Difference between revisions of "Team:Tianjin"

 
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                     <img class="img-responsive" src="https://static.igem.org/mediawiki/2016/1/1e/TeamTianjin_BG_Test.jpg" alt="TeamTianjin Back Ground">
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                     <img class="img-responsive headerbackground" src="https://static.igem.org/mediawiki/2016/b/b4/T--Tianjin--background_new.jpg" alt="Team Tianjin Background">
 
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                            <h1>Team Tianjin</h1>
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                            <h1 class="maintitletext" style="margin-top:15px;margin-bottom:25px">Plasterminator</h1>
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                                <div class="separator line-separator">∎</div>
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                      <!--      <h5>"Plastic tastes good."</h5> -->
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                            <h5>"Plastics taste good." &nbsp; --- &nbsp; <i>Yeast</i></h5>
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                                <h2>A brief description</h2>
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                                 <p class="TeamTianjin-text-main">
 
                                 <p class="TeamTianjin-text-main">
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                                     This March our team paid much attention to an article <i class="ref-title">A bacterium that degrades and assimilates poly(ethylene terephthalate)</i><a class="btn popover-info ref-title-link" data-toggle="popover" data-placement="top" title="Reference" data-content="Yoshida, Shosuke, et al. 'A bacterium that degrades and assimilates poly (ethylene terephthalate).' Science 351.6278 (2016): 1196-1199.">1</a> published in Science in the same month. A new kind of bacteria that can decompose PET was found and studied in detail. We plan to express its unique genes in some commonly used mode organisms such as yeasts and E.colis to enhance its activities of decomposition significantly since they are relatively low at present.
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                                     Since 1964, plastics production has increased 20-fold, reaching 311 million tonnes in 2014, the equivalent of more than 900 Empire State Buildings. Plastics production is expected to double again in 20 years and almost quadruple by 2050.
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                                 </p>
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                                  <a class="btn popover-info ref-title-link" data-toggle="popover" data-placement="top" title="Source" data-content="The New Plastics Economy: Rethinking the future of plastics (World Economic Forum, 2016)">1</a>
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                                 </p><br/><br/><br/>
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                                    <div class="revealOnScroll TeamTianjin-text-animNum" data-animation="fadeInUp" data-timeout="5"><p class="NumHide" data-num="20">20</p><span class="NumHide">Folds</span></div>
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                                    <div class="revealOnScroll TeamTianjin-text-animNum" data-animation="fadeInUp" data-timeout="5"><p class="NumHide" data-num="311">311</p><span class="NumHide">MT</span></div>
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                                <img class="img-responsive" src="https://static.igem.org/mediawiki/2016/2/26/T--Tianjin--AboutPlastic.svg" alt="Overview Fig1">
 
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                                    <h2>Polyethylene  terephthalate</h2>
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                                    <p class="TeamTianjin-text-main">
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                                        Poly(ethylene terephthalate) (PET) is one of the most widely used plastic worldwide. However, the durability of PET results in its difficulty to be degraded which leads to a global accumulation of plastic waste.
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                                    <p class="TeamTianjin-text-main">
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                                        Many solutions have been brought up in dealing with the plastic waste. Compared to the traditional chemical recycling processes, enzymatic hydrolysis of PET is presently evaluated as an environmentally friendly strategy for recycling post-consumer PET wastes. And during the last 15 years, many natural enzymes extracted from microorganisms have been found to be capable of decomposing PET.
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                                    </p><br/>
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                                    <h2>Inspiration</h2>
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                                    <p class="TeamTianjin-text-main">
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                                        In recent decades, many labs around the world have proposed a variety of ways to degrade PET biologically. The most inspiring one is the biodegradation ability of a recently found bacterial, called <i class="emphasize">Ideonella sakaiensis</i> <span class="emphasize">201-F6</span>, by Shosuke Yoshida and his colleagues from Japan, which has been studied and published in <i class="ref-title">Science</i> this March<a class="btn popover-info ref-title-link" data-toggle="popover" data-placement="top" title="Reference" data-content="Yoshida, Shosuke, et al. 'A bacterium that degrades and assimilates poly (ethylene terephthalate).' Science 351.6278 (2016): 1196-1199.">2</a>. They analyzed the degrading pathways and isolated two kinds of enzymes, PETase and MHETase. The PETase degrades PET into MHET (mono(2-hydroxyethyl) terephthalic acid) and MHETase degrades MHET into TPA (terephthalic acid) and EG (ethylene glycol).
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                                    <div class="Bottle" data-para="0.8"><img  src="https://static.igem.org/mediawiki/2016/5/58/T--Tianjin--HomepageBottle2.png" class="img-responsive" width="60%" alt="Bottle 2"></div>
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                                    <div class="Bottle" data-para="1"><img  src="https://static.igem.org/mediawiki/2016/6/6f/T--Tianjin--HomepageBottle3.png" class="img-responsive" width="70%" alt="Bottle 3"></div>
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            <img class="img-responsive" src="https://static.igem.org/mediawiki/2016/f/fd/T--Tianjin--ProjectDescription.jpg" alt="Team Tianjin Project Overview">
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                                 <h2>Current situation</h2>
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                                 <h2>Our Solution</h2>
 
                                 <p class="TeamTianjin-text-main">
 
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                                    We have synthesized the gene sequences of the PETase and MHETase based on the supplementary materials of the original paper after several months’ literature reviewing. And we began several preliminary experiments to figure out if those exogenous genes could be well expressed in the host cells. We decide to enhance the activities of these two enzymes via surface display, protein scaffold and fusion expression. Another way to enhance the rate of reactions is to put the first (hydrolysis of PET) and the second step (hydrolysis of MHET) together by cascade catalysis.
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                                  We synthetize the two genes according to the sequences from NCBI, and our project aims to improve the degrading abilities of the two enzymes, especially the key enzyme, PETase, which degrades macromolecule PET with significantly low rate (60mg PET film (20 × 15 × 0.2 mm) was degraded totally by PETase after 60 days according to the Supplementary Materials<a class="btn popover-info ref-title-link" href="http://science.sciencemag.org/content/suppl/2016/03/09/351.6278.1196.DC1">3</a>). We applied <span class="emphasize">Protein Engineering</span> stategy to rationally design the enzyme. We designed a <span class="emphasize">Microbial Consortia</span> to degrade PET and its products TPA and EG completely, as well as a Reporting and Regulation system, <span class="emphasize">R-R System</span> to make the expression visible and controllable. When we assay the enzyme activities, we use unconventional method, <span class="emphasize">Cell-Free Protein Synthesis</span>.
 
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                                 <a class="projecticon" href="https://2016.igem.org/Team:Tianjin/Experiment/Protein_Engineering"><img class="img-responsive" data-animation="zoomIn" src="https://static.igem.org/mediawiki/2016/8/8a/T--Tianjin--Protein_Engineering_ICON.svg" alt="Protein Engineering"></a><h4>Protein Engineering</h4><br/>
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                                    We hope to construct a system that can efficiently express and secrete (or display) these two enzymes. The system will be able to hydrolyze PET with a much higher rate than the Ideonella sakaiensis reported in the thesis.  
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                                <a class="projecticon" href="https://2016.igem.org/Team:Tianjin/Experiment/Consortium"><img class="img-responsive" src="https://static.igem.org/mediawiki/2016/4/49/T--Tianjin--Consortium_ICON.svg"  alt="Microbial Consortia"></a><h4>Microbial Consortia</h4><br/>
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                                <a class="projecticon" href="https://2016.igem.org/Team:Tianjin/Experiment/R-R"><img class="img-responsive" src="https://static.igem.org/mediawiki/2016/e/e0/T--Tianjin--Reporting_Regulation_ICON.svg" alt="R-R System"></a><h4>Reporting-Regulation System</h4><br/>
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                                 <a class="projecticon" href="https://2016.igem.org/Team:Tianjin/Experiment/CFPS"><img class="img-responsive" src="https://static.igem.org/mediawiki/2016/5/5d/T--Tianjin--Cell_Free_System_ICON.svg"  alt="Cell-Free Protein Expression System"></a><h4>Cell-Free Protein Expression System</h4><br/>
 
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  <b>Notice:</b> This page is currently under construction. Contents in this page are temporaory and will be modified several times before the final release. &nbsp;&nbsp;&nbsp; &#8212; 2016 iGEM Team Tianjin
 
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Latest revision as of 20:11, 19 October 2016

TEAM TIANJIN

LOGO


Team Tianjin Background

Plasterminator

Since 1964, plastics production has increased 20-fold, reaching 311 million tonnes in 2014, the equivalent of more than 900 Empire State Buildings. Plastics production is expected to double again in 20 years and almost quadruple by 2050. 1




20

Folds

311

MT
Overview Fig1

Polyethylene terephthalate

Poly(ethylene terephthalate) (PET) is one of the most widely used plastic worldwide. However, the durability of PET results in its difficulty to be degraded which leads to a global accumulation of plastic waste.

Many solutions have been brought up in dealing with the plastic waste. Compared to the traditional chemical recycling processes, enzymatic hydrolysis of PET is presently evaluated as an environmentally friendly strategy for recycling post-consumer PET wastes. And during the last 15 years, many natural enzymes extracted from microorganisms have been found to be capable of decomposing PET.


Inspiration

In recent decades, many labs around the world have proposed a variety of ways to degrade PET biologically. The most inspiring one is the biodegradation ability of a recently found bacterial, called Ideonella sakaiensis 201-F6, by Shosuke Yoshida and his colleagues from Japan, which has been studied and published in Science this March2. They analyzed the degrading pathways and isolated two kinds of enzymes, PETase and MHETase. The PETase degrades PET into MHET (mono(2-hydroxyethyl) terephthalic acid) and MHETase degrades MHET into TPA (terephthalic acid) and EG (ethylene glycol).

Bottle 1
Bottle 2
Bottle 3
Team Tianjin Project Overview

Our Solution

We synthetize the two genes according to the sequences from NCBI, and our project aims to improve the degrading abilities of the two enzymes, especially the key enzyme, PETase, which degrades macromolecule PET with significantly low rate (60mg PET film (20 × 15 × 0.2 mm) was degraded totally by PETase after 60 days according to the Supplementary Materials3). We applied Protein Engineering stategy to rationally design the enzyme. We designed a Microbial Consortia to degrade PET and its products TPA and EG completely, as well as a Reporting and Regulation system, R-R System to make the expression visible and controllable. When we assay the enzyme activities, we use unconventional method, Cell-Free Protein Synthesis.

Protein Engineering

Protein Engineering


Microbial Consortia

Microbial Consortia


R-R System

Reporting-Regulation System


Cell-Free Protein Expression System

Cell-Free Protein Expression System


Team Tianjin Sponsor Alltech
Team Tianjin Sponsor GenScript
Team Tianjin Sponsor SynbioTech