Difference between revisions of "GDSYZX-United/PARTS/parts"

 
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<ul class="nav navbar-nav navbar-right">
 
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<a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United"> HOME </a>
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<a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <span class="caret"></span></a>
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<li><a href="https://2016.igem.org/GDSYZX-United/TEAM/team_members">Team members</a></li>
 
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/Collaborations">Collarboration</a></li>
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/Attributions">Attribution</a></li>
 
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<a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <span class="caret"></span></a>
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<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/project_description">Project description</a></li>
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/Description">Project description</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/extract _dna">Extract DNA in arabidopsis thaliana</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/extract _dna">Extract DNA in arabidopsis thaliana</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/enzyme_cleavage">Enzyme cleavage</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/enzyme_cleavage">Enzyme cleavage</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/preparation_arabidopsis_protoplast">Preparation of the Arabidopsis protoplast</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/preparation_arabidopsis_protoplast">Preparation of the Arabidopsis protoplast</a></li>
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<li><a href="https://2016.igem.org/GDSYZX-United/PROJECT/result">Result</a></li>
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/Proof">Proof</a></li>
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/Demonstrate">Demonstrate</a></li>
 
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<a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> PARTS <span class="caret"></span></a>
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<a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United/results">RESULTS</a>
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<a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown">PARTS<span class="caret"></span></a>
 
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<li><a href="https://2016.igem.org/GDSYZX-United/PARTS/parts">Parts</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/PARTS/parts">Parts</a></li>
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    <li><a href="https://2016.igem.org/GDSYZX-United/NOTEBOOK/day_note">Day notes</a></li>
 
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<a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <span class="caret"></span></a>
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<li><a href="https://2016.igem.org/GDSYZX-United/HUMAN_PRACTICE/human_practice">Human Practice</a></li>
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/HP/Silver">Silver</a></li>
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<li><a href="https://2016.igem.org/Team:GDSYZX-United/HP/Gold">Gold</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/HUMAN_PRACTICE/album">Album</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/HUMAN_PRACTICE/album">Album</a></li>
 
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<a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> SAFETY <span class="caret"></span></a>
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<li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/lab_safety">Lab safety</a></li>
 
<li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/lab_safety">Lab safety</a></li>
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<li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/safety_of_process">Safety_of_process</a></li>
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<li><a href="https://2016.igem.org/GDSYZX-United/SAFETY/safety_of_process">Safety of process</a></li>
 
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<a aria-expanded="false" role="button" href="#" class="dropdown-toggle" data-toggle="dropdown"> ENTREPRENEURSHIP <span class="caret"></span></a>
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<a aria-expanded="false" role="button" href="https://2016.igem.org/Team:GDSYZX-United/Entrepreneurship">ENTREPRENEURSHIP</a>
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<li><a href="#">1</a></li>
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<li><a href="#">2</a></li>
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<li><a href="#">3</a></li>
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<li><a href="#">4</a></li>
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                 <div class="ibox float-e-margins">
 
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<h1><strong>Parts</strong></h1>
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                                    <h2>Part1:PHhl1-hhl1-flu</h2>
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<p><div class="text_font">This biobrick consists of a complete functional protein HHL1 coding sequence in Arabidopsis ,gene hhl1,fused with a flu sequence as a reporter.Gene hhl1 encodes protein HHL1,which is produced and distributed among chloroplasts(especailly grana core-,grana margin and stroma lamellae enriched thylokoid membranes) to function in protecting and repairing photosystemⅡfrom photodamage. pHhl1 is the promoter of gene hhl1 in wild type Arabidopsis,the promoter may be regulated by the concentration of certain protein differs from before and after photodamage takes place in photosystemⅡ. </div></p>
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<div class="text_font"><font color="blue">In order to examine the efficiency of this device,</font>this biobrick has been taken into the nucleus of protoplasts of Arabiodopsis using psb1c3 plasmid as its carrier.RT-PCR is done to the transcribed mRNA of gene hhl1 to get cDNA,then we use the ultraviolet spectrophotometer to measure the concentration of cDNA we got.The concentration data is used to calculate the relative expression level of gene hhl1 compared to part2,3,4.</div></p>
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<p><div class="text_font">From the result,part1 has no significent change in expression level of mRNA in growth light(100lux) and in high light(1200lux).This means that the promoter pHhl1 is repressed in these light intensities so the expression level is quite low.</div></p>
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                                    <h2><strong>Part2:pPhyB-hhl1-flu</strong></h2>
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<p><div class="text_font">This biobrick consists of a photosensitive promoter pPhyB, and protein coding sequence hhl1 fused with gene flu as a reporter. pPhyB is the promoter of gene phyB, which encodes phytochrome B. PhytochromeB is a photoreceptor which is sensitive to red/far-red light(wavelength:660-730nm).This biobrick is constructed to produce more protein HHL1 in chloroplasts to help protect photosystemⅡ before photodamage takes place.</div></p>
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                                    <h2><strong>Part3:pPif1-hhl1-flu</strong></h2>
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<p><div class="text_font">This biobrick consists of a photosensitive promoter pPif1,and protein coding sequence hhl1 fused with gene flu as a reporter.This biobrick is constructed to produce more protein HHL1 in chloroplasts to help protect photosystemⅡ before photodamage takes place.</div></p>
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                                    <h2><strong>Part4:pCop1-hhl1-flu</strong></h2>
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<p><div class="text_font">
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<p><div class="text_font">This biobrick consists of a photosensitive promoter pCop1,and protein coding sequence hhl1 fused with gene flu as a reporter.This biobrick is constructed to produce more protein HHL1 in chloroplasts to help protect photosystemⅡ before photodamage takes place.</div></p>
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<table class= "table" style="text-align:center">
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<thead>
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<th style="text-align:center">Name</th>
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<th style="text-align:center">Type</th>
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<th style="text-align:center">Description</th>
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<th style="text-align:center">Length (bp)</th>
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<tbody>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089001">BBa_K2089001</a></td>
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<td>Regulatory</td>
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<td>pif promoter from Arabidopsis</td>
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<td>1497</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089002">BBa_K2089002</a></td>
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<td>Regulatory</td>
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<td>cop1 promoter from Arabidopsis</td>
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<td>1849</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089003">BBa_K2089003</a></td>
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<td>Regulatory</td>
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<td>phyB promoter from Arabidopsis</td>
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<td>813</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089004">BBa_K2089004</a></td>
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<td>Regulatory</td>
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<td>pHHL from Arabidopsis</td>
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<td>1500</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089005">BBa_K2089005</a></td>
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<td>Coding</td>
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<td>HHL1 protein from Arabidopsis</td>
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<td>696</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089006">BBa_K2089006</a></td>
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<td>Composite</td>
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<td>pHHL-hhl1</td>
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<td>3117</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089007">BBa_K2089007</a></td>
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<td>Composite</td>
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<td>Ppif1-hhl1</td>
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<td>2199</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089008">BBa_K2089008</a></td>
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<td>Composite</td>
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<td>Pcop1-hhl1</td>
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<td>2551</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089009">BBa_K2089009</a></td>
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<td>Composite</td>
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<td>PphyB-hhl1</td>
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<td>1515</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089010">BBa_K2089010</a></td>
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<td>Composite</td>
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<td>Ppif1-RBS-RFP-terminator</td>
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<td>2366</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089011">BBa_K2089011</a></td>
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<td>Composite</td>
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<td>Pcop1-RBS-RFP-terminator</td>
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<td>2718</td>
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</tr>
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<tr>
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<td><a href="http://parts.igem.org/Part:BBa_K2089012">BBa_K2089012</a></td>
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<td>Composite</td>
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<td>PphyB-RBS-RFP-terminator</td>
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<td>1682</td>
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</tr>
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</tbody>
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</table>
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</div></p>
 
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Latest revision as of 05:47, 19 October 2016

Parts

Name Type Description Length (bp)
BBa_K2089001 Regulatory pif promoter from Arabidopsis 1497
BBa_K2089002 Regulatory cop1 promoter from Arabidopsis 1849
BBa_K2089003 Regulatory phyB promoter from Arabidopsis 813
BBa_K2089004 Regulatory pHHL from Arabidopsis 1500
BBa_K2089005 Coding HHL1 protein from Arabidopsis 696
BBa_K2089006 Composite pHHL-hhl1 3117
BBa_K2089007 Composite Ppif1-hhl1 2199
BBa_K2089008 Composite Pcop1-hhl1 2551
BBa_K2089009 Composite PphyB-hhl1 1515
BBa_K2089010 Composite Ppif1-RBS-RFP-terminator 2366
BBa_K2089011 Composite Pcop1-RBS-RFP-terminator 2718
BBa_K2089012 Composite PphyB-RBS-RFP-terminator 1682