Difference between revisions of "Team:HUST-China/Description"

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{{HUST-China}}
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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                    <ul class="nav banner-nav">
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                        <li><a href="https://2016.igem.org/Team:HUST-China">HOME</a></li>
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                        <li class="dropdown1"><a class="down-scroll active" href="#">PROJECT</a>
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<div class="column full_size">
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                            <ul class="dropdown2">
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                                <li><a href="https://2016.igem.org/Team:HUST-China/project/background">Background</a></li>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Description">Description</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Proof">Proof of concept</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Demonstrate">Application</a></li>
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<h5>What should this page contain?</h5>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/project/inprovement">Previous improvement</a></li>
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<ul>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Safety">Safety</a></li>
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<li> A clear and concise description of your project.</li>
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                            </ul>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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                        </li>
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<li>References and sources to document your research.</li>
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                        <li class="dropdown1"><a href="#">WETLAB</a>
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<li>Use illustrations and other visual resources to explain your project.</li>
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                            <ul class="dropdown2">
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</ul>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Experiments">Experiments</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Results">Results</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/InterLab">Interlab</a></li>
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</div>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Notebook">Notebook</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li>
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<div class="column full_size" >
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                            </ul>
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                        </li>
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<h5>Advice on writing your Project Description</h5>
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                        <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a>
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                            <ul class="dropdown2">
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<p>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-pro">Prokaryotic circuit</a></li>
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</p>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-euk">Eukaryotic circuit</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-app">Application circuit</a></li>
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<p>
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                            </ul>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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                        </li>
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</p>
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                        <li class="dropdown1"><a class="down-scroll" href="#">PARTS</a>
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                            <ul class="dropdown2">
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</div>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Basic_Part">Basic part</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Composite_Part">Composite part</a></li>
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<div class="column half_size" >
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li>
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                            </ul>
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<h5>References</h5>
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                        </li>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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                        <li class="dropdown1"><a class="down-scroll" href="#">HUMAN PRACTICES</a>
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                            <ul class="dropdown2">
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</div>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Integrated_Practices">Integrated HP</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li>
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                            </ul>
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<div class="column half_size" >
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                        </li>
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<h5>Inspiration</h5>
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                        <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a>
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<p>See how other teams have described and presented their projects: </p>
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                            <ul class="dropdown2">
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Team">Team Roster</a></li>
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<ul>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
−
<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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                            </ul>
−
<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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                        </li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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                    </ul>
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</ul>
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 +
    <div class="container-fluid member-head">
 +
        <div class="container">
 +
            <h2 class="text-center">Description</h2>
 +
        </div>
 +
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 +
    <div class="container-fluid">
 +
        <div class="text-content container" id="text-content">
 +
            <div class="ref"></div>
 +
            <article>
 +
            <p>iGEM is a stage for synthetic biology researchers to build a different world. Our team was inspired by previous iGEM teams and their projects. This year, we are eager to participate and contribute more.</p>
 +
                <!-- h2一级标题 -->
 +
                <!-- 文字 -->
 +
                <p>
 +
                    <strong>At the beginning</strong>,  we learned about previous projects from the websites, and we realized that, when dealing with real-world problems, issues like gene regulation, expression efficiency and system robustness are all important. When creating a great functional prototype, one should pay attention to all the details. And some of the new comers to synthetic biology may find it difficult to deal with. So we put it in our way: why not provide some validated gene expression regulation kits to iGEMers so that they no longer need to worry about building circuit, but focus on the key problem.
 +
                </p>
 +
                <p>
 +
                  We came up with different versions of gene expression circuits: lambda bacteriophage, kinases reaction pathway, ribo-switch, RNAi and so on. We made efforts on the circuit construction and valid characterization data while users mainly focus on the input and output which are highly concerned with real-world problems. In this manner, our kit can serve as a useful tool to save their time and energy. On the whole, our theme is to offer bricks to help others build their own project.
 +
                </p>
 +
                <p>And this summer we <strong>stepped a little forward</strong> to our goals. We worked out two versions of gene expression switch: a prokaryote tri-stable version derived from bacteriophage lambda and eukaryote bi-stable version based on ABA-response pathway.
 +
                </p>
 +
                <p>
 +
                    <strong>In the tri-stable gene expression switch,</strong> users should define two input and related output gene signals. The logic gate below can illustrate the circuit better:
 +
                </p>
 +
                <img src="https://static.igem.org/mediawiki/2016/7/70/T--HUST-China--Logic-gate.jpg" class="img-responsive" alt="">
 +
                <p>The circuit can rapidly reach its stable state because of positive feedback. And users can adjust its threshold too.</p>  
 +
<br><a href="https://2016.igem.org/Team:HUST-China/Proof#location_Pro_ver" style="text-decoration:none"><button type="button" class="btn btn-info center-block">Click to know more about the circuit</button></a>  
 +
                <!-- h3二级标题 -->
 +
                <!--                <h3>SubHeading</h3>
 +
                <p>dfwef</p>
 +
                <h3>Hello world</h3>
 +
                <p>fdwfewf</p> -->
 +
                <p><strong>For more application examples:</strong></p>
 +
                <p>We provided a solution to lactose intolerance based on this toolkit.</p>
 +
                <p>The input of signals are achieved by two promoters: plac (lactose inducible) and patp2 (base inducible). As for the output, we set the gene 1 as iLDH and gene 2 as beta-galactosidase. iLDH can transform lactic acid into pyruvate whileβ-galactosidase can degrade lactose. In this way, the system can provide a promising way for the treatment of lactose intolerance.</p>
 +
                <img src="https://static.igem.org/mediawiki/2016/c/c8/T--HUST-China--description-app.png" alt="">
 +
                <p><strong>In our bi-stable switch</strong>, users can define an open signal and off signal to control the expression of target gene. The most attractive feature of the circuit is its switch efficiency, the mechanism of which is cascade reaction. </p>  
 +
                <img src="https://static.igem.org/mediawiki/2016/8/83/T--HUST-China--description-logic.png" class="img-responsive" alt="">
 +
            <br><a href="https://2016.igem.org/Team:HUST-China/Proof#location_Euk_ver" style="text-decoration:none"><button type="button" class="btn btn-info center-block"> Click to know more about the circuit</button></a>  
 +
            </article>
 +
        </div>
 +
    </div>
 +
    <div class="returnTop">
 +
        <img src="https://static.igem.org/mediawiki/2016/f/f0/T--HUST-China--returnTop.png" alt="">
 +
    </div>
 +
</body>
  
 
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Latest revision as of 23:26, 19 October 2016

Description

Description

iGEM is a stage for synthetic biology researchers to build a different world. Our team was inspired by previous iGEM teams and their projects. This year, we are eager to participate and contribute more.

At the beginning, we learned about previous projects from the websites, and we realized that, when dealing with real-world problems, issues like gene regulation, expression efficiency and system robustness are all important. When creating a great functional prototype, one should pay attention to all the details. And some of the new comers to synthetic biology may find it difficult to deal with. So we put it in our way: why not provide some validated gene expression regulation kits to iGEMers so that they no longer need to worry about building circuit, but focus on the key problem.

We came up with different versions of gene expression circuits: lambda bacteriophage, kinases reaction pathway, ribo-switch, RNAi and so on. We made efforts on the circuit construction and valid characterization data while users mainly focus on the input and output which are highly concerned with real-world problems. In this manner, our kit can serve as a useful tool to save their time and energy. On the whole, our theme is to offer bricks to help others build their own project.

And this summer we stepped a little forward to our goals. We worked out two versions of gene expression switch: a prokaryote tri-stable version derived from bacteriophage lambda and eukaryote bi-stable version based on ABA-response pathway.

In the tri-stable gene expression switch, users should define two input and related output gene signals. The logic gate below can illustrate the circuit better:

The circuit can rapidly reach its stable state because of positive feedback. And users can adjust its threshold too.


For more application examples:

We provided a solution to lactose intolerance based on this toolkit.

The input of signals are achieved by two promoters: plac (lactose inducible) and patp2 (base inducible). As for the output, we set the gene 1 as iLDH and gene 2 as beta-galactosidase. iLDH can transform lactic acid into pyruvate whileβ-galactosidase can degrade lactose. In this way, the system can provide a promising way for the treatment of lactose intolerance.

In our bi-stable switch, users can define an open signal and off signal to control the expression of target gene. The most attractive feature of the circuit is its switch efficiency, the mechanism of which is cascade reaction.