Difference between revisions of "Team:BostonU/Safety"

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<div style = "font-size:300%; padding: 75px 50px 3px 50px; text-align:center; color:#0071A7;">BostonU Project Gemini Safety Review</div>
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<p style = "font-size:150%; padding:5px 150px 10px 150px; color:#0071A7;">The greatest safety concerns that arose during our project surrounded the chassis used for both cloning and assaying our systems.</p>
  
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<p style = "font-size:150%; padding:5px 150px 10px 150px; color:#0071A7;">Specifically, our chassis encompassed two distinct biological safety levels: BSL1 and BSL2. Each level requires different safety protocols as well training, which students received from both the RIMS (Research Information Management System) program at Boston University and their mentors.</p>
  
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p style = "font-size:150%; padding:5px 150px 10px 150px; color:#0071A7;">All cloning experiments were performed using Top 10 Chemically Competent <i>E. coli</i> on the benches of the BSL1 area of the laboratory. All scientists wore pants, closed toed shoes, lab coats, and gloves while working in these areas.</p>
  
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<p style = "font-size:150%; padding:5px 150px 10px 150px; color:#0071A7;">Our samples were either stored in parafilmed plates in the cold room, in -80 centigrade stasis, or were bleached. All chemicals used during the cloning process (i.e. miniprep waste and ethidium bromide gels) were disposed of in appropriately labeled waste containers located around the lab. Boston University has a solid waste management system which complies entirely with biological and chemicals disposal requirements.</p>
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<p style = "font-size:150%; padding:5px 150px 10px 150px; color:#0071A7;">The work with BSL2 materials centered around HEK293FT cells. While HEK cells are generally a BSL1 cell line, the addition of a SV40 virus into our cells increased the biological safety concern. SV40 poses a cancer risk to scientists who choose to work with it. The same personal protective equipment required for BSL1 were worn in the BSL2 Tissue Culture room, with new gloves being changed every time students stepped out of each lab space. Students were also required to wear state certified eye protection. In addition to bleaching all samples upon the completion of experiments, all work was conducted within biological hoods with media traps. Every surface within those hoods was sterilized both preceding and at the conclusion of work with 70% ethanol.</p>
  
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<h5>Safe Project Design</h5>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h5>Safe Lab Work</h5>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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<h5>Safe Shipment</h5>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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Latest revision as of 19:44, 19 October 2016


BostonU Project Gemini Safety Review


The greatest safety concerns that arose during our project surrounded the chassis used for both cloning and assaying our systems.

Specifically, our chassis encompassed two distinct biological safety levels: BSL1 and BSL2. Each level requires different safety protocols as well training, which students received from both the RIMS (Research Information Management System) program at Boston University and their mentors.

All cloning experiments were performed using Top 10 Chemically Competent E. coli on the benches of the BSL1 area of the laboratory. All scientists wore pants, closed toed shoes, lab coats, and gloves while working in these areas.

Our samples were either stored in parafilmed plates in the cold room, in -80 centigrade stasis, or were bleached. All chemicals used during the cloning process (i.e. miniprep waste and ethidium bromide gels) were disposed of in appropriately labeled waste containers located around the lab. Boston University has a solid waste management system which complies entirely with biological and chemicals disposal requirements.

The work with BSL2 materials centered around HEK293FT cells. While HEK cells are generally a BSL1 cell line, the addition of a SV40 virus into our cells increased the biological safety concern. SV40 poses a cancer risk to scientists who choose to work with it. The same personal protective equipment required for BSL1 were worn in the BSL2 Tissue Culture room, with new gloves being changed every time students stepped out of each lab space. Students were also required to wear state certified eye protection. In addition to bleaching all samples upon the completion of experiments, all work was conducted within biological hoods with media traps. Every surface within those hoods was sterilized both preceding and at the conclusion of work with 70% ethanol.