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− | <div class="column full_size judges-will-not-evaluate"> | + | <body> |
− | <h3>★ ALERT! </h3> | + | <header class="header"> |
− | <p>This page is used by the judges to evaluate your team for the <a href="https://2016.igem.org/Judging/Awards#Special_Prizes">Composite Part special prize</a>. </p> | + | <div class="navbar-logo"> |
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− | <p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p> | + | <span class="menu">Menu</span> |
− | </div> | + | <div class="banner-top"> |
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− | | + | <li><a href="https://2016.igem.org/Team:HUST-China">HOME</a></li> |
− | | + | <li class="dropdown1"><a class="down-scroll" href="#">PROJECT</a> |
− | | + | <ul class="dropdown2"> |
− | | + | <li><a href="https://2016.igem.org/Team:HUST-China/project/background">Background</a></li> |
− | <div class="column full_size"> | + | <li><a href="https://2016.igem.org/Team:HUST-China/Description">Description</a></li> |
− | | + | <li><a href="https://2016.igem.org/Team:HUST-China/Proof">Proof of concept</a></li> |
− | | + | <li><a href="https://2016.igem.org/Team:HUST-China/Demonstrate">Application</a></li> |
− | | + | <li><a href="https://2016.igem.org/Team:HUST-China/project/inprovement">Previous improvement</a></li> |
− | <p> | + | <li><a href="https://2016.igem.org/Team:HUST-China/Safety">Safety</a></li> |
− | A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
| + | </ul> |
− | </p> | + | </li> |
− | | + | <li class="dropdown1"><a href="#">WETLAB</a> |
− | <p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p> | + | <ul class="dropdown2"> |
− | | + | <li><a href="https://2016.igem.org/Team:HUST-China/Experiments">Experiments</a></li> |
− | | + | <li><a href="https://2016.igem.org/Team:HUST-China/Results">Results</a></li> |
− | | + | <li><a href="https://2016.igem.org/Team:HUST-China/InterLab">Interlab</a></li> |
− | <div class="highlight"> | + | <li><a href="https://2016.igem.org/Team:HUST-China/Notebook">Notebook</a></li> |
− | <h4>Note</h4> | + | <li><a href="https://2016.igem.org/Team:HUST-China/wetlab/protocols">Protocols</a></li> |
− | <p>This page should list all the composite parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p> | + | </ul> |
− | | + | </li> |
− | </div> | + | <li class="dropdown1"><a class="down-scroll" href="#">MODELING</a> |
− | | + | <ul class="dropdown2"> |
− | </div> | + | <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-pro">Prokaryotic circuit</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-euk">Eukaryotic circuit</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-app">Application circuit</a></li> |
| + | </ul> |
| + | </li> |
| + | <li class="dropdown1"><a class="down-scroll active" href="#">PARTS</a> |
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| + | <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Basic_Part">Basic part</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Composite_Part">Composite part</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li> |
| + | </ul> |
| + | </li> |
| + | <li class="dropdown1"><a class="down-scroll" href="#">HUMAN PRACTICES</a> |
| + | <ul class="dropdown2"> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Integrated_Practices">Integrated HP</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li> |
| + | </ul> |
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| + | <li class="dropdown1"><a class="down-scroll" href="#">TEAM</a> |
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| + | <li><a href="https://2016.igem.org/Team:HUST-China/Team">Team Roster</a></li> |
| + | <li><a href="https://2016.igem.org/Team:HUST-China/Attributions">Attributions</a></li> |
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| + | <h2 class="text-center">Composite Part</h2> |
| + | <p style="color:#fff;margin-bottom:10px;text-align:center;">Following documented composite parts are respectively from three circuits this year:</p> |
| + | </div> |
| + | </div> |
| + | <div class="container-fluid"> |
| + | <div class="text-content container" id="text-content"> |
| + | <div class="ref"></div> |
| + | <article> |
| + | <h2>Prokaryotic Version of Signal Filter</h2> |
| + | <img src="https://static.igem.org/mediawiki/2016/8/88/T--HUST-China--Description-Fig-Eukaryote.png" alt=""> |
| + | <h3>Protein&Promoter Interaction:</h3> |
| + | <img src="https://static.igem.org/mediawiki/2016/0/06/T--HUST-China--Experiments-Fig10.png" alt=""> |
| + | <table align="center" border="1"> |
| + | <tr> |
| + | <td>Part Number</td> |
| + | <td>Name</td> |
| + | <td>Description</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036013"> BBa_K2036013 </a></td> |
| + | <td> RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag </td> |
| + | <td> To characterize CII’s interaction with pRE.</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036015"> BBa_K2036015 </a></td> |
| + | <td> RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag </td> |
| + | <td> Tandem of CII amplifies its function.</td> |
| + | </tr> |
| + | </table> |
| + | <h3>Protein&Protein Interaction:</h3> |
| + | <img src="https://static.igem.org/mediawiki/2016/f/f9/T--HUST-China--Experiments-Fig6.png" alt=""> |
| + | <table align="center" border="1"> |
| + | <tr> |
| + | <td>Part Number</td> |
| + | <td>Name</td> |
| + | <td>Description</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036014"> BBa_K2036014 </a></td> |
| + | <td> RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag </td> |
| + | <td> To characterize CIII’s interaction with Ftsh.</td> |
| + | </tr> |
| + | </table> |
| + | <h3>Tri-stable Function:</h3> |
| + | <img src="https://static.igem.org/mediawiki/2016/1/17/T--HUST-China--Experiments-Fig14.png" alt=""> |
| + | <table align="center" border="1"> |
| + | <tr> |
| + | <td>Part Number</td> |
| + | <td>Name</td> |
| + | <td>Description</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036020"> BBa_K2036020 </a></td> |
| + | <td> RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td> |
| + | <td> To construct the verification circuit.</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036027"> BBa_K2036027 </a></td> |
| + | <td>pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag </td> |
| + | <td> To verify the function of the whole circuit with T7 and ptrp sensing the signals.</td> |
| + | </tr> |
| + | </table> |
| + | <h2>Eukaryotic Version of Signal Filter</h2> |
| + | <img src="https://static.igem.org/mediawiki/2016/1/1e/T--HUST-China--Description-Fig-prokaryote.png" alt=""> |
| + | <h3>Bi-stable Function:</h3> |
| + | <img src="https://static.igem.org/mediawiki/2016/4/45/T--HUST-China--Experiments-Fig4-1.png" alt=""> |
| + | <img src="https://static.igem.org/mediawiki/2016/b/be/T--HUST-China--Experiments-Fig4-2.png" alt=""> |
| + | <table align="center" border="1"> |
| + | <tr> |
| + | <td>Part Number</td> |
| + | <td>Name</td> |
| + | <td>Description</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036026"> BBa_K2036026 </a></td> |
| + | <td> PP2CA-TTADH1 </td> |
| + | <td> To construct the verification circuit.</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036033"> BBa_K2036033 </a></td> |
| + | <td> PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc </td> |
| + | <td> To construct the verification circuit. </td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036029"> BBa_K2036029 </a></td> |
| + | <td>PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-Prd29A-Kozak-GFP-LVAssrAtag </td> |
| + | <td> To characterize PP2CA’s interaction with ABF2,which means the whole circuit functional verification. </td> |
| + | </tr> |
| + | </table> |
| + | <h2>Application of Signal Filter</h2> |
| + | <img src="https://static.igem.org/mediawiki/2016/a/a3/T--HUST-China--modeling-application.png" alt=""> |
| + | <table align="center" border="1"> |
| + | <tr> |
| + | <td>Part Number</td> |
| + | <td>Name</td> |
| + | <td>Description</td> |
| + | </tr> |
| + | <tr> |
| + | <td><a href="http://parts.igem.org/Part:BBa_K2036024"> BBa_K2036024 </a></td> |
| + | <td> placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase </td> |
| + | <td> The whole circuit of the application for lactose intolerance.</td> |
| + | </tr> |
| + | </table> |
| + | </article> |
| + | </div> |
| + | </div> |
| + | <div class="returnTop"> |
| + | <img src="https://static.igem.org/mediawiki/2016/f/f0/T--HUST-China--returnTop.png" alt=""> |
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