, rgba(195,243,219,1), rgba(228,249,234,0.1)); /* 标准的语法 */ | background: linear-gradient(to right, rgba(195,243,219,1), rgba(228,249,234,0.1)); /* 标准的语法 */ | ||
| − | padding-top: | + | padding-top:10px; |
} | } | ||
nav {padding-top:10px;} | nav {padding-top:10px;} | ||
| + | .text_font { | ||
| + | line-height:120%; | ||
| + | font-size:130% | ||
| + | } | ||
</style> | </style> | ||
</head> | </head> | ||
| Line 35: | Line 39: | ||
<ul class="nav navbar-nav navbar-right"> | <ul class="nav navbar-nav navbar-right"> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United"> HOME </a> | + | <a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United">HOME</a> |
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <span class="caret"></span></a> | + | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown">TEAM<span class="caret"></span></a> |
<ul role="menu" class="dropdown-menu"> | <ul role="menu" class="dropdown-menu"> | ||
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/GDSYZX-United/TEAM/team_members">Team members</a></li> |
| + | <li><a href="https://2016.igem.org/Team:GDSYZX-United/Collaborations">Collarboration</a></li> | ||
| + | <li><a href="https://2016.igem.org/Team:GDSYZX-United/Attributions">Attribution</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <span class="caret"></span></a> | + | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown">PROJECT<span class="caret"></span></a> |
<ul role="menu" class="dropdown-menu"> | <ul role="menu" class="dropdown-menu"> | ||
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/Team:GDSYZX-United/Description">Project description</a></li> |
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/extract _dna">Extract DNA in arabidopsis thaliana</a></li> |
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/enzyme_cleavage">Enzyme cleavage</a></li> |
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/preparation_arabidopsis_protoplast">Preparation of the Arabidopsis protoplast</a></li> |
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/Team:GDSYZX-United/Proof">Proof</a></li> |
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/Team:GDSYZX-United/Demonstrate">Demonstrate</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> PARTS <span class="caret"></span></a> | + | <a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United/results">RESULTS</a> |
| + | </li> | ||
| + | <li class="dropdown"> | ||
| + | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown">PARTS<span class="caret"></span></a> | ||
<ul role="menu" class="dropdown-menu"> | <ul role="menu" class="dropdown-menu"> | ||
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/GDSYZX-United/PARTS/parts">Parts</a></li> |
| − | + | ||
| − | + | ||
| − | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> NOTEBOOK <span class="caret"></span></a> | + | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown">NOTEBOOK<span class="caret"></span></a> |
<ul role="menu" class="dropdown-menu"> | <ul role="menu" class="dropdown-menu"> | ||
| − | + | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/day_note">Day notes</a></li> | |
| − | + | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/procedure_record">Procedure record</a></li> | |
| − | + | <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/protocol">Protocol</a></li> | |
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> | + | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown">HUMAN PRACTICE<span class="caret"></span></a> |
<ul role="menu" class="dropdown-menu"> | <ul role="menu" class="dropdown-menu"> | ||
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/Team:GDSYZX-United/HP/Silver">Silver</a></li> |
| − | <li><a href=" | + | <li><a href="https://2016.igem.org/Team:GDSYZX-United/HP/Gold">Gold</a></li> |
| + | <li><a href="https://2016.igem.org/GDSYZX-United/HUMAN_PRACTICE/album">Album</a></li> | ||
</ul> | </ul> | ||
</li> | </li> | ||
<li class="dropdown"> | <li class="dropdown"> | ||
| − | + | <a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown">SAFETY<span class="caret"></span></a> | |
| − | + | <ul role="menu" class="dropdown-menu"> | |
| − | + | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/lab_safety">Lab safety</a></li> | |
| − | + | <li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/safety_of_process">Safety of process</a></li> | |
| − | + | </ul> | |
| − | + | </li> | |
| − | + | <li class="dropdown"> | |
| − | + | <a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United/Entrepreneurship">ENTREPRENEURSHIP</a> | |
| − | + | </li> | |
| − | + | </ul> | |
| − | + | </div> | |
| − | + | </nav> | |
| − | + | ||
| − | + | ||
<div class="row animated fadeInRight"> | <div class="row animated fadeInRight"> | ||
| − | + | <div class="col-sm-12"> | |
<div class="ibox float-e-margins"> | <div class="ibox float-e-margins"> | ||
| − | <div class=" | + | <div class="timeline"> |
| + | <div> | ||
| + | <div class="row"> | ||
| + | <div class="text-center"> | ||
| + | <h1><strong>Day note</strong></h1> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.7.31 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> | |
| − | <small class="text-navy">9:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong></div> |
| − | + | <p><div class="text_font">The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.</div></p> | |
| − | <p>The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.</p> | + | |
</div> | </div> | ||
</div> | </div> | ||
| Line 113: | Line 125: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.7.31 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.7.31</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">13:30</div></small></p> | |
| − | <small class="text-navy">13:30</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>Extract genome from Arabidopsis Thaliana</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR. | We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.1 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.1</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> | |
| − | <small class="text-navy">9:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We use PCR to generate and to amplify our target DNA fragments:promoter cop1,promoter phyB,promoter pif1,gene hhl1,gene pHhl,gene flu.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again. | We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 145: | Line 157: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.2 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.2</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> | |
| − | <small class="text-navy">9:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>Do the PCR again to get our target DNA fragments.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We PCR gene pHhl1,gene hhl1,gene flu successfully. | We PCR gene pHhl1,gene hhl1,gene flu successfully. | ||
We will PCR cop1 together with pif1,phyB tomorrow. | We will PCR cop1 together with pif1,phyB tomorrow. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 162: | Line 174: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.3 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> | |
| − | <small class="text-navy">9:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We use PCR to get cop1,phyB and pif1</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
| − | In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second | + | In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second times PCR. |
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 178: | Line 190: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.3 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">13:30</div></small></p> | |
| − | <small class="text-navy">13:30</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We use a kit box to purify our PCR products,so we can get our genes.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.3 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.3</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> | |
| − | <small class="text-navy">in the afternoon</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ, |
EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong> | EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments. </strong> | ||
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 211: | Line 223: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> | |
| − | <small class="text-navy">9:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We purify our genes from the restriction enzyme systems.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 227: | Line 239: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p> | |
| − | <small class="text-navy">in the morning</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We cut our plasmids psb3c1 with BsaⅠ.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 244: | Line 256: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p> | |
| − | <small class="text-navy">in the morning</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We splice our DNA fragments to get the parts we want using |
a kit box.</strong> | a kit box.</strong> | ||
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 261: | Line 273: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">13:30</div></small></p> | |
| − | <small class="text-navy">13:30</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We purify our parts using a kit box.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 277: | Line 289: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> | |
| − | <small class="text-navy">in the afternoon</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We cut our parts with BsaⅠto |
make sure there will be no loops in the part.</strong> | make sure there will be no loops in the part.</strong> | ||
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 294: | Line 306: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> | |
| − | <small class="text-navy">in the afternoon</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We purify the parts.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 310: | Line 322: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> | |
| − | <small class="text-navy">in the afternoon</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We splice our parts with plasmids.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 326: | Line 338: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> | |
| − | <small class="text-navy">in the afternoon</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We prepare LB broth.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 342: | Line 354: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">19:00</div></small></p> | |
| − | <small class="text-navy">19:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We transform our plasmids into DH5α.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 358: | Line 370: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.4 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.4</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the evening</div></small></p> | |
| − | <small class="text-navy">in the evening</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We inoculate Ecoli DH5α on LB plates and in media.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 374: | Line 386: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.5 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.5</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">13:00</div></small></p> | |
| − | <small class="text-navy">13:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We check the growth of Ecoli on LB plates.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
No conlony was observed. | No conlony was observed. | ||
So we will use the Ecoli in media to do the following experiments. | So we will use the Ecoli in media to do the following experiments. | ||
| − | + | </div></p> | |
| − | </p> | + | |
</div> | </div> | ||
</div> | </div> | ||
| Line 392: | Line 403: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.5 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.5</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> | |
| − | <small class="text-navy">in the afternoon</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We extract plasmids from the solution.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 408: | Line 419: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.6 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> | |
| − | <small class="text-navy">9:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We prepare Arabidopsis protoplasts.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
The concentration of protoplasts is too low,this is because we chose the wrong filter paper. | The concentration of protoplasts is too low,this is because we chose the wrong filter paper. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 424: | Line 435: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.6 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">13:00</div></small></p> | |
| − | <small class="text-navy">13:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We prepare protoplasts again.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging. | We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 440: | Line 451: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.6 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.6</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the afternoon</div></small></p> | |
| − | <small class="text-navy">in the afternoon</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We transfect our plasmids into the protoplasts，and incubate |
the protoplasts in the following light intensity (lux):0,3960,7920,11880. | the protoplasts in the following light intensity (lux):0,3960,7920,11880. | ||
</strong> | </strong> | ||
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 458: | Line 469: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.7 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.7</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">9:00</div></small></p> | |
| − | <small class="text-navy">9:00</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We extract RNA from protoplasts using a kit box.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We did it. | We did it. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 474: | Line 485: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.7 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.7</div></p> |
| − | + | <small class="text-navy"><p><div class="text_font">in the morning</div></small></p> | |
| − | <small class="text-navy">in the morning</small> | + | |
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>We use semiquantitive RT-PCR |
to amplify hhl1 to see how much hhl1 is transcripted into mRNA. | to amplify hhl1 to see how much hhl1 is transcripted into mRNA. | ||
</strong> | </strong> | ||
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We failed to get any hhl1. There may be something wrong with the process of transformation. | We failed to get any hhl1. There may be something wrong with the process of transformation. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
| Line 492: | Line 503: | ||
<div class="timeline-item"> | <div class="timeline-item"> | ||
<div class="row"> | <div class="row"> | ||
| + | <div class="col-xs-1"></div> | ||
<div class="col-xs-3 date"> | <div class="col-xs-3 date"> | ||
| − | <i class="fa fa-file-text"></i> 2016.8.10 | + | <i class="fa fa-file-text"></i><p><div class="text_font"> 2016.8.10</div></p> |
| − | + | ||
</div> | </div> | ||
<div class="col-xs-8 content"> | <div class="col-xs-8 content"> | ||
| − | < | + | <div class="m-b-xs text_font"><strong>PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.</strong> |
| − | </ | + | </div> |
| − | <p> | + | <p><div class="text_font"> |
We will check the result tomorrow. | We will check the result tomorrow. | ||
| − | </p> | + | </div></p> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 05:05, 19 October 2016
Day note
2016.7.31
9:00
Extract genome from Arabidopsis Thaliana
The genome has been degraded greatly,possibly because we didn’t grind the leaves in very low temperature to inhibit the nuclease.We have to do it again.
2016.7.31
13:30
Extract genome from Arabidopsis Thaliana
We extracted it the genome successfully.But the genome has still been degraded a little. (Since we don’t have liquid nitrogen in our lab,so we grinded the leaves in an ice box. )We will use the genome to do the following PCR.
2016.8.1
9:00
We use PCR to generate and to amplify our target DNA fragments:promoter cop1,promoter phyB,promoter pif1,gene hhl1,gene pHhl,gene flu.
We failed to get all our target DNA fragments .We will check the primers and the PCR program ,and then do it again.
2016.8.2
9:00
Do the PCR again to get our target DNA fragments.
We PCR gene pHhl1,gene hhl1,gene flu successfully.
We will PCR cop1 together with pif1,phyB tomorrow.
2016.8.3
9:00
We use PCR to get cop1,phyB and pif1
In the first PCR we get cop 1 and pif1,we get higher concentration of phyB in the second times PCR.
2016.8.3
13:30
We use a kit box to purify our PCR products,so we can get our genes.
We did it.
2016.8.3
in the afternoon
We cut our DNA fragments using restriction enzymes:BsaⅠ,PstⅠ,
EcorⅠ.After this process ,the gene fragments will have one overhang at both ends.The overhangs will help them splice other fragments.
We did it.
2016.8.4
9:00
We purify our genes from the restriction enzyme systems.
We did it.
2016.8.4
in the morning
We cut our plasmids psb3c1 with BsaⅠ.
We did it.
2016.8.4
in the morning
We splice our DNA fragments to get the parts we want using
a kit box.
We did it.
2016.8.4
13:30
We purify our parts using a kit box.
We did it.
2016.8.4
in the afternoon
We cut our parts with BsaⅠto
make sure there will be no loops in the part.
We did it.
2016.8.4
in the afternoon
We purify the parts.
We did it.
2016.8.4
in the afternoon
We splice our parts with plasmids.
We did it.
2016.8.4
in the afternoon
We prepare LB broth.
We did it.
2016.8.4
19:00
We transform our plasmids into DH5α.
We did it.
2016.8.4
in the evening
We inoculate Ecoli DH5α on LB plates and in media.
We did it.
2016.8.5
13:00
We check the growth of Ecoli on LB plates.
No conlony was observed.
So we will use the Ecoli in media to do the following experiments.
2016.8.5
in the afternoon
We extract plasmids from the solution.
We did it.
2016.8.6
9:00
We prepare Arabidopsis protoplasts.
The concentration of protoplasts is too low,this is because we chose the wrong filter paper.
2016.8.6
13:00
We prepare protoplasts again.
We did it.The concentration is much higher,and there is much more sediment of protoplast after centrifuging.
2016.8.6
in the afternoon
We transfect our plasmids into the protoplasts，and incubate
the protoplasts in the following light intensity (lux):0,3960,7920,11880.
We did it.
2016.8.7
9:00
We extract RNA from protoplasts using a kit box.
We did it.
2016.8.7
in the morning
We use semiquantitive RT-PCR
to amplify hhl1 to see how much hhl1 is transcripted into mRNA.
We failed to get any hhl1. There may be something wrong with the process of transformation.
2016.8.10
PCR our target DNA fragments:cop1,pif1,phyB,hhl1,pHhl1-hhl1,flu.
We will check the result tomorrow.
