Difference between revisions of "Team:HUST-China/Composite Part"

 
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                 <h2>Eukaryotic Version of Signal Filter</h2>
 
                 <h2>Eukaryotic Version of Signal Filter</h2>
 
                 <img src="https://static.igem.org/mediawiki/2016/1/1e/T--HUST-China--Description-Fig-prokaryote.png" alt="">
 
                 <img src="https://static.igem.org/mediawiki/2016/1/1e/T--HUST-China--Description-Fig-prokaryote.png" alt="">
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                 <h3>Bi-stable Function</h3>
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                 <h3>Bi-stable Function：</h3>
 
                 <img src="https://static.igem.org/mediawiki/2016/4/45/T--HUST-China--Experiments-Fig4-1.png" alt="">
 
                 <img src="https://static.igem.org/mediawiki/2016/4/45/T--HUST-China--Experiments-Fig4-1.png" alt="">
 
                 <img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt="">
 
                 <img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt="">

Latest revision as of 23:32, 19 October 2016

Parts

Composite Part

Following documented composite parts are respectively from three circuits this year:

Prokaryotic Version of Signal Filter

Protein&Promoter Interaction:

Part Number Name Description
BBa_K2036013 RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CII’s interaction with pRE.
BBa_K2036015 RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag Tandem of CII amplifies its function.

Protein&Protein Interaction:

Part Number Name Description
BBa_K2036014 RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag To characterize CIII’s interaction with Ftsh.

Tri-stable Function:

Part Number Name Description
BBa_K2036020 RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To construct the verification circuit.
BBa_K2036027 pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag To verify the function of the whole circuit with T7 and ptrp sensing the signals.

Eukaryotic Version of Signal Filter

Bi-stable Function：

Part Number Name Description
BBa_K2036026 PP2CA-TTADH1 To construct the verification circuit.
BBa_K2036033 PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc To construct the verification circuit.
BBa_K2036029 PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-Prd29A-Kozak-GFP-LVAssrAtag To characterize PP2CA’s interaction with ABF2,which means the whole circuit functional verification.

Application of Signal Filter

Part Number Name Description
BBa_K2036024 placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase The whole circuit of the application for lactose intolerance.