Difference between revisions of "Team:HUST-China/Parts"

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{{HUST-China}}
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model">Overview</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-pro">Prokaryotic circuit</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-euk">Eukaryotic circuit</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Model/model-app">Application circuit</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Parts">Summary</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Basic_Part">Basic part</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Composite_Part">Composite part</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Part_Collection">Part Collection</a></li>
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                            </ul>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Integrated_Practices">Integrated HP</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Collaborations">Collaborations</a></li>
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                                <li><a href="https://2016.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
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                            </ul>
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                        </li>
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                    </ul>
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    <div class="container-fluid member-head">
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        <div class="container">
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            <h2 class="text-center">Parts Summary</h2>
 +
        </div>
 +
    </div>
 +
    <div class="container-fluid">
 +
        <div class="text-content" id="text-content">
 +
            <div class="ref"></div>
 +
            <article>
 +
                <div class="col-lg-8 col-lg-offset-2">
 +
                    <p>Welcome to our achievement page of parts! This year, we focus on gene expression regulation in a track of foundational advance. Thus, well-characterized parts are important in our toolkit and very essential to our end-users.</p>
 +
                    <p>The two versions of our signal filter and prokaryotic application circuit well support our project and are validly characterized and documented under our efforts. We submitted 36 parts totally to the Registry, 25 basic parts, 9 composite parts and 2 construction intermediates.</p>
 +
                    <p>Sincerely,we hope these parts can contribute to the Registry and light up the future of synthetic biology creation. </p>
 +
                    <p>Parts are classified in the following four catalogs, click Part Number to see more details:</p>
 +
                </div>
 +
            </article>
 +
            <section class="liquid-slider" id="main-slider">
 +
                <div>
 +
                    <h2 class="title">Prokaryote circuit</h2>
 +
                    <p>
 +
                        <table border="1">
 +
<tr>
 +
<td>Part Number</td>
 +
  <td>Type</td>
 +
  <td>Description</td>
 +
</tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036001"> BBa_K2036001 </a></td>
 +
                                <td> basic </td>
 +
                                <td> CIII </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036000"> BBa_K2036000</a></td>
 +
                                <td> basic </td>
 +
                                <td> RBS-CII </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036006"> BBa_K2036006</a></td>
 +
                                <td> basic </td>
 +
                                <td> RBS-CIII </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036007"> BBa_K2036007</a></td>
 +
                                <td> basic </td>
 +
                                <td> RBS-CII-RBS-CII </td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036008"> BBa_K2036008</a></td>
 +
                                <td>basic</td>
 +
                                <td>RBS-CIII-RBS-CIII</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036009"> BBa_K2036009</a></td>
 +
                                <td>basic</td>
 +
                                <td>pRM-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036010"> BBa_K2036010</a></td>
 +
                                <td>basic</td>
 +
                                <td>Cro-TT-pRM-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036011"> BBa_K2036011</a></td>
 +
                                <td>basic</td>
 +
                                <td>pRE-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036012"> BBa_K2036012</a></td>
 +
                                <td>basic</td>
 +
                                <td>RBS-CII-TT</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036016"> BBa_K2036016</a></td>
 +
                                <td>basic</td>
 +
                                <td>pR-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036017"> BBa_K2036017</a></td>
 +
                                <td>basic</td>
 +
                                <td>CI-TT-pR-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036018"> BBa_K2036018</a></td>
 +
                                <td>basic</td>
 +
                                <td>pRE-RBS-Cro-RBS-CII-TT-ptrp</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036019"> BBa_K2036019</a></td>
 +
                                <td>basic</td>
 +
                                <td>RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036032"> BBa_K2036032</a></td>
 +
                                <td>basic</td>
 +
                                <td>CII-TT-pRE-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036020"> BBa_K2036020</a></td>
 +
                                <td>composite</td>
 +
                                <td>RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036027"> BBa_K2036027</a></td>
 +
                                <td>composite</td>
 +
                                <td>pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036013"> BBa_K2036013</a></td>
 +
                                <td>composite</td>
 +
                                <td>RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036014"> BBa_K2036014</a></td>
 +
                                <td>composite</td>
 +
                                <td>RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036015"> BBa_K2036015</a></td>
 +
                                <td>composite</td>
 +
                                <td>RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                        </table>
 +
                    </p>
 +
                </div>
 +
                <div>
 +
                    <h2 class="title">Eukaryote circuit</h2>
 +
                    <p>
 +
                        <table border="1">
 +
<tr>
 +
<td>Part Number</td>
 +
  <td>Type</td>
 +
  <td>Description</td>
 +
</tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036003"> BBa_K2036003</a></td>
 +
                                <td>basic</td>
 +
                                <td>SnRK2.2</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036004"> BBa_K2036004</a></td>
 +
                                <td>basic</td>
 +
                                <td>ABF2</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036005"> BBa_K2036005</a></td>
 +
                                <td>basic</td>
 +
                                <td>PP2CA</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036002"> BBa_K2036002</a></td>
 +
                                <td>basic</td>
 +
                                <td>Prd29A</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036031"> BBa_K2036031</a></td>
 +
                                <td>basic</td>
 +
                                <td>PP2CA-TTADH1-pCyc</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036025"> BBa_K2036025</a></td>
 +
                                <td>basic</td>
 +
                                <td>Kozak-Snrk2.2-TTADH1-pCyc</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036029"> BBa_K2036029</a></td>
 +
                                <td>basic</td>
 +
                                <td>Kozak-ABF2-TTADH1-Prd29A-Kozak-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036033"> BBa_K2036033</a></td>
 +
                                <td>composite</td>
 +
                                <td>PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036026"> BBa_K2036026</a></td>
 +
                                <td>composite</td>
 +
                                <td>PP2CA-TTADH1</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036030"> BBa_K2036030</a></td>
 +
                                <td>composite</td>
 +
                                <td>PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-Prd29A-Kozak-GFP-LVAssrAtag</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_S05325"> BBa_S05325</a></td>
 +
                                <td>Construction Intermediate</td>
 +
                                <td>PP2CA-TTADH1-pCyc</td>
 +
                            </tr>
 +
                        </table>
 +
                    </p>
 +
                </div>
 +
                <div>
 +
                    <h2 class="title">Application circuit</h2>
 +
                    <p>
 +
                        <table border="1">
 +
                            <tr>
 +
<td>Part Number</td>
 +
<td>Type</td>
 +
  <td>Description</td>
 +
</tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036021"> BBa_K2036021</a></td>
 +
                                <td>basic</td>
 +
                                <td>placm-pRE-RBS-Cro-RBS-CII-TT</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036022"> BBa_K2036022</a></td>
 +
                                <td>basic</td>
 +
                                <td>patp2-RBS-CI-TT-pR-RBS-CIII</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036023"> BBa_K2036023</a></td>
 +
                                <td>basic</td>
 +
                                <td>RBS-iLDH-TT-pRM-RBS-beta-galactosidase</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036028"> BBa_K2036028</a></td>
 +
                                <td>basic</td>
 +
                                <td>placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_K2036024"> BBa_K2036024</a></td>
 +
                                <td>composite</td>
 +
                                <td>placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase</td>
 +
                            </tr>
 +
                            <tr>
 +
                                <td><a href="http://parts.igem.org/Part:BBa_S05333"> BBa_S05333</a></td>
 +
                                <td>Construction Intermediate</td>
 +
                                <td>placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII</td>
 +
                            </tr>
 +
                        </table>
 +
                    </p>
 +
                </div>
 +
                <div>
 +
                    <h2 class="title">Improvements</h2>
 +
                    <p>
 +
                        <table border="1">
 +
<tr>
 +
<td>Part Number</td>
 +
  <td>Description</td>
 +
  <td>Function</td>
 +
</tr>
 +
<tr>
 +
<th colspan="3">Flocculation</th>
 +
</tr>
  
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592000"> BBa_K1592000 </a></td>
 +
  <td> LIP2 prepro(signal peptide) </td>
 +
  <td> As a signal peptide to secrete Mcfp-3 and Si-tag out of the membrane</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592001"> BBa_K1592001 </a></td>
 +
  <td> Mytilus californianus foot protein 3(Mcfp3) variant 3 </td>
 +
  <td>Vicious protein to help bind sands together.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592003"> BBa_K1592003 </a></td>
 +
  <td> Mcfp3 with LIP2 prepro </td>
 +
  <td> Fusion protein to be secreted out of the membrane to bind sands together</td>
 +
</tr>
 +
<tr>
 +
<th colspan="3">Cementation</th></tr>
 +
<tr>
 +
<td>Part number</td>
 +
  <td>Description</td>
 +
  <td>Function</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592002"> BBa_K1592002 </a></td>
 +
  <td> Yarrowia lipolytica cell wall protein 3 </td>
 +
  <td> Anchor protein to help display Si-tag on the cell wall.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592007"> BBa_K1592007 </a></td>
 +
  <td>LIP prepro + E. coli ribosomal protein L2 (1-60aa) + YLcwp3 Fusion </td>
 +
  <td>Fusion protein to be displayed on the cell wall to help immobilized on sand or solid surface</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592008"> BBa_K1592008 </a></td>
 +
  <td> LIP prepro + E. coli ribosomal protein L2 (61-202aa) + YLcwp3 Fusion </td>
 +
  <td>The same as above, but different domain combinations of Si-tag show different cementation intensity.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592009"> BBa_K1592009 </a></td>
 +
  <td> LIP2 prepro + E. coli ribosomal protein L2 (203-273aa) + YLcwp3 Fusion</td>
 +
  <td>The same as above, but different domain combinations of Si-tag show different cementation intensity.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592010"> BBa_K1592010</a></td>
 +
  <td>LIP2 prepro + E. coli ribosomal protein L2 (1-202aa)+ YLcwp3 Fusion </td>
 +
  <td>The same as above, but different domain combinations of Si-tag show different cementation intensity.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592011"> BBa_K1592011</a></td>
 +
  <td> LIP prepro + E. coli ribosomal protein L2 (61-273aa) + YLcwp3 Fusion</td>
 +
  <td>The same as above, but different domain combinations of Si-tag show different cementation intensity.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592012"> BBa_K1592012</a></td>
 +
  <td> LIP prepro + E. coli ribosomal protein L2 (1-60,203-273aa) + YLcwp3 Fusion</td>
 +
  <td>The same as above, but different domain combinations of Si-tag show different cementation intensity.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592013"> BBa_K1592013</a></td>
 +
  <td> LIP prepro + E. coli ribosomal protein L2 (1-60,GS linker,202-273aa) + YLcwp3</td>
 +
  <td>The same as above, but different domain combinations of Si-tag show different cementation intensity.</td>
 +
</tr>
 +
<tr>
 +
  <td><a href="http://parts.igem.org/Part:BBa_K1592014"> BBa_K1592014</a></td>
 +
  <td> LIP prepro + E. coli ribosomal protein L2 (1-273aa) + YLcwp3</td>
 +
  <td>The same as above, but different domain combinations of Si-tag show different cementation intensity.</td>
 +
</tr>
  
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                    </p>
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                </div>
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+
            </section>
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
        </div>
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
+
    </div>
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    <div class="returnTop">
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        <img src="https://static.igem.org/mediawiki/2016/f/f0/T--HUST-China--returnTop.png" alt="">
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<h5>Note</h5>
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    });
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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    </script>
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
+
−
<ul>
+
−
<li>Part Name</li>
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−
<li>Part type</li>
+
−
<li>Creator</li>
+
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
+
−
<li>Long Description (Longer description of what the DNA does)</li>
+
−
<li>Design considerations</li>
+
−
</ul>
+
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+
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<p>
+
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h5>Part Table </h5>
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<groupparts>iGEM2016 Example</groupparts>
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Latest revision as of 00:04, 20 October 2016

Parts

Parts Summary

Welcome to our achievement page of parts! This year, we focus on gene expression regulation in a track of foundational advance. Thus, well-characterized parts are important in our toolkit and very essential to our end-users.

The two versions of our signal filter and prokaryotic application circuit well support our project and are validly characterized and documented under our efforts. We submitted 36 parts totally to the Registry, 25 basic parts, 9 composite parts and 2 construction intermediates.

Sincerely,we hope these parts can contribute to the Registry and light up the future of synthetic biology creation. 

Parts are classified in the following four catalogs, click Part Number to see more details:

Prokaryote circuit

Part Number Type Description
BBa_K2036001 basic CIII
BBa_K2036000 basic RBS-CII
BBa_K2036006 basic RBS-CIII
BBa_K2036007 basic RBS-CII-RBS-CII
BBa_K2036008 basic RBS-CIII-RBS-CIII
BBa_K2036009 basic pRM-GFP-LVAssrAtag
BBa_K2036010 basic Cro-TT-pRM-RBS-GFP-LVAssrAtag
BBa_K2036011 basic pRE-GFP-LVAssrAtag
BBa_K2036012 basic RBS-CII-TT
BBa_K2036016 basic pR-GFP-LVAssrAtag
BBa_K2036017 basic CI-TT-pR-RBS-GFP-LVAssrAtag
BBa_K2036018 basic pRE-RBS-Cro-RBS-CII-TT-ptrp
BBa_K2036019 basic RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
BBa_K2036032 basic CII-TT-pRE-RBS-GFP-LVAssrAtag
BBa_K2036020 composite RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
BBa_K2036027 composite pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
BBa_K2036013 composite RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag
BBa_K2036014 composite RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag
BBa_K2036015 composite RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag

Eukaryote circuit

Part Number Type Description
BBa_K2036003 basic SnRK2.2
BBa_K2036004 basic ABF2
BBa_K2036005 basic PP2CA
BBa_K2036002 basic Prd29A
BBa_K2036031 basic PP2CA-TTADH1-pCyc
BBa_K2036025 basic Kozak-Snrk2.2-TTADH1-pCyc
BBa_K2036029 basic Kozak-ABF2-TTADH1-Prd29A-Kozak-GFP-LVAssrAtag
BBa_K2036033 composite PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc
BBa_K2036026 composite PP2CA-TTADH1
BBa_K2036030 composite PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-Prd29A-Kozak-GFP-LVAssrAtag
BBa_S05325 Construction Intermediate PP2CA-TTADH1-pCyc

Application circuit

Part Number Type Description
BBa_K2036021 basic placm-pRE-RBS-Cro-RBS-CII-TT
BBa_K2036022 basic patp2-RBS-CI-TT-pR-RBS-CIII
BBa_K2036023 basic RBS-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K2036028 basic placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K2036024 composite placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_S05333 Construction Intermediate placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII

Improvements

Part Number Description Function
Flocculation
BBa_K1592000 LIP2 prepro(signal peptide) As a signal peptide to secrete Mcfp-3 and Si-tag out of the membrane
BBa_K1592001 Mytilus californianus foot protein 3(Mcfp3) variant 3 Vicious protein to help bind sands together.
BBa_K1592003 Mcfp3 with LIP2 prepro Fusion protein to be secreted out of the membrane to bind sands together
Cementation
Part number Description Function
BBa_K1592002 Yarrowia lipolytica cell wall protein 3 Anchor protein to help display Si-tag on the cell wall.
BBa_K1592007 LIP prepro + E. coli ribosomal protein L2 (1-60aa) + YLcwp3 Fusion Fusion protein to be displayed on the cell wall to help immobilized on sand or solid surface
BBa_K1592008 LIP prepro + E. coli ribosomal protein L2 (61-202aa) + YLcwp3 Fusion The same as above, but different domain combinations of Si-tag show different cementation intensity.
BBa_K1592009 LIP2 prepro + E. coli ribosomal protein L2 (203-273aa) + YLcwp3 Fusion The same as above, but different domain combinations of Si-tag show different cementation intensity.
BBa_K1592010 LIP2 prepro + E. coli ribosomal protein L2 (1-202aa)+ YLcwp3 Fusion The same as above, but different domain combinations of Si-tag show different cementation intensity.
BBa_K1592011 LIP prepro + E. coli ribosomal protein L2 (61-273aa) + YLcwp3 Fusion The same as above, but different domain combinations of Si-tag show different cementation intensity.
BBa_K1592012 LIP prepro + E. coli ribosomal protein L2 (1-60,203-273aa) + YLcwp3 Fusion The same as above, but different domain combinations of Si-tag show different cementation intensity.
BBa_K1592013 LIP prepro + E. coli ribosomal protein L2 (1-60,GS linker,202-273aa) + YLcwp3 The same as above, but different domain combinations of Si-tag show different cementation intensity.
BBa_K1592014 LIP prepro + E. coli ribosomal protein L2 (1-273aa) + YLcwp3 The same as above, but different domain combinations of Si-tag show different cementation intensity.