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| {{:Team:Tianjin/Templates/MaterialTheme|}} | | {{:Team:Tianjin/Templates/MaterialTheme|}} |
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− | {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Note/Consortium/camarts.css}} | + | |
| + | {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Note/R-R/camarts.css}} |
| {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Experiment/css/font.css}} | | {{:Team:Tianjin/Templates/AddCSS|:Team:Tianjin/Experiment/css/font.css}} |
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| <html lang="en"> | | <html lang="en"> |
| + | <style> |
| + | #Notes{ |
| + | color: inherit; |
| + | background-color: rgba(255, 255, 255, 0.1); |
| + | } |
| + | #content article{margin:0;padding:0;} |
| + | </style> |
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| <meta charset="utf-8" /> | | <meta charset="utf-8" /> |
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| <header id="main-header" role="banner"> | | <header id="main-header" role="banner"> |
− | | + | <style> |
| + | .note-content,.note-content2,.note-content3,.note-content4,.note-content5,.note-content6,.note-content7,.note-content8,.note-content9,.note-content10,.note-content li,.note-content2 li,.note-content3 li,.note-content4 li,.note-content5 li,.note-content6 li,.note-content7 li,.note-content8 li,.note-content9 li,.note-content10 li{ |
| + | font-family:Arial; |
| + | font-size:18px; |
| + | text-align:justify; |
| + | } |
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| <article id="post-4252" class="post-4252 post type-post status-publish format-standard has-post-thumbnail hentry category-150 tag-174 tag-xinjiang tag-173"> | | <article id="post-4252" class="post-4252 post type-post status-publish format-standard has-post-thumbnail hentry category-150 tag-174 tag-xinjiang tag-173"> |
| <header class="entry-header"> | | <header class="entry-header"> |
− | <div class="entry-title" align="center" >Notes</div> | + | <div class="entry-title" align="center" >Notes for Bacterial Consortium</div> |
| </header><!-- .entry-header --> | | </header><!-- .entry-header --> |
| <h1 class="entry-title">Week1(7/24/2016-7/30/2016)</h1> | | <h1 class="entry-title">Week1(7/24/2016-7/30/2016)</h1> |
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| <b><h1 style="font-size:108%"> Jul.27th</b></h1> | | <b><h1 style="font-size:108%"> Jul.27th</b></h1> |
| <div style="padding-left:32px;">1.Extract 10 ml LB medium to 5 test tubes and add 0μl, 50μl,, 100μl,, 250μl,, 500μl EG to them, respectively.<br/> | | <div style="padding-left:32px;">1.Extract 10 ml LB medium to 5 test tubes and add 0μl, 50μl,, 100μl,, 250μl,, 500μl EG to them, respectively.<br/> |
− | 2.Add 5μl becteria solution of <i>Pseudomonas putida KT2440</i> to five test tubes above.<br/> | + | 2.Add 5μl bacteria solution of <i>Pseudomonas putida KT2440</i> to five test tubes above.<br/> |
| 3.Culture them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub>. <br/> | | 3.Culture them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub>. <br/> |
| <br/></div> | | <br/></div> |
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| <b><h1 style="font-size:108%"> Aug.9th</b></h1> | | <b><h1 style="font-size:108%"> Aug.9th</b></h1> |
| <div style="padding-left:32px;">1.Extract 5ml YPD medium to 8 test tubes, respectively.<br/> | | <div style="padding-left:32px;">1.Extract 5ml YPD medium to 8 test tubes, respectively.<br/> |
− | 2.Added becteria solution as following table (use two tubes each group)</div> | + | 2.Added bacteria solution as following table (use two tubes each group)</div> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/4/4b/T--Tianjin--table_8.9-1.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/4/4b/T--Tianjin--table_8.9-1.png"></div> |
| <div style="padding-left:32px;">3.Cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub></div> | | <div style="padding-left:32px;">3.Cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub></div> |
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| <b><h1 style="font-size:108%"> Aug.10th</b></h1> | | <b><h1 style="font-size:108%"> Aug.10th</b></h1> |
| <div style="padding-left:32px;">1.Prepared 300ml W0 medium and add 1.5g Yeast Extract, then regarded it W1 medium.<br/> | | <div style="padding-left:32px;">1.Prepared 300ml W0 medium and add 1.5g Yeast Extract, then regarded it W1 medium.<br/> |
− | 2.Extractd 8ml W1 medium to 16 test tubes, respectively.<br/> | + | 2.Extracted 8ml W1 medium to 16 test tubes, respectively.<br/> |
− | 3.Added becteria solution as following table (use two tubes each group).</div> | + | 3.Added bacteria solution as following table (use two tubes each group).</div> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> |
| <div style="padding-left:32px;">4.Cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>.</div> | | <div style="padding-left:32px;">4.Cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>.</div> |
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| <b><h1 style="font-size:108%"> Aug.12th</b></h1> | | <b><h1 style="font-size:108%"> Aug.12th</b></h1> |
| <div style="padding-left:32px;">1.Prepared 200ml W0 medium and add 0.4g Urea, then regarded it W2 medium.<br/> | | <div style="padding-left:32px;">1.Prepared 200ml W0 medium and add 0.4g Urea, then regarded it W2 medium.<br/> |
− | 2.Extractd 8ml W2 medium to 16 test tubes, respectively.<br/> | + | 2.Extracted 8ml W2 medium to 16 test tubes, respectively.<br/> |
− | 3.Added becteria solution as following table (use two tubes each group)<br/> | + | 3.Added bacteria solution as following table (use two tubes each group)<br/> |
| </div> | | </div> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> |
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| <div style="padding-left:32px;">1.Prepared 200ml W0 medium and add 0.4g KNO3, then regarded it W3 medium.<br/> | | <div style="padding-left:32px;">1.Prepared 200ml W0 medium and add 0.4g KNO3, then regarded it W3 medium.<br/> |
− | 2.Extractd 8ml W3 medium to 16 test tubes, respectively.<br/> | + | 2.Extracted 8ml W3 medium to 16 test tubes, respectively.<br/> |
− | 3.Added becteria solution as following table (use two tubes each group)<br/> | + | 3.Added bacteria solution as following table (use two tubes each group)<br/> |
| </div> | | </div> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> |
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| <b><h1 style="font-size:108%"> Aug.13th</b></h1> | | <b><h1 style="font-size:108%"> Aug.13th</b></h1> |
| <div style="padding-left:32px;">1.Extract 5ml W medium to 6 test tubes and add 20μL, 50μL, 80μL EG to them, respectively;<br/> | | <div style="padding-left:32px;">1.Extract 5ml W medium to 6 test tubes and add 20μL, 50μL, 80μL EG to them, respectively;<br/> |
− | 2.Add 10 μL becteria solution of <i>Pseudomonas putida KT2440</i> to the six test tubes, respectively;<br/> | + | 2.Add 10 μL bacteria solution of <i>Pseudomonas putida KT2440</i> to the six test tubes, respectively;<br/> |
| 3. Cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub>.<br/> | | 3. Cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub>.<br/> |
| </div> | | </div> |
| <br/> | | <br/> |
− | <div style="padding-left:32px;">Added becteria solution to W2, W3 medium as following table (use two tubes each group)</div> | + | <div style="padding-left:32px;">Added bacteria solution to W2, W3 medium as following table (use two tubes each group)</div> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/d/d5/T--Tianjin--table_8.13.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/d/d5/T--Tianjin--table_8.13.png"></div> |
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| <b><h1 style="font-size:108%"> Aug.15th</b></h1> | | <b><h1 style="font-size:108%"> Aug.15th</b></h1> |
| <div style="padding-left:32px;">1.Prepared 200ml W0 medium and add 0.6g sucrose, then regarded it W4 medium.<br/> | | <div style="padding-left:32px;">1.Prepared 200ml W0 medium and add 0.6g sucrose, then regarded it W4 medium.<br/> |
− | 2.Extractd 8ml W4 medium to 16 test tubes, respectively.<br/> | + | 2.Extracted 8ml W4 medium to 16 test tubes, respectively.<br/> |
− | 3.Added becteria solution as following table (use two tubes each group)<br/> | + | 3.Added bacteria solution as following table (use two tubes each group)<br/> |
| </div> | | </div> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> |
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| <b><h1 style="font-size:108%"> Aug.16th</b></h1> | | <b><h1 style="font-size:108%"> Aug.16th</b></h1> |
| <div style="padding-left:32px;">Prepared 200ml W0 medium.<br/> | | <div style="padding-left:32px;">Prepared 200ml W0 medium.<br/> |
− | 2.Extractd 8ml W0 medium to 16 test tubes, respectively.<br/> | + | 2.Extracted 8ml W0 medium to 16 test tubes, respectively.<br/> |
− | 3.Added becteria solution as following table (use two tubes each group)<br/> | + | 3.Added bacteria solution as following table (use two tubes each group)<br/> |
| </div> | | </div> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> |
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| <div style="padding-left:32px;">We aimed to put the left-tet-right gene and plasmid which have λ-red gene into the the genome of E.coli. After two screening, we could gain the target strains which knocked out the atpF and atpH gene.</div> | | <div style="padding-left:32px;">We aimed to put the left-tet-right gene and plasmid which have λ-red gene into the the genome of E.coli. After two screening, we could gain the target strains which knocked out the atpF and atpH gene.</div> |
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− | </li> | + | </li> |
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| <b><h1 style="font-size:108%"> Aug.22th</b></h1> | | <b><h1 style="font-size:108%"> Aug.22th</b></h1> |
| 1.Prepared 200ml W0 medium and add 0.3g NaOAc, then regarded it W7 medium.<br/> | | 1.Prepared 200ml W0 medium and add 0.3g NaOAc, then regarded it W7 medium.<br/> |
− | 2.Extractd 8ml W7 medium to 16 test tubes, respectively.<br/> | + | 2.Extracted 8ml W7 medium to 16 test tubes, respectively.<br/> |
− | 3.Added becteria solution as following table (use two tubes each group)<br/> | + | 3.Added bacteria solution as following table (use two tubes each group)<br/> |
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| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> |
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| </div> | | </div> |
| <a class="expand-btn4">Show More</a> | | <a class="expand-btn4">Show More</a> |
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| <li><h1 style="font-size:135%">Optimization of Culture Conditions</h1><br/> | | <li><h1 style="font-size:135%">Optimization of Culture Conditions</h1><br/> |
| <b><h1 style="font-size:108%"> Sep.12th</b></h1> | | <b><h1 style="font-size:108%"> Sep.12th</b></h1> |
− | <div style="padding-left:32px;">Optimize the growing environment of Bacillus stubtilis by change medium components.Firstly, we prepared 400ml W medium, then, we devided the medium into four pieces averagely and number them No.1, No.2, No.3, No.4. Next, we added 0.1g NaCl, 0.1g NH4Cl, 0.3g sucrose to No.2, No.3, No.4, respectively. After that, we extracted 5 ml No.1, No.2, No.3, No.4 and added them to four test tubes, then, we added 10 μL bacteria solution of <i>Bacillus stubtilis 168</i> to four test tubes, respectively. Eventually, we cultured them at 37℃ and check growing situation.</div> | + | <div style="padding-left:32px;">Optimize the growing environment of Bacillus stubtilis by change medium components.Firstly, we prepared 400ml W medium, then, we devided the medium into four pieces averagely and number them No.1, No.2, No.3, No.4. Next, we added 0.1g NaCl, 0.1g NH4Cl, 0.3g sucrose to No.2, No.3, No.4, respectively. After that, we Extracted 5 ml No.1, No.2, No.3, No.4 and added them to four test tubes, then, we added 10 μL bacteria solution of <i>Bacillus stubtilis 168</i> to four test tubes, respectively. Eventually, we cultured them at 37℃ and check growing situation.</div> |
| </li> | | </li> |
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| 1.Prepared 200ml W0 medium and add 0.2g NH4Cl, then regarded it W8 medium.<br/> | | 1.Prepared 200ml W0 medium and add 0.2g NH4Cl, then regarded it W8 medium.<br/> |
− | 2.Extractd 10ml W8 medium to 16 test tubes, respectively.<br/> | + | 2.Extracted 10ml W8 medium to 16 test tubes, respectively.<br/> |
− | 3.Added becteria solution as following table (use two tubes each group)<br/> | + | 3.Added bacteria solution as following table (use two tubes each group)<br/> |
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| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/7/7f/T--Tianjin--table_8.22.png"></div> |
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| </div> | | </div> |
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| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/f/f4/T--Tianjin--table_9.19-1.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/f/f4/T--Tianjin--table_9.19-1.png"></div> |
− | 3. Extractd 5ml W9, W10, W11, W12, W13 medium to 40 test tubes, respectively.<br/> | + | 3. Extracted 5ml W9, W10, W11, W12, W13 medium to 40 test tubes, respectively.<br/> |
− | 4. Added becteria solution as following table (use two tubes each group)<br/> | + | 4. Added bacteria solution as following table (use two tubes each group)<br/> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/d/d1/T--Tianjin--table_9.19-2.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/d/d1/T--Tianjin--table_9.19-2.png"></div> |
| 5. cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>.<br/> | | 5. cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>.<br/> |
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| <b><h1 style="font-size:108%"><br/> Sep.22th</b></h1> | | <b><h1 style="font-size:108%"><br/> Sep.22th</b></h1> |
| 1. Use five mediums of Sep.19th and W0, W8 medium of Sep.16th.<br/> | | 1. Use five mediums of Sep.19th and W0, W8 medium of Sep.16th.<br/> |
− | 2. Extractd 5ml W0, W8, W9, W10, W11, W12, W13 medium to 56 test tubes, respectively.<br/> | + | 2. Extracted 5ml W0, W8, W9, W10, W11, W12, W13 medium to 56 test tubes, respectively.<br/> |
− | 3. Added becteria solution as following table (use two tubes each group)<br/> | + | 3. Added bacteria solution as following table (use two tubes each group)<br/> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/0/04/T--Tianjin--table_9.22.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/0/04/T--Tianjin--table_9.22.png"></div> |
| 4. cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>. | | 4. cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>. |
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| 2. devided the medium into four pieces averagely and add chemicals as following table<br/> | | 2. devided the medium into four pieces averagely and add chemicals as following table<br/> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/c/c3/T--Tianjin--table_9.23.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/c/c3/T--Tianjin--table_9.23.png"></div> |
− | 3. Extractd 5ml W0, W14, W15, W16 medium to 34 test tubes, respectively.<br/> | + | 3. Extracted 5ml W0, W14, W15, W16 medium to 34 test tubes, respectively.<br/> |
− | 4. Added becteria solution as following table (use two tubes each group)<br/> | + | 4. Added bacteria solution as following table (use two tubes each group)<br/> |
| <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/b/bc/T--Tianjin--table_9.23-2.png"></div> | | <div style="text-align:center"><img src="https://static.igem.org/mediawiki/2016/b/bc/T--Tianjin--table_9.23-2.png"></div> |
| 5. cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>.<br/> | | 5. cultured them at 30℃ & 200 rpm, checked the bacterial concentration at OD<sub>600</sub> and detected the concentration of TPA by UV at OD<sub>242</sub>.<br/> |
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| <b><h1 style="font-size:108%"> Sep.19th</b></h1> | | <b><h1 style="font-size:108%"> Sep.19th</b></h1> |
− | <div style="padding-left:32px;">Construct plamid of MHETase without success.<br/> | + | <div style="padding-left:32px;">Construct plasmid of MHETase without success.<br/> |
| Cultivated MHETase transformed <i>B.stubtilis 168</i> on Erythromycin containing LB plates to observe, without success. | | Cultivated MHETase transformed <i>B.stubtilis 168</i> on Erythromycin containing LB plates to observe, without success. |
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| </div> | | </div> |
− | <div class="side-nav">
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− | <ul>
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− | <li><a class="topLink" href="#Topp" style="color:#5555FF">Top</a></li>
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− | <li><a class="topLink" href="#Week1" style="color:#5555FF">Week1</a></li>
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− | <li><a class="topLink" href="#Week2" style="color:#5555FF">Week2</a></li>
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− | <li><a class="topLink" href="#Week3" style="color:#5555FF">Week3</a></li>
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− | <li><a class="topLink" href="#Week4" style="color:#5555FF">Week4</a></li>
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− | <li><a class="topLink" href="#Week5" style="color:#5555FF">Week5</a></li>
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− | <li><a class="topLink" href="#Week6" style="color:#5555FF">Week6</a></li>
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− | <li><a class="topLink" href="#Week7" style="color:#5555FF">Week7</a></li>
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− | <li><a class="topLink" href="#Week8" style="color:#5555FF">Week8</a></li>
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− | <li><a class="topLink" href="#Week9" style="color:#5555FF">Week9</a></li>
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− | <li><a class="topLink" href="#Week10" style="color:#5555FF">Week10</a></li>
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− | <div class="alert alert-info">
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− | <div class="container-fluid">
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− | <div class="alert-icon">
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− | <b>Notice:</b> This page is currently under construction. Contents in this page are temporaory and will be modified several times before the final release. — 2016 iGEM Team Tianjin
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| <!-- Main JS --> | | <!-- Main JS --> |
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− | var x = 600;
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− | var y = 2000;
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− | for ( var i = 1; i < 22; i++ ){
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− | var rand = parseInt(Math.random() * (x - y + 1) + y);
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− | var randpic = "http://randomimage.setgetgo.com/get.php?width=" + rand;
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− | $("#MemberName" + i).attr("src", randpic);
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− | }
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− | </script>
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− | <script type="text/javascript">
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| </script> | | </script> |
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| </script> | | </script> |
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− | {{:Team:Tianjin/Templates/AddJS|:Team:Tianjin/Attribution/js/camarts.js}}
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| {{:Team:Tianjin/Templates/Sponsor|}} | | {{:Team:Tianjin/Templates/Sponsor|}} |